terclim by ICS banner
IVES 9 IVES Conference Series 9 YEAST-PRODUCED VOLATILES IN GRAPE BASED SYSTEM MODEL ACTING AS ANTIFUNGAL BIOAGENTS AGAINST PHYTOPATHOGEN BOTRYTIS CINEREA

YEAST-PRODUCED VOLATILES IN GRAPE BASED SYSTEM MODEL ACTING AS ANTIFUNGAL BIOAGENTS AGAINST PHYTOPATHOGEN BOTRYTIS CINEREA

Abstract

Botrytis cinerea Pers., the causal agent of grey mould disease, is responsible for substantial economic losses, as it causes reduction of grape and wine quality and quantity. Exploitation of antagonistic yeasts is a promising strategy for controlling grey mould incidence and limiting the usage of synthetic fun- gicides. In our previous studies, 119 different indigenous yeasts were screened for putative multidi- mensional modes of action against filamentous fungus B. cinerea [1]. The most promissing biocontrol yeast was Pichia guilliermondii ZIM624, which exhibited several anatagonistic traits (production of cell wall degrading enzymes, chitinase and β-1,3-glucanase; demonstration of in vitro inhibitory effect on B. cinerea mycelia radial growth; production of antifungal volatiles, assimilation of a broad diversity of carbon sources, contributing to its competitivnes in inhabiting grapes in nature). In addition, P. guilliermondii ZIM 624 possessed interesting enological traits, did not produce off-flavor related H2S and appeared as β-lyase and β-glucosidase producer.

Accordingly, the aim of this researsch was to study the antifungal mechanisms by assessing the volatiles produced by P. guilliermondii ZIM624. Namely, a study was conducted to identify volatile organic com- pounds (4 higher alcohols, 6 volatile phenols, 23 esters and 27 terpenes) produced by antagonistic Pichia guilliermondii strain ZIM624 and to determine the efficacy of the chosen volatiles of P. guilliermondii in suppression of B. cinerea growth and control of Botrytis fruit rot of grape berries. Thereby, a comprehensive assessment of produced volatiles in the process of wine production was achieved using two validated analytical methods (one for terpenes and one for the rest of mentioned volatiles) comprised of automated headspace (HS) solid-phase microextraction (SPME) and gas chromatography coupled with mass spectrometric detection (GC-MS). Both methods were developed based on already published me- thod for determionation of volatiles in wine samples [2]. Among identified volatiles, 13 yeast-produced volatiles were selected and their antifungal activity was tested against B. cinerea in the fumigation bioassay. Terpenes citronellol, geraniol, nerol, α-terpineol and linalool were the most effective against B. cinerea mycelium growth with the EC50 beetwen 6,6 to 32,8 μL/L. 4-Vinyl phenol and isoamyl ace- tate also effectively inhibited mycelial growth of B. cinerea, EC50 being 48,6 and 63,3 μL/L, respectively, followed by eucalyptol (EC50 201,6 μL/L) and ethyl butyrate (EC50 238,4 μL/L). 4-Vinyl guaiacol did not show any inhibitory effect, while the remaining tested compounds showed inhibition against B. cinerea growth, however we were not able to determine EC50 with the selected concentration ranges. Additio- naly, exposure of B. cinerea-infected grape berries to the volatiles from P. guilliermondii cultures also lowered the number of infected grape berries, when applied to in vivo assay.

Herein presented novel research approach strongly suggests that yeast produced volatiles such as ter- penes, volatile phenols and esters are one of the possible mechanisms for controlling Botrytis rot of fruit and promising biofumigants.

1. Adesida R. 2022. Exploration of yeast biodiversity potential for development of alternative biofungicides in viticulture : dissertation. University of Nova Gorica.
2. Antalick, G.; Tempère, S.; Šuklje, K.; Blackman, J.W.; Deloire, A.; Revel,, G.; Schmidtke, L. M. Investigation and Sensory Characterization of 1,4-Cineole: A Potential Aromatic Marker of Australian Cabernet Sauvignon Wine. (2015), J Agric Food Chem 63(41): 9103-11

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Article

Authors

Mitja Martelanc1, Lenart Žežlina2, Tatjana Radovanović Vukajlović1, Melita Sternad Lemut1, Lorena Butinar1

1. University of Nova Gorica, Wine Research Centre, Glavni trg 8, 5271 Vipava, Slovenia
2. University of Ljubljana, Biotechnical Faculty, Jamnikarjeva ulica 101, 1000 Ljubljana, Slovenia

Contact the author*

Keywords

volatile organic compounds, HS-SPME-GC-MS, biocontrol, Botrytis cinerea

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

EFFECTS OF INDUCED SUNBURN DAMAGES ON WHITE WINE PROPERTIES

Climate change is a great challenge for the environment and affects the wine industry as well. Sunburn damage of sensitive grapes increase with severe heat periods. Besides significant loss of yield sunburn, modifies sensory properties of the wines and may cause climate-related off-flavours. To initiate sunburn in a controlled way, in 2021 sunburn was directly induced in the vineyard with the GrapeBurner device, exposing grapes of the varieties Riesling and Pinot Blanc with UV and IR radiation. This device was first assembled by Kai Müller of the university in Geisenheim and consists of a carriage with 6 UV/IR lamps. A 15 min irradiation was applied in early September at 60°Oe. Due to the colder season in 2021 the grapes were not harmed by previous sunburn damage.

UNRAVELING THE CHEMICAL MECHANISM OF MND FORMATION IN RED WINE DURING BOTTLE AGING : IDENTIFICATION OF A NEW GLUCOSYLATED HYDROXYKETONE PRO-PRECURSOR

During bottle aging, the development of wine aroma through low and gradual oxygen exposure is often positive in red wines, but can be unfavorable in many cases, resulting in a rapid loss of fresh, fruity flavors. Prematurely aged wines are marked by intense prune and fig aromatic nuances that dominate the desirable bouquet achieved through aging (Pons et al., 2013). This aromatic defect, in part, is caused by the presence of 3-methyl-2,4-nonanedione (MND). MND content was shown to be lower in nonoxidized red wines and higher in oxidized red wines, which systematically exceeds the odor detection threshold (62 ng/L).

DETERMINATION OF MINERAL COMPOSITION IN CV. TERAN (VITIS VINIFERA L.) RED WINE AFFECTED BY PRE-FERMENTATIVE MASH COOLING, HEATING, SAIGNÉE TECHNIQUE AND PROLONGED POST-FERMENTATIVE MACERATIONS

This study aimed to determine mineral composition in red wine obtained from cv. Teran (Vitis vinifera L.), autochtonous Croatian grape variety. Six different vinification treatments, including the control treatment (7-day standard maceration), were performed to study the effects of: 48-hour pre-fermentative mash cooling (8 °C) followed by prolonged post-fermentative maceration of 13 days (C15), 28 days (C30), and saignée technique (juice runoff) proceeded with prolonged post-fermentative maceration of 13 days (CS15); and effect of 48-hour heating (50 °C) followed by prolonged post-fermentative maceration of 13 days (H15) and 28 days (H30) on macro- and microelements in wine.

DISCRIMINATION OF BOTRYTIS CINEREA INFECTED GRAPES USING UNTARGE-TED METABOLOMIC ANALYSIS WITH DIRECT ELECTROSPRAY IONISATION MASS SPECTROMETRY

Infection of grapes (Vitis vinifera) by Botrytis cinerea (grey mould) is a frequent occurrence in vineyards and during prolonged wet and humid conditions can lead to significant detrimental impact on yield and overall quality. Growth of B. cinerea causes oxidisation of phenolic compounds resulting in a loss of colour and formation of a suite of off-flavours and odours in wine made from excessively infected fruit. Apart from wine grapes, developing post-harvest B. cinerea infection in high-value horticultural products during storage, shipment and marketing may cause significant loss in fresh fruits, vegetables and other crops. A rapid and sensitive assessment method to detect, screen and quantify fungal infection would greatly assist viticultural growers and winemakers in determining fruit quality.

INFLUENCES OF SO2 ADDITION AND STORAGE CONDITIONS IN THE DETERMINATION OF MEAN DEGREE OF POLYMERIZATION OF PROANTHOCYANIDINS IN AGED RED WINES

The structural diversity is one of the most remarkable characteristics of proanthocyanidins (PA). Indeed, PA in wines may vary in the B-ring and C-ring substitutes, the C-ring stereochemistry, the degree of polymerization (DP) and the linkage between the monomers. Knowing in detail the structural characteristics of the PA of a wine can help us to understand and modulate several sensorial characteristics of the wine, such as color, antioxidant properties, flavor, and mouthfeel properties. In the last years was discovered and confirmed the presence of sulfonated monomeric and oligomeric flavan-3-ols in wine [1], as well as was pointed out their importance in wine quality [1,2].