terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Phenotyping bud break and trafficking of dormant buds from grafted vine

Phenotyping bud break and trafficking of dormant buds from grafted vine

Abstract

In grapevine, phenology from bud break to berry maturation, depends on temperature and water availability. Increases in average temperatures accelerates initiation of bud break, exposing newly formed shoots to detrimental environmental stresses. It is therefore essential to identify genotypes that could delay phenology in order to adapt to the environment. The use of different rootstocks has been applied to change scion’s characteristics, to adapt and resist to abiotic and biotic stresses[1]. It is the main objective of this project to identify rootstock genotypes that could contribute in delaying bud burst in order to adapt to extreme climate events. For this, first we investigated the cold requirements to achieve a homogenous bud break pattern from cuttings of Merlot, Cabernet Sauvignon and Chasselas[2]. Interestingly, Merlot needs longer cold exposure times to achieve 100% bud break. Moreover, bud break of different Vitis species was assessed in the field. Two late and one early Vitis were identified which will be used as rootstock in grafts with Cabernet Sauvignon. Bud break times of these combinations will be assessed to identify changes in bud dormancy in the scion. Furthermore, buds from Cabernet Sauvignon, Merlot and RGM are being sampled for a year-cycle to follow bud development, dormancy and bud break by RNAseq and metabolomics. This, coupled with QTLs identified from bud break of a population of Cabernet Sauvignon x Vitis riparia, will allow the identification of genes involved in dormancy and bud break. Lastly, to understand the rootstock/scion/bud communication, traficking of calceine in cuttings containing dormant and non-dormant buds was studied to indicate the moment in which this molecule is able to enter the bud. Results show that calceine is not able to enter the bud in either condition therefore, 32P is being used to rule out a possible molecular size effect.

Acknowledgements: We thank Lysiane Brocard from the Bordeaux Imaging Center for the advice on microscopy and Bordeaux Plant Sciences from the Université de Bordeaux for funding this research through the Grand Programme de Recherche (GPR).

1)  Miele A. (2019). Rootstock-scion interaction:6. Phenology, chilling and heat requirements of Cabernet Sauvignon grapevine. Revista Brasileira de Fruticultura 41.

2)  Dokoozlian, N.K. 1999. Chilling Temperature and Duration Interact on the Budbreak of ‘Perlette’ Grapevine Cuttings. HORTSCIENCE, VOL. 34(6), OCTOBER 1999.

DOI:

Publication date: October 11, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Anne Marie Labandera Nadeau1*, Elisa Marguerit1, Jean-Pascal Tandonnet1, Coralie Chesseron2, Alain Mollier2, Pierre Gastou1, Marina de Miguel Vega1, Bénédicte Wenden3, Sarah Cookson1

1 Ecophysiology and Functional Genomics of the Grapevine – INRAE Bordeaux Aquitaine
2 Interaction Sol Plante Atmosphére (ISPA) – INRAE Bordeaux Aquitaine

Biologie du Fruit et Pathologie – INRAE Bordeaux Aquitaine

Contact the author*

Keywords

bud break, dormancy, communication, grafting

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Moderate wine consumption – part of a balanced diet or a health risk?

Consumption of wine/alcoholic beverages remains a topic of great uncertainty and controversy worldwide. The term “no safe level” dominates the media communication and policy ever since population studies in 2018 [1,2] were published, which denied the existence of a J-curve and suggested that ANY consumption of an alcoholic beverage is harmful to health. The scientific evidence accumulated during the past decades about the health benefits of moderate wine consumption, were questioned and drinking guidelines considered to be too loose.

Dynamics of Saccharomyces cerevisiae population in spontaneous fermentations from Granxa D’Outeiro terroir (DOP Ribeiro, NW Spain)

Granxa D’Outeiro is a recovered ancient vineyard located in the heart of DOP Ribeiro, where traditional white grapevine varieties are growing under sustainable management. Spontaneous fermentations using grape must from Treixadura, Albariño, Lado, Godello, and Loureira varieties were carried out at experimental winery of Evega. Yeasts were isolated from must and at different stages of fermentation. Those colonies belonging to Saccharomyces cerevisiae were characterized at strain level by mDNA-RFLPs.

Predicting provenance and grapevine cultivar implementing machine learning on vineyard soil microbiome data: implications in grapevine breeding

The plant rhizosphere microbial communities are an essential component of plant microbiota, which is crucial for sustaining the production of healthy crops. The main drivers of the composition of such communities are the growing environment and the planted genotype. Recent viticulture studies focus on understanding the effects of these factors on soil microbial composition since microbial biodiversity is an important determinant of plant phenotype, and of wine’s organoleptic properties. Microbial biodiversity of different wine regions, for instance, is an important determinant of wine terroir.

Sensory profile of wines obtained from disease-resistant varieties in La Rioja

The European wine industry is facing multiple challenges derived from climate change and the pressure of different fungal diseases that are compromising the production of traditional varieties. A sustainable alternative maybe the adoption of resistant varieties.
In this study, we have evaluated the enological potential of 9 resistant varieties (5 white and 4 red varieties) in La Rioja. Microvinifications were carried out with three biological replications. Oenological parameters were very diverse with acid content varying from 2.6 g/L to 6.6 g/L.

Combined use of leaf removal and natural shading to delay grape ripening in Manto negro (Vitis vinifera L.) under deficit irrigation 

The increasingly frequent heat waves during grape ripening pose challenges for premium wine grape production. This makes the development of irrigation and canopy management techniques of great importance to maximize yield and grape quality. A field experiment was carried out during 2021 and 2022 using Manto negro wine grapes to study the effect of two irrigation strategies and different light exposure levels on grape quality.