terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Role of anthocyanins and copigmentation in flavonol solubility in red wines 

Role of anthocyanins and copigmentation in flavonol solubility in red wines 

Abstract

Over the last years, due to climate change, several red wines, such as the Sangiovese wines, have been often subjected to loss of clarity due to the formation of deposits of fine needle-shaped crystals. This phenomenon turned out to be due to an excess of quercetin (Q) and its glycosides (Q-Gs) in wines. These compounds are synthesized to a large extent when grapes are excessively exposed to UVB radiations in vineyards[1]. Unfortunately, it is not easy to predict the degree of Q precipitation because its solubility strongly depends on the wine and matrix composition[2].  Surprisingly, some red wines rich in anthocyanins, even if contained high amount of Q, did not show any precipitates. Likely anthocyanins favour the solubility of Q since flavonols are among the most powerful cofactors involved in the copigmentation phenomenon. Thus, in this study, the role of anthocyanins in Q solubility was evaluated by adding known amounts of grape-derived anthocyanins into model solutions containing either Q or Q-Gs. The effects of pH and time on the Q solubility, copigmentation as well as on the hydrolysis of Q-Gs were determined. Our data showed that the solubility of Q passed from 5 mg/L to 25 mg/L when the amount of grape-derived anthocyanins added into the model solutions increased from 0 to 500 mg/L. Experimental samples were investigated over 30 days and significant changes in Q solubility, correlated to the variation in copigmentation and in anthocyanins/quercetin ratio, were observed. These results can assist wine producers to better evaluate the level of flavonol precipitation in red wines and to develop appropriate strategies in order to avoid undesired precipitations in bottle.   

Acknowledgements: The authors would like to thank the Biolaffort Company for funding.

References:

1) Martínez-Lüscher J. et al. (2019). Flavonol profile is a reliable indicator to assess canopy architecture and the exposure of red wine grapes to solar radiation. Frontiers in plant science, 10, 10. DOI 10.3389/fpls.2019.00010.

2) Gambuti A. et al. (2020). New insights into the formation of precipitates of quercetin in Sangiovese wines. Journal of Food Science and Technology, 57, 2602-2611. DOI 10.1007/s13197-020-04296-7

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Alessandra Luciano1, Luigi Picariello1, Martino Forino1, Angelita Gambuti1*

1 Department of Agricultural Sciences, Section of Vine and Wine Sciences, University of Napoli ″Federico II″, Viale Italia, Avellino 83100, Italy

Contact the author*

Keywords

quercetin, quercetin glycosides, solubility, red wines, anthocyanins, copigmentation crystals, precipitation

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Polyphenol content of cork granulates at different steps of the manufacturing process of microagglomerated stoppers treated with supercritical CO2 used for wine bottling

The wine closure industry is mainly divided into three categories: screw caps, synthetic closures, and cork-based closures. Among this latter, microagglomerated cork stoppers treated with supercritical CO2 are now widely used, especially to avoid cork taint contaminations[1]. They are designed with cork granules obtained from cork offcuts of the punching process during the natural cork stoppers production. A previous study[2] showed that these stoppers released fewer polyphenols in 12 % (v/v) hydroalcoholic solution than natural cork stoppers.

Differences in metabolism among species and hybrids of the genus Saccharomyces during wine fermentation unveiled by multi-omic analysis 

Yeast species S. cerevisiae, S. uvarum, S. kudriavzevii and their hybrids present clear metabolic differences, even when we compared S. cerevisiae wine versus wild strain. These species and hybrids produced significantly higher amounts of glycerol, organic acids, 2,3-butanediol, and 2-phenyl ethanol and a reduction of the ethanol yield, properties very interesting in the sector to deal with climate change effects. To understand the existing differences, we have used several omics techniques to analyze the dynamics of the (intra- and extracellular) metabolomes and/or transcriptomes of representative strains of S. cerevisiae, S. uvarum, S. kudriavzevii, and hybrids.

Atypical aging and hydric stress: insights on an exceptionally dry year

Atypical aging (ATA) is a white wine fault characterized by the appearance of notes of wet rag, acacia blossoms and naphthalene, along with the vanishing of varietal aromas. 2-aminoacetophenone (AAP) – a degradation compound of indole-3-acetic acid (IAA) – is regarded as the main sensorial and chemical marker responsible for this defect. About the origin of ATA, a stress reaction occurring in the vineyard has been looked as the leading cause of this defect. Agronomic, climatic and pedological factors are the main triggers and among them, drought stress seems to play a crucial role.[1]

Application of an in vitro digestion model to study the bioaccessibility and the effect of the intestinal microbiota on the red wine proanthocyanidins 

Proanthocyanidins are important phenolic fraction for wine quality, contributing to astringency, bitterness and color. Their metabolism begins in the mouth and continues throughout the gastrointestinal tract; however, most of them are accumulated in the colon where are metabolized by the intestinal microbiota, giving rise to a whole series of phenolic acids that may have greater activity at physiological level than the precursors[1]. This study aimed to evaluate in vitro the bioaccessibility of proanthocyanidins in a red wine developed by Bodegas Pradorey, as well as to evaluate the potential effect of intestinal microbiota on polyphenols metabolism identifying and quantifying secondary metabolites.

Drought responses of grapevine cultivars under different environments

Using grapevine genetic diversity is one of the strategies to adapt viticulture to climate change. In this sense, assessing the plasticity of cultivars in their responses to environmental conditions is essential. For this purpose, the drought tolerance of Grenache, Tempranillo and Semillon cultivars grafted onto SO4 was evaluated at two experimental vineyards, one located in Valencia (Spain) and the other in Bordeaux (France). This was done by assessing gas exchange parameters, water relations and leaf hydraulic traits at the end of the season.