terclim by ICS banner
IVES 9 IVES Conference Series 9 UNRAVELLING THE ROLE OF LACTIC ACID BACTERIA ON SPARKLING WINE ELABORATION THROUGH METABOLOMICS APPROACH

UNRAVELLING THE ROLE OF LACTIC ACID BACTERIA ON SPARKLING WINE ELABORATION THROUGH METABOLOMICS APPROACH

Abstract

Xinomavro is a red grape variety from Northern Greece (Protected Designation of Origin), known for the nice acidities, perfectly appropriate for sparkling wine production (Rosé and Blanc de Noir). The elaboration of sparkling wine requires technical as well as scientific skills. Although the impact of the yeast strains and their metabolites on the final product quality is well documented, the action of bacteria still remains unknown.

The present work focuses (i) on the population diversity of lactic acid bacteria isolated from sparkling wines and (ii) on the technological effect of the species during sparkling wine elaboration. Bacterial strains were typed by multiple loci VNTR analysis (MLVA) based on five tandem repeats loci and 3 different strains were chosen as starters for the sparkling wine production.

Xinomavro base wine was treated according to the winery production protocol and second fermentation was realised in the bottle under 6 different inoculation schemes.1) addition of S. cerevisiae (Lalvin DV10) 2) addition of S. cerevisiae (Lalvin DV10) and Lysozyme (40g/hL) 3) addition of S. cerevisiae (Lalvin DV10) and O. oeni Greek strain (UNIWA collection) 4) addition of S. cerevisiae (Lalvin DV10) and O. oeni French strain (CRBO collection) 5) addition of S. cerevisiae (Lalvin DV10) and O. oeni Commercial strain 6) addition of Schizo saccharomyces pombe strain (NRRL collection). Twelve months after the second fermentation in the bottle, oenological parameters were determined according to the OIV protocols, the volatile compounds produced were measured by GC/MS, and the metabolomic fingerprint analysis were acquired by an UPLC-HDMS-QTof-MS instrument. Finally, all produced wines were evaluated by quantitative descriptive sensorial analysis.

Malolactic fermentations were realized in all cases except the condition n°2 where lysozyme was added. Forty compounds were quantified and separated according to their chemical classes (monoterpenes, norisoprenoids, aldehydes, alcohols, esters, acids, and ketones) while statistical analysis showed the presence of three groups of sparkling wines according to the inoculation scheme. The untargeted metabolomic approach clearly discriminated the action of bacteria and revealed intra species variability at strain level. This is the first time that highlights the role of lactic acid bacteria and precisely of the species of O. oeni to sparkling wine elaboration.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Maria DIMOPOULOU1,2, Margot PAULIN1, Olivier CLAISSE1, Cécile MIOT-SERTIER1, Fotini DROSOU2, Panagiotis ARAPITSAS2,3, Marguerite DOLS-LAFARGUE1

1. Bordeaux, Bordeaux INP, INRAE, UMR OENO, UMR 1366, ISVV, F-33140 Villenave d’Ornon, France
2. Department of Wine, Vine, and Beverage Sciences, School of Food Science, University of West Attica, Athens, Greece
3. Department of Food Quality and Nutrition, Edmund Mach Foundation, Research and Innovation Centre, Via Edmund Mach 1, 38010 San Michele all’Adige, TN, Italy

Contact the author*

Keywords

sparkling wine, malolactic fermentation, Xinomavro, bacteria

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

EVALUATION OF INDIGENOUS CANADIAN YEAST STRAINS AS WINE STARTER CULTURES ON PILOT SCALE FERMENTATIONS

The interactions between geographical and biotic factors, along with the winemaking process, influence the composition and sensorial characteristics of wine¹. In addition to the primary end products of alcoholic fermentation, many secondary metabolites contribute to wine flavor and aroma and their production depends predominantly on the yeast strain carrying out the fermentation. Commercially available strains of S. cerevisiae help improve the reproducibility and predictability of wine quality. However, most commercial wine strains available on the market have been isolated from Europe, are genetically similar, and may not be the ideal strain to reflect the terroir of Canadian vineyards².

INFLUENCE OF THE NITROGEN / LIPIDS RATIO OF MUSTS ON THE REVELATION OF AROMATIC COMPOUNDS IN SAUVIGNON BLANC WINE

Production of volatile compounds by yeast is known to be modulated by must nitrogen. Nevertheless, various parameter of must quality have an impact on yeast fermentation. In this study we propose to evaluate the impact of nitrogen / lipids balance on a Sauvignon Blanc grape juice (Val de Loire).
Must was prepared from the same grapes at pilot scale. Three modalities were carried out: direct pressing, direct pressing with a pre-fermentation cold stabulation and pellicular maceration before pressing.

EVALUATION OF A SEAWEED EXTRACT OF RUGULOPTERYX OKAMURAE AGAINST ERYSIPHE NECATOR IN GRAPEVINE

Powdery mildew, caused by Erysiphe necator, is a widespread disease that causes high economical losses in viticulture. The main strategy to control the disease is the recurrent application of sulphur based phytochemical compounds. However, in order to reduce their accumulation in the environment and promote the sustainability of the sector, the European Commission has applied restrictions to the number of pesticide treatments and the maximum quantity of fungicides to be applied in viticulture. Seaweeds, in particular macroalgae, are marine resources rich in sulphated polysaccharides with bio-protective potential for the plant, representing an environmentally-friendly alternative approach for sustainable wine production.

AROMATIC AND FERMENTATIVE PERFORMANCES OF HANSENIASPORA VINEAE IN DIFFERENT SEQUENTIAL INOCULATION PROTOCOLS WITH SACCHAROMYCES CEREVISIAE FOR WHITE WINEMAKING

Hanseniaspora vineae (Hv) is a fermenting non-Saccharomyces yeast that compared to Saccharomyces cerevisiae (Sc) present some peculiar features on its metabolism that make it attractive for its use in wine production. Among them, it has been reported a faster yeast lysis and release of polysaccharides, as well as increased ß-glucosidase activity. Hv also produces distinctive aroma compounds, including elevated levels of fermentative compounds such as ß-phenylethyl acetate and norisoprenoids like safranal. However, it is known for its high nutritional requirements, resulting in prolonged and sluggish fermentations, even when complemented with Sc strain and nutrients.

Influence of agrophotovoltaic on vine and must in a cool climate

The current energy crisis means that interest in agrophotovoltaics has increased significantly. The reason behind this is that the system aims to combine agricultural production with energy production. During the three-year period from 2020 to 2022, the effects of photovoltaic panels on the vine, the yield and the quality of the must were studied in Walenstadt in northern Switzerland, an area with a cool, humid climate. 65 Pinot noir vines were planted in the 160m2 study area. Because of the large edge effects, only 3 repetitions with 4 vines each could be created. A significantly lower leaf infestation by Plasmopara viticola was observed among the panels in each of the three years.