terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Application of antagonistic Metschnikowia strains against Botrytis cinerea in vineyards 

Application of antagonistic Metschnikowia strains against Botrytis cinerea in vineyards 

Abstract

Less and less chemical plant protection products are approved by the E U. Plant pathogenic fungi become increasingly resistant to the active ingredients that have been around for a long time. Besides, there is a valid demand for effective products that can be applied in organic cultivation.

We examined Metschnikowia strains under laboratory conditions in order to find effective strains against B. cinerea. The antimicrobial mechanism of these yeasts is based on the competition for the ferric ions from the environment. Metschnikowia cells release the pulcherriminic acid which chelates with Fe3+, forming the pigment pulcherrimin. The ability of M. pulcherrima of depleting Fe from the growth media is correlated with it’s ability to inhibit the growth of many microorganism. We compared the strains’ ability to produce pulcherrimin and their ability to inhibit different pathogenic fungi in laboratory conditions. Pulcherrimin production was also reported in the case of other yeast species. Krause et al. (2018) described four genes (PUL1-4) forming a PULcherrimin (PUL) gene cluster present in Kluyveromyces. lactis, K. aestuarii, M. fructicola and Zygotorulaspora mraki. We have also identified the four PULgenes in our effective M. pulcherrima strains.

We conducted field studies in three vintages using a specific M. pulcherrima strains. Based on our results, we can state that we have found an effective method to protect grape against B cinerea, which can be applied both in organic cultivation and also before harvest in conventional technology.

Acknowledgements: The authors would like to thank Anita Kovács, Ilona Szilágyi and Szilvia Struba for the profession technical assistance. This research was financed by the grant 2020-1.1.2-PIACI-KFI-2020-00130

References:

1) Krause D.J., Kominek J., Opulente D.A., Shen X.X., Zhou X., Langdon Q.K., DeVirgiliof J., Hulfachora A.B., Kurtzmanf C.P., Hittinger C.T. 2018. Functional and evolutionary characterization of a secondary metabolite gene cluster in budding yeasts. Proc. Natl. Acad. Sci. 115, 11030-11035. DOI: 10.1073/pnas. 1806268115

DOI:

Publication date: October 6, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Zoltán Kállai1*, Kinga Czentye1, Matthias Sipiczki1

1Department of Genetics and Applied Microbiology, Faculty of Science and Technology, University of Debrecen, H-4032 Debrecen, Hungary

Contact the author*

Keywords

crop protection, bioprotection, antagonism, Metschnikowia, Botrytis cinerea 

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Accumulation of deleterious mutations in grapevine and its relationship with traits of interest for wine production and resilience

Deleterious mutations that severely reduce population fitness are rapidly removed from the gene pool by purifying selection. However, evolutionary drivers such as genetic drift brought about by demographic bottlenecks may comprise its efficacy by allowing deleterious mutations to accumulate, thereby limiting the adaptive potential of populations. Moreover, positive selection can hitchhike mildly deleterious mutations due to linkage caused by lack of recombination. Similarly, in the context of species domestication, artificial selection mimics these evolutionary processes, which can have undesirable consequences for production and resilience. In this study, we evaluated the extent of the accumulation of deleterious mutations and the magnitude of their effects (also known as genetic load) at the whole-genome scale for ca.

Identification of several glycosidic aroma precursors in six varieties of winemaking grapes and assessment of their aroma potential by acid hydrolysis

In winemaking grapes, it is known that most aroma compounds are present as non-volatile precursors, such as glycosidic precursors. In fact, there is strong evidence supporting the connection between the content of aroma precursors and the aromatic quality of wine [1]. Acid hydrolysis is preferred to reveal the aroma potential of winemaking grapes, as it predicts more accurately the chemical rearrangements occurring during fermentation in acidic environments [2]. In this study, a method involving a fast fermentation followed by acid hydrolysis at 75ºC was used to evaluate the accumulation of aroma compounds over time in fractions obtained from six different varieties of winemaking grapes.

Grapevine adaptation to drought and resistance to Neofusicoccum parvum, causal agent of Botryosphaeria dieback

The sustainability of viticulture in response to climate change has been addressed mainly considering agronomic impacts, such as water management and diseases, either separately or together.
In grapevines, there is strong evidence that different genotypes respond differently to biotic and abiotic stresses. A screening was conducted on various local cultivars in response to drought and Neofusicoum parvum infection aiming to evaluate their susceptibility to abiotic stress and resistance to fungal diseases.

Exploring the prevalence of esca-induced leaf symptoms in French vineyards and the role of climate: a national scale analysis

Esca, a severe trunk disease affecting vineyards, is caused by fungal pathogens that induce wood necrosis and decay, leaf symptoms, yield losses, and potentially a rapid death of the vine. The prevalence of this disease varies across years, regions, cultivars, and plot ages. Despite its significance in understanding and predicting dieback risk in different vineyards, the role of climate in trunk diseases remains a relatively unexplored research area. While some studies have demonstrated the impact of certain climatic conditions on the prevalence of the disease, they often focus on a limited number of plots and yield conflicting results.We conducted a statistical analysis, using a Bayesian approach on a national database comprising prevalence data of esca from over 500 different plots in France, spanning the years 2003 to 2022 and encompassing various cultivars.

Chemical and microbiological evaluation of Ribeiro wines (NW Spain)

Wine produced under Designation of Origin (DOP) Ribeiro, the oldest DOP in Galicia (NW Spain), are elaborated using local grape cultivars, grown at the valleys of Miño, Avia and Arnoia rivers. The landscape formed by slopes and terraces and the peculiar climate of continental character, softened by the proximity of Atlantic Ocean, make it an area of excellent aptitude for vine cultivation. In addition, small-scale farming and the use of traditional techniques for vineyard management provide a great diversity to Ribeiro wines. This study presents the evaluation of red and white wines (bottled or bulk wines) from DOP Ribeiro, produced between years 2018-2022.