OENO IVAS 2019 banner
IVES 9 IVES Conference Series 9 OENO IVAS 9 OENO IVAS 2019 9 Chemical and Biochemical reactions, including grape and wines microorganisms impact 9 Oenological tannins to prevent Botrytis cinerea damage: reduction of laccase activity

Oenological tannins to prevent Botrytis cinerea damage: reduction of laccase activity

Abstract

Oenological tannins are classified as hydrolysable and condensed tannins. Their use in winemaking is only authorized, to facilitate wine fining. Nevertheless, tannins could also be used to prevent laccase effects. 

Indeed, our group has recently proved their effects against laccase damage [1]. The goal of this study was to better understand the mechanism of action of oenological tannins on laccase activity induced by Botrytis cinerea. Five oenological tannins were used (gallotannin, ellagitannin, quebracho, grape-skin and grape-seed) and compared with ascorbic acid (AA) and sulfur dioxide (SO2). Oenological tannins, AA and SO2 were added to botrytized must at different doses. After 4 minutes, laccase activity was measured by the syringaldazine method [2] using different concentration of subtract. Enzymatic kinetic constants (Km/Vmax) were determined according to Michaelis-Menten model. Furthermore, B. cinerea (strain 213) was grown in a stimulating liquid medium for laccase production [3]. The molecular weight (MW) and the effect of bentonite and tannins upon laccase were studied by SDS-PAGE. The results confirm that all oenological tannins inhibit laccase activity and that the higher the dose the lower the laccase activity. In this way, gallotannin, grape-seed and skins tannins seem to be the most effective tannins. All the tannins were as effective as AA, even though SO2 was clearly the most effective inhibitor. The laccase produced by B. cinerea had a MW of 95 kDa. After bentonite treatment all wine protein bands disappeared and laccase band decreased slightly although its activity remains stable. The interaction between different oenological tannins and laccase was analyzed by measuring the reduction of the intensity of the laccase band. In general, the reduction of band intensity correlates with the reduction of laccase enzymatic activity. 

It can be concluded therefore that oenological tannins a good candidate to prevent laccase effects, helping to diminish the SO2 dose in grapes infected by B. cinerea. 

[1] Vignault, A.; Pascual, O.; Jourdes, M.; Moine, V.; Fermaud, M.; Roudet, J.; Canals, J.M.; Teissedre, P-L.; Zamora, F. Oeno One, 2019. 
[2] Urbano Cuadrado, M.; Pérez-Juan, P.M.; Luque de Castro, M.; Gomez-Nieto, M.A., Anal Chim Acta, 2005, 553, 99-104. 
[3] Quijada-Morin, N.; Garcia, F.; Lambert, K.; Walker, A.S.; Tiers, L.; Viaud, M.; Sauvage, F-X.; Hirtz, C.; Saucier, C.; Aust J Grape Wine Res, 2018, 24, 241-251.

DOI:

Publication date: June 19, 2020

Issue: OENO IVAS 2019

Type: Article

Authors

Fernando Zamora (1), Adeline Vignault (2), Jordi Gombau (1), Michael Jourdes (2), Virginie Moine (3), Joan Miquel Canals (1), Pierre-Louis Teissedre (2)

1) Departament de Bioquímica i Biotecnologia. Facultat d’Enologia. Universitat Rovira i Virgili. C/ Marcel.lí Domingo 1, 43007-Tarragona (Spain)
2) Unitéde recherche Oenologie, EA 4577, USC 1366 INRA, ISVV, Universitéde Bordeaux, Bordeaux INP, F33882 Villenave d’Ornon France
3) Laffort, 11 rue Aristide berges, 33270 Floirac, France

Contact the author

Keywords

Oenological tannins, Botrytis cinerea laccase, Kinetics, SDS-PAGE 

Tags

IVES Conference Series | OENO IVAS 2019

Citation

Related articles…

Different soil types and relief influence the quality of Merlot grapes in a relatively small area in the Vipava Valley (Slovenia) in relation to the vine water status

Besides location and microclimatic conditions, soil plays an important role in the quality of grapes and wine. Soil properties influence…

Upscaling the integrated terroir zoning through digital soil mapping: a case study in the Designation of Origin Campo de Borja

homogeneous zones by intersecting several partial zonings of major factors that influence vineyard growth. Each of them follows specific process from their corresponding disciplines. Soil zoning specifically refers to a Soil Resource Inventory map that has traditionally been generated by conventional soil mapping methods. These methods have shortcomings in reaching fine cartographic and categorical details and involve significant expenses, which undermines their applicability. A new framework named Digital Soil Mapping has introduced quantitative models by statistical techniques to establish soil-landscape relationships and is able to provide intensive scale cartography.

In the present study, a microzoning at 1:10.000 scale is generated from an initial zoning, where the conventional soil map with polytaxic map units is replaced by a new one from digital techniques that disaggregates them. The comparison between the zonings considers a quantitative evaluation of capability for each Homogeneous Terroir Unit by means of the Viticultural Quality Index and its categorization based on its distribution by map. The spatial intersection of both maps gives rise to a confusion matrix in which the flows of class variations after the substitution are assessed.

The results show a five-fold increase in the number of Homogeneous Terroir Units identified and a larger differentiation among them, evidenced by a wider range in the capability index distribution. Both elements are accompanied by an increase in the detection of areas of higher potential within previously undervalued uniform zones.These features are a direct effect of the improvements brought by Digital Soil Mapping techniques and would verify the advantages of their implementation in the Integrated Terroir zoning. Eventually, such new highly detailed terroir units would benefit precision viticulture and sustainable management practices.

Spatiotemporal patterns of chemical attributes in Vitis vinifera L. cv. Cabernet Sauvignon vineyards in Central California

Spatial variability of vine productivity in winegrapes is important to characterise as both yield and quality are relevant for the production of different wine styles and products. The objectives were to understand how patterns of variability of Cabernet Sauvignon fruit composition changed over time and space, how these patterns could be characterised with indirect measurements, and how spatial patterns of the variation in fruit compositional attributes can aid in improving management. Prior to the 2017 vintage, 125 data vines were distributed across each of four vineyards in the Lodi American Viticultural Area (AVA) of California. Each data vine was sampled at commercial harvest in 2017, 2018, and 2019. Yield components and fruit composition were measured at harvest for each data vine, and maps of yield and fruit composition were produced for eight ‘objective measures of fruit quality’: total anthocyanins, polymeric tannins, quercetin glycosides, malic acid, yeast assimilable nitrogen, β-damascenone, C6 alcohols and aldehydes, and 3-isobutyl-2-methoxypyrazine. Patterns of variation in anthocyanins and phenolic compounds were found to be most stable over time. Given this relative stability, management decisions focused on fruit quality could be based on zonal descriptions of anthocyanins or phenolics to increase profitability in some vineyards. In each vineyard, dormant season pruning weights and soil cores were collected at each location, elevation and soil apparent electrical conductivity surveys were completed, and remotely sensed imagery was captured by fixed wing aircraft and two satellite platforms at major phenological stages. The data collected were used to develop relationships among biophysical data, soil, imagery, and fruit composition. The standardised and aggregated samples from four vineyards over three seasons were included in the estimation of ‘common variograms’ to assess how this technique could aid growers in producing geostatistically rigorous maps of fruit composition variability without cumbersome, single season sampling efforts.

Modeling island and coastal vineyards potential in the context of climate change

Climate change impacts regional and local climates, which in turn affects the world’s wine regions. In the short term, these modifications rises issues about maintaining quality and style of wine, and in a longer term about the suitability of grape varieties and the sustainability of traditional wine regions. Thus, adaptation to climate change represents a major challenge for viticulture. In this context, island and coastal vineyards could become coveted areas due to their specific climatic conditions. In regions subject to warming, the proximity of the sea can moderate extremes temperatures, which could be an advantage for wine. However, coastal and island areas are particular prized spaces and subject to multiple pressures that make the establishment or extension of viticulture complex.
In this perspective, it seems relevant to assess the potentialities of coastal and island areas for viticulture. This contribution will present a spatial optimization model that tends to characterize most suitable agroclimatic patterns in historical or emerging vineyards according to different scenarios. Thanks to an in-depth bibliography a global inventory of coastal and insular vineyards on a worldwide scale has been realized. Relevant criteria have been identified to describe the specificities of these vineyards. They are used as input data in the optimization process, which will optimize some objectives and spatial aspects. According to a predefined scenario, the objectives are set in three main categories associated with climatic characteristics, vineyards characteristics and management strategies. At the end of this optimization process, a series of maps presents the different spatial configurations that maximize the scenario objectives.

Influence of weather and climatic conditions on the viticultural production in Croatia

The research includes an analysis of the impact of weather conditions on phenological development of the vine and grape quality, through monitoring of four experimental cultivars (Chardonnay, Graševina, Merlot and Plavac mali) over two production years. In each experimental vineyard, which were evenly distributed throughout the regions of Slavonia and The Croatian Danube, Croatian Uplands,