terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Role of anthocyanins and copigmentation in flavonol solubility in red wines 

Role of anthocyanins and copigmentation in flavonol solubility in red wines 

Abstract

Over the last years, due to climate change, several red wines, such as the Sangiovese wines, have been often subjected to loss of clarity due to the formation of deposits of fine needle-shaped crystals. This phenomenon turned out to be due to an excess of quercetin (Q) and its glycosides (Q-Gs) in wines. These compounds are synthesized to a large extent when grapes are excessively exposed to UVB radiations in vineyards[1]. Unfortunately, it is not easy to predict the degree of Q precipitation because its solubility strongly depends on the wine and matrix composition[2].  Surprisingly, some red wines rich in anthocyanins, even if contained high amount of Q, did not show any precipitates. Likely anthocyanins favour the solubility of Q since flavonols are among the most powerful cofactors involved in the copigmentation phenomenon. Thus, in this study, the role of anthocyanins in Q solubility was evaluated by adding known amounts of grape-derived anthocyanins into model solutions containing either Q or Q-Gs. The effects of pH and time on the Q solubility, copigmentation as well as on the hydrolysis of Q-Gs were determined. Our data showed that the solubility of Q passed from 5 mg/L to 25 mg/L when the amount of grape-derived anthocyanins added into the model solutions increased from 0 to 500 mg/L. Experimental samples were investigated over 30 days and significant changes in Q solubility, correlated to the variation in copigmentation and in anthocyanins/quercetin ratio, were observed. These results can assist wine producers to better evaluate the level of flavonol precipitation in red wines and to develop appropriate strategies in order to avoid undesired precipitations in bottle.   

Acknowledgements: The authors would like to thank the Biolaffort Company for funding.

References:

1) Martínez-Lüscher J. et al. (2019). Flavonol profile is a reliable indicator to assess canopy architecture and the exposure of red wine grapes to solar radiation. Frontiers in plant science, 10, 10. DOI 10.3389/fpls.2019.00010.

2) Gambuti A. et al. (2020). New insights into the formation of precipitates of quercetin in Sangiovese wines. Journal of Food Science and Technology, 57, 2602-2611. DOI 10.1007/s13197-020-04296-7

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Alessandra Luciano1, Luigi Picariello1, Martino Forino1, Angelita Gambuti1*

1 Department of Agricultural Sciences, Section of Vine and Wine Sciences, University of Napoli ″Federico II″, Viale Italia, Avellino 83100, Italy

Contact the author*

Keywords

quercetin, quercetin glycosides, solubility, red wines, anthocyanins, copigmentation crystals, precipitation

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Detoxification capacities of heavy metals and pesticides by yeasts 

Winegrowing is still characterized by the extensive use of chemical fertilizers and plant protection products, despite strong recommendations to limit these practices. A part of these xenobiotics and metals are then found in grape juice and wine, causing a major health concern, as well as negatively affecting the fermentation process. In recent years, there has been renewed interest in non-Saccharomyces yeasts. These species have a wide phenotypic diversity, which would be exploited to broaden the aromatic palette of wines.

The characterization of Vitis vinifera L cv. Cabernet sauvignon: the contribution of Ecklonia maxima seaweed extract

Biostimulants and biofertilizers are considered environmentally friendly and cost-effective alternatives to synthetic fertilizers, plant growth regulators and crop improvement products. Broadly, plant biostimulants are expected to improve nutrient use efficiency, tolerance to abiotic stress, quality traits and availability of nutrients in the soil or rhizosphere. Currently, seaweed extracts account for more than 33% of the total plant biostimulant market. Within this category, Ascophyllum nodosum (AN), is the most widely studied and applied in biostimulant formulations.

The effect of ozonated water treatment on the metabolic profile and resistance of vines to Downy and powdery mildew 

Ozone is a potent oxidizing compound that quickly decomposes into oxygen without residues. Previous works reported that ozone is not only a disinfectant that directly harms the pathogens of the vine but also activates systemic defense systems in the plant by activating oxidative stress. We assume these systemic defense mechanisms are essential to the vines’ resistance to downy and powdery mildew (Plasmopara viticola & Erysiphe necator, respectively). The goals of the research are to examine the effect of spraying with ozone water on the plant’s resistance against the mentioned pathogens as well as to characterize the metabolic profile of the plants treated with ozone as well as physiological characteristics in the vines such as the level of Photosynthesis and crop yield. Vines in the vineyard sprayed with ozone water at concentrations of 2 and 4 PPM weekly and biweekly, untreated control & conventional spray. Leaves were taken from vines 2,4,7,9 and 11 days after exposure to ozone and inoculated with the pathogens.

Effect of pH and ethanol on Lactiplantibacillus plantarum in red must fermentation: potential use of wine lees

Wine is the result of the alcoholic fermentation (AF) of grape must. Besides AF, wine can also undergo the malolactic fermentation (MLF) driven out by lactic acid bacteria (LAB). Among LAB, Oenococcus oeni and Lactiplantibacillus plantarum are the dominant species in wine. Even if O. oeni is the most common LAB undergoing MLF in wine, due to its high tolerance to wine conditions, L. plantarum can be used to undergo MLF in must. The moderate tolerance of L. plantarum to low pH and ethanol, may compromise the fermentative process in harsh wines.

The potential of some native varieties of Argentina for the production of sparkling wines. Effect of lees contact time 

Grapevine varieties from South-America, commonly known as criollas, originated because of the natural crossbreeding of grapevine varieties brought by the Spaniards. The objective of this work was to evaluate the potential of some varieties to produce sparkling wines considering the effect of lees contact time. The following varieties were used: Moscatel Rosado, Criolla Chica, Pedro Gimenez, Blanca Oval, Canelón, and the European variety Chardonnay (control), planted in the ampelographic collection of EEA Mendoza INTA (Argentina). Pilot-scale vinifications were carried out to obtain the base wines, in 20 L glass containers. The second fermentation was performed through the traditional method.