Advancing grapevine science through genomic research

Developments in high-throughput sequencing (HTS) technologies allow us to obtain large amounts of microbial information from wine and must samples. Thus approaches, that are aimed at characterising the microbial diversity during fermentation, can be enhanced, or possibly even replaced, with HTS-based metabarcoding. To reduce experimental biases and increase data reproducibility, we compared 3 DNA extraction methods by evaluating differences in the fungal diversity with Riesling alcoholic fermentation samples at four different vineyards. The fungal diversity profiling was done using the genetic markers ITS2 and D2 using metabarcoding. The extraction methods compared consisted of a commercial kit, a recently published protocol that includes a DNA enhancer, and a protocol based on a buffer containing common inhibitor removal reagents. All methods were able to distinguish vineyard effects on the fungal diversity, but the results differed quantitatively.

Vineyard mechanization will be addressed in this review paper primarily as related to pruning and harvesting since these operations typically require a great deal of the total yearly labour demand (Intrieri and Poni, 1998). However, to be able to define how mechanization interacts with “terroir”, a rigorous definition of the latter term is needed.

to maintain viticulture under global warming conditions, it is important to carefully select the appropriate genotypes for each vine-growing region and develop cultivars that are drought resistant. this ability is highly dependent on hydraulic traits, which are dynamic and vary according to the vine’s developmental stage and climatic conditions. this framework steadily enhances our understanding of the differences in drought resistance among vitis genotypes. however, there is still a need to comprehensively grasp the intra-specific variability, particularly between oenological and table grape cultivars.

Under standard wine tasting conditions, volatile organic compounds (VOCs) responsible for the wine’s bouquet progressively invade the glass headspace above the wine surface. Most of wines being complex water/ethanol mixtures (with typically 10-15 % ethanol by volume), gaseous ethanol is therefore undoubtedly the most abundant VOC in the glass headspace [1]. Yet, gaseous ethanol is known to have a multimodal influence on wine’s perception [2]. Of particular importance to flavor perception is the effect of ethanol on the release of aroma compounds into the headspace of the beverage [1].

The wine-making process generates numerous by-products at each stage (crushing, fermentation, ageing), including wine lees, which account for almost 25% of the total quantity.