Lipids at the crossroads of protection: lipid signalling in grapevine defence mechanisms
Understanding grapevine molecular processes and the underlying defence responses is vital for developing sustainable disease control strategies. Lipid signalling pathways, involving the synthesis and degradation of lipid molecules, have emerged as a key regulator in plant defence against pathogens. This study aims to elucidate the role of fatty acids and lipid signalling in grapevine’s defence response to P. viticola infection. The expression of lipid metabolism-related as well as lipid signalling genes was analysed, by qPCR, in three grapevine genotypes: Chardonnay (susceptible), Regent (tolerant) with Rpv3-1 resistance loci, and Sauvignac (resistant) harbouring a pyramid of Rpv12 and Rpv3-1 resistance loci. A highly aggressive P. viticola isolate (NW-10/16) was employed for the infection studies. Additionally, fatty acid modulation, by gas chromatography, during infection was monitored, by gas chromatography. The work suggests that lipid metabolism and lipid signalling events is genotype-dependent. Notably, Regent displayed specific modulation of genes associated with lipid signalling and fatty acids, possibly linked to the Rpv3 loci. In contrast, Sauvignac, carrying the Rpv12 locus dominantly, may activate alternative defence pathways rather than lipid signalling.
Acknowledgements: The present work was funded by FCT-Portugal: PhD fellowship, (GL: SFRH/BD/145298/2019); Research Units and projects BioISI (UIDB/00006/2020), project (PTDC/BIA-BQM/28539/2017).
1) Laureano G. et al. (2018) The interplay between membrane lipids and phospholipase A family members in grapevine resistance against Plasmopara viticola. Sci Rep 8, 14538, DOI 10.1038/s41598-018-32559-z
2) Laureano G. et al. (2023) Grapevine-Associated Lipid Signalling Is Specifically Activated in an Rpv3 Background in Response to an Aggressive P. viticola Pathovar. Cells, 12(3), 394, DOI 10.3390/cells12030394
Issue: ICGWS 2023
1Biosystems & Integrative Sciences Institute (BioISI), Faculdade de Ciências, Universidade de Lisboa, Campo Grande, Lisboa, Portugal
2Grapevine Pathogen Systems Lab, BioISI, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, Lisboa, Portugal