terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Conventional and alternative pest management strategies: a comparative proteomic study on musts

Conventional and alternative pest management strategies: a comparative proteomic study on musts

Abstract

In a context of sustainable agriculture, “agroecological immunity” is an emerging concept to reduce the use of chemical pesticides to protect crops against pathogens. This alternative strategy aims to combine different levers including the use of “bio”solutions. These include biocontrol products, some of which being plant defense elicitors, as well as products authorized in organic farming such as copper or sulfur. In vineyards, depending on climate conditions, powdery and downy mildews can be devastating diseases. So, to guarantee the yield and quality of the harvest, it is usually necessary to treat vines against these diseases from the 5/6-leaf stage to the bunch closure stage. In the present study carried out on a Chardonnay plot located at Lugny (Mâconnais Vineyard, France), we compared, the conventional vineyard protection strategy to the alternative one using “bio” solutions. The latter included the use of copper/sulfur and phosphonate or Bacillus-based products. For the two studied vintages (2020 and 2021), up to 8 treatments were applied whatever the protection strategy used. Besides the evaluation of the protection efficacy against downy and powdery mildews, we performed proteomic analyses (LC-MS/MS) to assess the impact of these two crop protection strategies on must quality. Among the 1041 proteins analyzed, 215 were significantly differentially expressed and the clustering analysis allowed to distinguish the two vintages rather than the protection management strategies. At last, a label-free quantification of proteins using spectral counting was performed from 2021 vintage and finally revealed that less than 2% of proteins were significantly differentially expressed between the two-pest management used.

Acknowledgements: We acknowledge F. Bidaut (Vinipôle Sud Bourgogne, Mâcon, France).

DOI:

Publication date: October 10, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Héloir M-C.1*Ϯ, Lemaitre-Guillier C.1 Ϯ, Schaeffer C.2, Strub J-M.2, Deulvot C.1, Adrian M.1

1Agroécologie, CNRS, INRAE, Institut Agro, Univ. Bourgogne, Univ. Bourgogne Franche-Comté, Dijon, France.
2 Laboratoire de Spectrométrie de Masse Bio-Organique (LSMBO), IPHC, Univ. de Strasbourg, CNRS, Strasbourg, France.

Ϯ: co- first authors

Contact the author*

Keywords

biocontrol, grapevine protection, proteomic analysis, vineyard

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Agronomic behavior of three grape varieties in different planting density and irrigation treatments

In the O Ribeiro Denomination of Origin, there is a winemaking tradition of growing vines under a high-density plantation framework (8,920 vines/ha) and maintaining its vegetative cycle under rainfed conditions.
Currently, viticulture is advancing to plantation frames in which the density is considered medium (5,555 vines/ha), thus allowing mechanized work to be carried out for vineyard management operations. Although, the application of irrigation applied proportionally to the needs of the vegetative cycle of the vine, is a factor that increasingly helps a good development of the vine compared to the summer period, with increasingly uncertain weather forecasts.

Foliar application of urea improved the nitrogen composition of Chenin grapes

The nitrogen composition of the grapes directly affects the developments of alcoholic fermentation and influences the final aromatic composition of the wines. The aim of this study was to determine the effect and efficiency of foliar applications of urea on the nitrogen composition of grapes. This study was carried out during 2023 vintage and in the Chenin vineyard located in Estacion Experimental Mendoza (Argentina). Three urea concentrations 3, 6 and 9 Kg N/ha (C1, C2, and C3, respectively) and control (T) were applied in this vineyard at veraison.

Induction of polyphenols in seedlings of Vitis vinifera cv. Monastrell by the application of elicitors

Contamination problems arising from the use of pesticides in viticulture have raised concerns. One of the alternatives to reduce contamination is the use of elicitors, molecules capable of stimulating the natural defences of plants, promoting the production of phenolic compounds (PC) that offer protection against biotic and abiotic stress. Previous studies on Cabernet-Sauvignon seedlings demonstrated that foliar application of elicitors methyl jasmonate (MeJ) and benzothiadiazole (BTH) increased proteins and PC involved in grapevine defence mechanisms. However, no trials had been conducted on Monastrell seedlings, a major winegrape variety in Spain.

Retrospective analysis of our knowledge regarding the genetics of relevant traits for rootstock breeding 

Rootstocks were the first sustainable and environmentally friendly strategy to cope with a major threat for Vitis vinifera cultivation. In addition to providing Phylloxera resistance, they play an important role in protecting against other soil-borne pests, such as nematodes, and in adapting V. vinifera to limiting abiotic conditions. Today viticulture has to adapt to ongoing climate change whilst simultaneously reducing its environmental impact. In this context, rootstocks are a central element in the development of agro-ecological practices that increase adaptive potential with low external inputs. Despite the apparent diversity of the Vitis genus, only few rootstock varieties are used worldwide and most of them have a very narrow genetic background. This means that there is considerable scope to breed new, improved rootstocks to adapt viticulture for the future.

Acceptability of canned wines: effect of the level of involvement of consumers and type of wine

In recent years there has been a growing demand for alternative packaging designs in the food industry focused on diminishing the carbon footprint. Despite the environmental advantages of cans versus bottles, the traditional environment of wine has hindered the establishment of less contaminant containers. In this context, the objective of this study was to understand and generate knowledge about consumers´ perception of canned wines in comparison to bottled wines.