terclim by ICS banner
IVES 9 IVES Conference Series 9 THE EFFECT OF COPPER ON THE PRODUCTION OF VARIETAL THIOLS DURING THE ALCOHOLIC FERMENTATION OF COLOMBARD AND GROS MANSENG GRAPE JUICES

THE EFFECT OF COPPER ON THE PRODUCTION OF VARIETAL THIOLS DURING THE ALCOHOLIC FERMENTATION OF COLOMBARD AND GROS MANSENG GRAPE JUICES

Abstract

Nowadays, the rapid growth of vineyards with organic practices and the use of copper as the only fungicide against downy mildew raises again the question of the effect of copper on varietal thiols in wine, especially 3-sulfanylhexan-1-ol (3SH) and its acetate (3SHA). A few decades ago, several works indicated that the use of copper in the vineyard had a negative effect on the content of varietal thiols in Sauvignon blanc wines [1, 2]. However, these studies only considered the concentration of the reduced form (RSH) of varietal thiols, without quantifying the oxidised ones. For this purpose, we proposed to monitor both reduced and oxidised forms of varietal thiols in wine under copper stress during alcoholic fermentation to have a more complete picture of the biological and chemical mechanisms. In the present work, Colombard and Gros Manseng grape juices were fermented under different copper levels (from 0.2 to 3.88 mg/L) to mimic the consequences of organic practices on grape and must. The consumption of thiol precursors and the release of varietal thiols (both free and oxidised forms of 3SH and 3SHA) were monitored by LC-MS/MS according to previously published methods [3, 4]. It was found that the highest copper content (3.6 and 3.88 mg/L for Colombard and Gros Manseng, respectively) significantly increased yeast consumption of precursors (by 9.0 and 7.6% for Colombard and Gros Manseng, respectively). Surprisingly, this higher consumption of precursors was not associated to higher thiol concentrations. Indeed, for both varieties, the content of free thiols in the wine decreased significantly (by 84 and 47% for Colombard and Gros Manseng, respectively) with the increase of copper in the starting must, as already described in the literature [1, 2]. However, the sum “reduced+oxidized” forms of 3SH produced during fermentation was constant for the Colombard must regardless of the copper conditions, which means that the effect of copper was only oxidative for this variety. In Gros Manseng, on the other hand, the sum “reduced+oxidized” forms of 3SH increased with the copper content, up to 90%. This last result suggests that copper probably modifies the regulation of the production pathways of varietal thiols and has also a key role of oxidation. These results complement our knowledge on the effect of copper during thiol-oriented fermentation and the importance of considering both “reduced+oxidized” forms to distinguish chemical from biological effects.

 

1. Darriet, P., et al., Effects of copper fungicide spraying on volatile thiols of the varietal aroma of Sauvignon blanc, Cabernet Sauvignon and Merlot wines. VITIS-GEILWEILERHOF-, 2001. 40(2): p. 93-100.
2. Hatzidimitriou, E., et al., Incidence d’une protection viticole anticryptogamique utilisant une formulation cuprique sur le niveau de maturité des raisins et l’arôme variétal des vins de Sauvignon:(Bilan de trois années d’expérimentation). Journal International des Sciences de la Vigne et du Vin, 1996. 30(3): p. 133-150.
3. Roland, A., et al., Innovative analysis of 3-mercaptohexan-1-ol, 3-mercaptohexylacetate and their corresponding disulfides in wine by Stable Isotope Dilution Assay and nano-liquid chromatography tandem mass spectrometry. Journal of Chromatography A, 2016. 1468: p. 154-163.
4. Bonnaffoux, H., et al., First identification and quantification of S-3-(hexan-1-ol)-γ-glutamyl-cysteine in grape must as a potential thiol precursor, using UPLC-MS/MS analysis and stable isotope dilution assay. Food Chemistry, 2017. 237: p. 877-886

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

G. Dournes1, T. Dufourcq², L. Suc1, J.-R. Mouret1 and A. Roland1*

1. SPO, Université de Montpellier, INRAE, Institut Agro, Montpellier, France
2. Institut Français de la Vigne et du Vin, Pôle Sud-Ouest, Caussens, France

Contact the author*

Keywords

3-sulfanylhexan-1-ol, copper, alcoholic fermentation, yeast

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

ALCOHOLIC FERMENTATION AND COLOR OF ROSÉ WINES: INVESTIGATIONS ON THE MECHANISMS RESPONSIBLE FOR SUCH DIVERSITY

Color is one of the key elements for the marketing of rosé wines due to their packaging in transparent bottles. Their broad color range is due to the presence of pigments belonging to phenolic compounds extracted from grapes or formed during the wine-making process. However, the mechanisms responsible for such diversity are poorly understood. The few investigations performed on rosé wines showed that their phenolic composition is highly variable, close to that of red wines for the darkest rosés but very different for light ones [1]. Moreover, large variations in the extent of color loss taking place during fermentation have been reported but the mechanisms involved and causes of such variability are unknown.

INTENSE PULSED LIGHT FOR VINEYARD WASTEWATER: A PROMISING NEW PROCESS OF DEGRADATION FOR PESTICIDES

The use of pesticides for vine growing is responsible for generating an important volume of wastewater. In 2009, 13 processes were authorized for wastewater treatment but they are expensive and the toxicological impact of the secondary metabolites that are formed is not clearly established. Recently photodecomposition processes have been studied and proved an effectiveness to degrade pesticides and to modify their structures (Maheswari et al., 2010, Lassale et al., 2014). In this field, Pulsed Light (PL) seems to be an interesting and efficient process (Baranda et al., 2017). Therefore, the aim of this work was to investigate the PL technology as a new process for the degradation of pesticides.

Grouping Vitis vinifera grapevine varieties based on their aromatic composition

Climate change is likely to impact wine typicity across the globe, raising concerns in wine regions historically renowned for the quality of their terroir1. Amongst several changes in viticultural practices, replacing some of the planting material (i.e. clones, rootstocks and cultivars) is thought to be one of the most promising potential levers to be used for adapting to climate change. But the change of cultivars also involves the issue of protecting the region’s wine typicity. In Bordeaux (France), extensive research has been conducted on identifying meridional varieties that could be good candidates to help guard against the effects of climate change2 while less research has been done concerning their impacts on Bordeaux wine typicity.

EXTRACTIBLE COMPOUNDS FROM MICROAGGLOMERATED CORK STOPPERS

After bottling, the wine continues to evolve during storage. The choice of the stopper is an important factor in this evolution. In addition to the oxygen permeability of the closure, the migration of stopper compounds into the wine can also have an impact on the wine organoleptic properties. Many studies have shown that transfers of volatile compounds from the stoppers into the wine can happen depending on the type of closure used (1). Moreover, when cork-made stoppers are used, the migration of phenolic compounds from the stopper into the wine can also occur (2, 3).

HOW DOES ULTRASOUND TREATMENT AFFECT THE AGEING PROFILE OF AN ITALIAN RED WINE?

Many wine styles require moderate or extended ageing to ensure optimal consumer experience. However, few consumers have the interest or ability to age wine themselves, and holding wine in optimal conditions for extended periods is expensive for producers. A study was conducted on the use of ul-trasound energy on wine, with particular reference to its impact on sensory and chemical profiles. The OIV has authorised the use of ultrasound for processing crushed grapes (must) in Resolution OENO 616-2019, but not yet for finished wine1,2.