terclim by ICS banner
IVES 9 IVES Conference Series 9 YEAST-PRODUCED VOLATILES IN GRAPE BASED SYSTEM MODEL ACTING AS ANTIFUNGAL BIOAGENTS AGAINST PHYTOPATHOGEN BOTRYTIS CINEREA

YEAST-PRODUCED VOLATILES IN GRAPE BASED SYSTEM MODEL ACTING AS ANTIFUNGAL BIOAGENTS AGAINST PHYTOPATHOGEN BOTRYTIS CINEREA

Abstract

Botrytis cinerea Pers., the causal agent of grey mould disease, is responsible for substantial economic losses, as it causes reduction of grape and wine quality and quantity. Exploitation of antagonistic yeasts is a promising strategy for controlling grey mould incidence and limiting the usage of synthetic fun- gicides. In our previous studies, 119 different indigenous yeasts were screened for putative multidi- mensional modes of action against filamentous fungus B. cinerea [1]. The most promissing biocontrol yeast was Pichia guilliermondii ZIM624, which exhibited several anatagonistic traits (production of cell wall degrading enzymes, chitinase and β-1,3-glucanase; demonstration of in vitro inhibitory effect on B. cinerea mycelia radial growth; production of antifungal volatiles, assimilation of a broad diversity of carbon sources, contributing to its competitivnes in inhabiting grapes in nature). In addition, P. guilliermondii ZIM 624 possessed interesting enological traits, did not produce off-flavor related H2S and appeared as β-lyase and β-glucosidase producer.

Accordingly, the aim of this researsch was to study the antifungal mechanisms by assessing the volatiles produced by P. guilliermondii ZIM624. Namely, a study was conducted to identify volatile organic com- pounds (4 higher alcohols, 6 volatile phenols, 23 esters and 27 terpenes) produced by antagonistic Pichia guilliermondii strain ZIM624 and to determine the efficacy of the chosen volatiles of P. guilliermondii in suppression of B. cinerea growth and control of Botrytis fruit rot of grape berries. Thereby, a comprehensive assessment of produced volatiles in the process of wine production was achieved using two validated analytical methods (one for terpenes and one for the rest of mentioned volatiles) comprised of automated headspace (HS) solid-phase microextraction (SPME) and gas chromatography coupled with mass spectrometric detection (GC-MS). Both methods were developed based on already published me- thod for determionation of volatiles in wine samples [2]. Among identified volatiles, 13 yeast-produced volatiles were selected and their antifungal activity was tested against B. cinerea in the fumigation bioassay. Terpenes citronellol, geraniol, nerol, α-terpineol and linalool were the most effective against B. cinerea mycelium growth with the EC50 beetwen 6,6 to 32,8 μL/L. 4-Vinyl phenol and isoamyl ace- tate also effectively inhibited mycelial growth of B. cinerea, EC50 being 48,6 and 63,3 μL/L, respectively, followed by eucalyptol (EC50 201,6 μL/L) and ethyl butyrate (EC50 238,4 μL/L). 4-Vinyl guaiacol did not show any inhibitory effect, while the remaining tested compounds showed inhibition against B. cinerea growth, however we were not able to determine EC50 with the selected concentration ranges. Additio- naly, exposure of B. cinerea-infected grape berries to the volatiles from P. guilliermondii cultures also lowered the number of infected grape berries, when applied to in vivo assay.

Herein presented novel research approach strongly suggests that yeast produced volatiles such as ter- penes, volatile phenols and esters are one of the possible mechanisms for controlling Botrytis rot of fruit and promising biofumigants.

1. Adesida R. 2022. Exploration of yeast biodiversity potential for development of alternative biofungicides in viticulture : dissertation. University of Nova Gorica.
2. Antalick, G.; Tempère, S.; Šuklje, K.; Blackman, J.W.; Deloire, A.; Revel,, G.; Schmidtke, L. M. Investigation and Sensory Characterization of 1,4-Cineole: A Potential Aromatic Marker of Australian Cabernet Sauvignon Wine. (2015), J Agric Food Chem 63(41): 9103-11

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Article

Authors

Mitja Martelanc1, Lenart Žežlina2, Tatjana Radovanović Vukajlović1, Melita Sternad Lemut1, Lorena Butinar1

1. University of Nova Gorica, Wine Research Centre, Glavni trg 8, 5271 Vipava, Slovenia
2. University of Ljubljana, Biotechnical Faculty, Jamnikarjeva ulica 101, 1000 Ljubljana, Slovenia

Contact the author*

Keywords

volatile organic compounds, HS-SPME-GC-MS, biocontrol, Botrytis cinerea

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

Rootstock mediated responses of grapevine (Vitis vinifera L.) metabolism and physiology to combined water deficit and salinity stress in Syrah grafts

Water deficit and salinity are increasingly affecting the viticulture and wine industry. These two stresses are intimately related; understanding the physiological and metabolic responses of grapevines to water deficit, salinity and combined stress is critical for developing strategies to mitigate the nega- tive impacts of these stresses on wine grape production. These strategies can include selecting more tolerant grapevine cultivars and graft combinations, improving irrigation management, and using soil amendments to reduce the effects of salinity. For this purpose, understanding the response of grape- vine metabolism to altered water balance and salinity is of pivotal importance.

EXTRACTIBLE COMPOUNDS FROM MICROAGGLOMERATED CORK STOPPERS

After bottling, the wine continues to evolve during storage. The choice of the stopper is an important factor in this evolution. In addition to the oxygen permeability of the closure, the migration of stopper compounds into the wine can also have an impact on the wine organoleptic properties. Many studies have shown that transfers of volatile compounds from the stoppers into the wine can happen depending on the type of closure used (1). Moreover, when cork-made stoppers are used, the migration of phenolic compounds from the stopper into the wine can also occur (2, 3).

‘TROPICAL’ POLYFUNCTIONAL THIOLS AND THEIR ROLE IN AUSTRALIAN RED WINES

Following anecdotal evidence of unwanted ‘tropical’ character in red wines resulting from vineyard interventions and a subsequent yeast trial observing higher ‘red fruit’ character correlated with higher thiol concentrations, the role of polyfunctional thiols in commercial Australian red wines was investigated.
First, trials into the known tropical thiol modulation technique of foliar applications of sulfur and urea were conducted in parallel on Chardonnay and Shiraz.1 The Chardonnay wines showed expected results with elevated concentrations of 3-sulfanylhexanol (3-SH) and 3-sulfanylhexyl acetate (3-SHA), whereas the Shiraz wines lacked 3-SHA. Furthermore, the Shiraz wines were described as ‘drain’ (known as ‘reductive’ aroma character) during sensory evaluation although they did not contain thiols traditionally associated with ‘reductive’ thiols (H2S, methanethiol etc.).

TOWARDS THE SHELF-LIFE PREDICTION OF OLD CHAMPAGNE VINTAGES DEPENDING ON THE BOTTLE CAPACITY

Today, nearly one billion bottles of different sizes and capacities are aging in Champagne cellars while waiting to be put on the market. Among them, several tens of thousands of prestigious cuvees elaborated prior the 2000s are potentially concerned by prolonged aging on lees. However, when it comes to champagne tasting, dissolved CO₂ is a key compound responsible for the very much sought-after effer-vescence in glasses [1]. Yet, the slow decrease of dissolved CO₂ during prolonged aging of the most prestigious cuvees raises the issue of how long a champagne can age before it becomes unable to form CO₂ bubbles during tasting [2].

EMERGENCE OF INORGANIC PHOSPHONATE RESIDUES IN GRAPEVINE PLANT PARTS, BERRIES AND WINES FROM SOURCES OTHER THAN FOLIAR SPRAYING

Inorganic phosphonates are known to effectively support the control of grapevine downy mildew in vi- ticulture. Their application helps the plant to induce an earlier and more effective pathogen defense. However, inorganic phosphonates have been banned in organic viticulture due to their classification as plant protection products since October 2013. Despite the ban, phosphonate has been recently detected in organic wines.