terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 A novel approach for the identification of new biomarkers of wine consumption in human urine using untargeted metabolomics

A novel approach for the identification of new biomarkers of wine consumption in human urine using untargeted metabolomics

Abstract

Wine is one of the most representative components of Mediterranean diet. Moderate wine intake together with food, has been positively correlated with reduced risk of many chronic diseases. This beneficial effect seems to be ascribed to elevated polyphenolic content of wine [1]. Traditional approaches for the identification of wine biomarkers consumption include targeted metabolomics that focuses on the quantification of well-defined metabolites, losing a valuable information about a massive number of compounds. On the other hand, untargeted metabolomics can disclose a large quantity of signals corresponding to potential biomarkers in a single analysis with high sensitivity and resolution. This work focuses on the identification of wine intake biomarkers in 24-h urine samples of free-living volunteers using untargeted metabolomics approach. Two groups were included: (i) volunteers with daily and moderate wine consumption, and (ii) control group, volunteers who never drink wine. Urine samples (24-h) were analysed by liquid chromatography coupled with high-resolution mass spectrometry (UPLC-QToF), using two stationary phases (RP and HILIC) to separate metabolites of different polarities, moreover all the analyses were done in both positive and negative ionization modes. The most significant compounds highlighted after performing an OPLS-DA were tentatively identified based on their accurate masses and spectra information. Different metabolites associated with wine intake have been tentatively proposed, such as aminoacids and peptides, and different phenol metabolites.

Acknowledgements: MCIN / AEI /10.13039/501100011033 and the European Union NextGenerationEU/PRTR through the project PID2019-108851RB-C22. M.J-S. thanks University of La Rioja for her PostDoc grant financed by the European Union-NextGenerationEU.

References:

  1. Hrelia S. et al. (2023) Moderate Wine Consumption and Health: A Narrative Review. Nutrients, 15: 175-200, DOI 10.3390/nu15010175.

DOI:

Publication date: October 5, 2023

Issue: ICGWS 2023

Type: Article

Authors

Jiménez-Salcedo M.1,2*, Manzano JI.1, Pérez-Matute, P.3, Motilva MJ. 1

1 Instituto de Ciencias de la Vid y el Vino-ICVV (CSIC, UR, GR) 26007 Logroño (España)
2 Universidad de La Rioja, 26006 Logroño (España)
3 Infectious Diseases, Microbiota and Metabolism Unit, Center for Biomedical Research of La Rioja (CIBIR), CSIC Associated Unit. 26006 Logroño (España)

Contact the author*

Keywords

untargeted metabolomics, wine, biomarker, polyphenols

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Agronomic behavior of three grape varieties in different planting density and irrigation treatments

In the O Ribeiro Denomination of Origin, there is a winemaking tradition of growing vines under a high-density plantation framework (8,920 vines/ha) and maintaining its vegetative cycle under rainfed conditions.
Currently, viticulture is advancing to plantation frames in which the density is considered medium (5,555 vines/ha), thus allowing mechanized work to be carried out for vineyard management operations. Although, the application of irrigation applied proportionally to the needs of the vegetative cycle of the vine, is a factor that increasingly helps a good development of the vine compared to the summer period, with increasingly uncertain weather forecasts.

Comparison of the effects of hormone- and natural-based elicitors on key metabolic pathways in cv. Tempranillo

One of the most important effects of climate change in wine-growing areas is the advance of phenological stages, especially concerning early berry ripening. In the hottest seasons, this results in a lack of synchrony between sugar and phenolic ripeness. In order to cope with this fact, a general effort is being made by researchers and growers aiming at delaying ripening through different strategies. One of the proposed approaches is the application of elicitors. This study aims to assess the effect at the transcriptomic level of application of three elicitors (Vitalfit, Fruitel, and Protone) in Tempranillo.

Adsorption of tetraconazole by organic residues and vineyard organically-amended soils 

Spain is the country with the largest wine-producing area in the EU and its productivity is largely controlled applying fungicides. However, residues of these compounds can move and contaminate surface and groundwater. The objective of this work was to evaluate the capacity of bioadsorbents from different origin to adsorb and immobilize tetraconazole by themselves or when applied as organic soil amendment, and to prevent soil and water contamination by this fungicide. The adsorption of tetraconazole by 3 organic residues: spent mushroom substrate (SMS), green compost (GC) and vine pruning sawdust (VP), as well as by vineyard soils unamended and amended individually with these residues at 1.5% (w/w) was evaluated using the batch equilibrium technique.

Influence of polysaccharide extracts from wine by-products on the volatile composition of sparkling white wines

In the production of sparkling wines, during the second fermentation, mannoproteins are released by yeast autolysis, which affect the quality of the wines. The effect of mannoproteins has been extensively studied, and may affect aroma and foam quality. However, there are no studies on the effect of other polysaccharides such as those from grapes. Considering the large production of waste from the wine industry, it was proposed to obtain polysaccharide-rich extracts from some of these by-products[1].

Late winter pruning induces a maturity delay under temperature-increased conditions in cv. Merlot from Chile

Chile is considered vulnerable to climate change; and these phenomena affect several mechanisms in the grape physiology and quality. The global temperature increase affects sugar contents, organic acids, and phenolic compounds in grapes, producing an imbalance maturity. In this sense, an alternative to reduce the impact is to perform pruning after vine budburst, known as “Late Pruning” (LP).