terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Can yeast cells sense other yeasts beyond competition interactions?

Can yeast cells sense other yeasts beyond competition interactions?

Abstract

The utilization of non-Saccharomyces yeasts in the wine industry has increased significantly in recent years. Alternative species need commonly be employed in combination with Saccharomyces cerevisiae to avoid stuck fermentation, or microbial spoilage. The employment of more than one yeast starter can lead to interactions between different species with an impact on the outcome of wine fermentation. Previous studies[1] demonstrated that S. cerevisiae elicits transcriptional responses with both shared and species-specific features in co-culture with other yeast species. We tested the hypothesis that extracellular vesicles (EVs) play a role as mediators in these interactions. For this purpose, we exposed S. cerevisiae cultures to EVs from Metschnikowia pulcherrima. Through RNAseq, we evaluated the impact of these EVs on the physiology of S. cerevisiae, comparing the results with the response of S. cerevisiae to metabolically active M. pulcherrima cells under identical conditions. The analysis revealed a significant overlap in the transcriptional responses induced in S. cerevisiae by both M. pulcherrima cells and EVs. Notably, both stimuli upregulated the genes related to glycolysis and ribosomal activity, while repressing autophagic genes. These findings provide evidence that S. cerevisiae actively responds to competing species under conditions resembling those found in winemaking. Furthermore, it offers experimental support for the hypothesis that EVs take part in interspecies recognition.

Acknowledgements: This work was funded by the Spanish Government through grant PID2019-105159RB-I00 funded by MCIN/AEI/10.13039/501100011033, grant BES-2016-077557, and grant PRE2020-093420 funded by MCIN/AEI/10.13039/501100011033 and by “ESF Investing in your future” (training contracts for AM and MM).

References:

1)  Curiel, J.A., Morales, P., Gonzalez, R., Tronchoni, J., 2017. Different non-Saccharomyces yeast species stimulate nutrient consumption in S. cerevisiae mixed cultures. Front. Microbiol. 8, 2121. https://doi.org/10.3389/fmicb.2017.02121.

DOI:

Publication date: October 3, 2023

Issue: ICGWS 2023

Type: Article

Authors

Miguel Mejías Ortiz1*, Ana Mencher1, Jordi Tronchoni2, Ramon Gonzalez1, Pilar Morales1

1Instituto de Ciencias de la Vid y del Vino (CSIC, Gobierno de la Rioja, Universidad de La Rioja), Logroño, La Rioja, Spain
2Universidad Internacional de Valencia, Valencia, Spain

Contact the author*

Keywords

extracellular vesicles, yeast interactions, transcriptomics, winemaking

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Early defoliation positively enhances bioactive composition of berries with no effect on cuticle characteristics

Leaf removal in the fruit-zone has been employed to improve cluster light exposure and ventilation and therefore increase metabolite accumulation and reduce botrytis incidence in berries. When applied before flowering (early defoliation – ED), it can also decrease cluster compactness and regulate yield in high-yielding varieties. This study aimed to evaluate the impact of ED on the physiology and metabolism of Aragonez (syn. Tempranillo) berries along the ripening period. The experiment was set up in 2013 at a commercial vineyard located in the Lisbon winegrowing region.

Addition of glutathione-rich inactivated yeasts to white musts: effects on wine composition and sensory quality

Glutathione plays a key role in preventing some oxidative processes during winemaking. This molecule limits the must enzymatic oxidation, reacts with caffeic acid and generates a colourless compound that prevents subsequent browning. It also has a protective effect on wine aroma, preventing the oxidation of the volatile compounds with a high sensory impact.

Climate change and viticulture in Nordic Countries and the Helsinki area

The first vineyards in Northern Europe were in Denmark in the 15th century, in the southern parts of Sweden and Finland in the 18th century at 55–60 degrees latitude. The grapes grown there have not been made into wine, but the grapes have been eaten at festive tables. The resurgence of viticulture has started with global warming, and currently the total area of viticulture in the Nordic countries, including Norway, is estimated to be 400–500 hectares, most of which is in Denmark. Southern Finland, like all southern parts of Northern Europe, belongs to the cool-cold winegrowing area.

Effects of different soil types and soil management on greenhouse gas emissions 

Soil is important in the carbon cycle and the dynamics of greenhouse gases (CO2, CH4 and N2O). Key soil characteristics, such as organic matter content, texture, structure, pH and microbial activity, play a determining role in GHG emissions[1]. The objective of the study is to delimit different types of soil, with different soil management and to be able to verify the differences in CO2, CH4 and N2O emissions. The study was carried out in a vineyard of Bodegas Campo Viejo in Logroño (La Rioja), whose plant material is Vitis vinifera L. cv. Tempranillo.

Applicability of spectrofluorometry and voltammetry in combination with machine learning approaches for authentication of DOCa Rioja Tempranillo wines

The main objective of the work was to develop a simple, robust and selective analytical tool that allows predicting the authenticity of Tempranillo wines from DOCa Rioja. The techniques of voltammetry and absorbance-transmission and fluorescence excitation emission matrix (A-TEEM) spectroscopy have been applied in combination with machine learning (ML) algorithms to classify red wines from DOCa Rioja according to region (Alavesa, Alta or Oriental) and category (young, crianza or reserva).