terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Conventional and alternative pest management strategies: a comparative proteomic study on musts

Conventional and alternative pest management strategies: a comparative proteomic study on musts

Abstract

In a context of sustainable agriculture, “agroecological immunity” is an emerging concept to reduce the use of chemical pesticides to protect crops against pathogens. This alternative strategy aims to combine different levers including the use of “bio”solutions. These include biocontrol products, some of which being plant defense elicitors, as well as products authorized in organic farming such as copper or sulfur. In vineyards, depending on climate conditions, powdery and downy mildews can be devastating diseases. So, to guarantee the yield and quality of the harvest, it is usually necessary to treat vines against these diseases from the 5/6-leaf stage to the bunch closure stage. In the present study carried out on a Chardonnay plot located at Lugny (Mâconnais Vineyard, France), we compared, the conventional vineyard protection strategy to the alternative one using “bio” solutions. The latter included the use of copper/sulfur and phosphonate or Bacillus-based products. For the two studied vintages (2020 and 2021), up to 8 treatments were applied whatever the protection strategy used. Besides the evaluation of the protection efficacy against downy and powdery mildews, we performed proteomic analyses (LC-MS/MS) to assess the impact of these two crop protection strategies on must quality. Among the 1041 proteins analyzed, 215 were significantly differentially expressed and the clustering analysis allowed to distinguish the two vintages rather than the protection management strategies. At last, a label-free quantification of proteins using spectral counting was performed from 2021 vintage and finally revealed that less than 2% of proteins were significantly differentially expressed between the two-pest management used.

Acknowledgements: We acknowledge F. Bidaut (Vinipôle Sud Bourgogne, Mâcon, France).

DOI:

Publication date: October 10, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Héloir M-C.1*Ϯ, Lemaitre-Guillier C.1 Ϯ, Schaeffer C.2, Strub J-M.2, Deulvot C.1, Adrian M.1

1Agroécologie, CNRS, INRAE, Institut Agro, Univ. Bourgogne, Univ. Bourgogne Franche-Comté, Dijon, France.
2 Laboratoire de Spectrométrie de Masse Bio-Organique (LSMBO), IPHC, Univ. de Strasbourg, CNRS, Strasbourg, France.

Ϯ: co- first authors

Contact the author*

Keywords

biocontrol, grapevine protection, proteomic analysis, vineyard

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Characterization of a Sémillon clonal population: exploring genetic diversity, metabolomic profiles, and phenotypic variations

Sémillon is a cultivated grape variety known for contributing to dry and sweet white wine production. However, only seven approved clones have been officially recognized in France[1]. In this study, we aimed to characterize the genetic diversity and metabolomic profiles of a Sémillon clonal population, shedding light on the potential variations within this important grape variety.

Exploring relationships among grapevine chemical and physiological parameters and mycobiome composition under drought stress

Improving our knowledge on biotic and abiotic factors that influence the composition of the grapevine mycobiome is of great agricultural significance, due to potential effects on plant health, productivity, and wine characteristics. Among the various environmental factors affecting the morphological, physiological, biochemical and molecular attributes of grapevine, drought stress is one of the most severe, becoming increasingly an issue worldwide.

Analysis of volatile composition of interaction between the pathogen E. necator and two grapevine varieties

Volatile organic compounds (VOCs) are emitted by nearly all plant organs of the plants, including leaves. They play a key role in the communication with other organisms, therefore they are involved in plant defence against phytopathogens. In this study VOCs from grapevine leaves of two varieties of Vitis vinifera infected by Erysiphe necator were analysed. The varieties were selected based on their susceptibility to pathogen, Kishmish Vatkana has the Ren1 resistance gene and Zamarrica showed high susceptibility in previous trials.

New crossbreed winegrape genotypes cultivated under rainfed conditions in a semi-arid Mediterranean region

Traditional drought tolerant varieties such as Cabernet Sauvignon, Monastrell, and Syrah [1], have been used as parents in the grapevine breeding program initiated by the Instituto Murciano de Investigación y Desarrollo Agrario y Medioambiental (IMIDA) in 1997 [2]. This work presents the results of evaluating three new genotypes obtained from crosses between ‘Monastrell’ and ‘Cabernet Sauvignon’ (MC16 and MC80) and between ‘Monastrell’ and ‘Syrah’ (MS104), comparing their performance under conditions of water scarcity and high temperatures with that of their respective parental varieties. For this purpose, the six genotypes were cultivated under controlled irrigation conditions (60% ETc) and rainfed conditions.

Genetic identification of 200-year-old Serbian grapevine herbarium

Botanist Andreas Raphael Wolny collected a grapevine herbarium from 1812-1824 in Sremski Karlovci (wine region of Vojvodina, Serbia), which represents local cultivated grapevine diversity before the introduction of grape phylloxera in the region. The herbarium comprises over 100 samples organized into two subcollections based on berry colour (red and white varieties), totaling 47 different grape varieties. The objective of this study was to investigate the historical varietal assortment of Balkan and Pannonian winegrowing areas with long viticulture traditions.