terclim by ICS banner
IVES 9 IVES Conference Series 9 IMPACT OF GRAPE-ASSOCIATED MOLDS IN FRESH MUSHROOM AROMA PRODUCTION

IMPACT OF GRAPE-ASSOCIATED MOLDS IN FRESH MUSHROOM AROMA PRODUCTION

Abstract

Mycobiota encountered from vine to wine is a complex and diversified ecosystem that may impact grape quality at harvest and the sensorial properties of wines, thus leading to off-flavors [1-3]. Among known off-flavors in wine, fresh mushroom aroma (FMA) has been linked to some mold species, naturally pre-sent on grapes, producing specific volatile organic compounds (VOC) [4-5]. The most well-known are 1-octen-3-ol and 1-octen-3-one, although many other VOC are likely involved. To better understand the FMA defect, biotic and abiotic factors impacting growth kinetics and VOC production of selected fungal species in must media and on grapes were studied. In total, 18 fungal isolates, belonging to 7 genera and 9 species, were selected based on their off-odor production profiles on malt medium among 685 isolates from our working collection. Growth rates were measured using solid synthetic must (MS) and real must (MR) and compared to those obtained in liquid must by laser nephelometry. Sensorial analysis and VOC profiles (GC-MS) were also determined for the same isolates, individually or in co-cultures with two FMA producing Botrytis isolates, after growth on must and grapes. Among the generated physiological data, optimal growth temperatures were 27-28°C, 26-30°C, 21-22°C for Botrytis spp., Penicillium crocicola and P. citreonigrum, respectively, depending on the isolate. Fastest growth rates were observed for B. cinerea and P. crocicola, while Cladosporium subtilissimum and P. brevicompactum isolates were slowest. For VOC profiles, P. crocicola, P. bialowiezense and Clonostachys rosea produced known FMA compounds (1-octen-3-one and 1-octen-3-ol) at higher levels when co-inoculated with Botrytis spp. on grapes. For must trials, a species effect on VOC profiles was clearly observed (92 VOC identified). To confirm these findings, further co-inoculation studies were performed on two grape varieties (Meunier and Pinot noir) and, so far, sensorial analyses showed similar trends. Overall, this study provides novel knowledge about changes in fungal growth kinetics and VOC profiles in musts and on grapes. These results provide new insights for the wine making to better understand how FMA off-flavors are generated by molds.

 

1. Scott et al, 2022 doi.org/10.1016/B978-0-08-102067-8.00006-3
2. Steel et al, 2013 doi.org/10.1021/jf400641r
3. Rousseaux et al 2014 doi:10.1016/j.fm.2013.08.013
4. La Guerche et al, 2006 doi.org/10.1016/B978-0-08-102067-8.00006-3
5. Meistermann et al, 2020 (DOI:10.20870/oeno-one.2021.55.3.3004)

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Adrien Destanque1,2, Alexis Commereuc1, Flora Pensec1, Adeline Picot1, Anne Thierry3, Marie-Bernadette Maillard3, Louis Corol-ler, Sylvie Treguer-Fernandez1, Emmanuel Coton1, Marion Hervé2 and Monika Coton1*

1. Univ Brest, INRAE, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, F-29280 Plouzané, France
2. Centre de Recherche Robert-Jean de Vogüé Moët Hennessy, F-51530 Oiry, France
3. INRAE, Institut Agro, UMR STLO, F-35000 Rennes, France

Contact the author*

Keywords

Mycobiot, growth modeling, volatile organic compounds (VOCs)

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

AROMATIC AND FERMENTATIVE PERFORMANCES OF HANSENIASPORA VINEAE IN DIFFERENT SEQUENTIAL INOCULATION PROTOCOLS WITH SACCHAROMYCES CEREVISIAE FOR WHITE WINEMAKING

Hanseniaspora vineae (Hv) is a fermenting non-Saccharomyces yeast that compared to Saccharomyces cerevisiae (Sc) present some peculiar features on its metabolism that make it attractive for its use in wine production. Among them, it has been reported a faster yeast lysis and release of polysaccharides, as well as increased ß-glucosidase activity. Hv also produces distinctive aroma compounds, including elevated levels of fermentative compounds such as ß-phenylethyl acetate and norisoprenoids like safranal. However, it is known for its high nutritional requirements, resulting in prolonged and sluggish fermentations, even when complemented with Sc strain and nutrients.

TARTARIC STABILIZATION MAY AFFECT THE COLOR AND POLYPHENOLIC COMPOSITION OF TANNAT RED WINES FROM URUGUAY

Tartrate precipitation affects the properties of wines, due to the formation of crystals that cause turbidity, even after being bottled. The forced tartaric stabilization is carried out frequently for young wines, through various physicochemical procedures. The traditional treatment for tartaric stabilization is refrigeration, but it can have a negative effect on wine’s sensory properties, and particularly on the color of red wines. The aim of this study was to evaluate the effect of different tartaric stabilization options on the color and phenolic composition of Tannat red wines from Uruguay.

VOLATILE COMPOSITION OF WINES USING A GC/TOFMS: HS-SPME VS MICRO LLE AS SAMPLE PREPARATION METHODOLOGY

Wine aroma analysis can be done by sensorial or instrumental analysis, the latter involving several me-thodologies based on olfactometric detection, electronic noses or gas chromatography. Gas Chromatography has been widely used for the study of the volatile composition of wines and depending on the detection system coupled to the chromatographic system, quantification and identification of individual compounds can be achieved.

AROMA AND SENSORY CHARACTERIZATION OF XINOMAVRO RED WINES FROM DIFFERENT GREEK PROTECTED DESIGNATIONS OF ORIGIN, EFFECT OF TERROIR CHARACTERISTICS

The quality of wines has often been associated with their geographical area of production. The aim of this work was to characterize Protected Designation of Origin (PDO) Xinomavro red wines from different geographical areas of Amyndeon and Naoussa in Northern Greece, elaborated with variables that contribute to their differentiation, such as soil characteristics, altitude, monthly average temperature and rainfall.
Xinomavro fruit parcels from different vineyards within the two PDO zones (5 PDO Naoussa and 6 PDO Amyndeon) were vinified following a standard winemaking process. A total of 25 aroma compounds were quantified using gas chromatography-mass spectrometry (GC-MS) with simultaneous full scan and selected ion monitoring for data recording, and odor activity values (OAVs) were determined.

ENRICHMENT OF THE OENOLOGICAL MALDI-TOF/MS PROTEIN SPECTRA DATABASE FOR RELIABLE OENOLOGICAL YEAST AND BACTERIA IDENTIFICATION

The Matrix Assisted Laser Desorption/Ionization–Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) technology is commonly used in food and medical sector to identify yeast or bacteria species isolated from a nutritive culture media. Since a decade, brewery and oenology industries have been attracted to this method which combines fast analysis times, reliability and low cost of analysis. Briefly, this method is based on the comparison of the MALDI-TOF/MS protein spectra of an isolated colony of yeast or bacteria with those contain in a manufacturer’s reference protein spectra database. Initiated in 2015, the creation of the first oenological mass spectra database has proved to be essential for increase quality of species identification.