terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Exploring the genetic diversity of leaf flavonoids content in a set of Iberian grapevine cultivars: preliminary results

Exploring the genetic diversity of leaf flavonoids content in a set of Iberian grapevine cultivars: preliminary results

Abstract

The use of grapevine genetic diversity is a way to mitigate the negative impacts of climate change on viticulture systems. Leaf epidermal flavonoids (including flavonols and anthocyanins) are involved in plant defense mechanisms against environmental stresses, like high temperatures or excessive solar radiation [1,2]. Among other factors, they modulate light absorption, which reduces photoinhibition processes in photosynthetic tissues [1]. Therefore, the identification of grapevine cultivars with an increased content on leaf epidermal flavonoids arises as a potential avenue to improve grapevine tolerance to some detrimental environmental stresses. Here, we analyzed the content in leaf flavonols and leaf anthocyanins at full-veraison in 63 genetically diverse grapevine cultivars from different Iberian regions grown under the same conditions by two alternative optical sensors (Dualex and MPM-100). Both non-invasive systems proved to be able of estimating leaf epidermal flavonoids content in a wide set of cultivars of high genetic diversity, providing highly-correlated results. Whilst we observed a moderate range of variation for leaf flavonols (they were found to vary by a 1.5-fold factor), a higher range of variation was observed for leaf anthocyanins, which varied by a 15.0-fold factor. In addition, this screening allowed us to detect some cultivars with a significant higher content on leaf epidermal flavonols than some widely extended grapevine cultivars, suggested as potential candidates with better adaptation capacity to the expected conditions due to climate change.

Acknowledgements: This work is part of the project “Diversidad genética en la vid y adaptación al cambio climático” (PID2020-120183RB-I00), funded by MCIN/AEI/10.13039/501100011033.

References:

1)  Steyn, W.J. et al. (2002) Anthocyanins in vegetative tissues: a proposed unified function in photoprotection. New Phytol., 155: 349-361, DOI 10.1046/j.1469-8137.2002.00482.x 

2)  Daryanavard, H.et al. (2023) Flavonols modulate plant development, signaling, and stress responses. Curr. Opin. Plant Biol., 72: 102350, DOI: 10.1016/j.pbi.2023.102350

DOI:

Publication date: October 6, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Javier Tello1*, Yolanda Ferradás1,2, Javier Ibáñez1

1 Instituto de Ciencias de la Vid y del Vino, Finca La Grajera, Ctra. de Burgos Km. 6, 26007 Logroño
2 Facultad de Biología, Universidad de Santiago de Compostela, 15872 Santiago de Compostela

Contact the author*

Keywords

anthocyanins, climate change, flavonols, non-invasive phenotyping, Vitis vinifera

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Influence of p-Coumaric Acid and Micronutrients on Growth and 4-Ethylphenol Production by Brettanomyces bruxellensis

The wine spoilage caused by Brettanomyces bruxellensis is one of the global concerns for winemakers. Detecting the presence of B. bruxellensis using routine laboratory culture techniques becomes challenging when cells enter the viable but not culturable (VBNC) state. This study aims to investigate the impact of p-coumaric acid (a volatile phenol precursor) and micronutrients on B. bruxellensis’ culturability, viability, and volatile phenol production under sulfite stress. In red wine, exposure to a high sulfite dose (100.00 mg L-1 potassium metabisulfite) resulted in immediate cell death, followed by a recovery of culturability after two weeks.

Metatranscriptomic analysis of “aszú” berries: the potential role of the most important species of the grape microbiota in the aroma of wines with noble rot

Botrytis cinerea has more than 1200 host plants and is one of the most important plant pathogens in viticulture. Under certain environmental conditions, it can lead to the development of a noble rot, which results in a specific metabolic profile, altering physical texture and chemical composition. The other microbes involved in this process and their functional genes are poorly characterised. We have generated metatranscriptomic [1,2] and DNA metabarcoding data from three months of the Furmint grape variety, representing the four phases of noble rot, from healthy berries to completely dried berries.

Model-assisted analysis of the root traits underlying RSA genotypic diversity in Vitis: a promising approach for rootstock selection?

By dissecting the root system architecture (RSA) into its underpinning components (e.g. root emission, axial growth, radial growth, branching, root direction or tropism) and identifying the relationships between them, functional-structural 3D root models are promising tools for analyzing the diversity and complexity of root system phenotypes with Genotype × Environment interactions. The model parameters are assumed to be synthetic traits, less influenced by the environment, and consequently with less polygenic architectures than the integrative RSA traits they drive. Root models can serve as a basis for in silico development of root system ideotypes by highlighting the developmental processes and parameters that most likely influence RSA fitness.

Applicability of spectrofluorometry and voltammetry in combination with machine learning approaches for authentication of DOCa Rioja Tempranillo wines

The main objective of the work was to develop a simple, robust and selective analytical tool that allows predicting the authenticity of Tempranillo wines from DOCa Rioja. The techniques of voltammetry and absorbance-transmission and fluorescence excitation emission matrix (A-TEEM) spectroscopy have been applied in combination with machine learning (ML) algorithms to classify red wines from DOCa Rioja according to region (Alavesa, Alta or Oriental) and category (young, crianza or reserva).

Nitrogen forms and Iron deficiency: how do Grapevine rootstocks responses change?

Grapevine rootstocks provide protection against environmental biotic and abiotic stresses. Nitrogen (N) and iron (Fe) are growth-limiting factors in many crop plants due to their effects on the chlorophyll and photosynthetic characteristics. Iron nutrition of plants can be significantly affected by different nitrogen forms through altering the uptake ratio of cations and anions, and changing rhizosphere pH. The aim of this study was to investigate the response mechanisms of grapevine rootstocks due to the interaction between different nitrogen forms and iron uptake.