terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Analysis of volatile composition of interaction between the pathogen E. necator and two grapevine varieties

Analysis of volatile composition of interaction between the pathogen E. necator and two grapevine varieties

Abstract

Volatile organic compounds (VOCs) are emitted by nearly all plant organs of the plants, including leaves. They play a key role in the communication with other organisms, therefore they are involved in plant defence against phytopathogens. In this study VOCs from grapevine leaves of two varieties of Vitis vinifera infected by Erysiphe necator were analysed. The varieties were selected based on their susceptibility to pathogen, Kishmish Vatkana has the Ren1 resistance gene and Zamarrica showed high susceptibility in previous trials. Three plants per variety were inoculated with a conidial solution (>105 conidias/ml). Leaves were extracted by headspace solid phase microextraction and analysed by GC-MS at 1 day post inoculation. More than 70 compounds were annotated, leaf extracts of Kishmish were richer in compounds than Zamarrica. In respond to pathogen, the accumulation of aldehydes, mainly 2-pentenal, and ethyl esters in Kismish were scored. Zamarrica had a greater number of compounds that modified their concentrations by the interaction. When compared between treated leaves of both varieties, differences in terpenes and aldehydes were found. These results show that VOCs may play an important role in the response to pathogen attack, but further transcriptomic analyses are required to know the possible effects of VOCs on defence system, mainly in the activation of resistance mechanisms.

Acknowledgements: Financial support from Ministerio de Ciencia Innovación y Universidades RTI2018-101085-R-C32.

DOI:

Publication date: October 6, 2023

Issue: ICGWS 2023

Type: Poster

Authors

María M. Hernández1*, Carolina Castillo Rio2, Sara Isabel Blanco González2, Cristina M. Menéndez1

1 Instituto de Ciencias de la Vid y el Vino (ICVV), Finca La Grajera, Carretera de Burgos km 6, 26007, Logroño
2 Dpto. de Agricultura y Alimentación, Universidad de La Rioja, C/Madre de Dios 51, 26006, Logroño

Contact the author*

Keywords

powdery mildew, plant defence, interaction plant – pathogen, secondary metabolites

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Effect of two water deficit regimes on the agronomic response of 12 grapevine varieties cultivated in a semi-arid climate

The Mediterranean basin is one of the most vulnerable regions to Climate Change effects. According to unanimous forecasts, the vineyards of Castilla-La Mancha will be among the most adversely affected by rising temperatures and water scarcity during the vine’s vegetative period. One potential strategy to mitigate the negative impacts of these changes involves the identification of grapevine varieties with superior water use efficiency, while ensuring satisfactory yields and grape quality.

Application of antagonistic Metschnikowia strains against Botrytis cinerea in vineyards 

Less and less chemical plant protection products are approved by the E U. Plant pathogenic fungi become increasingly resistant to the active ingredients that have been around for a long time. Besides, there is a valid demand for effective products that can be applied in organic cultivation.
We examined Metschnikowia strains under laboratory conditions in order to find effective strains against B. cinerea. The antimicrobial mechanism of these yeasts is based on the competition for the ferric ions from the environment. Metschnikowia cells release the pulcherriminic acid which chelates with Fe3+, forming the pigment pulcherrimin.

Volatile composition of Cabernet Sauvignon wines from Argentina, Portugal and Spain

Cabernet Sauvignon is one of the most cultivated grape varieties worldwide being grown in different environmental conditions due to its excellent adaptability. Volatile compounds deeply contribute to the sensory properties of wines therefore to wine quality. The aim of this work was to compare the aroma profile of Cabernet Sauvignon wines from different geographical areas and climatic conditions, namely from Argentina, Portugal and Spain, from the vintage 2022. In addition, the volatile composition of the Cabernet Sauvignon Portuguese wines from three vintages was evaluated.

Development of a new method for detecting acetic acid bacteria in wine

The presence of acetic acid bacteria in wine can lead to the appearance of acetic acid at concentrations above the perception threshold, causing the wine rejection by the consumer. During the winemaking process, avoiding the presence of acetic acid bacteria is very difficult, as there is always a residual population accompanying the wine[1], and the problem arises with the significant development of these microorganisms that metabolizes large amounts of acetic acid.
The concern of wineries to control the presence of acetic acid bacteria in wines during their conservation is due to the absence of simple and effective analyses that allow the detection of these microorganisms in the initial stages.

White grape must processed by UHPH as an alternative to SO2 addition: Effect on the phenolic composition in three varieties

The quantity and distribution of polyphenols in musts play a fundamental role in the white winemaking. This is because these substances are exposed to oxidation reactions, which are catalysed by the polyphenol oxidase (PPO), leading to a decrease in the quality of the wines produced. PPO is inactivated by SO2, but currently, due to the restrictions of the legislation, other methodologies are being investigated. Ultra-High Pressure Homogenization (UHPH) is a non-thermal physic technology that exerts an ultrahigh pressure pumping (>200 MPa) of a fluid through a valve in a continuous system.