terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Detoxification capacities of heavy metals and pesticides by yeasts 

Detoxification capacities of heavy metals and pesticides by yeasts 

Abstract

Winegrowing is still characterized by the extensive use of chemical fertilizers and plant protection products, despite strong recommendations to limit these practices. A part of these xenobiotics and metals are then found in grape juice and wine, causing a major health concern, as well as negatively affecting the fermentation process. In recent years, there has been renewed interest in non-Saccharomyces yeasts. These species have a wide phenotypic diversity, which would be exploited to broaden the aromatic palette of wines. The potential of some of these species as a bio-protection agent, in pre-fermentation treatment, has also been established.

In addition to these characteristics, the heavy metal sorption capacities of these species are mentioned in the literature. Pre-fermentation treatment of the harvest with non-Saccharomyces (NS) yeasts therefore appears to be an alternative to the use of chemical inputs for the elimination of organic and inorganic xenobiotics from grape musts. However, our knowledge of the detoxification capacities of yeasts and their molecular and biochemical determinant is not yet sufficiently advanced to allow such methods to be developed and put into practice.  In this study, we investigated the detoxifying abilities of fourteen strains of Saccharomyces and non-Saccharomyces yeasts during the first stages of wine fermentation. Fermentations were conducted in synthetic must in the presence or absence of pesticides (mix of twenty pesticides commonly used in the vineyard) / metals (copper). The colorimetric determination of copper concentration throughout fermentation revealed that most Saccharomyces and non-Saccharomyces yeasts detoxify almost all copper in less than 48 hours. Regarding pesticides detoxification, quantified by GC-MS (Dubernet laboratory), a higher variability was observed, with a removal of 0% to 90% of the compound depending on the yeast strain and the pesticide. In conclusion, this study showed the great diversity of the capacity of yeasts to detoxify molecules used in vineyards to fight against diseases and/or parasites and the biotechnological potential that this represents.

DOI:

Publication date: October 3, 2023

Issue: ICGWS 2023

Type: Article

Authors

Tristan Jacqui1,2, Celine Raynal2, Amandine Deroite2, Vincent Bouazza3 , Carole Camarasa1

1SPO, INRAe, Institut Agro Montpellier, Montpellier, France
2LALLEMAND SAS, Blagnac, France

3Laboratoire Dubernet, Narbonne, France

Contact the author*

Keywords

non-Saccharomyces yeasts, wine quality, heavy metals, pesticides, detoxification

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Comparison of the effects of hormone- and natural-based elicitors on key metabolic pathways in cv. Tempranillo

One of the most important effects of climate change in wine-growing areas is the advance of phenological stages, especially concerning early berry ripening. In the hottest seasons, this results in a lack of synchrony between sugar and phenolic ripeness. In order to cope with this fact, a general effort is being made by researchers and growers aiming at delaying ripening through different strategies. One of the proposed approaches is the application of elicitors. This study aims to assess the effect at the transcriptomic level of application of three elicitors (Vitalfit, Fruitel, and Protone) in Tempranillo.

The potential of some native varieties of Argentina for the production of sparkling wines. Effect of lees contact time 

Grapevine varieties from South-America, commonly known as criollas, originated because of the natural crossbreeding of grapevine varieties brought by the Spaniards. The objective of this work was to evaluate the potential of some varieties to produce sparkling wines considering the effect of lees contact time. The following varieties were used: Moscatel Rosado, Criolla Chica, Pedro Gimenez, Blanca Oval, Canelón, and the European variety Chardonnay (control), planted in the ampelographic collection of EEA Mendoza INTA (Argentina). Pilot-scale vinifications were carried out to obtain the base wines, in 20 L glass containers. The second fermentation was performed through the traditional method.

Valorization of grapevine leaves: screening of polyphenol composition in 50 cultivars

Grapevine leaves are known to contain different polyphenols such as flavonols, catechins and stilbenes, which are known to act as main contributors for plant defense against pathogens (1). While the composition for some major cultivars has been studied, there is lack of systematic comparison about the content of these compounds in the wide ecodiversity of Vitis vinifera cv. Recent advances in Mass Spectrometry-based Metabolomics allow a wider and more sensitive description of these polyphenols, as instance of those present in leaves (2). Such information could help to better explain leaf traits regarding the development of the leaf or to the plant tolerance to a pathogen. Moreover, these compounds offer appealing applications for human health due to their antioxidant activities.

Can yeast cells sense other yeasts beyond competition interactions?

The utilization of non-Saccharomyces yeasts in the wine industry has increased significantly in recent years. Alternative species need commonly be employed in combination with Saccharomyces cerevisiae to avoid stuck fermentation, or microbial spoilage. The employment of more than one yeast starter can lead to interactions between different species with an impact on the outcome of wine fermentation. Previous studies[1] demonstrated that S. cerevisiae elicits transcriptional responses with both shared and species-specific features in co-culture with other yeast species.

Exploring relationships among grapevine chemical and physiological parameters and mycobiome composition under drought stress

Improving our knowledge on biotic and abiotic factors that influence the composition of the grapevine mycobiome is of great agricultural significance, due to potential effects on plant health, productivity, and wine characteristics. Among the various environmental factors affecting the morphological, physiological, biochemical and molecular attributes of grapevine, drought stress is one of the most severe, becoming increasingly an issue worldwide.