terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Application of antagonistic Metschnikowia strains against Botrytis cinerea in vineyards 

Application of antagonistic Metschnikowia strains against Botrytis cinerea in vineyards 

Abstract

Less and less chemical plant protection products are approved by the E U. Plant pathogenic fungi become increasingly resistant to the active ingredients that have been around for a long time. Besides, there is a valid demand for effective products that can be applied in organic cultivation.

We examined Metschnikowia strains under laboratory conditions in order to find effective strains against B. cinerea. The antimicrobial mechanism of these yeasts is based on the competition for the ferric ions from the environment. Metschnikowia cells release the pulcherriminic acid which chelates with Fe3+, forming the pigment pulcherrimin. The ability of M. pulcherrima of depleting Fe from the growth media is correlated with it’s ability to inhibit the growth of many microorganism. We compared the strains’ ability to produce pulcherrimin and their ability to inhibit different pathogenic fungi in laboratory conditions. Pulcherrimin production was also reported in the case of other yeast species. Krause et al. (2018) described four genes (PUL1-4) forming a PULcherrimin (PUL) gene cluster present in Kluyveromyces. lactis, K. aestuarii, M. fructicola and Zygotorulaspora mraki. We have also identified the four PULgenes in our effective M. pulcherrima strains.

We conducted field studies in three vintages using a specific M. pulcherrima strains. Based on our results, we can state that we have found an effective method to protect grape against B cinerea, which can be applied both in organic cultivation and also before harvest in conventional technology.

Acknowledgements: The authors would like to thank Anita Kovács, Ilona Szilágyi and Szilvia Struba for the profession technical assistance. This research was financed by the grant 2020-1.1.2-PIACI-KFI-2020-00130

References:

1) Krause D.J., Kominek J., Opulente D.A., Shen X.X., Zhou X., Langdon Q.K., DeVirgiliof J., Hulfachora A.B., Kurtzmanf C.P., Hittinger C.T. 2018. Functional and evolutionary characterization of a secondary metabolite gene cluster in budding yeasts. Proc. Natl. Acad. Sci. 115, 11030-11035. DOI: 10.1073/pnas. 1806268115

DOI:

Publication date: October 6, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Zoltán Kállai1*, Kinga Czentye1, Matthias Sipiczki1

1Department of Genetics and Applied Microbiology, Faculty of Science and Technology, University of Debrecen, H-4032 Debrecen, Hungary

Contact the author*

Keywords

crop protection, bioprotection, antagonism, Metschnikowia, Botrytis cinerea 

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Wine without added SO2: Oxygen impact and color evolution during red wine aging

SO2 play a major role in wine stability and evolution during its aging and storage. Winemaking without SO2 is a big challenge for the winemakers since the lack of SO2 affects directly the wine chemical evolution such as the aromas compounds as well as the phenolic compounds. During the red wine aging, phenolic compounds such as anthocyanin, responsible of the red wine colour, and tannins, responsible of the mouthfeel organoleptic properties of wine, evolved quickly from the winemaking process to aging [1]. A lot of new interaction and molecules occurred lead by oxygen[2] thus the lack of SO2 will induce wine properties changes. Nowadays, the phenolic composition of the wine without added SO2 have not been clearly reported.

Development and validation of a free solvent UHPLC/MS-MS method to analyse melatonin and its precursors in Spanish commercial wines  

Melatonin is a bioactive compound present in foods and beverages such as wines. During alcoholic fermentation, yeast transforms tryptophan into certain indole compounds, including melatonin. This paper aims to develop and validate a free solvent analytical method by ultra-high performance liquid chromatography coupled with high resolution mass spectrometry (UHPLC/MS-MS) to determine melatonin and its precursors (L-tryptophan, tryptamine, serotonin, tryptophol, N-acetylserotonin, 5-hydroxytryptophan, and 3- indoleacetic) that appropriately prevent the matrix effect.

Polyphenol content of cork granulates at different steps of the manufacturing process of microagglomerated stoppers treated with supercritical CO2 used for wine bottling

The wine closure industry is mainly divided into three categories: screw caps, synthetic closures, and cork-based closures. Among this latter, microagglomerated cork stoppers treated with supercritical CO2 are now widely used, especially to avoid cork taint contaminations[1]. They are designed with cork granules obtained from cork offcuts of the punching process during the natural cork stoppers production. A previous study[2] showed that these stoppers released fewer polyphenols in 12 % (v/v) hydroalcoholic solution than natural cork stoppers.

Association between dietary pattern and wine consumption and Alzheimer’s disease in a cohort from La Rioja (Spain)

Addressing modifiable risk factors is the most promising strategy to prevent/delay Alzheimer Disease (AD)[1]. The aim of the study was to establish the connections between dietetic habits, wine consumption and AD. Thus, 98 volunteers were recruited: 50 diagnosed as AD and 48 healthy/controls. The Food Frequency Questionnaire (FFQ) was used for dietary patterns assessment and, based on these data, the Mind Diet Score was calculated. (Poly)phenol metabolites (especially derived from wine consumption) were analyzed by UPLC-QqQ-MS/MS in 24-h urine samples to confirm dietary (poly)phenol consumption.

Sensory profile of wines obtained from disease-resistant varieties in La Rioja

The European wine industry is facing multiple challenges derived from climate change and the pressure of different fungal diseases that are compromising the production of traditional varieties. A sustainable alternative maybe the adoption of resistant varieties.
In this study, we have evaluated the enological potential of 9 resistant varieties (5 white and 4 red varieties) in La Rioja. Microvinifications were carried out with three biological replications. Oenological parameters were very diverse with acid content varying from 2.6 g/L to 6.6 g/L.