terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Lipids at the crossroads of protection: lipid signalling in grapevine defence mechanisms

Lipids at the crossroads of protection: lipid signalling in grapevine defence mechanisms

Abstract

Understanding grapevine molecular processes and the underlying defence responses is vital for developing sustainable disease control strategies. Lipid signalling pathways, involving the synthesis and degradation of lipid molecules, have emerged as a key regulator in plant defence against pathogens. This study aims to elucidate the role of fatty acids and lipid signalling in grapevine’s defence response to P. viticola infection. The expression of lipid metabolism-related as well as lipid signalling genes was analysed, by qPCR, in three grapevine genotypes: Chardonnay (susceptible), Regent (tolerant) with Rpv3-1 resistance loci, and Sauvignac (resistant) harbouring a pyramid of Rpv12 and Rpv3-1 resistance loci. A highly aggressive P. viticola isolate (NW-10/16) was employed for the infection studies. Additionally, fatty acid modulation, by gas chromatography, during infection was monitored, by gas chromatography. The work suggests that lipid metabolism and lipid signalling events is genotype-dependent. Notably, Regent displayed specific modulation of genes associated with lipid signalling and fatty acids, possibly linked to the Rpv3 loci. In contrast, Sauvignac, carrying the Rpv12 locus dominantly, may activate alternative defence pathways rather than lipid signalling.

Acknowledgements: The present work was funded by FCT-Portugal: PhD fellowship, (GL: SFRH/BD/145298/2019); Research Units and projects BioISI (UIDB/00006/2020), project (PTDC/BIA-BQM/28539/2017).

1)  Laureano G. et al. (2018) The interplay between membrane lipids and phospholipase A family members in grapevine resistance against Plasmopara viticola. Sci Rep 8, 14538, DOI 10.1038/s41598-018-32559-z

2)  Laureano G. et al. (2023) Grapevine-Associated Lipid Signalling Is Specifically Activated in an Rpv3 Background in Response to an Aggressive P. viticola Pathovar. Cells, 12(3), 394, DOI 10.3390/cells12030394

DOI:

Publication date: October 6, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Gonçalo Laureano1,2*, Ana Rita Matos1, Andreia Figueiredo1,2

1Biosystems & Integrative Sciences Institute (BioISI), Faculdade de Ciências, Universidade de Lisboa, Campo Grande, Lisboa, Portugal
2Grapevine Pathogen Systems Lab, BioISI, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, Lisboa, Portugal

Contact the author*

Keywords

lipid signalling, pathogen interaction, defence, fatty acids

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Analysis of volatile composition of interaction between the pathogen E. necator and two grapevine varieties

Volatile organic compounds (VOCs) are emitted by nearly all plant organs of the plants, including leaves. They play a key role in the communication with other organisms, therefore they are involved in plant defence against phytopathogens. In this study VOCs from grapevine leaves of two varieties of Vitis vinifera infected by Erysiphe necator were analysed. The varieties were selected based on their susceptibility to pathogen, Kishmish Vatkana has the Ren1 resistance gene and Zamarrica showed high susceptibility in previous trials.

Identification of a stable epi-allele associated with flower development and low bunch compactness in a somatic variant of Tempranillo Tinto

Grapevine cultivars are vegetatively propagated to preserve their varietal characteristics. However, spontaneous somatic variations that occur and are maintained during cycles of vegetative growth offer opportunities for the natural improvement of traditional grape cultivars. One advantageous trait for winegrowing is reduced bunch compactness, which decreases the susceptibility to pests and fungal diseases and favor an even berry ripening.

Grapevine cane pruning extract enhances plant physiological capacities and decreases phenolic accumulation in canes and leaves 

Vine cane extracts are a valuable byproduct due to their rich content of polyphenols, vitamins, and other beneficial compounds, which can affect and benefit the vine and the grapes. This study aims to evaluate the response of grapevine plants to irrigation with water supplemented with a vine cane extract, both at physiology response and phenolic composition in different parts of the plant (root, trunk, shoot, leaf, and berry).
Cane extract was obtained by macerating crushed pruning residues with warm water (5:1) and pectolytic enzymes. Two-year-old potted plants were irrigated with water (Control) while others were irrigated with cane extracts, either at 1:4 (w/v, cane extract/water; T 1:4) or at 1:8 (w/v, cane extract/water; T 1:8).

White grape must processed by UHPH as an alternative to SO2 addition: Effect on the phenolic composition in three varieties

The quantity and distribution of polyphenols in musts play a fundamental role in the white winemaking. This is because these substances are exposed to oxidation reactions, which are catalysed by the polyphenol oxidase (PPO), leading to a decrease in the quality of the wines produced. PPO is inactivated by SO2, but currently, due to the restrictions of the legislation, other methodologies are being investigated. Ultra-High Pressure Homogenization (UHPH) is a non-thermal physic technology that exerts an ultrahigh pressure pumping (>200 MPa) of a fluid through a valve in a continuous system.

Acceptability of canned wines: effect of the level of involvement of consumers and type of wine

In recent years there has been a growing demand for alternative packaging designs in the food industry focused on diminishing the carbon footprint. Despite the environmental advantages of cans versus bottles, the traditional environment of wine has hindered the establishment of less contaminant containers. In this context, the objective of this study was to understand and generate knowledge about consumers´ perception of canned wines in comparison to bottled wines.