terclim by ICS banner
IVES 9 IVES Conference Series 9 IMPACT OF GRAPE-ASSOCIATED MOLDS IN FRESH MUSHROOM AROMA PRODUCTION

IMPACT OF GRAPE-ASSOCIATED MOLDS IN FRESH MUSHROOM AROMA PRODUCTION

Abstract

Mycobiota encountered from vine to wine is a complex and diversified ecosystem that may impact grape quality at harvest and the sensorial properties of wines, thus leading to off-flavors [1-3]. Among known off-flavors in wine, fresh mushroom aroma (FMA) has been linked to some mold species, naturally pre-sent on grapes, producing specific volatile organic compounds (VOC) [4-5]. The most well-known are 1-octen-3-ol and 1-octen-3-one, although many other VOC are likely involved. To better understand the FMA defect, biotic and abiotic factors impacting growth kinetics and VOC production of selected fungal species in must media and on grapes were studied. In total, 18 fungal isolates, belonging to 7 genera and 9 species, were selected based on their off-odor production profiles on malt medium among 685 isolates from our working collection. Growth rates were measured using solid synthetic must (MS) and real must (MR) and compared to those obtained in liquid must by laser nephelometry. Sensorial analysis and VOC profiles (GC-MS) were also determined for the same isolates, individually or in co-cultures with two FMA producing Botrytis isolates, after growth on must and grapes. Among the generated physiological data, optimal growth temperatures were 27-28°C, 26-30°C, 21-22°C for Botrytis spp., Penicillium crocicola and P. citreonigrum, respectively, depending on the isolate. Fastest growth rates were observed for B. cinerea and P. crocicola, while Cladosporium subtilissimum and P. brevicompactum isolates were slowest. For VOC profiles, P. crocicola, P. bialowiezense and Clonostachys rosea produced known FMA compounds (1-octen-3-one and 1-octen-3-ol) at higher levels when co-inoculated with Botrytis spp. on grapes. For must trials, a species effect on VOC profiles was clearly observed (92 VOC identified). To confirm these findings, further co-inoculation studies were performed on two grape varieties (Meunier and Pinot noir) and, so far, sensorial analyses showed similar trends. Overall, this study provides novel knowledge about changes in fungal growth kinetics and VOC profiles in musts and on grapes. These results provide new insights for the wine making to better understand how FMA off-flavors are generated by molds.

 

1. Scott et al, 2022 doi.org/10.1016/B978-0-08-102067-8.00006-3
2. Steel et al, 2013 doi.org/10.1021/jf400641r
3. Rousseaux et al 2014 doi:10.1016/j.fm.2013.08.013
4. La Guerche et al, 2006 doi.org/10.1016/B978-0-08-102067-8.00006-3
5. Meistermann et al, 2020 (DOI:10.20870/oeno-one.2021.55.3.3004)

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Adrien Destanque1,2, Alexis Commereuc1, Flora Pensec1, Adeline Picot1, Anne Thierry3, Marie-Bernadette Maillard3, Louis Corol-ler, Sylvie Treguer-Fernandez1, Emmanuel Coton1, Marion Hervé2 and Monika Coton1*

1. Univ Brest, INRAE, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, F-29280 Plouzané, France
2. Centre de Recherche Robert-Jean de Vogüé Moët Hennessy, F-51530 Oiry, France
3. INRAE, Institut Agro, UMR STLO, F-35000 Rennes, France

Contact the author*

Keywords

Mycobiot, growth modeling, volatile organic compounds (VOCs)

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

INVESTIGATION OF MALIC ACID METABOLIC PATHWAYS DURING ALCOHOLIC FERMENTATION USING GC-MS, LC-MS, AND NMR DERIVED 13C-LABELED DATA

Malic acid has a strong impact on wine pH and the contribution of fermenting yeasts to modulate its concentration has been intensively investigated in the past. Recent advances in yeast genetics have shed light on the unexpected property of some strains to produce large amounts of malic acid (“acidic strains”) while most of the wine starters consume it during the alcoholic fermentation. Being a key metabolite of the central carbohydrate metabolism, malic acid participates to TCA and glyoxylate cycles as well as neoglucogenesis. Although present at important concentrations in grape juice, the metabolic fate of malic acid has been poorly investigated.

NEUROPROTECTIVE AND ANTI-INFLAMMATORY PROPERTIES OF HYDROXYTYROSOL: A PROMISING BIOACTIVE COMPONENT OF WINE

Hydroxytyrosol (HT) is a phenolic compound present in olives, virgin olive oil and wine. HT has attracted great scientific interest due to its biological activities which have been related with the ortho-dihydroxy conformation in the aromatic ring. In white and red wines, HT has been detected at concentrations ranging from 0.28 to 9.6 mg/L and its occurrence has been closely related with yeast metabolism of aromatic amino acids by Ehrlich pathway during alcoholic fermentation. One of the most promising properties of this compound is the neuroprotective activity against pathological mechanisms related with neurode-generative disorders including Alzheimer’s and Parkinson’s disease.

SENSORY IMPROVEMENT OF DEALCOHOLISED WINES

Interest and willing-ness to buy alcohol-free wines by customers is increasing for several years [1]. Due to the rising relevance of dealcoholised wines it is the objective of this study to contribute to a better understanding of the flavor variation among dealcoholised wines and to explore enological measures, how to improve final quality.
First a range of commercial, alcoholfree white wines were analysed by the holistic sensory method projective mapping, including a question for hedonic acceptance. Based on the combination of a non-target-HS-SPME-GC/MS analysis with sensory analysis we obtained a clustering of the wines into three groups.

EXPLORING THE METABOLIC AND PHENOTYPIC DIVERSITY OF INDIGENOUS YEASTS ISOLATED FROM GREEK WINE

Climate change leads to even more hostile and stressful for the wine microorganism conditions and consequently issues with fermentation rate progression and off-character formation are frequently observed. The objective of the current research was to classify a great collection of yeast isolates from Greek wines based on their technological properties with oenological interest. Towards this direction, fourteen spontaneously fermented wines from different regions of Greece were collected for further yeast typing. The yeast isolates were subjected in molecular analyses and identification at species level.

VALORIZATION OF GRAPE WINE POMACE USING PULSED ELECTRIC FIELDS (PEF) AND SUPERCRITICAL CO₂ (SC CO₂) EXTRACTION

Wine grape pomace quantitatively and qualitatively represents the most important fraction of wine waste. Namely, this by-product makes ~ 20% of the total mass of vinified grapes, and it is characterized with high concentrations of polyphenolic antioxidants, as well as grape seed oil. Hence, valorization of wine pomace, as an alternative to traditionally employed disposal, has drown considerable interest in recent years. Earlier studies were mostly focused on the extraction of phenolics, while mechanisms enhancing the extraction of lipid fraction from grape pomace, as well as their impact on the grape seed oil quality are far less investigated.