terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Phenotyping bud break and trafficking of dormant buds from grafted vine

Phenotyping bud break and trafficking of dormant buds from grafted vine

Abstract

In grapevine, phenology from bud break to berry maturation, depends on temperature and water availability. Increases in average temperatures accelerates initiation of bud break, exposing newly formed shoots to detrimental environmental stresses. It is therefore essential to identify genotypes that could delay phenology in order to adapt to the environment. The use of different rootstocks has been applied to change scion’s characteristics, to adapt and resist to abiotic and biotic stresses[1]. It is the main objective of this project to identify rootstock genotypes that could contribute in delaying bud burst in order to adapt to extreme climate events. For this, first we investigated the cold requirements to achieve a homogenous bud break pattern from cuttings of Merlot, Cabernet Sauvignon and Chasselas[2]. Interestingly, Merlot needs longer cold exposure times to achieve 100% bud break. Moreover, bud break of different Vitis species was assessed in the field. Two late and one early Vitis were identified which will be used as rootstock in grafts with Cabernet Sauvignon. Bud break times of these combinations will be assessed to identify changes in bud dormancy in the scion. Furthermore, buds from Cabernet Sauvignon, Merlot and RGM are being sampled for a year-cycle to follow bud development, dormancy and bud break by RNAseq and metabolomics. This, coupled with QTLs identified from bud break of a population of Cabernet Sauvignon x Vitis riparia, will allow the identification of genes involved in dormancy and bud break. Lastly, to understand the rootstock/scion/bud communication, traficking of calceine in cuttings containing dormant and non-dormant buds was studied to indicate the moment in which this molecule is able to enter the bud. Results show that calceine is not able to enter the bud in either condition therefore, 32P is being used to rule out a possible molecular size effect.

Acknowledgements: We thank Lysiane Brocard from the Bordeaux Imaging Center for the advice on microscopy and Bordeaux Plant Sciences from the Université de Bordeaux for funding this research through the Grand Programme de Recherche (GPR).

1)  Miele A. (2019). Rootstock-scion interaction:6. Phenology, chilling and heat requirements of Cabernet Sauvignon grapevine. Revista Brasileira de Fruticultura 41.

2)  Dokoozlian, N.K. 1999. Chilling Temperature and Duration Interact on the Budbreak of ‘Perlette’ Grapevine Cuttings. HORTSCIENCE, VOL. 34(6), OCTOBER 1999.

DOI:

Publication date: October 11, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Anne Marie Labandera Nadeau1*, Elisa Marguerit1, Jean-Pascal Tandonnet1, Coralie Chesseron2, Alain Mollier2, Pierre Gastou1, Marina de Miguel Vega1, Bénédicte Wenden3, Sarah Cookson1

1 Ecophysiology and Functional Genomics of the Grapevine – INRAE Bordeaux Aquitaine
2 Interaction Sol Plante Atmosphére (ISPA) – INRAE Bordeaux Aquitaine

Biologie du Fruit et Pathologie – INRAE Bordeaux Aquitaine

Contact the author*

Keywords

bud break, dormancy, communication, grafting

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Physicochemical behaviour of wine spirit and wine distillate aged in Sherry Casks® and Brandy casks

Brandy is a spirit drink made from “wine spirit” (<86% Alcohol by Volume – ABV; high levels of congeners and they are mainly less volatile than ethanol), it may be blended with a “wine distillate” (<94.8%ABV; low levels of congeners and these are mainly more volatile than ethanol), as long as that distillate does not exceed a maximum of 50% of the alcoholic content of the finished product[1]. Brandy must be aged for at least 6 months in oak casks with <1000L of capacity. During ageing, changes occur in colour, flavour, and aroma that improve the quality of the original distillate.

Tackling the 3D root system architecture of grapevines: a new phenotyping pipeline based on photogrammetry

Plant roots fulfil important functions as they are responsible for the acquisition of water and nutrients, for anchorage and stability, for interaction with symbionts and, in some cases, for the storage of carbohydrates. These functions are associated with the Root System Architecture (RSA, i.e. the form and the spatial arrangement of the roots in the soil). The RSA results from several biological processes (elongation, ramification, mortality…) genetically determined but with high structural plasticity.

Inert gases persistence in wine storage tank blanketing

It is common to find tanks in the winery with wine below their capacity due to wine transfers between tanks of different capacities or the interruption of operations for periods of a few days. This situation implies the existence of an ullage space in the tank with prolonged contact with the wine causing its absorption/oxidation. Oxygen uptake from the air headspace over the wine due to differences in the partial pressure of O2 can be rapid, up to 1.5 mL of O2 per liter of wine in one hour and 100 cm2 of surface area1 and up to saturation after 4 hours.

Vertical cordon training system enhances yield and delays ripening in cv. Maturana Blanca

The growing interest in minority grape varieties is due to their potential for adaptation to global warming and their oenological capabilities. However, the cultivation of these varieties has often been limited due to their low economic efficiency. One such example is Maturana Blanca, a recently recovered and authorized minority grape variety in the DOCa Rioja region, known for its remarkable oenological potential but low productivity. This study aimed to increase the yield of Maturana Blanca by implementing the vertical cordon training system, which allowed for a higher number of buds per plant and an increased cluster count per vine.

Late pruning, an alternative for rainfed vine varieties facing new climatic conditions

In Chile there is a dry farming area known as a traditional wine region, where varieties brought by the Spanish conquerors still persist. These varieties, in general, are cultivated under traditional systems, with low use of technical and economic resources, and low profitability for their grapes and wines. In this region, as in other wine grape growing areas, climatic conditions have changed significantly in recent decades. In particular, the occurrence of spring frosts, when bud break has already begun, have generated significant losses for these growers.