terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Application of antagonistic Metschnikowia strains against Botrytis cinerea in vineyards 

Application of antagonistic Metschnikowia strains against Botrytis cinerea in vineyards 

Abstract

Less and less chemical plant protection products are approved by the E U. Plant pathogenic fungi become increasingly resistant to the active ingredients that have been around for a long time. Besides, there is a valid demand for effective products that can be applied in organic cultivation.

We examined Metschnikowia strains under laboratory conditions in order to find effective strains against B. cinerea. The antimicrobial mechanism of these yeasts is based on the competition for the ferric ions from the environment. Metschnikowia cells release the pulcherriminic acid which chelates with Fe3+, forming the pigment pulcherrimin. The ability of M. pulcherrima of depleting Fe from the growth media is correlated with it’s ability to inhibit the growth of many microorganism. We compared the strains’ ability to produce pulcherrimin and their ability to inhibit different pathogenic fungi in laboratory conditions. Pulcherrimin production was also reported in the case of other yeast species. Krause et al. (2018) described four genes (PUL1-4) forming a PULcherrimin (PUL) gene cluster present in Kluyveromyces. lactis, K. aestuarii, M. fructicola and Zygotorulaspora mraki. We have also identified the four PULgenes in our effective M. pulcherrima strains.

We conducted field studies in three vintages using a specific M. pulcherrima strains. Based on our results, we can state that we have found an effective method to protect grape against B cinerea, which can be applied both in organic cultivation and also before harvest in conventional technology.

Acknowledgements: The authors would like to thank Anita Kovács, Ilona Szilágyi and Szilvia Struba for the profession technical assistance. This research was financed by the grant 2020-1.1.2-PIACI-KFI-2020-00130

References:

1) Krause D.J., Kominek J., Opulente D.A., Shen X.X., Zhou X., Langdon Q.K., DeVirgiliof J., Hulfachora A.B., Kurtzmanf C.P., Hittinger C.T. 2018. Functional and evolutionary characterization of a secondary metabolite gene cluster in budding yeasts. Proc. Natl. Acad. Sci. 115, 11030-11035. DOI: 10.1073/pnas. 1806268115

DOI:

Publication date: October 6, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Zoltán Kállai1*, Kinga Czentye1, Matthias Sipiczki1

1Department of Genetics and Applied Microbiology, Faculty of Science and Technology, University of Debrecen, H-4032 Debrecen, Hungary

Contact the author*

Keywords

crop protection, bioprotection, antagonism, Metschnikowia, Botrytis cinerea 

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Adsorption of tetraconazole by organic residues and vineyard organically-amended soils 

Spain is the country with the largest wine-producing area in the EU and its productivity is largely controlled applying fungicides. However, residues of these compounds can move and contaminate surface and groundwater. The objective of this work was to evaluate the capacity of bioadsorbents from different origin to adsorb and immobilize tetraconazole by themselves or when applied as organic soil amendment, and to prevent soil and water contamination by this fungicide. The adsorption of tetraconazole by 3 organic residues: spent mushroom substrate (SMS), green compost (GC) and vine pruning sawdust (VP), as well as by vineyard soils unamended and amended individually with these residues at 1.5% (w/w) was evaluated using the batch equilibrium technique.

Selecting green cover species in the under-trellis zone of Lower Austrian vineyards

The under-trellis zone of vineyards is a sensitive area through which vines cover a significant portion of their nutrient and water needs. Mechanical and chemical methods are applied to suppress competing and tall-growing weeds to ensure optimal vine growth conditions. In addition to higher operating costs and depending on the soil conditions, these practices might lead to a long-term reduction in soil fertility and biodiversity. The presented study aims to analyse the suitability and interspecies competition of a selected green cover mixture of five local herbaceous species as potential green cover mixture in the under-trellis area of Lower Austrian vineyards.

Grape pomace, an active ingredient at the intestinal level: Updated evidence

Grape pomace (GP) is a winemaking by-product particularly rich in (poly)phenols and dietary fiber, which are the main active compounds responsible for its health-promoting effects. GP-derived products have been proposed to manage cardiovascular risk factors, including endothelial dysfunction, inflammation, hypertension, hyperglycemia, and obesity. Studies on the potential impact of GP on gut health are much more recent. However, it is suggested that, to some extent, this activity of GP as a cardiometabolic health-promoting ingredient would begin in the gastrointestinal tract as GP components (i.e., (poly)phenols and fiber) undergo extensive catabolism, mainly by the action of the intestinal microbiota, that gives rise to low-molecular-weight bioactive compounds that can be absorbed and utilized by the body.

Reconstructing ancient microbial fermentation genomes from the wine residues of Herod, Roman king of Judea

The fortress of the Herodium, built towards the end of the first century BCE/ante Cristo, on the orders of Herod the Great, Roman client king of Judea, attests the expansion of Roman influence in the eastern Mediterranean. During archaeological excavations of the Herodium in 2017[1], a winery was discovered on the ground floor of the palace, with an assortment of clay vessels in situ, including large dolia – clay fermentation vessels each capable of fermenting up to 300-400 L of wine. Thanks to the recent progresses in the field of paleogenomics[2], we could analyse the organic material consistent with grape pomace at the bottom of these vessels, by extracting and sequencing the DNA using shotgun metagenomics and targeted capture, aiming for enrichment of DNA from fermentation associated microbes.

Molecularly imprinted polymers: an innovative strategy for harvesting polyphenoles from grape seed extracts

Multiple sclerosis (MS) is a multifactorial autoimmune disease associating demyelination and axonal degeneration developing in young adults and affecting 2–3 million people worldwide. Plant polyphenols endowed with many therapeutic benefits associated with anti-inflammatory and antioxidant properties represent highly interesting new potential therapeutic strategies. We recently showed the safety and high efficiency of grape seed extract (GSE), a complex mixture of polyphenolics compounds comprising notably flavonoids and proanthocyanidins, in an experimental autoimmune encephalomyelitis (EAE) mouse model of MS.