terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Metabolomic profiling of botrytized grape berries: unravelling the dynamic chemical transformations during noble rot

Metabolomic profiling of botrytized grape berries: unravelling the dynamic chemical transformations during noble rot

Abstract

Botrytis cinerea, a fungal pathogen commonly known as grey mold, which under specific climatic conditions can develop into a desirable form known as noble rot. In this process the fungus penetrates the grape skin, allowing water evaporation and concentration of sugars and flavors, while profoundly affects the metabolite composition of grapes, leading to the production of unique and desirable compounds in the resulting wines. The result is a unique and complex wine with a luscious sweetness, heightened aromatics, and a distinct character. This study aimed to explore the metabolite profiles and chemical transformations associated with noble rot in grape berries from the Betsek area in the Tokaj region. Botrytized grape samples were collected monthly from August to November, covering six phases of botritization. Immediate freezing in liquid nitrogen was performed on-field to preserve sample integrity. Metabolomic analysis was conducted by cryomilling the samples, followed by extraction with methanol and ethyl acetate. The extracts were analyzed using liquid chromatography coupled with quadrupole time-of-flight mass spectrometry, utilizing both positive and negative electrospray ionization. The resulting metabolomic data was processed and statistically analyzed. A principal component analysis (PCA) was performed on the untargeted metabolomic profiles obtained from the botrytized grape samples, which revealed distinct differences between each phase of botritization. The main source of variance observed in the PCA plot was attributed to the botrytization process itself. This finding suggests that the metabolic changes occurring during the different stages of botritization significantly contribute to the overall metabolite composition of the grape berries. Results provided a valuable overview of the dynamic nature of the metabolic transformations associated with noble rot, highlighting the temporal evolution of the metabolite profiles throughout the botrytization process. Further analysis will enable the identification of specific metabolites that contribute to the unique chemical characteristics of noble rot-affected grape berries.

Acknowledgements: This research was funded by the National Research, Development and Innovation Office under the project titled “Research and development to improve sustainability and climate resilience of viticulture and oenology at the Eszterházy Károly Catholic University” with the grant number TKP2021-NKTA-16.

DOI:

Publication date: October 10, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Miklós Lovas1*, Marietta Korózs1, Anna Molnár1, Ádám Hegyi1, Kriszta Szabadi1, Thomas Cels1, Kálmán Zoltán Váczy1

1Research and Development Centre, Eszterházy Károly Catholic University, Eger, Hungary

Contact the author*

Keywords

noble rot, botrytis, metabolomics, grape, LCMS

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Symbiotic microorganisms application in vineyards: impacts on grapevine performance and microbiome

Microorganism-based inoculants have been suggested as a viable solution to mitigate the adverse effects of climate change on viticulture. However, the actual effectiveness of these inoculants when applied under field conditions remains a challenge, and their effects on the existing soil microbiota are still uncertain. This study investigates the impact of arbuscular mycorrhizal fungi inoculation on grapevine performance and microbiome. The study was conducted in a vineyard of Callet cultivar in Binissalem, Mallorca, Spain. Two different treatments were applied: control and inoculation with commercial mycorrhizae complex of Rhizoglomus irregulare applied to plants through irrigation.

Response of red grape varieties irrigated during the summer to water availability at the end of winter in four Spanish wine-growing regions: berry phenolic composition

Water availability is the most limiting factor for vineyard productivity under Mediterranean conditions. Due to the effects caused by the current climate change, wine-growing regions may face serious soil moisture conservation problems, due to the lower water retention capacity of the soil and higher soil irradiation. The aim of this work was to evaluate the effects of soil recharge irrigation in pre-sprouting and summer irrigation every week (30 % ETo) from the pea size state until the end of ripening (RP) compared to exclusively summer irrigation every week (R) in the same way that RP, on berry phenolic composition at harvest.

Defoliation combined with exogenous ABA application results in slower ripening and improved anthocyanin profile

Reducing sugar accumulation in grape (Vitis vinifera L.) berries may be a way to mitigate the effect of climate change. Managing canopy and crop load is an effective way to do so, however, reducing canopy size has been demonstrated to induce undesirable effects on anthocyanins. The aim of this study was to test if an application of exogenous ABA on the grape berries of defoliated vines (⅔ of the leaves removed) can result in slower sugar accumulation while maintaining grape and wine quality. An experiment with defoliation and exogenous ABA application on directly on clusters (factorial design 2×2) was performed with ‘Tempranillo’ fruit-bearing cuttings.

Effects of long-term drought stress on soil microbial communities from a Syrah cultivar vineyard

Changes in the rainfall and temperature patterns affect the increase of drought periods becoming one of the major constraints to assure agricultural and crop resilience in the Mediterranean regions. Beside the adaptation of agricultural practices, also the microbial compartment associated to plants should be considered in the crop management. It is known that the microbial community change according to several factors such as soil composition, agricultural management system, plant variety and rootstock.

Photoprotective extracts from agri-food waste to prevent the effect of light in rosé wines 

Light is responsible for adverse reactions in wine including the formation of unpleasant flavors, loss of vitamins or photodegradation of anthocyanins. Among them, the riboflavin degradation leads to the formation of undesirable volatile compounds, known as light-struck taste. These photo-chemical reactions could be avoided by simply using opaque packaging. However, most rosé wines are kept in transparent bottles due to different commercial reasons. Some agri-food waste extracts have been studied for their photoprotective action which turn to be highly correlated with phenolic content [1].