terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Differences in metabolism among species and hybrids of the genus Saccharomyces during wine fermentation unveiled by multi-omic analysis 

Differences in metabolism among species and hybrids of the genus Saccharomyces during wine fermentation unveiled by multi-omic analysis 

Abstract

Yeast species S. cerevisiae, S. uvarum, S. kudriavzevii and their hybrids present clear metabolic differences, even when we compared S. cerevisiae wine versus wild strain. These species and hybrids produced significantly higher amounts of glycerol, organic acids, 2,3-butanediol, and 2-phenyl ethanol and a reduction of the ethanol yield, properties very interesting in the sector to deal with climate change effects. To understand the existing differences, we have used several omics techniques to analyze the dynamics of the (intra- and extracellular) metabolomes and/or transcriptomes of representative strains of S. cerevisiae, S. uvarum, S. kudriavzevii, and hybrids. These data indicate that the NADH/NADH+ cofactor regeneration is different among these species. We also observed that these species produce more erythritol, never described before as a by-product in S. cerevisiae. Using phylogenetic and genetic comparative approaches with Y. lipolytica erythrose reductases, we demonstrated that ΔGRE3 was the single mutant that decreased erythritol production.

Related to the ethanol yield, by whole genome comparative analysis, we have detected an ADH2 allele specific to the wine strains, derived from an ADH1ADH2 gene conversion. This allele results in a lower affinity for ethanol and a higher affinity for acetaldehyde and provides an advantage over other strains in wine fermentation.

Finally, we will explain how can we apply this knowledge to optimize the wine processes using digital twins.

Acknowledgements: PID2021-126380OB-C31 and PID2021-126380OB-C33, AGROALNEXT/2022/021, PLEC2021-007827; MCIN/AEI/10.13039/501100011033, as a ‘Severo Ochoa’ Center of Excellence (CEX2021-001189-S).

DOI:

Publication date: October 19, 2023

Issue: ICGWS 2023

Type: Article

Authors

Amparo Querol1*; Sonia Albillos 1; Romain Minebois1; Eva Balsa-Canto 3; Alba Contreras1, Lainy Ramirez-Aroca1; Eladio Barrio 1,2

1 Food Biotechnology Department (IATA-CSIC), Paterna, Spain
2 Genetics Department (University of Valencia), Valencia, Spain
3 Biosistemas e Ingeniería de Bioprocesos, IIM-CSIC, Vigo, Spain

Contact the author*

Keywords

Saccharomyces, wine fermentation, kinetic and genome-scale metabolic model; digital twin

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

The colour pattern of flower arrangements influence wine tasters’ sensory description

The arrangements of flowers and wine counterparts are inextricably linked. Whether a fundamental aspect of tablescaping or acolytes to broader entertainment rituals, they have an entangled history since ancient times. The aim of this contribution is to verify the influence of visually delicate and robust flower arrangements on individual description of wines. Changes in the sensory description of wines were investigated during subjects’ (thirty-two participants) exposure to three different conditions: the presence of delicate, robust, or totally absent flower arrangements.

Photoprotective extracts from agri-food waste to prevent the effect of light in rosé wines 

Light is responsible for adverse reactions in wine including the formation of unpleasant flavors, loss of vitamins or photodegradation of anthocyanins. Among them, the riboflavin degradation leads to the formation of undesirable volatile compounds, known as light-struck taste. These photo-chemical reactions could be avoided by simply using opaque packaging. However, most rosé wines are kept in transparent bottles due to different commercial reasons. Some agri-food waste extracts have been studied for their photoprotective action which turn to be highly correlated with phenolic content [1].

Toasting and grain effect on Tempranillo red wine aged in Quercus petraea barrels

The barrel-making process is widely recognized as a crucial practice that affects the composition of barrel-aged wine. After the drying process, the staves are considered ready for barrel assembly, which includes the processes of bending and toasting the barrel structure. Toasting is considered one of the most critical stages in determining the physical and chemical composition of the staves, which can influence the chemical and sensory composition of the wine aged in barrels made from them [1].

Biotic and abiotic factors affecting physiological aspects underlying vegetative vigour in two commercial grapevine varieties

Grapevine vigour, defined as the propensity to assimilate, store and/or use non-structural sugars for allowing fast growth of shoots and producing large canopies[1], is crucial to optimize vineyard management. Recently, a model has been proposed for predicting the vigor of young grapevines through the measurement of the vegetative growth and physiological parameters, such as water status and gas exchange[2]. Our objectives were (1) to explore the influence of the association of two grapevine varieties (Tempranillo and Cabernet Sauvignon, grafted onto R110 rootstocks) with arbuscular mycorrhizal fungi (AMF) on the vegetative vigour of young plants; and (2) to assess the effect of environmental factors linked to climate change on the vegetative vigour of Cabernet Sauvignon.

Lipids at the crossroads of protection: lipid signalling in grapevine defence mechanisms

Understanding grapevine molecular processes and the underlying defence responses is vital for developing sustainable disease control strategies. Lipid signalling pathways, involving the synthesis and degradation of lipid molecules, have emerged as a key regulator in plant defence against pathogens. This study aims to elucidate the role of fatty acids and lipid signalling in grapevine’s defence response to P. viticola infection. The expression of lipid metabolism-related as well as lipid signalling genes was analysed, by qPCR, in three grapevine genotypes: Chardonnay (susceptible), Regent (tolerant) with Rpv3-1 resistance loci, and Sauvignac (resistant) harbouring a pyramid of Rpv12 and Rpv3-1 resistance loci.