terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Phenotyping bud break and trafficking of dormant buds from grafted vine

Phenotyping bud break and trafficking of dormant buds from grafted vine

Abstract

In grapevine, phenology from bud break to berry maturation, depends on temperature and water availability. Increases in average temperatures accelerates initiation of bud break, exposing newly formed shoots to detrimental environmental stresses. It is therefore essential to identify genotypes that could delay phenology in order to adapt to the environment. The use of different rootstocks has been applied to change scion’s characteristics, to adapt and resist to abiotic and biotic stresses[1]. It is the main objective of this project to identify rootstock genotypes that could contribute in delaying bud burst in order to adapt to extreme climate events. For this, first we investigated the cold requirements to achieve a homogenous bud break pattern from cuttings of Merlot, Cabernet Sauvignon and Chasselas[2]. Interestingly, Merlot needs longer cold exposure times to achieve 100% bud break. Moreover, bud break of different Vitis species was assessed in the field. Two late and one early Vitis were identified which will be used as rootstock in grafts with Cabernet Sauvignon. Bud break times of these combinations will be assessed to identify changes in bud dormancy in the scion. Furthermore, buds from Cabernet Sauvignon, Merlot and RGM are being sampled for a year-cycle to follow bud development, dormancy and bud break by RNAseq and metabolomics. This, coupled with QTLs identified from bud break of a population of Cabernet Sauvignon x Vitis riparia, will allow the identification of genes involved in dormancy and bud break. Lastly, to understand the rootstock/scion/bud communication, traficking of calceine in cuttings containing dormant and non-dormant buds was studied to indicate the moment in which this molecule is able to enter the bud. Results show that calceine is not able to enter the bud in either condition therefore, 32P is being used to rule out a possible molecular size effect.

Acknowledgements: We thank Lysiane Brocard from the Bordeaux Imaging Center for the advice on microscopy and Bordeaux Plant Sciences from the Université de Bordeaux for funding this research through the Grand Programme de Recherche (GPR).

1)  Miele A. (2019). Rootstock-scion interaction:6. Phenology, chilling and heat requirements of Cabernet Sauvignon grapevine. Revista Brasileira de Fruticultura 41.

2)  Dokoozlian, N.K. 1999. Chilling Temperature and Duration Interact on the Budbreak of ‘Perlette’ Grapevine Cuttings. HORTSCIENCE, VOL. 34(6), OCTOBER 1999.

DOI:

Publication date: October 11, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Anne Marie Labandera Nadeau1*, Elisa Marguerit1, Jean-Pascal Tandonnet1, Coralie Chesseron2, Alain Mollier2, Pierre Gastou1, Marina de Miguel Vega1, Bénédicte Wenden3, Sarah Cookson1

1 Ecophysiology and Functional Genomics of the Grapevine – INRAE Bordeaux Aquitaine
2 Interaction Sol Plante Atmosphére (ISPA) – INRAE Bordeaux Aquitaine

Biologie du Fruit et Pathologie – INRAE Bordeaux Aquitaine

Contact the author*

Keywords

bud break, dormancy, communication, grafting

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Influence of p-Coumaric Acid and Micronutrients on Growth and 4-Ethylphenol Production by Brettanomyces bruxellensis

The wine spoilage caused by Brettanomyces bruxellensis is one of the global concerns for winemakers. Detecting the presence of B. bruxellensis using routine laboratory culture techniques becomes challenging when cells enter the viable but not culturable (VBNC) state. This study aims to investigate the impact of p-coumaric acid (a volatile phenol precursor) and micronutrients on B. bruxellensis’ culturability, viability, and volatile phenol production under sulfite stress. In red wine, exposure to a high sulfite dose (100.00 mg L-1 potassium metabisulfite) resulted in immediate cell death, followed by a recovery of culturability after two weeks.

Development of a new method for detecting acetic acid bacteria in wine

The presence of acetic acid bacteria in wine can lead to the appearance of acetic acid at concentrations above the perception threshold, causing the wine rejection by the consumer. During the winemaking process, avoiding the presence of acetic acid bacteria is very difficult, as there is always a residual population accompanying the wine[1], and the problem arises with the significant development of these microorganisms that metabolizes large amounts of acetic acid.
The concern of wineries to control the presence of acetic acid bacteria in wines during their conservation is due to the absence of simple and effective analyses that allow the detection of these microorganisms in the initial stages.

Aroma characterization of mold resistant base wines for sparkling wine produced in a warm-temperate area at two different altitudes

In a recent context where consumers pay an increasing attention to sustainability and eco-friendly aspects in the decision-making process, the use of the resistant varieties in the wine sector have returned to the attention. In this context, the use of mould-resistant grape varieties would be an opportunity for sparkling wine producers as it can reduced the pesticide utilization in grape management and hence production costs.
However, the use of the resistant varieties to produce the base wine may be strongly influenced due to its requirements for a particular balance between sugars and acidity to ensure the quality of the final product. In addition, the aromatic profile of base wine plays a crucial role in the perception of the quality of the sparkling wine.

Characterization of non-cultivated wild grapevines in Extremadura (Spain) 

Several Eurasian wild grapevine populations were found along Extremadura region (southwestern Spain). For conservation and study, one individual from four different populations (named L1, L2, L5 and L6) was vegetatively propagated and planted at Instituto de Investigaciones Agrarias Finca La Orden (CICYTEX), Badajoz. The aim of the present work was to characterize those conserved individuals from four different populations based on both an ampelographic description and a molecular analysis. Three vines per individual were studied.

Assessment of plant water consumption rates under climate change conditions through an automated modular platform

The impact of climate change is noticeable in the present weather, making water scarcity the most immediate mediator reducing the performance and viability of crops, including grapevine (Vitis vinifera L.). The present study developed a system (hardware, firmware, and software) for the determination of plant water use through changes in weight through a period. The aim is to measure the differences in grapevine water consumption in response to climate change (+4oC and 700 ppm) under controlled conditions. The results reveal a correlation between daily plant consumption rates and reference evapotranspiration (ETo).