terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Analysis of volatile composition of interaction between the pathogen E. necator and two grapevine varieties

Analysis of volatile composition of interaction between the pathogen E. necator and two grapevine varieties

Abstract

Volatile organic compounds (VOCs) are emitted by nearly all plant organs of the plants, including leaves. They play a key role in the communication with other organisms, therefore they are involved in plant defence against phytopathogens. In this study VOCs from grapevine leaves of two varieties of Vitis vinifera infected by Erysiphe necator were analysed. The varieties were selected based on their susceptibility to pathogen, Kishmish Vatkana has the Ren1 resistance gene and Zamarrica showed high susceptibility in previous trials. Three plants per variety were inoculated with a conidial solution (>105 conidias/ml). Leaves were extracted by headspace solid phase microextraction and analysed by GC-MS at 1 day post inoculation. More than 70 compounds were annotated, leaf extracts of Kishmish were richer in compounds than Zamarrica. In respond to pathogen, the accumulation of aldehydes, mainly 2-pentenal, and ethyl esters in Kismish were scored. Zamarrica had a greater number of compounds that modified their concentrations by the interaction. When compared between treated leaves of both varieties, differences in terpenes and aldehydes were found. These results show that VOCs may play an important role in the response to pathogen attack, but further transcriptomic analyses are required to know the possible effects of VOCs on defence system, mainly in the activation of resistance mechanisms.

Acknowledgements: Financial support from Ministerio de Ciencia Innovación y Universidades RTI2018-101085-R-C32.

DOI:

Publication date: October 6, 2023

Issue: ICGWS 2023

Type: Poster

Authors

María M. Hernández1*, Carolina Castillo Rio2, Sara Isabel Blanco González2, Cristina M. Menéndez1

1 Instituto de Ciencias de la Vid y el Vino (ICVV), Finca La Grajera, Carretera de Burgos km 6, 26007, Logroño
2 Dpto. de Agricultura y Alimentación, Universidad de La Rioja, C/Madre de Dios 51, 26006, Logroño

Contact the author*

Keywords

powdery mildew, plant defence, interaction plant – pathogen, secondary metabolites

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Genetic variation among wild grapes native to Japan

Domesticated grapes are assumed to have originated in the Middle East. However, a considerable number of species are native in East Asian countries such as China, Korea and Japan as well. Evidence suggests that a total of seven species and eight varieties have been found to be native to Japan. A wide level variation in morphology, genetic and fruit composition exist in wild grape native to Japan.

Polyphenol content of cork granulates at different steps of the manufacturing process of microagglomerated stoppers treated with supercritical CO2 used for wine bottling

The wine closure industry is mainly divided into three categories: screw caps, synthetic closures, and cork-based closures. Among this latter, microagglomerated cork stoppers treated with supercritical CO2 are now widely used, especially to avoid cork taint contaminations[1]. They are designed with cork granules obtained from cork offcuts of the punching process during the natural cork stoppers production. A previous study[2] showed that these stoppers released fewer polyphenols in 12 % (v/v) hydroalcoholic solution than natural cork stoppers.

Sugar accumulation disorder Berry Shrivel – from current knowledge towards novel hypothesis

In contrast to fruit and grape berry ripening, the biological processes causing ripening disorders are often much less understood, although shriveling disorders of fruits are manifold and contribute to yield losses and reduced fruit quality worldwide. Shrinking berries are a common feature for all shriveling disorders in grapevine although their timing of appearance during the berry ripening process and their underlying induction processes distinct them from each other. The sugar accumulation disorder Berry Shrivel (BS) is characterized by a suppression of sugar accumulation short after veraison resulting in berries low in sugar content and anthocyanins in berry skins, while the organic acid content is similar. Recent studies analyzed the biochemical, morphological and molecular processes affected in BS berries and linked early changes to the period of ripening onset [1,2].

Effect of foliar application of urea and nano-urea on the cell wall of Monastrell grape skins

The foliar application of urea has been shown to be able to satisfy the specific nutritional needs of the vine as well as to increase the nitrogen composition of the must. On the other hand, the use of nanotechnology could be of great interest in viticulture as it would help to slow down the release of urea and protect it against possible degradation. Several studies indicate that cell wall synthesis and remodeling are affected by nitrogen availability.

Development of a new method for detecting acetic acid bacteria in wine

The presence of acetic acid bacteria in wine can lead to the appearance of acetic acid at concentrations above the perception threshold, causing the wine rejection by the consumer. During the winemaking process, avoiding the presence of acetic acid bacteria is very difficult, as there is always a residual population accompanying the wine[1], and the problem arises with the significant development of these microorganisms that metabolizes large amounts of acetic acid.
The concern of wineries to control the presence of acetic acid bacteria in wines during their conservation is due to the absence of simple and effective analyses that allow the detection of these microorganisms in the initial stages.