terclim by ICS banner
IVES 9 IVES Conference Series 9 EVALUATION OF INDIGENOUS CANADIAN YEAST STRAINS AS WINE STARTER CULTURES ON PILOT SCALE FERMENTATIONS

EVALUATION OF INDIGENOUS CANADIAN YEAST STRAINS AS WINE STARTER CULTURES ON PILOT SCALE FERMENTATIONS

Abstract

The interactions between geographical and biotic factors, along with the winemaking process, influence the composition and sensorial characteristics of wine¹. In addition to the primary end products of alcoholic fermentation, many secondary metabolites contribute to wine flavor and aroma and their production depends predominantly on the yeast strain carrying out the fermentation. Commercially available strains of S. cerevisiae help improve the reproducibility and predictability of wine quality. However, most commercial wine strains available on the market have been isolated from Europe, are genetically similar, and may not be the ideal strain to reflect the terroir of Canadian vineyards². Instead, indigenous S. cerevisiae strains may enhance the typical sensory properties and characteristic profile of the wine region³. The Okanagan Valley is the major wine-producing region in British Columbia, Canada. The Measday lab has isolated S. cerevisiae indigenous strains from Okanagan Valley vineyards that are genetically distinct from commercial strains⁴. After evaluating the oenological characteristics of six indigenous strains isolated from Okanagan Crush Pad (OCP) winery in laboratory-scale fermentations, two were selected for pilot-scale winery fermentations to assess their potential as wine starter cultures. Fermentations with OCP088 and OCP125 yeast strains were carried out in triplicate 250L stainless steel barrels at OCP winery. Vin Gris (VG, Pinot Noir) and Pinot Gris (PG) varietals were chosen, the grapes were pressed, and the juice was settled to remove skins before inoculation. Major metabolites (organic acids, sugars, and ethanol) were quantified using HPLC-RID, sugar in both wines was mainly fructose, ranging between 16 g/L and 20 g/L, ABV of the finished product ranged between 10.8 and 11.3 %. Volatile compounds (terpenes, esters, ketones, and higher alcohols) were identified using SPME-GC/MS We identified the following number of volatile compounds in each fermentation: OCP125 PG (56), OCP088 PG (52), OCP125 VG (45), OCP088 VG (44). The majority of volatile compounds were esters, which are known for their contribution to wine quality. OCP 125 tended to produce more terpenes than OCP 088. Some of these compounds are responsible for honey and grapefruit-like aromas, which are atypical of these varietals, adding to the complexity of the final product.

 

1. Welke, J. E., Zanus, M., Lazarotto, M., Schmitt, K. G., & Zini, C. A.. (2012) Volatile Characterization by Multivariate Optimization of Headspace-Solid Phase Microextraction and Sensorial Evaluation of Chardonnay Base Wines. Journal of the Brazilian Chemical Society, 23(J. Braz. Chem. Soc., 2012 23(4)). doi: 10.1590/S0103-50532012000400013
2. Borneman, Anthony & Forgan, Angus & Kolouchova, Radka & Fraser, James & Schmidt, Simon. (2016). Whole Genome Comparison Reveals High Levels of Inbreeding and Strain Redundancy Across the Spectrum of Commercial Wine Strains of Saccharomyces cerevisiae. G3 (Bethesda, Md.). 6. doi: 10.1534/g3.115.025692.
3. Nikolaou, E., Soufleros, E., Bouloumpasi, E., Tzanetakis N. (2006) Selection of indigenous Saccharomyces cerevisiae strains according to their oenological characteristics and vinification results. Food Microbiology 23, 205-211 doi:10.1016/j. fm.2005.03.004
4. Cheng, E., Martiniuk, J.T., Hamilton, J., McCarthy, M., Castellarin, S., and Measday, V. (2020). Characterization of Sub-Regional Variation in Saccharomyces Populations and Phenolic Composition in a Canadian Wine Region. Frontiers in Genetics 11, 1-19. doi: 10.3389/fgene.2020.00908.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Vivien Measday¹.

1. Wine Research Center, Faculty of Land and Food Systems, University of British Columbia, Canada

Contact the author*

Keywords

Indigenous strains, metabolites, volatile compounds, wine fermentation

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

INVESTIGATING TERROIR TYPICITY: A COMPREHENSIVE STUDY BASED ON THE AROMATIC AND SENSORIAL PROFILES OF RED WINES FROM CORBIÈRES APPELLATION

Volatile compounds play a significant role on the organoleptic properties defining wines quality. This particular role was exploited in several studies with the aim to differentiate wines from a more or less extensive production area, according to their sensory profile [1], as well as their chemical composition [2,3] (Di Paola-Naranjo et al., 2011; Kustos et al., 2020). Indeed, since aroma compounds development in grapes depends primarily on the environmental conditions of the vines and grapes (soil and climate), it is conceivable that these parameters craft the aromatic signature of the wine produced, in relation to its origin (Van Leeuwen et al., 2020). In this work, a general study on the aromatic and sensorial profile of wines produced in five sub-regions of the Corbières denomination, a renowned red grape varieties viticultural region in South France, was reported.

BIOSORPTION OF UNDESIRABLE COMPONENTS FROM WINE BY YEAST-DERIVED PRODUCTS

4-Ethylphenol (EP) in wine is associated with organoleptic defects such as barn and horse sweat odors. The origin of EP is the bioconversion reaction of p-coumaric acid (CA), naturally present in grapes and grape musts by contaminating yeasts of the genus Brettanomyces bruxellensis.
Yeast cell walls (YCW) have shown adsorption capacities for different compounds. They could be applied to wines in order to adsorb either CA and/or EP and thus reduce the organoleptic defects caused by the contaminating yeasts.

PINKING PHENOMENA ON WHITE WINES: RELATION BETWEEN PINKING SUSCEPTIBILITY INDEX (PSI) AND WINE ANTHOCYANINS CONTENT

Pinking is the emergence of pink tones in white wines exclusively produced from white grape varieties, known as pinking phenomena for many years. Pinking is essentially appeared when white wines are produced under reducing conditions [1,2,3]. Pinking usually occurs after bottling and storage of white wines, but its appearance has also been described after alcoholic fermentation or even as soon as the grape must is extracted [4]. Therefore, the purpose of this work was to investigate the existence of an-thocyanins in white wines made from different white grape varieties and grown locations and critically evaluate the most common method used for predicting pinking appearance in white wines: the Pinking Susceptibility Index (PSI).

FREE TERPENE RESPONSE OF ‘MOSCATO BIANCO’ VARIETY TO GRAPE COLD STORAGE

Temperature control is crucial in wine production, starting from grape harvest to the bottled wine storage. Climate change and global warming affect the timing of grape ripening, and harvesting is often done during hot summer days, influencing berry integrity, secondary metabolites potential, enzyme and oxidation phenomena, and even fermentation kinetics. To curb this phenomenon, pre-fermentative cold storage can help preserve the grapes and possibly increase the concentration of key secondary metabolites. In this study, the effect of grape pre-fermentative cold storage was assessed on the ‘Moscato bianco’ white grape cultivar, known for its varietal terpenes (65% of free terpenes represented by linalool and its derivatives) and widely used in Piedmont (Italy) to produce Asti DOCG wines.

MONOSACCHARIDE COMPOSITION AND POLYSACCHARIDE FAMILIES OF LYOPHILISED EXTRACTS OBTAINED FROM POMACES OF DIFFERENT WHITE GRAPE VARIETIES

The recovery of bioactive compounds from grape and wine by-products is currently an important and necessary objective for sustainability. Grape pomace is one of the main by-products and is a rich source of some bioactive compounds such as polyphenols, polysaccharides, fatty acids, minerals and seed oil. Polysaccharides contained in the grape cell wall can be rhamnogalacturonans type II (RG-II), polysaccharides rich in arabinose and galactose (PRAG), mannoproteins (MP), homogalacturonans (HG) and non pectic polysaccharides (NPP).