terclim by ICS banner
IVES 9 IVES Conference Series 9 YEAST-PRODUCED VOLATILES IN GRAPE BASED SYSTEM MODEL ACTING AS ANTIFUNGAL BIOAGENTS AGAINST PHYTOPATHOGEN BOTRYTIS CINEREA

YEAST-PRODUCED VOLATILES IN GRAPE BASED SYSTEM MODEL ACTING AS ANTIFUNGAL BIOAGENTS AGAINST PHYTOPATHOGEN BOTRYTIS CINEREA

Abstract

Botrytis cinerea Pers., the causal agent of grey mould disease, is responsible for substantial economic losses, as it causes reduction of grape and wine quality and quantity. Exploitation of antagonistic yeasts is a promising strategy for controlling grey mould incidence and limiting the usage of synthetic fun- gicides. In our previous studies, 119 different indigenous yeasts were screened for putative multidi- mensional modes of action against filamentous fungus B. cinerea [1]. The most promissing biocontrol yeast was Pichia guilliermondii ZIM624, which exhibited several anatagonistic traits (production of cell wall degrading enzymes, chitinase and β-1,3-glucanase; demonstration of in vitro inhibitory effect on B. cinerea mycelia radial growth; production of antifungal volatiles, assimilation of a broad diversity of carbon sources, contributing to its competitivnes in inhabiting grapes in nature). In addition, P. guilliermondii ZIM 624 possessed interesting enological traits, did not produce off-flavor related H2S and appeared as β-lyase and β-glucosidase producer.

Accordingly, the aim of this researsch was to study the antifungal mechanisms by assessing the volatiles produced by P. guilliermondii ZIM624. Namely, a study was conducted to identify volatile organic com- pounds (4 higher alcohols, 6 volatile phenols, 23 esters and 27 terpenes) produced by antagonistic Pichia guilliermondii strain ZIM624 and to determine the efficacy of the chosen volatiles of P. guilliermondii in suppression of B. cinerea growth and control of Botrytis fruit rot of grape berries. Thereby, a comprehensive assessment of produced volatiles in the process of wine production was achieved using two validated analytical methods (one for terpenes and one for the rest of mentioned volatiles) comprised of automated headspace (HS) solid-phase microextraction (SPME) and gas chromatography coupled with mass spectrometric detection (GC-MS). Both methods were developed based on already published me- thod for determionation of volatiles in wine samples [2]. Among identified volatiles, 13 yeast-produced volatiles were selected and their antifungal activity was tested against B. cinerea in the fumigation bioassay. Terpenes citronellol, geraniol, nerol, α-terpineol and linalool were the most effective against B. cinerea mycelium growth with the EC50 beetwen 6,6 to 32,8 μL/L. 4-Vinyl phenol and isoamyl ace- tate also effectively inhibited mycelial growth of B. cinerea, EC50 being 48,6 and 63,3 μL/L, respectively, followed by eucalyptol (EC50 201,6 μL/L) and ethyl butyrate (EC50 238,4 μL/L). 4-Vinyl guaiacol did not show any inhibitory effect, while the remaining tested compounds showed inhibition against B. cinerea growth, however we were not able to determine EC50 with the selected concentration ranges. Additio- naly, exposure of B. cinerea-infected grape berries to the volatiles from P. guilliermondii cultures also lowered the number of infected grape berries, when applied to in vivo assay.

Herein presented novel research approach strongly suggests that yeast produced volatiles such as ter- penes, volatile phenols and esters are one of the possible mechanisms for controlling Botrytis rot of fruit and promising biofumigants.

1. Adesida R. 2022. Exploration of yeast biodiversity potential for development of alternative biofungicides in viticulture : dissertation. University of Nova Gorica.
2. Antalick, G.; Tempère, S.; Šuklje, K.; Blackman, J.W.; Deloire, A.; Revel,, G.; Schmidtke, L. M. Investigation and Sensory Characterization of 1,4-Cineole: A Potential Aromatic Marker of Australian Cabernet Sauvignon Wine. (2015), J Agric Food Chem 63(41): 9103-11

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Article

Authors

Mitja Martelanc1, Lenart Žežlina2, Tatjana Radovanović Vukajlović1, Melita Sternad Lemut1, Lorena Butinar1

1. University of Nova Gorica, Wine Research Centre, Glavni trg 8, 5271 Vipava, Slovenia
2. University of Ljubljana, Biotechnical Faculty, Jamnikarjeva ulica 101, 1000 Ljubljana, Slovenia

Contact the author*

Keywords

volatile organic compounds, HS-SPME-GC-MS, biocontrol, Botrytis cinerea

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

A NEW STRATEGY AND METHODOLOGY FOR THE CHARACTERIZATION OF POLYPHENOLS IN FINING PRECIPITATE

Polyphenols are secondary metabolite widely distributed in plant kingdom such as in fruits, in grapes and in wine. During the winemaking process, polyphenols are extract from the skin and seed of the berries. Fining is an important winemaking step just before bottling which has an impact on wine stabilization and clarification. Most the time, fining agent are animal or vegetal protein while some of them can be synthetic polymer like PVPP or natural origin like bentonite.

HOW DO ROOTSTOCKS AFFECT CABERNET SAUVIGNON AROMATIC EXPRESSION?

Grape quality potential for wine production is strongly influenced by environmental parameters such as climate and agronomic factors such as rootstock. Several studies underline the effect of rootstock on vegetative growth of the scions [1] and on berry composition [2, 3] with an impact on wine quality. Rootstocks are promising agronomic tools for climate change adaptation and in most grape-growing regions the potential diversity of rootstocks is not fully used and only a few genotypes are planted. Little is known about the effect of rootstock genetic variability on the aromatic composition in wines; thus further investigations are needed.

FUNCTIONALIZED MESOPOROUS SILICA IS A VIABLE ALTERNATIVE TO BENTONITE FOR WINE PROTEIN STABILIZATION

The presence of grape-derived heat unstable proteins can lead to haze formation in white wines [1], an instability prevented by removing these proteins by adding bentonite, a hydrated aluminum silicate that interacts electrostatically with wine proteins leading to their flocculation. Despite effective, using bentonite has several drawbacks as the costs associated with its use, the potential negative effects on wine quality, and its environmental impact, so that alternative solutions are needed.

WINE CONSUMER TRADE-OFF BETWEEN ORGANOLEPTIC CHARACTERISTICS AND SUSTAINABLE CLAIMS. AN EXPERIMENT ON RED WINES FROM BORDEAUX REGION

In economics, the perception of wine quality is not limited to sensorial characteristics: an indication of the region of production significantly affects the perception of quality and consumers’ WTP ([1]; [2]). However, [3] or more recently [4] show that even if a wine has an organic label, the taste of wine remains the predominant criterion in consumer preferences. The contribution of our experiment is to evaluate the impact of responsible attributes (organic label, Non Added Sulfites, HVE certification) on the appreciation of several red wines on the market. More than 280 consumers participated to the present study and they perform 25 tastings divided into 5 different sessions. 20 different red wines from Bordeaux Area are tasted.

OPTIMIZATION, VALIDATION AND APPLICATION OF THE EPR SPIN-TRAPPING TECHNIQUE TO THE DETECTION OF FREE RADICALS IN CHARDONNAY WINES

The aging potential of Burgundy chardonnay wines is considered as quality indicator. However, some of them exhibit higher oxidative sensitivity and premature oxidative aging symptoms, which are potentially induced by no-enzymatic oxidation such as Fenton-type reaction (Danilewicz, 2003). This chemical mechanism involves the action of transition metal, native phenolic compounds and oxygen which promote the generation of highly reactive oxygen species (ROS) such as hydroxyl radicals (OH) or 1-hydroxyethyl radicals (1-HER) from oxidation of ethanol. Such mechanism is involved in the radical oxidation occurring during bottle aging. According to Elias et al.,(2009a), the 1-HER is the most abundant radical in forced oxidation treated wines. Consequently, understanding its evolution kinetic in dry white wines is of great importance.