terclim by ICS banner
IVES 9 IVES Conference Series 9 YEAST-PRODUCED VOLATILES IN GRAPE BASED SYSTEM MODEL ACTING AS ANTIFUNGAL BIOAGENTS AGAINST PHYTOPATHOGEN BOTRYTIS CINEREA

YEAST-PRODUCED VOLATILES IN GRAPE BASED SYSTEM MODEL ACTING AS ANTIFUNGAL BIOAGENTS AGAINST PHYTOPATHOGEN BOTRYTIS CINEREA

Abstract

Botrytis cinerea Pers., the causal agent of grey mould disease, is responsible for substantial economic losses, as it causes reduction of grape and wine quality and quantity. Exploitation of antagonistic yeasts is a promising strategy for controlling grey mould incidence and limiting the usage of synthetic fun- gicides. In our previous studies, 119 different indigenous yeasts were screened for putative multidi- mensional modes of action against filamentous fungus B. cinerea [1]. The most promissing biocontrol yeast was Pichia guilliermondii ZIM624, which exhibited several anatagonistic traits (production of cell wall degrading enzymes, chitinase and β-1,3-glucanase; demonstration of in vitro inhibitory effect on B. cinerea mycelia radial growth; production of antifungal volatiles, assimilation of a broad diversity of carbon sources, contributing to its competitivnes in inhabiting grapes in nature). In addition, P. guilliermondii ZIM 624 possessed interesting enological traits, did not produce off-flavor related H2S and appeared as β-lyase and β-glucosidase producer.

Accordingly, the aim of this researsch was to study the antifungal mechanisms by assessing the volatiles produced by P. guilliermondii ZIM624. Namely, a study was conducted to identify volatile organic com- pounds (4 higher alcohols, 6 volatile phenols, 23 esters and 27 terpenes) produced by antagonistic Pichia guilliermondii strain ZIM624 and to determine the efficacy of the chosen volatiles of P. guilliermondii in suppression of B. cinerea growth and control of Botrytis fruit rot of grape berries. Thereby, a comprehensive assessment of produced volatiles in the process of wine production was achieved using two validated analytical methods (one for terpenes and one for the rest of mentioned volatiles) comprised of automated headspace (HS) solid-phase microextraction (SPME) and gas chromatography coupled with mass spectrometric detection (GC-MS). Both methods were developed based on already published me- thod for determionation of volatiles in wine samples [2]. Among identified volatiles, 13 yeast-produced volatiles were selected and their antifungal activity was tested against B. cinerea in the fumigation bioassay. Terpenes citronellol, geraniol, nerol, α-terpineol and linalool were the most effective against B. cinerea mycelium growth with the EC50 beetwen 6,6 to 32,8 μL/L. 4-Vinyl phenol and isoamyl ace- tate also effectively inhibited mycelial growth of B. cinerea, EC50 being 48,6 and 63,3 μL/L, respectively, followed by eucalyptol (EC50 201,6 μL/L) and ethyl butyrate (EC50 238,4 μL/L). 4-Vinyl guaiacol did not show any inhibitory effect, while the remaining tested compounds showed inhibition against B. cinerea growth, however we were not able to determine EC50 with the selected concentration ranges. Additio- naly, exposure of B. cinerea-infected grape berries to the volatiles from P. guilliermondii cultures also lowered the number of infected grape berries, when applied to in vivo assay.

Herein presented novel research approach strongly suggests that yeast produced volatiles such as ter- penes, volatile phenols and esters are one of the possible mechanisms for controlling Botrytis rot of fruit and promising biofumigants.

1. Adesida R. 2022. Exploration of yeast biodiversity potential for development of alternative biofungicides in viticulture : dissertation. University of Nova Gorica.
2. Antalick, G.; Tempère, S.; Šuklje, K.; Blackman, J.W.; Deloire, A.; Revel,, G.; Schmidtke, L. M. Investigation and Sensory Characterization of 1,4-Cineole: A Potential Aromatic Marker of Australian Cabernet Sauvignon Wine. (2015), J Agric Food Chem 63(41): 9103-11

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Article

Authors

Mitja Martelanc1, Lenart Žežlina2, Tatjana Radovanović Vukajlović1, Melita Sternad Lemut1, Lorena Butinar1

1. University of Nova Gorica, Wine Research Centre, Glavni trg 8, 5271 Vipava, Slovenia
2. University of Ljubljana, Biotechnical Faculty, Jamnikarjeva ulica 101, 1000 Ljubljana, Slovenia

Contact the author*

Keywords

volatile organic compounds, HS-SPME-GC-MS, biocontrol, Botrytis cinerea

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

EFFECT OF MICRO-OXYGENATION IN COLOR OF WINES MADE WITH TOASTED VINE-SHOOTS

The use of toasted vine-shoots (SEGs) as an enological tool is a new practice that seeks to improve wines, differentiating them and encouraging sustainable wine production. The micro-oxygenation (MOX) technique is normally combined with alternative oak products with the aim to simulate the oxygen transmission rate that takes place during the traditional barrel aging. Such new use for SEGs implies a reduction in color due to the absorption by the wood of the responsible compounds, therefore, given the known effect that MOX has shown to have on the modification of wine color, its use together with the SEGs could result in an interesting implementation with the aim to obtain final wines with more stable color over time.

WHAT’S FUTURE FOR SANTORINI’S VITICULTURE IN THE CONTEXT OF CLIMATE CHANGE

The own-rooted vineyard of Santorini is a unique case of vineyard worldwide that is been cultivated for thousands of years. On the island’s volcanic soil, the vines are still cultivated with traditional techniques, which are adapted to the specific and extreme weather conditions that prevail on it. While climate change is a reality in the Mediterranean region, will Santorini vineyard endure its impact? The study of the traditional training systems, techniques and vine density, as well as the application of sustainable solutions (cover crops and use of kaolin etc.) revealed sustainable methods for the adaptation of the local viticulture to new climatic phenomena that tend to be more and more frequent in the region due to climate change.

CHANGES IN METABOLIC FLUXES UNDER LOW PH GROWTH CONDITIONS: CAN THE SLOWDOWN OF CITRATE CONSUMPTION IMPROVE OENOCOCCUS OENI ACID-TOLERANCE?

Oenococcus oeni is the main Lactic Acid Bacteria responsible for malolactic fermentation, converting malic acid into lactic acid and carbon dioxide in wines. Following the alcoholic fermentation, this second fermentation ensures a deacidification and remains essential for the release of aromatic notes and the improvement of microbial stability in many wines. Nevertheless, wine is a harsh environment for microbial growth, especially because of its low pH (between 2.9 and 3.6 depending on the type of wine) and nutrient deficiency. In order to maintain homeostasis and ensure viability, O. oeni possesses different cellular mechanisms including organic acid metabolisms which represent also the major pathway to synthetize energy in wine.

WINE CONSUMER TRADE-OFF BETWEEN ORGANOLEPTIC CHARACTERISTICS AND SUSTAINABLE CLAIMS. AN EXPERIMENT ON RED WINES FROM BORDEAUX REGION

In economics, the perception of wine quality is not limited to sensorial characteristics: an indication of the region of production significantly affects the perception of quality and consumers’ WTP ([1]; [2]). However, [3] or more recently [4] show that even if a wine has an organic label, the taste of wine remains the predominant criterion in consumer preferences. The contribution of our experiment is to evaluate the impact of responsible attributes (organic label, Non Added Sulfites, HVE certification) on the appreciation of several red wines on the market. More than 280 consumers participated to the present study and they perform 25 tastings divided into 5 different sessions. 20 different red wines from Bordeaux Area are tasted.

MODELLING THE AGEING POTENTIAL OF SYRAH RED WINES BY ACCELERATED AGEING TESTS: INFLUENCE OF ANTIOXIDANT ASSAYS AND PHENOLIC COMPOSITION

Red wine ageing is an important step in the red wine evolution and impacts its chemical and sensory characteristics through many chemicals and physico-chemical reactions. The kinetics of these evolutions depend on the wine studied and influence the wine ageing potential. Generally, high quality red wines require a longer period of bottle ageing before consumption¹. The ageing potential is an impor-tant parameter for wine quality and is related to the capacity of a wine to undergo oxidation over time². Phenolic compounds which are ones of the main substrates for oxidation can then potentially modulate ageing potential³.