terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Detoxification capacities of heavy metals and pesticides by yeasts 

Detoxification capacities of heavy metals and pesticides by yeasts 

Abstract

Winegrowing is still characterized by the extensive use of chemical fertilizers and plant protection products, despite strong recommendations to limit these practices. A part of these xenobiotics and metals are then found in grape juice and wine, causing a major health concern, as well as negatively affecting the fermentation process. In recent years, there has been renewed interest in non-Saccharomyces yeasts. These species have a wide phenotypic diversity, which would be exploited to broaden the aromatic palette of wines. The potential of some of these species as a bio-protection agent, in pre-fermentation treatment, has also been established.

In addition to these characteristics, the heavy metal sorption capacities of these species are mentioned in the literature. Pre-fermentation treatment of the harvest with non-Saccharomyces (NS) yeasts therefore appears to be an alternative to the use of chemical inputs for the elimination of organic and inorganic xenobiotics from grape musts. However, our knowledge of the detoxification capacities of yeasts and their molecular and biochemical determinant is not yet sufficiently advanced to allow such methods to be developed and put into practice.  In this study, we investigated the detoxifying abilities of fourteen strains of Saccharomyces and non-Saccharomyces yeasts during the first stages of wine fermentation. Fermentations were conducted in synthetic must in the presence or absence of pesticides (mix of twenty pesticides commonly used in the vineyard) / metals (copper). The colorimetric determination of copper concentration throughout fermentation revealed that most Saccharomyces and non-Saccharomyces yeasts detoxify almost all copper in less than 48 hours. Regarding pesticides detoxification, quantified by GC-MS (Dubernet laboratory), a higher variability was observed, with a removal of 0% to 90% of the compound depending on the yeast strain and the pesticide. In conclusion, this study showed the great diversity of the capacity of yeasts to detoxify molecules used in vineyards to fight against diseases and/or parasites and the biotechnological potential that this represents.

DOI:

Publication date: October 3, 2023

Issue: ICGWS 2023

Type: Article

Authors

Tristan Jacqui1,2, Celine Raynal2, Amandine Deroite2, Vincent Bouazza3 , Carole Camarasa1

1SPO, INRAe, Institut Agro Montpellier, Montpellier, France
2LALLEMAND SAS, Blagnac, France

3Laboratoire Dubernet, Narbonne, France

Contact the author*

Keywords

non-Saccharomyces yeasts, wine quality, heavy metals, pesticides, detoxification

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

New crossbreed winegrape genotypes cultivated under rainfed conditions in a semi-arid Mediterranean region

Traditional drought tolerant varieties such as Cabernet Sauvignon, Monastrell, and Syrah [1], have been used as parents in the grapevine breeding program initiated by the Instituto Murciano de Investigación y Desarrollo Agrario y Medioambiental (IMIDA) in 1997 [2]. This work presents the results of evaluating three new genotypes obtained from crosses between ‘Monastrell’ and ‘Cabernet Sauvignon’ (MC16 and MC80) and between ‘Monastrell’ and ‘Syrah’ (MS104), comparing their performance under conditions of water scarcity and high temperatures with that of their respective parental varieties. For this purpose, the six genotypes were cultivated under controlled irrigation conditions (60% ETc) and rainfed conditions.

Photoprotective extracts from agri-food waste to prevent the effect of light in rosé wines 

Light is responsible for adverse reactions in wine including the formation of unpleasant flavors, loss of vitamins or photodegradation of anthocyanins. Among them, the riboflavin degradation leads to the formation of undesirable volatile compounds, known as light-struck taste. These photo-chemical reactions could be avoided by simply using opaque packaging. However, most rosé wines are kept in transparent bottles due to different commercial reasons. Some agri-food waste extracts have been studied for their photoprotective action which turn to be highly correlated with phenolic content [1].

Can soil nitrate explain polyphenol and anthocyanin content in vineyard with similar available soil water regime? 

Nitrogen (N) is quite important nutrient in grapevine development and must quality, but under Mediterranean climatic conditions, available soil water (ASW) during grapevine development can also influence vigour and must quality. The aim was to determine the influence of soil nitrate (NO3-) availability on N foliar, yield, and must quality in vineyards with similar available water holding capacity (AWC). For this purpose, four cv. Tempranillo (Vitis vinifera L.) vineyards were selected. All of them are placed in Uruñuela municipality (La Rioja, Spain), separated less than 2.5 km and in a slope <1 %, in soils with similar soil chemistry properties and with similar rooting depth (ranging between 105 cm and 110 cm).

Nitrogen forms and Iron deficiency: how do Grapevine rootstocks responses change?

Grapevine rootstocks provide protection against environmental biotic and abiotic stresses. Nitrogen (N) and iron (Fe) are growth-limiting factors in many crop plants due to their effects on the chlorophyll and photosynthetic characteristics. Iron nutrition of plants can be significantly affected by different nitrogen forms through altering the uptake ratio of cations and anions, and changing rhizosphere pH. The aim of this study was to investigate the response mechanisms of grapevine rootstocks due to the interaction between different nitrogen forms and iron uptake.

Effect of ultraviolet B radiation on pathogenic molds of grapes

The fungicidal effect of UV-C radiation (100-280 nm wavelength) is well known, but its applicability for the control of pathogenic molds of grapes is conditioned by its effect on the host and by the risks inherent in its handling[1].
As an alternative, the effect in vitro of UV-B radiation (280-315 nm) on the main pathogenic molds of grapes has been studied: Botrytis cinerea, Aspergillus niger, Penicillium expansum and Rhizopus stolonifer.