terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Metabolomic profiling of botrytized grape berries: unravelling the dynamic chemical transformations during noble rot

Metabolomic profiling of botrytized grape berries: unravelling the dynamic chemical transformations during noble rot

Abstract

Botrytis cinerea, a fungal pathogen commonly known as grey mold, which under specific climatic conditions can develop into a desirable form known as noble rot. In this process the fungus penetrates the grape skin, allowing water evaporation and concentration of sugars and flavors, while profoundly affects the metabolite composition of grapes, leading to the production of unique and desirable compounds in the resulting wines. The result is a unique and complex wine with a luscious sweetness, heightened aromatics, and a distinct character. This study aimed to explore the metabolite profiles and chemical transformations associated with noble rot in grape berries from the Betsek area in the Tokaj region. Botrytized grape samples were collected monthly from August to November, covering six phases of botritization. Immediate freezing in liquid nitrogen was performed on-field to preserve sample integrity. Metabolomic analysis was conducted by cryomilling the samples, followed by extraction with methanol and ethyl acetate. The extracts were analyzed using liquid chromatography coupled with quadrupole time-of-flight mass spectrometry, utilizing both positive and negative electrospray ionization. The resulting metabolomic data was processed and statistically analyzed. A principal component analysis (PCA) was performed on the untargeted metabolomic profiles obtained from the botrytized grape samples, which revealed distinct differences between each phase of botritization. The main source of variance observed in the PCA plot was attributed to the botrytization process itself. This finding suggests that the metabolic changes occurring during the different stages of botritization significantly contribute to the overall metabolite composition of the grape berries. Results provided a valuable overview of the dynamic nature of the metabolic transformations associated with noble rot, highlighting the temporal evolution of the metabolite profiles throughout the botrytization process. Further analysis will enable the identification of specific metabolites that contribute to the unique chemical characteristics of noble rot-affected grape berries.

Acknowledgements: This research was funded by the National Research, Development and Innovation Office under the project titled “Research and development to improve sustainability and climate resilience of viticulture and oenology at the Eszterházy Károly Catholic University” with the grant number TKP2021-NKTA-16.

DOI:

Publication date: October 10, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Miklós Lovas1*, Marietta Korózs1, Anna Molnár1, Ádám Hegyi1, Kriszta Szabadi1, Thomas Cels1, Kálmán Zoltán Váczy1

1Research and Development Centre, Eszterházy Károly Catholic University, Eger, Hungary

Contact the author*

Keywords

noble rot, botrytis, metabolomics, grape, LCMS

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Impact of climate on berry weight dynamics of a wide range of Vitis vinifera cultivars 

In order to study the impact of climate change on Bordeaux grape varieties and to assess the behavior of candidate grape varieties potentially better adapted to the new climatic conditions, an experimental vineyard composed of 52 grape varieties was planted in 2009 at the INRAE Bordeaux Aquitaine center[1]. Among the many parameters studied since 2012, berry weight for each variety was measured weekly from mid-veraison to maturity, with four independent replicates. The kinetics obtained allowed to study berry growth, a key parameter in grape composition and yield.

Read More

Uncovering the interplay between Copper and SO2 tolerance in Saccharomyces cerevisiae

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.20.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...
Read More

Grapevine cane pruning extract enhances plant physiological capacities and decreases phenolic accumulation in canes and leaves 

Vine cane extracts are a valuable byproduct due to their rich content of polyphenols, vitamins, and other beneficial compounds, which can affect and benefit the vine and the grapes. This study aims to evaluate the response of grapevine plants to irrigation with water supplemented with a vine cane extract, both at physiology response and phenolic composition in different parts of the plant (root, trunk, shoot, leaf, and berry).
Cane extract was obtained by macerating crushed pruning residues with warm water (5:1) and pectolytic enzymes. Two-year-old potted plants were irrigated with water (Control) while others were irrigated with cane extracts, either at 1:4 (w/v, cane extract/water; T 1:4) or at 1:8 (w/v, cane extract/water; T 1:8).

Read More

Lipids at the crossroads of protection: lipid signalling in grapevine defence mechanisms

Understanding grapevine molecular processes and the underlying defence responses is vital for developing sustainable disease control strategies. Lipid signalling pathways, involving the synthesis and degradation of lipid molecules, have emerged as a key regulator in plant defence against pathogens. This study aims to elucidate the role of fatty acids and lipid signalling in grapevine’s defence response to P. viticola infection. The expression of lipid metabolism-related as well as lipid signalling genes was analysed, by qPCR, in three grapevine genotypes: Chardonnay (susceptible), Regent (tolerant) with Rpv3-1 resistance loci, and Sauvignac (resistant) harbouring a pyramid of Rpv12 and Rpv3-1 resistance loci.

Read More

Biotic and abiotic factors affecting physiological aspects underlying vegetative vigour in two commercial grapevine varieties

Grapevine vigour, defined as the propensity to assimilate, store and/or use non-structural sugars for allowing fast growth of shoots and producing large canopies[1], is crucial to optimize vineyard management. Recently, a model has been proposed for predicting the vigor of young grapevines through the measurement of the vegetative growth and physiological parameters, such as water status and gas exchange[2]. Our objectives were (1) to explore the influence of the association of two grapevine varieties (Tempranillo and Cabernet Sauvignon, grafted onto R110 rootstocks) with arbuscular mycorrhizal fungi (AMF) on the vegetative vigour of young plants; and (2) to assess the effect of environmental factors linked to climate change on the vegetative vigour of Cabernet Sauvignon.

Read More