terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Conventional and alternative pest management strategies: a comparative proteomic study on musts

Conventional and alternative pest management strategies: a comparative proteomic study on musts

Abstract

In a context of sustainable agriculture, “agroecological immunity” is an emerging concept to reduce the use of chemical pesticides to protect crops against pathogens. This alternative strategy aims to combine different levers including the use of “bio”solutions. These include biocontrol products, some of which being plant defense elicitors, as well as products authorized in organic farming such as copper or sulfur. In vineyards, depending on climate conditions, powdery and downy mildews can be devastating diseases. So, to guarantee the yield and quality of the harvest, it is usually necessary to treat vines against these diseases from the 5/6-leaf stage to the bunch closure stage. In the present study carried out on a Chardonnay plot located at Lugny (Mâconnais Vineyard, France), we compared, the conventional vineyard protection strategy to the alternative one using “bio” solutions. The latter included the use of copper/sulfur and phosphonate or Bacillus-based products. For the two studied vintages (2020 and 2021), up to 8 treatments were applied whatever the protection strategy used. Besides the evaluation of the protection efficacy against downy and powdery mildews, we performed proteomic analyses (LC-MS/MS) to assess the impact of these two crop protection strategies on must quality. Among the 1041 proteins analyzed, 215 were significantly differentially expressed and the clustering analysis allowed to distinguish the two vintages rather than the protection management strategies. At last, a label-free quantification of proteins using spectral counting was performed from 2021 vintage and finally revealed that less than 2% of proteins were significantly differentially expressed between the two-pest management used.

Acknowledgements: We acknowledge F. Bidaut (Vinipôle Sud Bourgogne, Mâcon, France).

DOI:

Publication date: October 10, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Héloir M-C.1*Ϯ, Lemaitre-Guillier C.1 Ϯ, Schaeffer C.2, Strub J-M.2, Deulvot C.1, Adrian M.1

1Agroécologie, CNRS, INRAE, Institut Agro, Univ. Bourgogne, Univ. Bourgogne Franche-Comté, Dijon, France.
2 Laboratoire de Spectrométrie de Masse Bio-Organique (LSMBO), IPHC, Univ. de Strasbourg, CNRS, Strasbourg, France.

Ϯ: co- first authors

Contact the author*

Keywords

biocontrol, grapevine protection, proteomic analysis, vineyard

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

The tolerance of grapevine rootstocks to water deficit is related to root morphology and xylem anatomy traits 

Climate change is altering water balances, thereby compromising water availability for crops. In grapevine, the strategic selection of genotypes more tolerant to soil water deficit can improve the resilience of the vineyard under this scenario. Previous studies demonstrated that root anatomical and morphological traits determine vine performance under water deficit conditions. Therefore, 13 ungrafted rootstock genotypes, 6 commercial (420 A, 41 B, Evex 13-5, Fercal, 140 Ru y 110 R), and 7 from new breeding programs (RG2, RG3, RG4, RG7, RG8, RG9 and RM2) were evaluated in pots during 2021 and 2022.

Distribution and sensory impact of new oak wood-derived compounds in wines

Despite the numerous research studies carried out in recent years, the study of wine aroma remains of great interest due to its complexity. Wine maturation in oak barrels is described as an important step in the production of quality wines. In fact, oak wood develops several aromatic nuances through its toasting which can be released into the wine. A great deal of work has been performed in order to identify the wood-derived volatile compounds that contribute to wine aroma (e.g., whisky-lactone, maltol, eugenol, guaiacol, vanillin).

Advancing grapevine science through genomic research

The seminar will examine the complexities and prospects of genomic research on Vitis species, characterize by exceptionally high heterozygosity and common interspecific gene flow. The seminar will showcase case studies highlighting the critical role of diploid genome references in grape research, specifically in areas such as aroma development, disease resistance, and domestication traits. It will also address the emerging focus on pangenomes within the Vitis genus, particularly in the context of genetic studies on naturally interbreeding populations.

Detoxification capacities of heavy metals and pesticides by yeasts 

Winegrowing is still characterized by the extensive use of chemical fertilizers and plant protection products, despite strong recommendations to limit these practices. A part of these xenobiotics and metals are then found in grape juice and wine, causing a major health concern, as well as negatively affecting the fermentation process. In recent years, there has been renewed interest in non-Saccharomyces yeasts. These species have a wide phenotypic diversity, which would be exploited to broaden the aromatic palette of wines.

Rootstock effect on Cabernet Sauvignon aromatic and chemical composition

Grape quality potential for wine production is strongly influenced by environmental parameters and agronomic factors. Several studies underline the rootstock effect on scions vegetative growth and berry composition [1] with an impact on wine quality. Rootstocks are promising agronomic tools for climate change adaptation and in most grape-growing regions the potential diversity of rootstocks is not fully used and only a few genotypes are planted. Moreover, little is known about the effect of rootstock genetic variability on the aromatic composition in wines.