terclim by ICS banner
IVES 9 IVES Conference Series 9 IMPACT OF RHIZOPUS AND BOTRYTIS ON WINE FOAMING PROPERTIES

IMPACT OF RHIZOPUS AND BOTRYTIS ON WINE FOAMING PROPERTIES

Abstract

A lot of work has been done on the impact of Botrytis on the foam of sparkling wines. This work often concerns wines produced in cool regions, where Botrytis is the dominant fungal pathogen. However, in southern countries such as Spain, in particularly hot years such as 2022, the majority fungal pathogen is sometimes Rhizopus. Like Botrytis, Rhizopus is a fungus that produces an aspartic protease. The aim of this study was therefore to determine the foaming properties of wines produced with a synthetic must contaminated by a Rhizopus or Botrytis culture. In order to confirm the identification of the fungal strain, the D1-D3 domains of the 28S rRNA gene were amplified and sequenced. BLAST search indicated 100% identity with a reference strain of Rhizopus lyococcus (CBS 320.35).

The complete experimental design presents 12 modalities (AF in triplicate, i.e. 36 bottles). The fungal isolates of Botrytis cinerea (B. c.) and Rhizopus lyococcus (R. l.) were cultured using a modified version of the method described by Gimenez et al. (2022). Alcoholic fermentations (AF) were performed in 500mL glass bottles from synthetic grape must supplemented or not with 50 mg/L of epicatechin. The yeast strain S. cerevisiae Lalvin EC1118 (Lallemand) was used for the AF process. To examine the impact of the pathoge- nic fungi, 10% (v/v) of B.c. or R.l. culture were added (separately) to the model grape juice. Furthermore, two different concentrations of L-malic acid were added to the fermentation media creating two sets of conditions : 2g/L of L-malic acid (pH=3.5) and 6 g/L of L-malic acid (pH=3). The results of the wines with fungus were compared to those of the control wines obtained without fungus.

The results of this study show that the presence of Rhizopus in the must significantly or highly significantly degrades the foamability and foam stability of the wines (foam measured with the KRUSS DFA100 equipment). The analysis of the protein composition by SDS-PAGE clearly shows a degradation of the yeast proteins by the fungal proteases of Rhizopus. Surprisingly, the Botrytis strain used did not affect the foam of the wines. These differences in proteolytic activity are confirmed by using BSA as a subs- trate: the Rhizopus culture degrades the 500 mg/L BSA in a few minutes, whereas the BSA degradation by the Botrytis culture remains considerably lower despite the longer culture of the fungus. Finally, the presence of epicatechin did not affect the wines’ foaming properties.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Richard Marchal¹, Candela Ruiz De Villa Sardón², Arnau Just Borràs², Nicolas Rozès², Fernando Zamora Marín², Joan-Miquel Canals Bosch², Thomas Salmon¹, José Francisco Cano Lira³, Jacques-Emmanuel Barbier4, Sabine Gognies¹

1. Université de Reims Champagne-Ardenne, Faculté des Sciences, Laboratoire d’Oenologie, 51687 Reims CEDEX 02, France
2. Universitat Rovira i Virgili, Facultat d’Enologia, Campus Sescelades, 43007 Tarragona, Spain
3. Un+iversitat Rovira i Virgili, Mycology, Environmental Microbiology Unit, Medicine Faculty / Oenology Faculty,  Sant Llorenç 21, 43201-Reus, Spain
4. Institut Œnologique de Champagne – ZI de Mardeuil – 51201 Épernay Cedex, France 

Contact the author*

Keywords

wine foam, Rhizopus, yeast proteins, aspartic protease

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

POTENTIAL OF PEPTIDASES FOR AVOIDING PROTEIN HAZES IN MUST AND WINE

Haze formation in wine during transportation and storage is an important issue for winemakers, since turbid wines are unacceptable for sale. Such haze often results from aggregation of unstable grape proteinaceous colloids. To date, foreseeably unstable wines need to be treated with bentonite to remove these, while excessive quantities, which are often required, affect the wine volume and quality (Cosme et al. 2020). One solution to avoid these drawbacks might be the use of peptidases. Marangon et al. (2012) reported that Aspergillopepsins I and II were able to hydrolyse the respective haze-relevant proteins in combination with a flash pasteurisation. In 2021, the OIV approved this enzymatic treatment for wine stabilisation (OIV-OENO 541A and 541B).

ALCOHOLIC FERMENTATION AND COLOR OF ROSÉ WINES: INVESTIGATIONS ON THE MECHANISMS RESPONSIBLE FOR SUCH DIVERSITY

Color is one of the key elements for the marketing of rosé wines due to their packaging in transparent bottles. Their broad color range is due to the presence of pigments belonging to phenolic compounds extracted from grapes or formed during the wine-making process. However, the mechanisms responsible for such diversity are poorly understood. The few investigations performed on rosé wines showed that their phenolic composition is highly variable, close to that of red wines for the darkest rosés but very different for light ones [1]. Moreover, large variations in the extent of color loss taking place during fermentation have been reported but the mechanisms involved and causes of such variability are unknown.

VALORIZATION OF GRAPE WINE POMACE USING PULSED ELECTRIC FIELDS (PEF) AND SUPERCRITICAL CO₂ (SC CO₂) EXTRACTION

Wine grape pomace quantitatively and qualitatively represents the most important fraction of wine waste. Namely, this by-product makes ~ 20% of the total mass of vinified grapes, and it is characterized with high concentrations of polyphenolic antioxidants, as well as grape seed oil. Hence, valorization of wine pomace, as an alternative to traditionally employed disposal, has drown considerable interest in recent years. Earlier studies were mostly focused on the extraction of phenolics, while mechanisms enhancing the extraction of lipid fraction from grape pomace, as well as their impact on the grape seed oil quality are far less investigated.

EUGENOL AS QUALITY MARKER OF WINES AND SPIRITS FROM HYBRID VINES: IMPACT OF DIFFERENT WINEMAKING AND DISTILLATION PROCESSES

Eugenol, widely spread in various plants notably cloves, basil and bay, was identified too in wines from hybrid grapes without contact with oak wood. This aromatic molecule presents a strong spicy note of clove and also antifongic properties. Eugenol was described as an endogenous compound of Baco blanc, from the grapes to the spirits of Armagnac area. Moreover, this compound is a chemical marker of Baco blanc products quality.
Influences of harvest time and different winemaking processes (settling, use of enzymatic preparations, lees content and stock time before distillation) on Baco blanc wine eugenol contents were explored using a two-levels full factorial Design of Experiments (DoEs).

EVALUATION OF INDIGENOUS SACCHAROMYCES CEREVISIAE ISOLATES FOR THEIR POTENTIAL USE AS FERMENTATION STARTERS IN ASSYRTIKO WINE

Assyrtiko is a rare ancient grape variety that constitutes one of the most popular in Greece. The objective of the current research was to evaluate indigenous Saccharomyces cerevisiae isolates as fermentation starters and also test the possible strain impact on volatile profile of Assyrtiko wine. 163 S. cerevisiae isolates, which were previously selected from spontaneous alcoholic fermentation, were identified at strain level by interdelta-PCR genomic fingerprinting. Yeasts strains were examined for their fermentative capacity in laboratory scale fermentation on pasteurized Assyrtiko grape must.