terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Reconstructing ancient microbial fermentation genomes from the wine residues of Herod, Roman king of Judea

Reconstructing ancient microbial fermentation genomes from the wine residues of Herod, Roman king of Judea

Abstract

The fortress of the Herodium, built towards the end of the first century BCE/ante Cristo, on the orders of Herod the Great, Roman client king of Judea, attests the expansion of Roman influence in the eastern Mediterranean. During archaeological excavations of the Herodium in 2017[1], a winery was discovered on the ground floor of the palace, with an assortment of clay vessels in situ, including large dolia – clay fermentation vessels each capable of fermenting up to 300-400 L of wine. Thanks to the recent progresses in the field of paleogenomics[2], we could analyse the organic material consistent with grape pomace at the bottom of these vessels, by extracting and sequencing the DNA using shotgun metagenomics and targeted capture, aiming for enrichment of DNA from fermentation associated microbes. After controlling for environmental and modern contamination, we successfully reconstructed and analysed the high-quality metagenome-assembled genomes (MAGs) from a phylogenetic and functional perspective. The phylogenetic signal of these ancient fermentation bacteria confirmed both their ancient origin and their affiliation to bacteria associated with wine fermentation. Gene functional analysis of the reconstructed fermentation-associated MAGs revealed an assortment of genes typically expected in lactic acid bacteria involved in wine fermentation, as well as genes involved in the production of wine spoilage compounds, especially in species typically viewed today as less desirable bacteria. Overall, our analysis brings a new appreciation of winemaking in Roman Judea and enriches our understanding of Roman accounts of flavouring wine with different herbs and aromatics, which may have been performed in part to mask the off-flavour compounds produced by bacterial wine spoilage genes.

Acknowledgements:

Werner Siemens Foundation (PALEOBIOTECHNOLOGY, funding M.B. and C.W.) and Deutsche Forschungsgemeinschaft (Balance of the Microverse, EXC 2051 #390713860, funding M.B. and C.W.)

References:

  1. Porat, R., Kalman, Y., Chachy, R., terem, shulamit, Bar-Natan, R., Ecker, A., Ben-Gedalya, T., Drori, E., & Weiss, E. (2018). Herod’s Royal Winery and Wine Storage Facility in the Outer Structure of the Mountain Palace-Fortress at Herodium. Qadmoniot (156, 106–1)
  2. Orlando, L., Allaby, R., Skoglund, P., Der Sarkissian, C., Stockhammer, P. W., Ávila-Arcos, M. C., Fu, Q., Krause, J., Willerslev, E., Stone, A. C., & Warinner, C. (2021). Ancient DNA analysis. Nature Reviews Methods Primers, 1(1), 14, DOI 10.1038/s43586-020-00011-0

DOI:

Publication date: October 3, 2023

Issue: ICGWS 2023

Type: Article

Authors

Maxime Borry1,2, Tziona Ben Gedalya3, Herodion Expedition4, Alexander Herbig1, Christina Warinner1,5,6

1Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany
2Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute, Jena, Germany
3Eastern R&D Center, Ariel University, Ariel, Israel
4Hebrew University
5Faculty of Biological Sciences, Friedrich-Schiller University, Jena, Germany
6Department of Anthropology, Harvard University, Cambridge, MA, USA

Contact the author*

Keywords

roman, herod, judea, paleogenomics, metagenomics, genome, bacteria, ancient DNA

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Yeast mannoprotein characterization and their effect on Oenococcus oeni and malolactic fermentation

Mannoproteins are released at the end of alcoholic fermentation due to yeast autolysis [1]. It has been described a positive effect of these molecules on lactic acid bacteria growth [2]. The main objective of this work was the characterization of different mannoproteins extracted from active dry yeast (ADY) and the assessment of their effect on Oenococcus oeni and malolactic fermentation (MLF).

New food trend ahead? Highlighting the nutritional benefits of grapevine leaves

The wine industry produces an enormous amount of waste every year. A wider inclusion of disregarded by-products in the human diet or its use as a source of bioactive compounds is a good strategy for reducing waste. It will not only introduce an added value to a waste product but also come upon the European Union and United Nations’ demands towards more sustainable agricultural approaches and circular economy.

Effect of abiotic stress and grape variety on amino acid and polyamine composition of red grape berries

Vines are exposed to environmental conditions that cause abiotic stress on the plants (drought, nutrient and mineral deficits, salinity, etc.). Polyamines are growth regulators involved in various physiological processes, as in abiotic plant stress responses. Stressful conditions can modify grape’s composition, and in this work, we have focused on studying the effect of abiotic stress on the composition of polyamines and amino acids in grapes. In addition, the effect of grape variety on these compounds has been studied.

Identification of several glycosidic aroma precursors in six varieties of winemaking grapes and assessment of their aroma potential by acid hydrolysis

In winemaking grapes, it is known that most aroma compounds are present as non-volatile precursors, such as glycosidic precursors. In fact, there is strong evidence supporting the connection between the content of aroma precursors and the aromatic quality of wine [1]. Acid hydrolysis is preferred to reveal the aroma potential of winemaking grapes, as it predicts more accurately the chemical rearrangements occurring during fermentation in acidic environments [2]. In this study, a method involving a fast fermentation followed by acid hydrolysis at 75ºC was used to evaluate the accumulation of aroma compounds over time in fractions obtained from six different varieties of winemaking grapes.

Adsorption of tetraconazole by organic residues and vineyard organically-amended soils 

Spain is the country with the largest wine-producing area in the EU and its productivity is largely controlled applying fungicides. However, residues of these compounds can move and contaminate surface and groundwater. The objective of this work was to evaluate the capacity of bioadsorbents from different origin to adsorb and immobilize tetraconazole by themselves or when applied as organic soil amendment, and to prevent soil and water contamination by this fungicide. The adsorption of tetraconazole by 3 organic residues: spent mushroom substrate (SMS), green compost (GC) and vine pruning sawdust (VP), as well as by vineyard soils unamended and amended individually with these residues at 1.5% (w/w) was evaluated using the batch equilibrium technique.