terclim by ICS banner
IVES 9 IVES Conference Series 9 EVALUATION OF INDIGENOUS CANADIAN YEAST STRAINS AS WINE STARTER CULTURES ON PILOT SCALE FERMENTATIONS

EVALUATION OF INDIGENOUS CANADIAN YEAST STRAINS AS WINE STARTER CULTURES ON PILOT SCALE FERMENTATIONS

Abstract

The interactions between geographical and biotic factors, along with the winemaking process, influence the composition and sensorial characteristics of wine¹. In addition to the primary end products of alcoholic fermentation, many secondary metabolites contribute to wine flavor and aroma and their production depends predominantly on the yeast strain carrying out the fermentation. Commercially available strains of S. cerevisiae help improve the reproducibility and predictability of wine quality. However, most commercial wine strains available on the market have been isolated from Europe, are genetically similar, and may not be the ideal strain to reflect the terroir of Canadian vineyards². Instead, indigenous S. cerevisiae strains may enhance the typical sensory properties and characteristic profile of the wine region³. The Okanagan Valley is the major wine-producing region in British Columbia, Canada. The Measday lab has isolated S. cerevisiae indigenous strains from Okanagan Valley vineyards that are genetically distinct from commercial strains⁴. After evaluating the oenological characteristics of six indigenous strains isolated from Okanagan Crush Pad (OCP) winery in laboratory-scale fermentations, two were selected for pilot-scale winery fermentations to assess their potential as wine starter cultures. Fermentations with OCP088 and OCP125 yeast strains were carried out in triplicate 250L stainless steel barrels at OCP winery. Vin Gris (VG, Pinot Noir) and Pinot Gris (PG) varietals were chosen, the grapes were pressed, and the juice was settled to remove skins before inoculation. Major metabolites (organic acids, sugars, and ethanol) were quantified using HPLC-RID, sugar in both wines was mainly fructose, ranging between 16 g/L and 20 g/L, ABV of the finished product ranged between 10.8 and 11.3 %. Volatile compounds (terpenes, esters, ketones, and higher alcohols) were identified using SPME-GC/MS We identified the following number of volatile compounds in each fermentation: OCP125 PG (56), OCP088 PG (52), OCP125 VG (45), OCP088 VG (44). The majority of volatile compounds were esters, which are known for their contribution to wine quality. OCP 125 tended to produce more terpenes than OCP 088. Some of these compounds are responsible for honey and grapefruit-like aromas, which are atypical of these varietals, adding to the complexity of the final product.

 

1. Welke, J. E., Zanus, M., Lazarotto, M., Schmitt, K. G., & Zini, C. A.. (2012) Volatile Characterization by Multivariate Optimization of Headspace-Solid Phase Microextraction and Sensorial Evaluation of Chardonnay Base Wines. Journal of the Brazilian Chemical Society, 23(J. Braz. Chem. Soc., 2012 23(4)). doi: 10.1590/S0103-50532012000400013
2. Borneman, Anthony & Forgan, Angus & Kolouchova, Radka & Fraser, James & Schmidt, Simon. (2016). Whole Genome Comparison Reveals High Levels of Inbreeding and Strain Redundancy Across the Spectrum of Commercial Wine Strains of Saccharomyces cerevisiae. G3 (Bethesda, Md.). 6. doi: 10.1534/g3.115.025692.
3. Nikolaou, E., Soufleros, E., Bouloumpasi, E., Tzanetakis N. (2006) Selection of indigenous Saccharomyces cerevisiae strains according to their oenological characteristics and vinification results. Food Microbiology 23, 205-211 doi:10.1016/j. fm.2005.03.004
4. Cheng, E., Martiniuk, J.T., Hamilton, J., McCarthy, M., Castellarin, S., and Measday, V. (2020). Characterization of Sub-Regional Variation in Saccharomyces Populations and Phenolic Composition in a Canadian Wine Region. Frontiers in Genetics 11, 1-19. doi: 10.3389/fgene.2020.00908.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Vivien Measday¹.

1. Wine Research Center, Faculty of Land and Food Systems, University of British Columbia, Canada

Contact the author*

Keywords

Indigenous strains, metabolites, volatile compounds, wine fermentation

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

2-YEARS STUDY ON COMPARISON BETWEEN THE VOLATILE CHEMICAL PROFILE OF TWO DIFFERENT BLENDS FOR THE ENHANCEMENT OF “VALPOLICELLA SUPERIORE”

Valpolicella is a famous wine producing region in the province of Verona owing its fame above all to the production of two Protected Designation of Origins (PDOs) withered wines: Amarone and Recioto. In recent years, however, the wineries have been interested in the enhancement and qualitative increase of another PDO, Valpolicella Superiore. All the Valpolicella PDOs wines are produced with a unique grape blend, mainly Corvina, Corvinone, Rondinella and a range of other minor varieties.From 2019 Valpolicella product regulation has changed the grape proportion of the blend allowing new composition parameters of wines. For this reason, studying the volatile chemical profiles to support wine makers in the effort to produce high quality wines represents a field of great interest.

EFFECTS OF LEAF REMOVAL AT DIFFERENT BUNCHES PHENOLOGICAL STAGES ON FREE AND GLYCOCONJUGATE AROMAS OF SKINS AND PULPS OF TWO ITALIAN RED GRAPES

Canopy-management practices are applied in viticulture to improve berries composition and quality, having a great impact on primary and secondary grape metabolism. Among these techniques, cluster zone leaf removal (defoliation) is widely used to manage air circulation, temperature and light radiation of grape bunches and close environment. Since volatiles are quantitatively and qualitatively influenced by the degree of fruit ripeness, the level of solar exposure, and the thermal environment in which grapes ripen, leaf removal has been shown to affect volatile composition of grape berries [1].

METHYL SALICYLATE: A TRENDY COMPOUND MARKER OF ZELEN, A UNIQUE SLOVENIAN VARIETY

The wine market interest for autochthonous varieties, particularly from less known wine regions, has significantly raised in the past few years. In that context, Slovenia, a small country from central Europe with a long winemaking tradition, is getting more and more attention, particularly through its range of unique regional varieties. Among them, Zelen, meaning “green” in Slovene, can only be found in the Vipava valley region, located on the western side of the country, near the border with Italy. When they are young, Zelen wines display very singular aromas reminiscent of rosemary, sage and white fruit. Despite its uniqueness, Zelen wine aromatic typicality is poorly documented in the literature.

SENSORY DEFINITION OF A TECHNICAL UNAVOIDABLE TRANSFER OF AROMA COMPOUNDS VIA SEALING IN A BOTTLING LINE IN ORDER TO PREVENT PROSECUTION DUE TO FRAUDULENT AROMATIZATION OF A SUBSEQUENTLY FILLED WINE

In 2020, 12% of all bottled German wines were aromatized, which may increase further due to rising popularity of dealcoholized wines. As sealing polymers of a bottling line absorb aroma compounds and may release them into regular wines in the next filling¹, this unintentional carry-over bears the risk to violate the legal ban of any aromatization of regular wine. However, following EU legislation, German food control authorities accept a technical unavoidable transfer of aroma compounds, if this is of no sensory significance.

THE INFLUENCE OF COMMERCIAL SACCHAROMYCES CEREVISIAE ON THE POLY-SACCHARIDES AND OTHER CHEMICAL PROFILES OF NEW ZEALAND PINOT NOIR WINES

Wine polysaccharides (PS) play an important role in balancing mouthfeel and stability of wine and even influence aroma volatility. Despite this, there is limited research into the effect of winemaking additives on the polysaccharide profile and other macromolecules of New Zealand (NZ) Pinot noir wine. In this study the influence of a selection of commercial S. cerevisiae strains on the chemical profile, including polysaccharides, of New Zealand Pinot noir (PN) wine was investigated. Research scale PN fermentations using five strains of commercially available S. cerevisiae (Lalvin EC1118 and RC212, Levuline BRG YSEO, Viallate Ferm R71 and R82) were undertaken. PS were qualified and quantified using HPLC-RID.