terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Phenotyping bud break and trafficking of dormant buds from grafted vine

Phenotyping bud break and trafficking of dormant buds from grafted vine

Abstract

In grapevine, phenology from bud break to berry maturation, depends on temperature and water availability. Increases in average temperatures accelerates initiation of bud break, exposing newly formed shoots to detrimental environmental stresses. It is therefore essential to identify genotypes that could delay phenology in order to adapt to the environment. The use of different rootstocks has been applied to change scion’s characteristics, to adapt and resist to abiotic and biotic stresses[1]. It is the main objective of this project to identify rootstock genotypes that could contribute in delaying bud burst in order to adapt to extreme climate events. For this, first we investigated the cold requirements to achieve a homogenous bud break pattern from cuttings of Merlot, Cabernet Sauvignon and Chasselas[2]. Interestingly, Merlot needs longer cold exposure times to achieve 100% bud break. Moreover, bud break of different Vitis species was assessed in the field. Two late and one early Vitis were identified which will be used as rootstock in grafts with Cabernet Sauvignon. Bud break times of these combinations will be assessed to identify changes in bud dormancy in the scion. Furthermore, buds from Cabernet Sauvignon, Merlot and RGM are being sampled for a year-cycle to follow bud development, dormancy and bud break by RNAseq and metabolomics. This, coupled with QTLs identified from bud break of a population of Cabernet Sauvignon x Vitis riparia, will allow the identification of genes involved in dormancy and bud break. Lastly, to understand the rootstock/scion/bud communication, traficking of calceine in cuttings containing dormant and non-dormant buds was studied to indicate the moment in which this molecule is able to enter the bud. Results show that calceine is not able to enter the bud in either condition therefore, 32P is being used to rule out a possible molecular size effect.

Acknowledgements: We thank Lysiane Brocard from the Bordeaux Imaging Center for the advice on microscopy and Bordeaux Plant Sciences from the Université de Bordeaux for funding this research through the Grand Programme de Recherche (GPR).

1)  Miele A. (2019). Rootstock-scion interaction:6. Phenology, chilling and heat requirements of Cabernet Sauvignon grapevine. Revista Brasileira de Fruticultura 41.

2)  Dokoozlian, N.K. 1999. Chilling Temperature and Duration Interact on the Budbreak of ‘Perlette’ Grapevine Cuttings. HORTSCIENCE, VOL. 34(6), OCTOBER 1999.

DOI:

Publication date: October 11, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Anne Marie Labandera Nadeau1*, Elisa Marguerit1, Jean-Pascal Tandonnet1, Coralie Chesseron2, Alain Mollier2, Pierre Gastou1, Marina de Miguel Vega1, Bénédicte Wenden3, Sarah Cookson1

1 Ecophysiology and Functional Genomics of the Grapevine – INRAE Bordeaux Aquitaine
2 Interaction Sol Plante Atmosphére (ISPA) – INRAE Bordeaux Aquitaine

Biologie du Fruit et Pathologie – INRAE Bordeaux Aquitaine

Contact the author*

Keywords

bud break, dormancy, communication, grafting

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Wine odors: chemicals, physicochemical and perceptive processes involved in their perception

The odors of wines are diverse, complex and dynamic and much research has been devoted to the understanding of their chemical bases. However, while the “basic” chemical part of the problem, namely the identity of the chemicals responsible for the different odor nuances, was satisfactorily solved years ago, there are some relevant questions precluding a clear understanding. These questions are related to the physicochemical interactions determining the effective volatilities of the odorants and, particularly, to the perceptual interactions between different odor molecules affecting in different ways to the final sensory outputs.

Preliminary results of water status and metabolite content of three new crossbreed winegrape genotypes

This study presents the preliminary results obtained in 2022, of the evaluation of three new crossbreed winegrape genotypes and their parental varieties, grown under controlled irrigation (60% ETc) and rainfed conditions in a wine-growing area with scarcity of water and high temperatures (Murcia, southeast Spain). The genotypes MC16 and MC80 were obtained from crosses between the varieties ‘Monastrell’ and ‘Cabernet Sauvignon’, and MS104 from crosses between ‘Monastrell’ and ‘Syrah’ [1]. The objective of this study was to analyse the physiological response and vegetative development of the 6 genotypes under the two irrigation conditions, and to study their effect on the content of soluble sugars and chlorophyll in the leaf.

Metabolomic insights into wine’s sensory identity: unveiling climate-driven changes in aroma composition

Wine, a sensitive and intricate agricultural product, is being affected by climate change, which accelerates grapevine phenological stages and alters grape composition and ripening. This influences the synthesis of key aroma compounds, shaping wine’s sensory attributes [1]. The complex aroma profile, resulting from compound interactions, presents a metabolomics challenge to identify these indicators and their environmental change responses, which is being addressed using diverse analytical techniques.

Biotype diversity within the autochthonous ‘Bobal’ grapevine variety

Bobal is the second most widely grown Spanish red grape variety (54,165 has), mainly cultivated in the Valencian Community and especially, in Utiel-Requena region (about 67% of 34,000 has). In this study, agronomic and enological parameters were determined in 98 biotypes selected during 2018 and 2019 in more than 50 vineyards over 50 years-old in the Utiel-Requena region. Moreover, a multi-criteria approach considering temperature and rainfall (Fig. 1A), among other parameters, was made to establish three different zones within the region (Fig. 1B), where in the future the selected biotypes will evaluated. In fact, in 2020, 4 replicates and 12 vines per biotype were planted in an experimental vineyard to preserve this important intra-cultivar diversity.

Development of a new method for detecting acetic acid bacteria in wine

The presence of acetic acid bacteria in wine can lead to the appearance of acetic acid at concentrations above the perception threshold, causing the wine rejection by the consumer. During the winemaking process, avoiding the presence of acetic acid bacteria is very difficult, as there is always a residual population accompanying the wine[1], and the problem arises with the significant development of these microorganisms that metabolizes large amounts of acetic acid.
The concern of wineries to control the presence of acetic acid bacteria in wines during their conservation is due to the absence of simple and effective analyses that allow the detection of these microorganisms in the initial stages.