terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Grapevine adaptation to drought and resistance to Neofusicoccum parvum, causal agent of Botryosphaeria dieback

Grapevine adaptation to drought and resistance to Neofusicoccum parvum, causal agent of Botryosphaeria dieback

Abstract

The sustainability of viticulture in response to climate change has been addressed mainly considering agronomic impacts, such as water management and diseases, either separately or together.

In grapevines, there is strong evidence that different genotypes respond differently to biotic and abiotic stresses. A screening was conducted on various local cultivars in response to drought and Neofusicoum parvum infection aiming to evaluate their susceptibility to abiotic stress and resistance to fungal diseases.

To characterize the varieties’ drought effect, physiological parameters were measured on 12 potted plants of each variety. Relative water content (RWC), leaf water potential (ѰMD) and gas exchange parameters were measured at midday once the plants reached severe water stress levels, i.e. stomatal conductance (gs) between 0,05 and 0,15 mol H2O m-2 s-1.

Moreover, aiming to test the resistance of each variety to the pathogen N. parvum, agar and mycelium disks of 6 mm were placed in a marked wound between the two lower nodes of each plant, using sterile agar disks as controls. Six plants per variety were used as controls and the other 6 were inoculated with N. parvum. Four months after inoculation, the plants were evaluated by measuring the development of internal lesions produced by the fungus.

Under well-watered (WW) conditions, fungal infection provoked a strong reduction in gs and, consequently, an increase in intrinsic water use efficiency (WUEi, AN/gs) in infected plants compared with non-infected plants in all cultivars. However, no other parameters were affected by the fungus. Under water stress (WS) conditions, infection with N. parvum caused similar or even higher gs values in infected than in non-infected plants, thus obtaining similar WUE values for both treatments.

This study may indicate that plants may adjust their physiology to counteract the fungal infection by maintaining a tight stomatal control and by sustaining a balanced carbon change.

DOI:

Publication date: October 11, 2023

Issue: ICGWS 2023

Type: Poster

Authors

David Labarga, Andreu Mairata, Miguel Puelles, David Gramaje, Alicia Pou*

Instituto de Ciencias de la Vid y del Vino (CSIC, Gobierno de la Rioja, Universidad de La Rioja), 26006 Logroño, Spain

Contact the author*

Keywords

grapevine trunk disease, water use efficiency, local cultivars

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Assessing the Effectiveness of Electrodialysis in Controlling Brettanomyces Growth in Wine

Brettanomyces yeast can negatively impact the quality and stability of wines, posing a significant challenge to winemakers. [1] This study aims to develop novel management practices to limit Brettanomyces impact on wines by evaluating the effectiveness of electrodialysis (ED) technology in removing magnesium (Mg2+) from wine to prevent the development of Brettanomyces yeast. The ED technique utilizes charged membranes to extract ions from the wine, and it is considered an alternative to cold stabilization that requires less energy. [2]

Ability of lactic acid bacterial laccases to degrade biogenic amines and OTA in wine

Two of the most harmful microbial metabolites for human health that can be present in wines and either fermented or raw foods are biogenic amines (BA) and ochratoxine A (OTA). Winemakers are aware of the need to avoid their presence in wine by using different strategies, one of them is the use of enzymes. Some recombinant laccases have been characterized and revealed as potential tools to degrade these toxic compounds in wine[1], specifically biogenic amines[2].

Application of DEXI PM Vigne sustainability tool to the assessment of alternative vineyard protection strategies

Implementing alternative grapevine systems that incorporate sustainable strategies and innovative farming practices is essential. However, we lack tools for measuring the impact of these new practices on the overall sustainability of vineyards. DEXi PM Vigne (Gary et al., 2015) is a tool developed for ex ante assessment of the sustainability of grapevine cropping systems, from the plot to the farm scale. In the present study, we focused on implementing new strategies of integrated crop protection management with limited pesticide use in vineyards.

Effect of biological control agents on grapevine rhizosphere microbiome and grapevine defenses

Plant diseases are a major obstacle to crop production. The main approaches to battle plant diseases, consist of synthetic chemicals to attack infecting pathogens. However, concerns are increasing about the effects of chemicals in the environment, leading to an increase in the use of biocontrol agents (BCAs), due to their assets, such as, antagonism, and competition. In this study, we tested the hypothesis that the introduction of Bacillus subtilis PTA-271 (Bs PTA-271) and Trichoderma atroviride SC1 (Ta SC1) produce distinctive modifications in the composition and network structure of the grapevine rhizosphere microbial community, as well as grapevine induced defenses.

Decoupling the effects of water and heat stress on Sauvignon blanc berries

Climate changes have important consequences in viticulture, heat waves accompanied by periods of drought are encountered more and more frequently. This study aims to evaluate the single and combined effect of water deficit and high temperatures on the thiol precursors biosynthesis in Sauvignon blanc grapes. For this purpose, a protocol has been developed for the cultivation of berries on a solid substrate. The berries, collected at three different times starting from veraison and grown in vitro, were subjected to 4 different treatments: control (C), water stress (WS), heat stress (HS), combined water and heat stress (WSHS). Water stress was simulated by adding abscisic acid to the culture medium, while different temperatures, respectively 25°C and 35°C, were managed with two illuminated climatic chambers.