terclim by ICS banner
IVES 9 IVES Conference Series 9 ANALYZING THE ROLE OF ELEMENTAL SULFUR IN GRAPE JUICE ON THE DEVELOPMENT OF POLYFUNCTIONAL MERCAPTANS IN SAUVIGNON BLANC WINES

ANALYZING THE ROLE OF ELEMENTAL SULFUR IN GRAPE JUICE ON THE DEVELOPMENT OF POLYFUNCTIONAL MERCAPTANS IN SAUVIGNON BLANC WINES

Abstract

Sauvignon blanc is characterized by distinctive aromas, both fruity and herbaceous. The “green” cha- racter has been attributed to the methoxypyrazines, while the “fruity” character is associated with polyfunctional mercaptans . Polyfunctional mercaptans are of great significance due to their high impact on wines and associated low perception thresholds.

Elemental sulfur (S⁰) is widely used to protect grapevines from powdery mildew. Machine harvesting can enhance the formation of C6-compounds from grape lipids, leading to desirable polyfunctional mer- captan formation in wines.

A missing piece of information in most past studies on the formation of polyfunctional thiols has been the concentration of elemental sulfur in grapes. In this research, we aimed first to develop an easy and applicable method for a winery setting to analyse elemental sulfur concentration in grape juice samples. With this method in place, trials were then established to examine the link between elemental sulfur in the juice and 3MH/3MHA formation in wines. The trials were undertaken during three consecutive harvests in New Zealand in 2020, 2021, and 2022.

The study developed a sulfide sensor to measure elemental sulfur levels in grape juice samples and in- vestigated the correlation between S⁰ and polyfunctional mercaptan concentration in resulting wines. We reduced S⁰ to sulfide using dithiothreitol in acidic conditions and used an ion-selective electrode to measure sulfide concentrations. GC-MS was used to compare thiol concentration in wine with juice S⁰ levels from 2020 and 2021 samples. The investigation was expanded in 2022 by manually applying S⁰ to grapes at various intervals prior to harvesting and analyzing the relationship between residual S⁰ levels in juice and polyfunctional mercaptans in resulting wines.

The study established a dependable method based on ion-selective analysis and produced accurate ca- libration curves. The reduction process was found to be effective and the apparatus performed well with both standard and juice samples. Additionally, the results from the 2020 and 2021 trials revealed a cor- relation between increased juice elemental sulfur and a higher formation of 3MH/3MHA, supporting the theory that S⁰ contributes to the formation of 3MH in wine. This correlation was further confirmed in the 2022 trial, which saw a substantial increase in 3MH/3MHA in wines resulting from the manual application of S⁰ to the grapes through late spraying in the field.

 

1. Lund, C. M.; Thompson, M. K.; Benkwitz, F.; Wohler, M. W.; Triggs, C. M.; Gardner, R.; Heymann, H.; Nicolau, L. American Jour-nal of Enology and Viticulture 2009, 60, 1.
2. Harsch, M. J.; Benkwitz, F.; Frost, A.; Colonna-Ceccaldi, B.; Gardner, R. C.; Salmon, J.-M. Journal of agricultural and food che-mistry 2013, 61, 3703-3713.
3. Kwasniewski, M.T.; Sacks, G.L.; Wilcox, W.F. J. Enol. Vitic. 2014, 65, 453-462.
4. Lyu, X.; Dias Araujo, L.; Quek, S.-Y.; Kilmartin, P. A. Food Chemistry 2021, 346, 128914.
5. Araujo, L. D.; Vannevel, S.; Buica, A.; Callerot, S.; Fedrizzi, B.; Kilmartin, P. A.; du Toit, W. J. Food Research International 2017, 98, 79-86.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Article

Authors

Bahareh Sarmadi¹, Paul A. Kilmartin¹, Leandro D. Araújo ², Brandt P. Bastow¹

1. School of Chemical Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand
2. Department of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln, New Zealand 

Contact the author*

Keywords

Sauvignon blanc, polyfunctional mercaptans, elemental sulfur, varietal thiols

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

AN AUTOMATIC CANOPY COOLING SYSTEM TO COPE WITH THE THERMAL-RADIATIVE STRESSES IN THE PIGNOLETTO WHITE GRAPE

In recent years characterized by hot dry summers, the implementation of innovative irrigation tools in the vineyard represents a crucial challenge to ensure optimal production and to avoid excess of water consumption. It is known that the grapevine reacts to multiple stresses – i.e., high temperatures and wa- ter shortage – through adaptive mechanisms that are detrimental to the yield. Furthermore, this condi- tion is usually aggravated by high solar radiation, which could negatively affect the phenolic composi- tion of the grapes. Therefore, a cooling system has been developed aiming to reduce bunches’ sunburn damage.

FLOW CYTOMETRY, A POWERFUL AND SUSTAINABLE METHOD WITH MULTIPLE APPLICATIONS IN ENOLOGY

Flow cytometry (FCM) is a powerful technique allowing the detection, characterization and quantification of microbial populations in different fields of application (medical environment, food industry, enology, etc.). Depending on the fluorescent markers and specific probes used, FCM provides information on the physiological state of the cell and allows the quantification of a microorganism of interest within a mixed population. For 15 years, the enological sector has shown growing interest in this technique, which is now used to determine the populations present (of interest or spoilage) and the physiological state of microorganisms at the different stages of winemaking.

NEW INSIGHTS INTO VOLATILE SULPHUR COMPOUNDS SCALPING ON MICROAGGLOMERATED WINE CLOSURES

The evolution of wine during bottle ageing has been of great interest to ensure consistent quality over time. While the role of wine closures on the amount of oxygen is well-known [1], closures could also play other roles such as the scalping phenomenon of flavour compounds. Flavour scalping has been described as the sorption of flavour compounds by the packaging material, which could result in losses of flavour intensity. It has been reported in the literature that volatile sulphur compounds (VSC) can be scalped on wine closures depending on the type of closure (traditional and agglomerated cork, screw-cap, synthetic [2]).

THE EFFECT OF DIFFERENT TERROIRS ON AROMA COMPOUNDS OF ‘KALECIK KARASI’ WINES

Kalecik Karası is a domestic grape variety of Turkey, originating from Kalecik district, 80 km from Ankara. Although there is no definite evidence, it is known that it was used in wine production by many civilizations that lived in the Anatolian region, especially the Hittites. Compared to other black wine grapes, it stands out with its low tannin content, rich fruity aroma and complex structure. In good vintages, red fruits such as strawberries, cherries and raspberries stand out in the aroma profile. Although its structure is elegant, it has the potential to age and develop similar to the ‘Pinot Noir’ wine of the Burgundy region. This offers a complex aroma structure including red flowers, earth and ripe fruits.

Searching for the sweet spot: a focus on wine dealcoholization

It is well known that the vinification of grapes at full maturation can produce rich, full-bodied wines,
with intense and complex flavour profiles. However, the juice obtained from such grapes may have very
high sugar concentration, resulting in wines with an excessive concentration of ethanol. In addition, the decoupling between technological maturity and phenolic/aromatic one due to global warming, exacerbates this problem in some wine-growing regions. In parallel with the increase of the mean alcohol content of wines on the market, also the demand for reduced alcohol beverages has increased in recent years, mainly as a result of health and social concerns about the risks related to the consumption of alcohol.