terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Genetic variation among wild grapes native to Japan

Genetic variation among wild grapes native to Japan

Abstract

Domesticated grapes are assumed to have originated in the Middle East. However, a considerable number of species are native in East Asian countries such as China, Korea and Japan as well. Evidence suggests that a total of seven species and eight varieties have been found to be native to Japan.  A wide level variation in morphology, genetic and fruit composition exist in wild grape native to Japan. Hence, the present study aimed to assess the ploidy level and genetic variability among the wild grapes native to Japan. A total of seven wild grape, in addition to two hybrids (Vitis vinifera and Vitis ficifolia (Japanese wild grape)) and one each Vitis vinifera and Vitis labruscana cultivars, were evaluated using 14 random amplified polymorphic DNA (RAPD) markers. The RAPD markers have been reported to be highly variable both within and between species. The RAPD markers were selected simply because of their uniqueness, simplicity and discriminatory capability. Likewise, ploidy level was determined by flow cytometric methods. The flow cytometric analysis showed no variation among wild grapes and their hybrids. All grapes were diploid irrespective of origin and diverse morphological, phenological and berry characteristics. The 14 RAPD primers amplified 120 reproducible bands among 11 grape accessions. Of the 120 total bands, 114 were polymorphic and 6 were monomorphic. The unweighted pair group method of arithmetic averages (UPGMA) using 120 RAPD bands from 14 selected primers clearly separated the wild grapes into distinct groups. The affinity of hybrid grapes with their parents proved true hybridity.

DOI:

Publication date: October 6, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Puspa Raj Poudel1,2*, Ikuo Kataoka3, Kenji Beppu3 and Ryosuke Mochioka2

1Tribhuvan University, Institute of Agriculture and Animal Science, Paklihawa Campus, Siddharthanagar1, Rupandehi, Nepal
2University Farm, Faculty of Agriculture, Kagawa University, Showa, Sanuki, Kagawa 769-2304, Japan
3Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan

Contact the author*

Keywords

wild grapes, RAPD, ploidy level, Vitis ficifolia

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Investigation of cellulose nanofiber-based films used as a protective layer to reduce absorption of smoke phenols into wine grapes

Volatile phenols from wildfire smoke are absorbed by wine grapes, resulting in undesirable smoky and ashy sensory attributes in the affected wine.[1] Unfortunately the severity of wildfires is increasing, particularly when grapes are ripening on the vine. The unwanted flavors of the wine prompted a need for solutions to prevent the uptake of smoke compounds into wine grapes. Films using cellulose nanofibers as the coating forming matrix were developed as an innovative means to prevent smoke phenols from entering Pinot noir grapes. Different film formulations were tested by incorporating low methoxy pectin or chitosan.

Metatranscriptomic analysis of “aszú” berries: the potential role of the most important species of the grape microbiota in the aroma of wines with noble rot

Botrytis cinerea has more than 1200 host plants and is one of the most important plant pathogens in viticulture. Under certain environmental conditions, it can lead to the development of a noble rot, which results in a specific metabolic profile, altering physical texture and chemical composition. The other microbes involved in this process and their functional genes are poorly characterised. We have generated metatranscriptomic [1,2] and DNA metabarcoding data from three months of the Furmint grape variety, representing the four phases of noble rot, from healthy berries to completely dried berries.

Induction of polyphenols in seedlings of Vitis vinifera cv. Monastrell by the application of elicitors

Contamination problems arising from the use of pesticides in viticulture have raised concerns. One of the alternatives to reduce contamination is the use of elicitors, molecules capable of stimulating the natural defences of plants, promoting the production of phenolic compounds (PC) that offer protection against biotic and abiotic stress. Previous studies on Cabernet-Sauvignon seedlings demonstrated that foliar application of elicitors methyl jasmonate (MeJ) and benzothiadiazole (BTH) increased proteins and PC involved in grapevine defence mechanisms. However, no trials had been conducted on Monastrell seedlings, a major winegrape variety in Spain.

Biotic and abiotic factors affecting physiological aspects underlying vegetative vigour in two commercial grapevine varieties

Grapevine vigour, defined as the propensity to assimilate, store and/or use non-structural sugars for allowing fast growth of shoots and producing large canopies[1], is crucial to optimize vineyard management. Recently, a model has been proposed for predicting the vigor of young grapevines through the measurement of the vegetative growth and physiological parameters, such as water status and gas exchange[2]. Our objectives were (1) to explore the influence of the association of two grapevine varieties (Tempranillo and Cabernet Sauvignon, grafted onto R110 rootstocks) with arbuscular mycorrhizal fungi (AMF) on the vegetative vigour of young plants; and (2) to assess the effect of environmental factors linked to climate change on the vegetative vigour of Cabernet Sauvignon.

Development of a new method for detecting acetic acid bacteria in wine

The presence of acetic acid bacteria in wine can lead to the appearance of acetic acid at concentrations above the perception threshold, causing the wine rejection by the consumer. During the winemaking process, avoiding the presence of acetic acid bacteria is very difficult, as there is always a residual population accompanying the wine[1], and the problem arises with the significant development of these microorganisms that metabolizes large amounts of acetic acid.
The concern of wineries to control the presence of acetic acid bacteria in wines during their conservation is due to the absence of simple and effective analyses that allow the detection of these microorganisms in the initial stages.