terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Effect of drought on grapevine wood fungal pathogen communities using a metatranscriptomics approach

Effect of drought on grapevine wood fungal pathogen communities using a metatranscriptomics approach

Abstract

Crops are facing increasing biotic and abiotic stress pressures due to global changes. However, trade-off mechanisms between these stresses and the underlying physiological processes are still poorly understood, especially in perennial crop species. To better understand these trade-offs, we studied the effect of drought on grapevine (Vitis vinifera) physiology and esca-related wood fungal communities. Esca is a vascular disease caused by a community of wood-infecting pathogenic fungi, and characterized by trunk necrosis, leaf scorch symptoms, yield losses, and mortality. This grapevine disease lead to xylem hydraulic failure and leaf symptoms are inhibited by severe drought. To characterize the molecular processes underlying the interactions between drought and esca, we conducted two experiments on 30-year-old Sauvignon blanc vines, expressing or not esca leaf symptoms, and subjected or not to drought stress under controlled conditions. Sapwood samples from the trunks were used to perform community-level transcriptomics analyses. Results will be also analyzed in the light of others metabolomics and ecophysiological data acquired on wood and leaf samples. Such an integrative approach will provide new insights into the understanding of grapevine/esca pathosystem under drought conditions, in terms of physiological and functional responses in either host and pathogens.

Acknowledgements: The authors thanks Université de Bordeaux for funding the GPR (Great Research Project) Bordeaux Plant Science.

DOI:

Publication date: October 6, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Marie Chambard1,2, Ninon Dell’Acqua1, Giovanni Bortolami1, Dario Cantù3, Nathalie Ferrer1, Gregory A. Gambetta4, Marie Foulongne-Oriol2, Chloé E. L. Delmas1

1 INRAE Bordeaux Nouvelle Aquitaine, UMR 1065 SAVE, 71 avenue Edouard Bourlaux – CS 20032, 33882 Villenave d’Ornon cedex
2 INRAE Bordeaux Nouvelle Aquitaine, UR 1264 MycSA, 71 avenue Edouard Bourlaux – CS 20032, 33882 Villenave d’Ornon cedex

3 Department of Viticulture and Enology, University of California, Davis, One Shields Ave, Davis, CA 95618, USA
4 INRAE Bordeaux Nouvelle Aquitaine, UMR 1287 EGFV, 71 avenue Edouard Bourlaux – CS 20032, 33882 Villenave d’Ornon cedex

Contact the author*

Keywords

Vitis vinifera Sauvignon Blanc, esca disease, drought, metatranscriptomics, physiology

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Combined use of leaf removal and natural shading to delay grape ripening in Manto negro (Vitis vinifera L.) under deficit irrigation 

The increasingly frequent heat waves during grape ripening pose challenges for premium wine grape production. This makes the development of irrigation and canopy management techniques of great importance to maximize yield and grape quality. A field experiment was carried out during 2021 and 2022 using Manto negro wine grapes to study the effect of two irrigation strategies and different light exposure levels on grape quality.

The potential of some native varieties of Argentina for the production of sparkling wines. Effect of lees contact time 

Grapevine varieties from South-America, commonly known as criollas, originated because of the natural crossbreeding of grapevine varieties brought by the Spaniards. The objective of this work was to evaluate the potential of some varieties to produce sparkling wines considering the effect of lees contact time. The following varieties were used: Moscatel Rosado, Criolla Chica, Pedro Gimenez, Blanca Oval, Canelón, and the European variety Chardonnay (control), planted in the ampelographic collection of EEA Mendoza INTA (Argentina). Pilot-scale vinifications were carried out to obtain the base wines, in 20 L glass containers. The second fermentation was performed through the traditional method.

Wine odors: chemicals, physicochemical and perceptive processes involved in their perception

The odors of wines are diverse, complex and dynamic and much research has been devoted to the understanding of their chemical bases. However, while the “basic” chemical part of the problem, namely the identity of the chemicals responsible for the different odor nuances, was satisfactorily solved years ago, there are some relevant questions precluding a clear understanding. These questions are related to the physicochemical interactions determining the effective volatilities of the odorants and, particularly, to the perceptual interactions between different odor molecules affecting in different ways to the final sensory outputs.

Vineyard yield estimation using image analysis: assessing bunch occlusions and its dependency on fruiting zone canopy features

Performing accurate vineyard yield estimation is of upmost importance as it provides important benefits to the whole vine and wine industry. Recently, image-analysis approaches have been explored to address this issue however this approach has as main challenge the bunch occlusion, mostly by vegetation but also by neighboring bunches. The present work aims at assessing the magnitude of bunch occlusion by neighboring bunches and to evaluate its dependency on a selection of vegetative and reproductive vine parameters assessed at fruiting zone. Forty vine segments (1 m) of two vineyard plots of the white cultivars ‘Alvarinho’ and ‘Arinto’ were assessed for vegetative and reproductive features at fruiting zone and imaged with a 2D camera.

“Compost Application in the Vineyard: Effects on Soil Nutrition and Compaction”

The mechanization of pruning and harvesting in vineyards has increased the risk of soil compaction. To reclaim soil properties or avoid this degradation process, it is crucial to properly manage the soil organic matter, and the application of compost derived from the vines themselves is a strategy to achieve this. The objective of this study was to evaluate the properties of soil treated with different doses of compost applied both on the vine row and the inter rows of a Vitis vinifera crop.