terclim by ICS banner
IVES 9 IVES Conference Series 9 IMPACT OF RHIZOPUS AND BOTRYTIS ON WINE FOAMING PROPERTIES

IMPACT OF RHIZOPUS AND BOTRYTIS ON WINE FOAMING PROPERTIES

Abstract

A lot of work has been done on the impact of Botrytis on the foam of sparkling wines. This work often concerns wines produced in cool regions, where Botrytis is the dominant fungal pathogen. However, in southern countries such as Spain, in particularly hot years such as 2022, the majority fungal pathogen is sometimes Rhizopus. Like Botrytis, Rhizopus is a fungus that produces an aspartic protease. The aim of this study was therefore to determine the foaming properties of wines produced with a synthetic must contaminated by a Rhizopus or Botrytis culture. In order to confirm the identification of the fungal strain, the D1-D3 domains of the 28S rRNA gene were amplified and sequenced. BLAST search indicated 100% identity with a reference strain of Rhizopus lyococcus (CBS 320.35).

The complete experimental design presents 12 modalities (AF in triplicate, i.e. 36 bottles). The fungal isolates of Botrytis cinerea (B. c.) and Rhizopus lyococcus (R. l.) were cultured using a modified version of the method described by Gimenez et al. (2022). Alcoholic fermentations (AF) were performed in 500mL glass bottles from synthetic grape must supplemented or not with 50 mg/L of epicatechin. The yeast strain S. cerevisiae Lalvin EC1118 (Lallemand) was used for the AF process. To examine the impact of the pathoge- nic fungi, 10% (v/v) of B.c. or R.l. culture were added (separately) to the model grape juice. Furthermore, two different concentrations of L-malic acid were added to the fermentation media creating two sets of conditions : 2g/L of L-malic acid (pH=3.5) and 6 g/L of L-malic acid (pH=3). The results of the wines with fungus were compared to those of the control wines obtained without fungus.

The results of this study show that the presence of Rhizopus in the must significantly or highly significantly degrades the foamability and foam stability of the wines (foam measured with the KRUSS DFA100 equipment). The analysis of the protein composition by SDS-PAGE clearly shows a degradation of the yeast proteins by the fungal proteases of Rhizopus. Surprisingly, the Botrytis strain used did not affect the foam of the wines. These differences in proteolytic activity are confirmed by using BSA as a subs- trate: the Rhizopus culture degrades the 500 mg/L BSA in a few minutes, whereas the BSA degradation by the Botrytis culture remains considerably lower despite the longer culture of the fungus. Finally, the presence of epicatechin did not affect the wines’ foaming properties.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Richard Marchal¹, Candela Ruiz De Villa Sardón², Arnau Just Borràs², Nicolas Rozès², Fernando Zamora Marín², Joan-Miquel Canals Bosch², Thomas Salmon¹, José Francisco Cano Lira³, Jacques-Emmanuel Barbier4, Sabine Gognies¹

1. Université de Reims Champagne-Ardenne, Faculté des Sciences, Laboratoire d’Oenologie, 51687 Reims CEDEX 02, France
2. Universitat Rovira i Virgili, Facultat d’Enologia, Campus Sescelades, 43007 Tarragona, Spain
3. Un+iversitat Rovira i Virgili, Mycology, Environmental Microbiology Unit, Medicine Faculty / Oenology Faculty,  Sant Llorenç 21, 43201-Reus, Spain
4. Institut Œnologique de Champagne – ZI de Mardeuil – 51201 Épernay Cedex, France 

Contact the author*

Keywords

wine foam, Rhizopus, yeast proteins, aspartic protease

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

DISCRIMINATION OF BOTRYTIS CINEREA INFECTED GRAPES USING UNTARGE-TED METABOLOMIC ANALYSIS WITH DIRECT ELECTROSPRAY IONISATION MASS SPECTROMETRY

Infection of grapes (Vitis vinifera) by Botrytis cinerea (grey mould) is a frequent occurrence in vineyards and during prolonged wet and humid conditions can lead to significant detrimental impact on yield and overall quality. Growth of B. cinerea causes oxidisation of phenolic compounds resulting in a loss of colour and formation of a suite of off-flavours and odours in wine made from excessively infected fruit. Apart from wine grapes, developing post-harvest B. cinerea infection in high-value horticultural products during storage, shipment and marketing may cause significant loss in fresh fruits, vegetables and other crops. A rapid and sensitive assessment method to detect, screen and quantify fungal infection would greatly assist viticultural growers and winemakers in determining fruit quality.

NEW INSIGHTS INTO THE EFFECT OF TORULASPORA DELBRUECKII/SACCHAROMYCES CEREVISIAE INOCULATION STRATEGY ON MALOLACTIC FERMENTATION PERFORMANCE

Winemaking is influenced by micro-organisms, which are largely responsible for the quality of the product. In this context, Non-Saccharomyces and Saccharomyces species are of great importance not only because it influences the development of alcoholic fermentation (AF) but also on the achievement of malolactic fermentation (MLF). Among these yeasts, Torulaspora delbrueckii allows in sequential inoculation with strains of S. cerevisiae shorter MLF realizations [5] . Little information is available on the temporal effect of the presence of T. delbrueckii on (i) the evolution of AF and (ii) the MLF performance.

EFFECTS OF BIODYNAMIC VINEYARD MANAGEMENT ON GRAPE RIPENING MECHANISMS

Biodynamic agriculture, founded in 1924 by Rudolph Steiner, is a form of organic agriculture. Through a holistic approach, biodynamic agriculture seeks to preserve the diversity of agriculture and the existing interactions between the mineral world and the different components of the organic world. Biodynamic grape production involves the use of composts, herbal teas and mineral preparations such as 500, 501 and CBMT.
Several scientific studies have provided evidence on the effects of biodynamic farming on the soil, the plant and the wine. Numerous empirical opinions of wine growers support the existence of differences brought by such a management.

VOLATILE AND GLYCOSYLATED MARKERS OF SMOKE IMPACT: LEVELS AND PATTERNS OBSERVED IN 2020 WINES FROM THE UNITED STATES WEST COAST

Smoke impact in wines is caused by a wide range of volatile phenols found in wildfire smoke. These compounds are absorbed and accumulate in berries, where they may also become glycosylated. Both volatile and glycosylated forms eventually end up in wine where they can cause off-flavors, described as “smoky”, “bacon”, “campfire” and “ashtray”, often long-lasting and lingering on the palate. In cases of large wildfire events, economic losses for all wine industry actors can be devastating.

IN DEPTH CHARACTERIZATION OF OENOLOGICAL CHARACTERISTICS OF TWO LACHANCEA THERMOTOLERANS STARTER STRAINS

Non-Saccharomyces starter cultures became increasingly popular over the years because of their potential to produce more distinctive and unique wines. The major benefit of the use of Lachancea thermotolerans as a fermentation starter is its ability to produce relevant amounts of lactic acid and reduce alcoholic strength, making it valuable for mitigating negative impacts of climate change on grapes and wine quality. Besides, like any other non-Saccharomyces yeast, L. thermotolerans can significantly affect a whole range of other physico-chemical wine parameters.