terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Application of antagonistic Metschnikowia strains against Botrytis cinerea in vineyards 

Application of antagonistic Metschnikowia strains against Botrytis cinerea in vineyards 

Abstract

Less and less chemical plant protection products are approved by the E U. Plant pathogenic fungi become increasingly resistant to the active ingredients that have been around for a long time. Besides, there is a valid demand for effective products that can be applied in organic cultivation.

We examined Metschnikowia strains under laboratory conditions in order to find effective strains against B. cinerea. The antimicrobial mechanism of these yeasts is based on the competition for the ferric ions from the environment. Metschnikowia cells release the pulcherriminic acid which chelates with Fe3+, forming the pigment pulcherrimin. The ability of M. pulcherrima of depleting Fe from the growth media is correlated with it’s ability to inhibit the growth of many microorganism. We compared the strains’ ability to produce pulcherrimin and their ability to inhibit different pathogenic fungi in laboratory conditions. Pulcherrimin production was also reported in the case of other yeast species. Krause et al. (2018) described four genes (PUL1-4) forming a PULcherrimin (PUL) gene cluster present in Kluyveromyces. lactis, K. aestuarii, M. fructicola and Zygotorulaspora mraki. We have also identified the four PULgenes in our effective M. pulcherrima strains.

We conducted field studies in three vintages using a specific M. pulcherrima strains. Based on our results, we can state that we have found an effective method to protect grape against B cinerea, which can be applied both in organic cultivation and also before harvest in conventional technology.

Acknowledgements: The authors would like to thank Anita Kovács, Ilona Szilágyi and Szilvia Struba for the profession technical assistance. This research was financed by the grant 2020-1.1.2-PIACI-KFI-2020-00130

References:

1) Krause D.J., Kominek J., Opulente D.A., Shen X.X., Zhou X., Langdon Q.K., DeVirgiliof J., Hulfachora A.B., Kurtzmanf C.P., Hittinger C.T. 2018. Functional and evolutionary characterization of a secondary metabolite gene cluster in budding yeasts. Proc. Natl. Acad. Sci. 115, 11030-11035. DOI: 10.1073/pnas. 1806268115

DOI:

Publication date: October 6, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Zoltán Kállai1*, Kinga Czentye1, Matthias Sipiczki1

1Department of Genetics and Applied Microbiology, Faculty of Science and Technology, University of Debrecen, H-4032 Debrecen, Hungary

Contact the author*

Keywords

crop protection, bioprotection, antagonism, Metschnikowia, Botrytis cinerea 

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

New varieties descendant from Monastrell with lower sugar and high phenolic content adapted to warm climates

Given that climate change is a continuous process, it is necessary to constantly search for new strategies that help the viticulturist sector to mitigate its consequences. All adaptation strategies will have a greater or lesser effect that in turn will be marked by the times of action. As a long-term action, a genetic breeding program to obtain new varieties descendant from Monastrell has been developed in the Region of Murcia (more specifically, in the IMIDA Research Center) since 1997. In this program, new red varieties have been developed through directed crosses of the Monastrell variety with other varieties such as Cabernet Sauvignon, Tempranillo and Syrah.

Quantifying water use diversity across grapevine rootstock-scion combinations

Vines require proper light levels, temperature, and water availability, and climate change is modifying these factors, hampering yield and quality. Despite the large diversity of rootstocks, varieties, and clones, we still lack knowledge of their combined effects and potential role in a warmer and dryer future. Therefore, we aim to characterize some of the existing diversity of rootstocks and genotypes and their interaction at the eco-physiological level, combining stomatal conductance (gs) and chlorophyll a fluorescence analysis.

Investigating the Ancient Egyptian wines: The wine jars database

In Ancient Egypt, wine was a luxury product consumed mainly by the upper classes and the royal family and offered to gods in daily religious rituals in the temples.
Since the Predynastic (4000-3100 BC) period, wine jars were placed in tombs as funerary offerings. From the Old Kingdom (2680-2160 BC) to the Greco-Roman (332 BC-395 AD) period, viticulture and winemaking scenes were depicted on the private tombs’ walls. During the New Kingdom (1539-1075 BC), wine jars were inscribed to indicate: vintage year, product, quality, provenance, property and winemaker’s name and title.

Genetic prospecting of rainfed viticulture in the region with the largest cultivated area in Chile

The Maule region hosts up to a third of the total area of vineyards in Chile, in an environment where ancient practices inherited from the colonial past coexist with modernity and dynamism that include technified irrigation and fine vines. In the dry land of Maule there is a viticulture that has subsisted with ancient vines and traditions transmitted over generations, and there is little clarity about the origin and classification of the Maule viticulture, giving rise to the use of different concepts as synonyms to describe the ancient, minority, patrimonial or Criollas vines. In order to characterize and protect the ancient material, we studied the genetic diversity of a territorial collection that covers 80% of the communes of the region, prioritizing plants established more than 40-60 years ago.

Characterization of spoilage yeasts from Malbec grapes from San Rafael wine region (Argentina)

The yeast ecosystem in grape musts is quite broad and depends on the region and the health of the grapes. Within this, there are yeasts that can generate fermentative deviations and/or cause defects in the wine. It is very important to address this issue because there are significant economic losses in the wine industry when the fermentation process and/or the organoleptic characteristics of the wine are negatively affected, even more today since climate change has a marked effect on the composition of this ecosystem. The aim of this work is to characterize the behavior regarding detrimental oenological features of potential spoilage yeasts isolated from viticultural environments.