terclim by ICS banner
IVES 9 IVES Conference Series 9 IMPACT OF GRAPE-ASSOCIATED MOLDS IN FRESH MUSHROOM AROMA PRODUCTION

IMPACT OF GRAPE-ASSOCIATED MOLDS IN FRESH MUSHROOM AROMA PRODUCTION

Abstract

Mycobiota encountered from vine to wine is a complex and diversified ecosystem that may impact grape quality at harvest and the sensorial properties of wines, thus leading to off-flavors [1-3]. Among known off-flavors in wine, fresh mushroom aroma (FMA) has been linked to some mold species, naturally pre-sent on grapes, producing specific volatile organic compounds (VOC) [4-5]. The most well-known are 1-octen-3-ol and 1-octen-3-one, although many other VOC are likely involved. To better understand the FMA defect, biotic and abiotic factors impacting growth kinetics and VOC production of selected fungal species in must media and on grapes were studied. In total, 18 fungal isolates, belonging to 7 genera and 9 species, were selected based on their off-odor production profiles on malt medium among 685 isolates from our working collection. Growth rates were measured using solid synthetic must (MS) and real must (MR) and compared to those obtained in liquid must by laser nephelometry. Sensorial analysis and VOC profiles (GC-MS) were also determined for the same isolates, individually or in co-cultures with two FMA producing Botrytis isolates, after growth on must and grapes. Among the generated physiological data, optimal growth temperatures were 27-28°C, 26-30°C, 21-22°C for Botrytis spp., Penicillium crocicola and P. citreonigrum, respectively, depending on the isolate. Fastest growth rates were observed for B. cinerea and P. crocicola, while Cladosporium subtilissimum and P. brevicompactum isolates were slowest. For VOC profiles, P. crocicola, P. bialowiezense and Clonostachys rosea produced known FMA compounds (1-octen-3-one and 1-octen-3-ol) at higher levels when co-inoculated with Botrytis spp. on grapes. For must trials, a species effect on VOC profiles was clearly observed (92 VOC identified). To confirm these findings, further co-inoculation studies were performed on two grape varieties (Meunier and Pinot noir) and, so far, sensorial analyses showed similar trends. Overall, this study provides novel knowledge about changes in fungal growth kinetics and VOC profiles in musts and on grapes. These results provide new insights for the wine making to better understand how FMA off-flavors are generated by molds.

 

1. Scott et al, 2022 doi.org/10.1016/B978-0-08-102067-8.00006-3
2. Steel et al, 2013 doi.org/10.1021/jf400641r
3. Rousseaux et al 2014 doi:10.1016/j.fm.2013.08.013
4. La Guerche et al, 2006 doi.org/10.1016/B978-0-08-102067-8.00006-3
5. Meistermann et al, 2020 (DOI:10.20870/oeno-one.2021.55.3.3004)

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Adrien Destanque1,2, Alexis Commereuc1, Flora Pensec1, Adeline Picot1, Anne Thierry3, Marie-Bernadette Maillard3, Louis Corol-ler, Sylvie Treguer-Fernandez1, Emmanuel Coton1, Marion Hervé2 and Monika Coton1*

1. Univ Brest, INRAE, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, F-29280 Plouzané, France
2. Centre de Recherche Robert-Jean de Vogüé Moët Hennessy, F-51530 Oiry, France
3. INRAE, Institut Agro, UMR STLO, F-35000 Rennes, France

Contact the author*

Keywords

Mycobiot, growth modeling, volatile organic compounds (VOCs)

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

THE EFFECT OF COPPER ON THE PRODUCTION OF VARIETAL THIOLS DURING THE ALCOHOLIC FERMENTATION OF COLOMBARD AND GROS MANSENG GRAPE JUICES

Nowadays, the rapid growth of vineyards with organic practices and the use of copper as the only fun-gicide against downy mildew raises again the question of the effect of copper on varietal thiols in wine, especially 3-sulfanylhexan-1-ol (3SH) and its acetate (3SHA). A few decades ago, several works indicated that the use of copper in the vineyard had a negative effect on the content of varietal thiols in Sauvignon blanc wines [1, 2]. However, these studies only considered the concentration of the reduced form (RSH) of varietal thiols, without quantifying the oxidised ones. For this purpose, we proposed to monitor both reduced and oxidised forms of varietal thiols in wine under copper stress during alcoholic fermentation to have a more complete picture of the biological and chemical mechanisms.

EFFECTIVENESS OF APPLIED MATERIALS IN REDUCING THE ABSORPTION OF SMOKE MARKER COMPOUNDS IN A SIMULATED WILDFIRE SCENARIO

Smoke taint (ST) is a grape-wine off-flavour that may occur when grapes absorb volatile phenols (VPs) originating from wildfire smoke (1). ST is associated with the negative sensory attributes such as smoky and ashy notes. VPs are glycosylated in the plant and thus present in both free and bound forms (2; 3). Wildfire smoke has resulted in a decline in grape and wine quality and financial losses which has become a prominent issue for the global wine industry.

MOVING FROM SULFITES TO BIOPROTECTION: WHICH IMPACT ON CHARDONNAY WINE?

Over the last few years, several tools have been developed to reduce the quantity of sulfites used during winemaking, including bioprotection. Although its effectiveness in preventing the development of spoilage microorganisms has been proven, few data are available on the impact of sulfite substitution by bioprotection on the final product. The objective of this study was therefore to characterize Chardonnay wines with the addition of sulfite or bioprotection in the pre-fermentation stage. The effects of both treatments on resulting matrices was evaluated at several scales: analysis of classical oenological parameters, antioxidant capacity, phenolic compounds, non-volatile metabolome and sensory profile.

GRAPE SPIRITS FOR PORT WINE PRODUCTION: SCREENING THEIR AROMA PROFILE

Port is a fortified wine, produced from grapes grown in the demarcated Douro region. The fortification process consists in the addition of a grape spirit (77% v/v) to the fermenting juice for fermentation interruption, resulting in remaining residual sugars in the wine and increased alcohol content (19-22%). The approval of grape spirits follows the Appellation (D.O. Port wine) rules1 and it is currently carried out based on analytical control and on sensory evaluation done by the public Institute that upholds the control of the quality of Douro Appellation wines. However, the producers of Port wines would like to have more information about quality markers of grape spirits.

INFLUENCE OF THE NITROGEN / LIPIDS RATIO OF MUSTS ON THE REVELATION OF AROMATIC COMPOUNDS IN SAUVIGNON BLANC WINE

Production of volatile compounds by yeast is known to be modulated by must nitrogen. Nevertheless, various parameter of must quality have an impact on yeast fermentation. In this study we propose to evaluate the impact of nitrogen / lipids balance on a Sauvignon Blanc grape juice (Val de Loire).
Must was prepared from the same grapes at pilot scale. Three modalities were carried out: direct pressing, direct pressing with a pre-fermentation cold stabulation and pellicular maceration before pressing.