terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Decoupling the effects of water and heat stress on Sauvignon blanc berries

Decoupling the effects of water and heat stress on Sauvignon blanc berries

Abstract

Climate changes have important consequences in viticulture, heat waves accompanied by periods of drought are encountered more and more frequently. This study aims to evaluate the single and combined effect of water deficit and high temperatures on the thiol precursors biosynthesis in Sauvignon blanc grapes. For this purpose, a protocol has been developed for the cultivation of berries on a solid substrate. The berries, collected at three different times starting from veraison and grown in vitro, were subjected to 4 different treatments: control (C), water stress (WS), heat stress (HS), combined water and heat stress (WSHS). Water stress was simulated by adding abscisic acid to the culture medium, while different temperatures, respectively 25°C and 35°C, were managed with two illuminated climatic chambers. The expression of the main genes involved in the biosynthesis of thiol precursors, together with stress marker genes, was evaluated on the berries by qRT-PCR. Moreover, thiol precursors were analysed using an UHPLC/MS method. Results highlighted variable trends in the genes encoding GSTs (glutathione-S-transferase) and GGTs (γ-glutamyl-transferase), responsible for the synthesis of precursors. In detail, the VvGST3 gene was significantly down-regulated in thermal stresses, while HS and WSHS up-regulated the expression of VvGST2, VvGST5, VvGST25 and GGTs. Furthermore, the tested stress marker genes significantly confirm the success of both thermal and water stress conditions. The analysis of thiol precursors, showed that the concentration of Cys-3SH in the two last sampling dates well correlated with the expression of the above-mentioned genes. As opposite, a poor correlation was observed for Glut-3SH. Taken together these results allowed to identify a specific effect of heat and water stress on the regulation of the thiol precursors biosynthetic pathway in Sauvignon blanc berries during maturation.  

DOI:

Publication date: October 11, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Alberto Calderan1,2, Rachele Falchi2, Riccardo Braidotti2, Giorgio Alberti2, Andreja Vanzo3 and Paolo Sivilotti2

1Department of Life Sciences, University of Trieste, via Licio Giorgieri 10, 34127 Trieste, Italy
2Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Via delle     Scienze 206, 33100 Udine, Italy
3Kmetijski Istitut Slovenje (KIS), Hacquetova ulica 17, SI-1000 Ljubljana, Slovenia

Contact the author*

Keywords

water stress, heat stress, climate change, berries culture

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Investigating the Ancient Egyptian wines: The wine jars database

In Ancient Egypt, wine was a luxury product consumed mainly by the upper classes and the royal family and offered to gods in daily religious rituals in the temples.
Since the Predynastic (4000-3100 BC) period, wine jars were placed in tombs as funerary offerings. From the Old Kingdom (2680-2160 BC) to the Greco-Roman (332 BC-395 AD) period, viticulture and winemaking scenes were depicted on the private tombs’ walls. During the New Kingdom (1539-1075 BC), wine jars were inscribed to indicate: vintage year, product, quality, provenance, property and winemaker’s name and title.

Influence of p-Coumaric Acid and Micronutrients on Growth and 4-Ethylphenol Production by Brettanomyces bruxellensis

The wine spoilage caused by Brettanomyces bruxellensis is one of the global concerns for winemakers. Detecting the presence of B. bruxellensis using routine laboratory culture techniques becomes challenging when cells enter the viable but not culturable (VBNC) state. This study aims to investigate the impact of p-coumaric acid (a volatile phenol precursor) and micronutrients on B. bruxellensis’ culturability, viability, and volatile phenol production under sulfite stress. In red wine, exposure to a high sulfite dose (100.00 mg L-1 potassium metabisulfite) resulted in immediate cell death, followed by a recovery of culturability after two weeks.

Inert gases persistence in wine storage tank blanketing

It is common to find tanks in the winery with wine below their capacity due to wine transfers between tanks of different capacities or the interruption of operations for periods of a few days. This situation implies the existence of an ullage space in the tank with prolonged contact with the wine causing its absorption/oxidation. Oxygen uptake from the air headspace over the wine due to differences in the partial pressure of O2 can be rapid, up to 1.5 mL of O2 per liter of wine in one hour and 100 cm2 of surface area1 and up to saturation after 4 hours.

Influence of irrigation frequency on berry phenolic composition of red grape varieties cultivated in four spanish wine-growing regions

The global warming phenomenon involves the frequency of extreme meteorological events accompanied by a change in rainfall distribution. Irrigation frequency (IF) affects the spatial and temporal soil water distribution but its effects on the phenolic composition of the grape have been scarcely studied. The aim of this work was to evaluate the effects of four deficit irrigation frequencies of 30 % ETo: one irrigation per day (T01), two irrigations per week (T03), one irrigation per week (T07) and one irrigation every two weeks (T15) on berry phenolic composition at harvest.

New tool to evaluate color modifications during oxygen consumption in white and red wines

Measuring the effect of oxygen consumption on the color of wines as the level of dissolved oxygen decreases over time is very useful to know how much oxygen a wine can consume without significantly altering its color. The changes produced in wine after being exposed to high oxygen concentrations have been studied by different authors, but in all cases the wine has been analyzed once the oxygen consumption process has been completed. This work presents the results obtained with the use of an equipment designed and made to measure simultaneously the level of dissolved oxygen and the spectrum of the wine, during the oxygen consumption process from saturation levels with air to very low levels, which indicate the total consumption of the dosed oxygen[1,2].