terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Application of antagonistic Metschnikowia strains against Botrytis cinerea in vineyards 

Application of antagonistic Metschnikowia strains against Botrytis cinerea in vineyards 

Abstract

Less and less chemical plant protection products are approved by the E U. Plant pathogenic fungi become increasingly resistant to the active ingredients that have been around for a long time. Besides, there is a valid demand for effective products that can be applied in organic cultivation.

We examined Metschnikowia strains under laboratory conditions in order to find effective strains against B. cinerea. The antimicrobial mechanism of these yeasts is based on the competition for the ferric ions from the environment. Metschnikowia cells release the pulcherriminic acid which chelates with Fe3+, forming the pigment pulcherrimin. The ability of M. pulcherrima of depleting Fe from the growth media is correlated with it’s ability to inhibit the growth of many microorganism. We compared the strains’ ability to produce pulcherrimin and their ability to inhibit different pathogenic fungi in laboratory conditions. Pulcherrimin production was also reported in the case of other yeast species. Krause et al. (2018) described four genes (PUL1-4) forming a PULcherrimin (PUL) gene cluster present in Kluyveromyces. lactis, K. aestuarii, M. fructicola and Zygotorulaspora mraki. We have also identified the four PULgenes in our effective M. pulcherrima strains.

We conducted field studies in three vintages using a specific M. pulcherrima strains. Based on our results, we can state that we have found an effective method to protect grape against B cinerea, which can be applied both in organic cultivation and also before harvest in conventional technology.

Acknowledgements: The authors would like to thank Anita Kovács, Ilona Szilágyi and Szilvia Struba for the profession technical assistance. This research was financed by the grant 2020-1.1.2-PIACI-KFI-2020-00130

References:

1) Krause D.J., Kominek J., Opulente D.A., Shen X.X., Zhou X., Langdon Q.K., DeVirgiliof J., Hulfachora A.B., Kurtzmanf C.P., Hittinger C.T. 2018. Functional and evolutionary characterization of a secondary metabolite gene cluster in budding yeasts. Proc. Natl. Acad. Sci. 115, 11030-11035. DOI: 10.1073/pnas. 1806268115

DOI:

Publication date: October 6, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Zoltán Kállai1*, Kinga Czentye1, Matthias Sipiczki1

1Department of Genetics and Applied Microbiology, Faculty of Science and Technology, University of Debrecen, H-4032 Debrecen, Hungary

Contact the author*

Keywords

crop protection, bioprotection, antagonism, Metschnikowia, Botrytis cinerea 

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Viticultural heritage in mountain territories of Catalonia: prospecting in the region of Osona, northern Spain

The recovery of ancestral or minority vine varieties has been gaining great interest in recent years, among other reasons because it is likely that some of these varieties, due to the fact that they are found in relict areas, have a greater potential for adaptation to external factors (biotic or abiotic) and can minimize the effects that climate change is causing in viticulture. Varieties that can be grown at altitude are currently being sought to combat rising temperatures and prolonged extreme drought conditions. In Catalonia, the Pyrenean expansion of vineyard cultivation is documented from the 10th century and has been related to the “small climatic optimum” (9th-12th centuries) and also to seigniorial power.[1] But different adverse climatic periods and the arrival of Phylloxera by the late 19th century made many of these crops disappear.[2]

Teinturier grapes: Valorization as a source of high-value compounds for the Chilean food industry

The agri-food industry is constantly searching for ingredients of high functional value, healthy and of natural origin. One species of particular interest is Vitis vinifera, due to its recognized antioxidant potential. Among the grape varieties, one group possesses these antioxidant compounds not only in the skin, but also in its pulp: Teinturier. The red grape has traditionally been used for color correction purposes in winemaking, however, its high antioxidant content transforms it into a raw material of high potential for new formulations of ingredients and foods for the health and wellness market.

Typicality of Rioja wines: identification of sensory profiles for the three subregions of DOCa Rioja

Within the DOCa Rioja three main production areas are differentiated: Rioja Alta (RA), Rioja Alavesa (RAv) and Rioja Oriental (RO). They are three diverse territories with particular characteristics that are claimed to give rise to differentiated profiles. The present work aims at evaluating the sensory diversity of young commercial red wines in these three subregions. Therefore 30 young red wines (mainly Tempranillo and vintage 2021), ten from each subregion, were sensory described following a non-verbal free sorting task and a verbal free comment task by 32 well-established Rioja winemakers.

Effect of irrigation in cover cropping vineyards

Cover cropping in vineyard is a sustainable and alternative soil management system to conventional tillage that is gaining more and more importance among winegrowers and is being promoted, among other organizations, by the European Union through the eco-schemes of the Common Agricultural Policy.
However, the use of cover crops in Mediterranean viticultural environments is conditioned, to a large extent, by the availability of irrigation water which, in a context of global warming like the one we are experiencing, must be adjusted to savings strategies, supplying to the vine only what it needs in each moment.

Impact of climate on berry weight dynamics of a wide range of Vitis vinifera cultivars 

In order to study the impact of climate change on Bordeaux grape varieties and to assess the behavior of candidate grape varieties potentially better adapted to the new climatic conditions, an experimental vineyard composed of 52 grape varieties was planted in 2009 at the INRAE Bordeaux Aquitaine center[1]. Among the many parameters studied since 2012, berry weight for each variety was measured weekly from mid-veraison to maturity, with four independent replicates. The kinetics obtained allowed to study berry growth, a key parameter in grape composition and yield.