terclim by ICS banner
IVES 9 IVES Conference Series 9 A NEW SPECIFIC LINEAGE OF OENOCOCCUS OENI IN COGNAC APPELLATION WINES

A NEW SPECIFIC LINEAGE OF OENOCOCCUS OENI IN COGNAC APPELLATION WINES

Abstract

Oenococcus oeni is the main lactic acid bacteria (LAB) species which conducts the malolactic fermentation (MLF) in wine. During MLF, O. oeni converts malic acid into lactic acid, which modulates wine aroma composition leading to better balanced organoleptic properties. O. oeni is a highly specialized species only detected in environments containing alcohol such as wine, cider or kombucha. Genome analysis of more than 240 strains showed that they form at least 4 main phylogenetic lineages and several sublineages, which are associated with different beverages or types of wines. Distilled wines produced in Cognac appellation of origin undergo MLF. Given the lack of knowledge of LAB present in distillation wines, the control of MLF and the further storage of wines is a difficult task. Therefore, the aim of this work is to analyze the biodiversity of O. oeni strains naturally occurring in cognac distilled wines and to determine if they confer a particular quality to the spirit after distillation.
559 samples of wines were collected before, during and after MLF from 24 wineries located in almost all the regions of Cognac appellation during 4 vintages from 2019 to 2022. The samples were processed to isolate single colonies of LAB, which were typed at the species and strain levels by MLVA (Multiple Loci of Variable Number of Tandem Repeats Analysis). About 5000 colonies of O. oeni isolates were obtained and assigned to 688 different strains. The most abundant strains in each winery were further analyzed at the genomic level. A total of 49 draft genomes were produced by Illumina MiSeq. The distances between these 49 genomes and 240 other publicly available O. oeni genomes were calculated using ANI (Average Nucleotide Identity) and used to reconstruct a phylogenetic tree. The tree showed that 34 of the 49 strains grouped together in a new phylogenetic lineage and contain only stains isolated from cognac wines. The 34 strains of this lineage represented more than half of the colonies isolated during MLF in the wineries, which suggests that this lineage is specific and predominant in all the cognac wines. Moreover, the same strains were often found in the same wineries during consecutive vintages. The results suggest that the strains of this genetic lineage share specific genetic properties conferring them a better adaptation to cognac wines, and may in addition confer specific aromatic characteristics to cognac wines during MLF.

 

1. Lorentzen, M.P.G., and Lucas, P.M. (2019). Distribution of Oenococcus oeni populations in natural habitats. Applied Microbio-logy and Biotechnology 103, 2937–2945.
2. Claisse, O., and Lonvaud-Funel, A. (2012). Development of a multilocus variable number of tandem repeat typing method for Oenococcus oeni. Food Microbiology 30, 340–347.
3. Claisse, O., and Lonvaud-Funel, A. (2014). Multiplex variable number of tandem repeats for Oenococcus oeni and applica-tions. Food Microbiology 38, 80–86

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Sayoko Matsumoto¹, Olivier Claisse¹, Cécile Miot-Sertier¹, Rebekah Hicks David², Valentin Lebrec², Amandine Bernier², Panagiotis Stamatopoulos², Xavier Poitou², Jana Rudolf¹, Patrick Lucas¹

1. Univ. Bordeaux, Bordeaux INP, INRAE, Bordeaux Sciences Agro, OENO, UMR 1366, ISVV, F-33140 Villenave d’Ornon, France
2. Hennessy, Rue de la Richonne, CS20020 – 16100, Cognac Cedex, France

Contact the author*

Keywords

Oenococcus oeni, Malolactic fermentation, Cognac, Biodiversity

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

MAPPING THE CONCENTRATIONS OF GASEOUS ETHANOL IN THE HEADSPACE OF CHAMPAGNE GLASSES THROUGH INFRARED LASER ABSORPTION SPECTROSCOPY

Under standard wine tasting conditions, volatile organic compounds (VOCs) responsible for the wine’s bouquet progressively invade the glass headspace above the wine surface. Most of wines being complex water/ethanol mixtures (with typically 10-15 % ethanol by volume), gaseous ethanol is therefore undoubtedly the most abundant VOC in the glass headspace [1]. Yet, gaseous ethanol is known to have a multimodal influence on wine’s perception [2]. Of particular importance to flavor perception is the effect of ethanol on the release of aroma compounds into the headspace of the beverage [1].

IN DEPTH CHARACTERIZATION OF OENOLOGICAL CHARACTERISTICS OF TWO LACHANCEA THERMOTOLERANS STARTER STRAINS

Non-Saccharomyces starter cultures became increasingly popular over the years because of their potential to produce more distinctive and unique wines. The major benefit of the use of Lachancea thermotolerans as a fermentation starter is its ability to produce relevant amounts of lactic acid and reduce alcoholic strength, making it valuable for mitigating negative impacts of climate change on grapes and wine quality. Besides, like any other non-Saccharomyces yeast, L. thermotolerans can significantly affect a whole range of other physico-chemical wine parameters.

EXPLORING RED WINE TYPICITY OF CORBIÈRES: EVALUATION OF THE DEGREE OF IMPACT OF VINIFICATION PROCESS ON THE CHEMICAL COMPOSITION AND ORGANOLEPTIC PROPERTIES OF WINES FROM DIFFERENT TERROIR

It is important nowadays for wine producers to create a product that is an expression of their terroir, a concept including the interaction between a place (topography, climate, soil), the people (tradition, winemaking and viticultural practices) and the resulting product (grape varieties, wines) [1]. Nonetheless, wine’s typicity linked to those terroirs must be easily recognizable by consumers thanks to distinctive sensory characters and composition [2]. Among the compounds of interest, aromatic compounds and polyphenols play an important role in the quality of red wines, by impacting on the odour, color and astringency. To explore the influence of terroir factors, including climate, soil and human practices, on the chemical and sensory profile of wines, red wines from five terroirs of the Corbières appellation were subjected to a general study approach.

TARTARIC STABILIZATION MAY AFFECT THE COLOR AND POLYPHENOLIC COMPOSITION OF TANNAT RED WINES FROM URUGUAY

Tartrate precipitation affects the properties of wines, due to the formation of crystals that cause turbidity, even after being bottled. The forced tartaric stabilization is carried out frequently for young wines, through various physicochemical procedures. The traditional treatment for tartaric stabilization is refrigeration, but it can have a negative effect on wine’s sensory properties, and particularly on the color of red wines. The aim of this study was to evaluate the effect of different tartaric stabilization options on the color and phenolic composition of Tannat red wines from Uruguay.

TOWARDS THE SHELF-LIFE PREDICTION OF OLD CHAMPAGNE VINTAGES DEPENDING ON THE BOTTLE CAPACITY

Today, nearly one billion bottles of different sizes and capacities are aging in Champagne cellars while waiting to be put on the market. Among them, several tens of thousands of prestigious cuvees elaborated prior the 2000s are potentially concerned by prolonged aging on lees. However, when it comes to champagne tasting, dissolved CO₂ is a key compound responsible for the very much sought-after effer-vescence in glasses [1]. Yet, the slow decrease of dissolved CO₂ during prolonged aging of the most prestigious cuvees raises the issue of how long a champagne can age before it becomes unable to form CO₂ bubbles during tasting [2].