terclim by ICS banner
IVES 9 IVES Conference Series 9 A NEW SPECIFIC LINEAGE OF OENOCOCCUS OENI IN COGNAC APPELLATION WINES

A NEW SPECIFIC LINEAGE OF OENOCOCCUS OENI IN COGNAC APPELLATION WINES

Abstract

Oenococcus oeni is the main lactic acid bacteria (LAB) species which conducts the malolactic fermentation (MLF) in wine. During MLF, O. oeni converts malic acid into lactic acid, which modulates wine aroma composition leading to better balanced organoleptic properties. O. oeni is a highly specialized species only detected in environments containing alcohol such as wine, cider or kombucha. Genome analysis of more than 240 strains showed that they form at least 4 main phylogenetic lineages and several sublineages, which are associated with different beverages or types of wines. Distilled wines produced in Cognac appellation of origin undergo MLF. Given the lack of knowledge of LAB present in distillation wines, the control of MLF and the further storage of wines is a difficult task. Therefore, the aim of this work is to analyze the biodiversity of O. oeni strains naturally occurring in cognac distilled wines and to determine if they confer a particular quality to the spirit after distillation.
559 samples of wines were collected before, during and after MLF from 24 wineries located in almost all the regions of Cognac appellation during 4 vintages from 2019 to 2022. The samples were processed to isolate single colonies of LAB, which were typed at the species and strain levels by MLVA (Multiple Loci of Variable Number of Tandem Repeats Analysis). About 5000 colonies of O. oeni isolates were obtained and assigned to 688 different strains. The most abundant strains in each winery were further analyzed at the genomic level. A total of 49 draft genomes were produced by Illumina MiSeq. The distances between these 49 genomes and 240 other publicly available O. oeni genomes were calculated using ANI (Average Nucleotide Identity) and used to reconstruct a phylogenetic tree. The tree showed that 34 of the 49 strains grouped together in a new phylogenetic lineage and contain only stains isolated from cognac wines. The 34 strains of this lineage represented more than half of the colonies isolated during MLF in the wineries, which suggests that this lineage is specific and predominant in all the cognac wines. Moreover, the same strains were often found in the same wineries during consecutive vintages. The results suggest that the strains of this genetic lineage share specific genetic properties conferring them a better adaptation to cognac wines, and may in addition confer specific aromatic characteristics to cognac wines during MLF.

 

1. Lorentzen, M.P.G., and Lucas, P.M. (2019). Distribution of Oenococcus oeni populations in natural habitats. Applied Microbio-logy and Biotechnology 103, 2937–2945.
2. Claisse, O., and Lonvaud-Funel, A. (2012). Development of a multilocus variable number of tandem repeat typing method for Oenococcus oeni. Food Microbiology 30, 340–347.
3. Claisse, O., and Lonvaud-Funel, A. (2014). Multiplex variable number of tandem repeats for Oenococcus oeni and applica-tions. Food Microbiology 38, 80–86

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Sayoko Matsumoto¹, Olivier Claisse¹, Cécile Miot-Sertier¹, Rebekah Hicks David², Valentin Lebrec², Amandine Bernier², Panagiotis Stamatopoulos², Xavier Poitou², Jana Rudolf¹, Patrick Lucas¹

1. Univ. Bordeaux, Bordeaux INP, INRAE, Bordeaux Sciences Agro, OENO, UMR 1366, ISVV, F-33140 Villenave d’Ornon, France
2. Hennessy, Rue de la Richonne, CS20020 – 16100, Cognac Cedex, France

Contact the author*

Keywords

Oenococcus oeni, Malolactic fermentation, Cognac, Biodiversity

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

EXPLORING THE METABOLIC AND PHENOTYPIC DIVERSITY OF INDIGENOUS YEASTS ISOLATED FROM GREEK WINE

Climate change leads to even more hostile and stressful for the wine microorganism conditions and consequently issues with fermentation rate progression and off-character formation are frequently observed. The objective of the current research was to classify a great collection of yeast isolates from Greek wines based on their technological properties with oenological interest. Towards this direction, fourteen spontaneously fermented wines from different regions of Greece were collected for further yeast typing. The yeast isolates were subjected in molecular analyses and identification at species level.

MAPPING THE CONCENTRATIONS OF GASEOUS ETHANOL IN THE HEADSPACE OF CHAMPAGNE GLASSES THROUGH INFRARED LASER ABSORPTION SPECTROSCOPY

Under standard wine tasting conditions, volatile organic compounds (VOCs) responsible for the wine’s bouquet progressively invade the glass headspace above the wine surface. Most of wines being complex water/ethanol mixtures (with typically 10-15 % ethanol by volume), gaseous ethanol is therefore undoubtedly the most abundant VOC in the glass headspace [1]. Yet, gaseous ethanol is known to have a multimodal influence on wine’s perception [2]. Of particular importance to flavor perception is the effect of ethanol on the release of aroma compounds into the headspace of the beverage [1].

EVALUATION OF A SEAWEED EXTRACT OF RUGULOPTERYX OKAMURAE AGAINST ERYSIPHE NECATOR IN GRAPEVINE

Powdery mildew, caused by Erysiphe necator, is a widespread disease that causes high economical losses in viticulture. The main strategy to control the disease is the recurrent application of sulphur based phytochemical compounds. However, in order to reduce their accumulation in the environment and promote the sustainability of the sector, the European Commission has applied restrictions to the number of pesticide treatments and the maximum quantity of fungicides to be applied in viticulture. Seaweeds, in particular macroalgae, are marine resources rich in sulphated polysaccharides with bio-protective potential for the plant, representing an environmentally-friendly alternative approach for sustainable wine production.

WINE RACKING IN THE WINERY AND THE USE OF INERT GASES: CONTROL AND OPTIMIZATION OF THE PROCESS

Atmospheric oxygen (O₂) generates oxidation in wines that affect their physicochemical and sensory evolution. The O₂ uptake in the different winemaking processes is generally considered to be negative for the sensory characteristics of white and rosé wines. Wine racking is a critical point of O₂ uptake, as the large surface area of the wine exposed during this operation and the inability to maintain an effective inert gas blanket over it.
The aim was to study the uptake of O₂ during the racking of a model wine as a reference and to compare with purging the destination tank with different inert gases.

MODELLING THE AGEING POTENTIAL OF SYRAH RED WINES BY ACCELERATED AGEING TESTS: INFLUENCE OF ANTIOXIDANT ASSAYS AND PHENOLIC COMPOSITION

Red wine ageing is an important step in the red wine evolution and impacts its chemical and sensory characteristics through many chemicals and physico-chemical reactions. The kinetics of these evolutions depend on the wine studied and influence the wine ageing potential. Generally, high quality red wines require a longer period of bottle ageing before consumption¹. The ageing potential is an impor-tant parameter for wine quality and is related to the capacity of a wine to undergo oxidation over time². Phenolic compounds which are ones of the main substrates for oxidation can then potentially modulate ageing potential³.