terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Differences in metabolism among species and hybrids of the genus Saccharomyces during wine fermentation unveiled by multi-omic analysis 

Differences in metabolism among species and hybrids of the genus Saccharomyces during wine fermentation unveiled by multi-omic analysis 

Abstract

Yeast species S. cerevisiae, S. uvarum, S. kudriavzevii and their hybrids present clear metabolic differences, even when we compared S. cerevisiae wine versus wild strain. These species and hybrids produced significantly higher amounts of glycerol, organic acids, 2,3-butanediol, and 2-phenyl ethanol and a reduction of the ethanol yield, properties very interesting in the sector to deal with climate change effects. To understand the existing differences, we have used several omics techniques to analyze the dynamics of the (intra- and extracellular) metabolomes and/or transcriptomes of representative strains of S. cerevisiae, S. uvarum, S. kudriavzevii, and hybrids. These data indicate that the NADH/NADH+ cofactor regeneration is different among these species. We also observed that these species produce more erythritol, never described before as a by-product in S. cerevisiae. Using phylogenetic and genetic comparative approaches with Y. lipolytica erythrose reductases, we demonstrated that ΔGRE3 was the single mutant that decreased erythritol production.

Related to the ethanol yield, by whole genome comparative analysis, we have detected an ADH2 allele specific to the wine strains, derived from an ADH1ADH2 gene conversion. This allele results in a lower affinity for ethanol and a higher affinity for acetaldehyde and provides an advantage over other strains in wine fermentation.

Finally, we will explain how can we apply this knowledge to optimize the wine processes using digital twins.

Acknowledgements: PID2021-126380OB-C31 and PID2021-126380OB-C33, AGROALNEXT/2022/021, PLEC2021-007827; MCIN/AEI/10.13039/501100011033, as a ‘Severo Ochoa’ Center of Excellence (CEX2021-001189-S).

DOI:

Publication date: October 19, 2023

Issue: ICGWS 2023

Type: Article

Authors

Amparo Querol1*; Sonia Albillos 1; Romain Minebois1; Eva Balsa-Canto 3; Alba Contreras1, Lainy Ramirez-Aroca1; Eladio Barrio 1,2

1 Food Biotechnology Department (IATA-CSIC), Paterna, Spain
2 Genetics Department (University of Valencia), Valencia, Spain
3 Biosistemas e Ingeniería de Bioprocesos, IIM-CSIC, Vigo, Spain

Contact the author*

Keywords

Saccharomyces, wine fermentation, kinetic and genome-scale metabolic model; digital twin

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

New varieties descendant from Monastrell with lower sugar and high phenolic content adapted to warm climates

Given that climate change is a continuous process, it is necessary to constantly search for new strategies that help the viticulturist sector to mitigate its consequences. All adaptation strategies will have a greater or lesser effect that in turn will be marked by the times of action. As a long-term action, a genetic breeding program to obtain new varieties descendant from Monastrell has been developed in the Region of Murcia (more specifically, in the IMIDA Research Center) since 1997. In this program, new red varieties have been developed through directed crosses of the Monastrell variety with other varieties such as Cabernet Sauvignon, Tempranillo and Syrah.

Identification of important genomic regions controlling resistance to biotic and abiotic stresses in Vitis sp. through QTL meta-analysis

In the context of global change, the environmental conditions are expected to be more stressful for viticulture. The choice of the rootstock may play a crucial role to improve the adaptation of viticulture to new biotic and abiotic threats (Ollat et al., 2016). However, the selection of interesting traits in rootstock breeding programs is complex because of the combination of multiple targets in a same ideotype. In this sense, the integration of studies about the genetic architecture for desired biotic and abiotic response traits allow us to identify genomic regions to combine and those with interesting pleiotropic effects.

The colour pattern of flower arrangements influence wine tasters’ sensory description

The arrangements of flowers and wine counterparts are inextricably linked. Whether a fundamental aspect of tablescaping or acolytes to broader entertainment rituals, they have an entangled history since ancient times. The aim of this contribution is to verify the influence of visually delicate and robust flower arrangements on individual description of wines. Changes in the sensory description of wines were investigated during subjects’ (thirty-two participants) exposure to three different conditions: the presence of delicate, robust, or totally absent flower arrangements. In each condition, the same two wines were blind tasted: a wine previously defined as delicate – a Pinot Noir from Australia, and a wine known for its robust character – a Tannat from Uruguay.

The interplay between water deficit and nitrogen and potassium nutrition in Vitis vinifera L.

Climate change is expected to provoke an increase in the frequency and intensity of drought events and water scarcity that will have detrimental effects on photosynthesis and plant yield. To sustain an appropriate plant yield under sub-optimal conditions, a common practice is the application of high amounts of fertilizers with negative environmental consequences. The present study aims at evaluating the interplay between water and nutrient availability, namely nitrogen (N) and potassium (K), in two grapevine cultivars with a different sensitivity to water shortage stress. Two-year-old Vitis Vinifera cv. Cabernet Sauvignon and Grenache grapevine plants grafted on SO4 rootstock have been transferred in pots under semi-environmental conditions.

Potential of new genetic resources to improve drought adaptation of grapevine rootstocks

Grapevines are grown mainly as grafts worldwide, but the rootstocks most commonly used were selected between the late 19th and early 20th centuries and are based on reduced genetic diversity[1]. In the context of climate change, it is indeed urgent to diversify the range of rootstocks with genotypes much more adapted to drier environments, than the existing ones[2]. The aim of this study was to evaluate the potential of new genetic resources for grapevine rootstock breeding programs. For this purpose, 12 American and Asian wild Vitis species (3 to 5 accessions per species = 50 accessions) were evaluated for their rooting ability and drought response.