terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Metabolomic profiling of heat-stressed grape berries 

Metabolomic profiling of heat-stressed grape berries 

Abstract

The projected rise in mean air temperatures together with the frequency, intensity, and length of heat waves in many wine-growing regions worldwide will deeply impact grape berry development and quality. Several studies have been conducted and a large set of molecular data was produced to better understand the impact of high temperatures on grape berry development and metabolism[1]. According to these data, it is highly likely that the metabolomic dynamics could be strongly modulated by heat stress (HS). Hence, the objective of the present study is to investigate the metabolome profiling on grape berries, exposed or not, to high temperature. We applied HS directly on clusters from V. vinifera L. Cabernet Sauvignon (heat sensitive genotype) and V. vinifera L.  Merlot (heat tolerant genotype) at different developmental stages. HS was applied continuously from 8:00 am to 16:00 pm for up to 10 days in greenhouse. The temperature difference between the HS-treated and control bunches was 9 °C. Berry samples were collected after both short-term and long-term HS treatment and metabolomic analyses were conducted using the untargeted LC-MS approach. Data processing was performed by MS-DIAL 4.94 and MetaboAnalyst 5.0.

Our first set of results highlights metabolites and distinct biochemical pathways impacted by HS, according to the thermotolerance ability of the evaluated cultivars. Our data also underline the temporal dynamics of metabolic responses triggered by HS, highlighting the importance of characterizing these metabolic changes at different time scales.

Acknowledgements: This work is supported by the ANR (PARASOL Project, ANR-20-CE21-0003) and X. Z. PhD thesis is founded by China Scholarship Council. The authors would like to EGFV Materiel-Vegetal team and Dr. Erwan Chavonet for the fruit cutting production.

References:

  1. Lecourieux F. et al. (2017) Dissecting the biochemical and transcriptomic effects of a locally applied heat treatment on developing cabernet sauvignon grape berries. Front Plant Sci 8: 53

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Xi ZHAN1*, Adam ROCHEPEAU2, Cédric CASSAN2, Fatma OUAKED-LECOURIEUX1, Pierre PETRIACQ2, David LECOURIEUX1

1EGFV, University of Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, Villenave d’Ornon, France 
2Bordeaux Metabolome, INRAE Bordeaux Nouvelle Aquitaine, INRAE, Villenave d’Ornon, France

Contact the author*

Keywords

grapevine, berry quality, metabolomics, high temperature, climate change

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Comparison of the effects of hormone- and natural-based elicitors on key metabolic pathways in cv. Tempranillo

One of the most important effects of climate change in wine-growing areas is the advance of phenological stages, especially concerning early berry ripening. In the hottest seasons, this results in a lack of synchrony between sugar and phenolic ripeness. In order to cope with this fact, a general effort is being made by researchers and growers aiming at delaying ripening through different strategies. One of the proposed approaches is the application of elicitors. This study aims to assess the effect at the transcriptomic level of application of three elicitors (Vitalfit, Fruitel, and Protone) in Tempranillo.

Effect of biological control agents on grapevine rhizosphere microbiome and grapevine defenses

Plant diseases are a major obstacle to crop production. The main approaches to battle plant diseases, consist of synthetic chemicals to attack infecting pathogens. However, concerns are increasing about the effects of chemicals in the environment, leading to an increase in the use of biocontrol agents (BCAs), due to their assets, such as, antagonism, and competition. In this study, we tested the hypothesis that the introduction of Bacillus subtilis PTA-271 (Bs PTA-271) and Trichoderma atroviride SC1 (Ta SC1) produce distinctive modifications in the composition and network structure of the grapevine rhizosphere microbial community, as well as grapevine induced defenses.

White grape must processed by UHPH as an alternative to SO2 addition: Effect on the phenolic composition in three varieties

The quantity and distribution of polyphenols in musts play a fundamental role in the white winemaking. This is because these substances are exposed to oxidation reactions, which are catalysed by the polyphenol oxidase (PPO), leading to a decrease in the quality of the wines produced. PPO is inactivated by SO2, but currently, due to the restrictions of the legislation, other methodologies are being investigated. Ultra-High Pressure Homogenization (UHPH) is a non-thermal physic technology that exerts an ultrahigh pressure pumping (>200 MPa) of a fluid through a valve in a continuous system.

Nitrogen forms and Iron deficiency: how do Grapevine rootstocks responses change?

Grapevine rootstocks provide protection against environmental biotic and abiotic stresses. Nitrogen (N) and iron (Fe) are growth-limiting factors in many crop plants due to their effects on the chlorophyll and photosynthetic characteristics. Iron nutrition of plants can be significantly affected by different nitrogen forms through altering the uptake ratio of cations and anions, and changing rhizosphere pH. The aim of this study was to investigate the response mechanisms of grapevine rootstocks due to the interaction between different nitrogen forms and iron uptake.

A phylogenomic study reveals the major dissemination routes of ‘Tempranillo Tinto’ in the Iberian Peninsula

‘Tempranillo Tinto’ is a black-berried Iberian cultivar that originated from a hybridization between cvs. ‘Benedicto’ and ‘Albillo Mayor’ [1]. Today, it is the third most widely grown wine grape cultivar worldwide with more than 200,000 hectares of vineyards mostly distributed along the Iberian Peninsula, where it is also known as ‘Cencibel’, ‘Tinta de Toro’, ‘Tinta Roriz’, and ‘Aragonez’, among other synonyms. Here, we quantified the intra-varietal genomic diversity in this cultivar through the study of 35 clones or ancient vines from seven different Iberian wine-making regions. A comparative analysis after Illumina whole-genome sequencing revealed the presence of 1,120 clonal single nucleotide variants (SNVs).