terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Conventional and alternative pest management strategies: a comparative proteomic study on musts

Conventional and alternative pest management strategies: a comparative proteomic study on musts

Abstract

In a context of sustainable agriculture, “agroecological immunity” is an emerging concept to reduce the use of chemical pesticides to protect crops against pathogens. This alternative strategy aims to combine different levers including the use of “bio”solutions. These include biocontrol products, some of which being plant defense elicitors, as well as products authorized in organic farming such as copper or sulfur. In vineyards, depending on climate conditions, powdery and downy mildews can be devastating diseases. So, to guarantee the yield and quality of the harvest, it is usually necessary to treat vines against these diseases from the 5/6-leaf stage to the bunch closure stage. In the present study carried out on a Chardonnay plot located at Lugny (Mâconnais Vineyard, France), we compared, the conventional vineyard protection strategy to the alternative one using “bio” solutions. The latter included the use of copper/sulfur and phosphonate or Bacillus-based products. For the two studied vintages (2020 and 2021), up to 8 treatments were applied whatever the protection strategy used. Besides the evaluation of the protection efficacy against downy and powdery mildews, we performed proteomic analyses (LC-MS/MS) to assess the impact of these two crop protection strategies on must quality. Among the 1041 proteins analyzed, 215 were significantly differentially expressed and the clustering analysis allowed to distinguish the two vintages rather than the protection management strategies. At last, a label-free quantification of proteins using spectral counting was performed from 2021 vintage and finally revealed that less than 2% of proteins were significantly differentially expressed between the two-pest management used.

Acknowledgements: We acknowledge F. Bidaut (Vinipôle Sud Bourgogne, Mâcon, France).

DOI:

Publication date: October 10, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Héloir M-C.1*Ϯ, Lemaitre-Guillier C.1 Ϯ, Schaeffer C.2, Strub J-M.2, Deulvot C.1, Adrian M.1

1Agroécologie, CNRS, INRAE, Institut Agro, Univ. Bourgogne, Univ. Bourgogne Franche-Comté, Dijon, France.
2 Laboratoire de Spectrométrie de Masse Bio-Organique (LSMBO), IPHC, Univ. de Strasbourg, CNRS, Strasbourg, France.

Ϯ: co- first authors

Contact the author*

Keywords

biocontrol, grapevine protection, proteomic analysis, vineyard

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

The tolerance of grapevine rootstocks to water deficit is related to root morphology and xylem anatomy traits 

Climate change is altering water balances, thereby compromising water availability for crops. In grapevine, the strategic selection of genotypes more tolerant to soil water deficit can improve the resilience of the vineyard under this scenario. Previous studies demonstrated that root anatomical and morphological traits determine vine performance under water deficit conditions. Therefore, 13 ungrafted rootstock genotypes, 6 commercial (420 A, 41 B, Evex 13-5, Fercal, 140 Ru y 110 R), and 7 from new breeding programs (RG2, RG3, RG4, RG7, RG8, RG9 and RM2) were evaluated in pots during 2021 and 2022.

Time vs drought: leaf age rather than drought drives osmotic adjustment in V. vinifera cv. Pinot Noir

Global warming and increased frequency and/or severity of drought events are among the most threatening consequences of climate change for agricultural crops. In response to drought, grapevine (as many other plants) exhibits osmotic adjustment through active accumulation of osmolytes which in turn shift the leaf turgor loss point (TLP) to more negative values, allowing to maintain stomata opened at lower water potentials1. We investigated the capacity of Pinot noir leaves to modulate their osmotic potential as a function of: (i) time (seasonal osmoregulation), (ii) growing temperatures, and (iii) drought events, to enhance comprehension of the resilience of grapevines in drought conditions. We performed trails under semi-controlled field conditions, and in two different greenhouse chambers (20/15 °C vs 25/20 °C day/night). For two consecutive vegetative seasons, grafted potted grapevines (Pinot noir/SO4) were subjected to two different water regimes for at least 30 days: well-watered (WW) and water deficit (WD).

Integrative study of Vitis biodiversity for next-generation breeding of grapevine rootstocks 

Drought is one of the main challenges for viticulture in the context of global change. The choice of rootstock could be leveraged for vineyard adaptation to drought as we can improve plant performance without modifying the scion variety. However, most of the existing rootstocks, selected over a century ago, have a narrow genetic background which could compromise their adaptive potential.

Association between dietary pattern and wine consumption and Alzheimer’s disease in a cohort from La Rioja (Spain)

Addressing modifiable risk factors is the most promising strategy to prevent/delay Alzheimer Disease (AD)[1]. The aim of the study was to establish the connections between dietetic habits, wine consumption and AD. Thus, 98 volunteers were recruited: 50 diagnosed as AD and 48 healthy/controls. The Food Frequency Questionnaire (FFQ) was used for dietary patterns assessment and, based on these data, the Mind Diet Score was calculated. (Poly)phenol metabolites (especially derived from wine consumption) were analyzed by UPLC-QqQ-MS/MS in 24-h urine samples to confirm dietary (poly)phenol consumption.

Sparkling wines and atypical aging: investigating the risk of refermentation

Sparkling wine (SW) production entails a two-steps process where grape must undergoes a primary fermentation to produce a base wine (BW) which is then refermented to become a SW. This process allows for the development of a new physicochemical profile characterized by the presence of foam and a different organoleptic profile.