terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Effect of drought on grapevine wood fungal pathogen communities using a metatranscriptomics approach

Effect of drought on grapevine wood fungal pathogen communities using a metatranscriptomics approach

Abstract

Crops are facing increasing biotic and abiotic stress pressures due to global changes. However, trade-off mechanisms between these stresses and the underlying physiological processes are still poorly understood, especially in perennial crop species. To better understand these trade-offs, we studied the effect of drought on grapevine (Vitis vinifera) physiology and esca-related wood fungal communities. Esca is a vascular disease caused by a community of wood-infecting pathogenic fungi, and characterized by trunk necrosis, leaf scorch symptoms, yield losses, and mortality. This grapevine disease lead to xylem hydraulic failure and leaf symptoms are inhibited by severe drought. To characterize the molecular processes underlying the interactions between drought and esca, we conducted two experiments on 30-year-old Sauvignon blanc vines, expressing or not esca leaf symptoms, and subjected or not to drought stress under controlled conditions. Sapwood samples from the trunks were used to perform community-level transcriptomics analyses. Results will be also analyzed in the light of others metabolomics and ecophysiological data acquired on wood and leaf samples. Such an integrative approach will provide new insights into the understanding of grapevine/esca pathosystem under drought conditions, in terms of physiological and functional responses in either host and pathogens.

Acknowledgements: The authors thanks Université de Bordeaux for funding the GPR (Great Research Project) Bordeaux Plant Science.

DOI:

Publication date: October 6, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Marie Chambard1,2, Ninon Dell’Acqua1, Giovanni Bortolami1, Dario Cantù3, Nathalie Ferrer1, Gregory A. Gambetta4, Marie Foulongne-Oriol2, Chloé E. L. Delmas1

1 INRAE Bordeaux Nouvelle Aquitaine, UMR 1065 SAVE, 71 avenue Edouard Bourlaux – CS 20032, 33882 Villenave d’Ornon cedex
2 INRAE Bordeaux Nouvelle Aquitaine, UR 1264 MycSA, 71 avenue Edouard Bourlaux – CS 20032, 33882 Villenave d’Ornon cedex

3 Department of Viticulture and Enology, University of California, Davis, One Shields Ave, Davis, CA 95618, USA
4 INRAE Bordeaux Nouvelle Aquitaine, UMR 1287 EGFV, 71 avenue Edouard Bourlaux – CS 20032, 33882 Villenave d’Ornon cedex

Contact the author*

Keywords

Vitis vinifera Sauvignon Blanc, esca disease, drought, metatranscriptomics, physiology

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Green pruning of shoots to force new sprouting of buds, in fruit set and in pea size: vegetative, productive and maturation effects, in cv. Verdejo

The context of climate crisis leads to the acceleration of technological ripening of grapes, with unsuitable loss of acidity, so various vineyard management alternatives are being considered to delay the grape ripening. The delay of the vegetative cycle towards a period of milder temperatures affects ripening, but vine behavior can vary according to the area, conduction, watering, variety, etc. A work is proposed to know the response to the green pruning of shoots, executed in fruit set and in pea size, in cv. Verdejo.

Influence of different Lachancea thermotolerans strains in wine acidity

Wine acidity is a parameter of great importance that influences different quality factors of the product such as biological stability or organoleptic characteristics. In the current context of climate change, which gives rise to wines with higher levels of ethanol and lower acidity, the biological acidification with yeast species such as Lachancea thermotolerans could be a solution.
In this work, the effect of the inoculation of different L. thermotolerans on the acidity of wine was studied.

Agronomic and oenological behavior of the minority Mandón variety on two rootstocks in the D.O. Arribes

A large population of vines of the Mandón minority red variety (synonymous with Garró) has been located in old vineyards of the D.O. Arribes (Zamora and Salamanca) to conserve and recover this minority variety. The wines made with this variety are characterized by their good structure and color, interesting harmony, an excellently low pH, with high acidity, as well as complex aromas of blue fruits and a marked and expressive minerality.

Grape pomace, an active ingredient at the intestinal level: Updated evidence

Grape pomace (GP) is a winemaking by-product particularly rich in (poly)phenols and dietary fiber, which are the main active compounds responsible for its health-promoting effects. GP-derived products have been proposed to manage cardiovascular risk factors, including endothelial dysfunction, inflammation, hypertension, hyperglycemia, and obesity. Studies on the potential impact of GP on gut health are much more recent. However, it is suggested that, to some extent, this activity of GP as a cardiometabolic health-promoting ingredient would begin in the gastrointestinal tract as GP components (i.e., (poly)phenols and fiber) undergo extensive catabolism, mainly by the action of the intestinal microbiota, that gives rise to low-molecular-weight bioactive compounds that can be absorbed and utilized by the body.

Conventional and alternative pest management strategies: a comparative proteomic study on musts

In a context of sustainable agriculture, “agroecological immunity” is an emerging concept to reduce the use of chemical pesticides to protect crops against pathogens. This alternative strategy aims to combine different levers including the use of “bio”solutions. These include biocontrol products, some of which being plant defense elicitors, as well as products authorized in organic farming such as copper or sulfur. In vineyards, depending on climate conditions, powdery and downy mildews can be devastating diseases.