terclim by ICS banner
IVES 9 IVES Conference Series 9 IMPACT OF RHIZOPUS AND BOTRYTIS ON WINE FOAMING PROPERTIES

IMPACT OF RHIZOPUS AND BOTRYTIS ON WINE FOAMING PROPERTIES

Abstract

A lot of work has been done on the impact of Botrytis on the foam of sparkling wines. This work often concerns wines produced in cool regions, where Botrytis is the dominant fungal pathogen. However, in southern countries such as Spain, in particularly hot years such as 2022, the majority fungal pathogen is sometimes Rhizopus. Like Botrytis, Rhizopus is a fungus that produces an aspartic protease. The aim of this study was therefore to determine the foaming properties of wines produced with a synthetic must contaminated by a Rhizopus or Botrytis culture. In order to confirm the identification of the fungal strain, the D1-D3 domains of the 28S rRNA gene were amplified and sequenced. BLAST search indicated 100% identity with a reference strain of Rhizopus lyococcus (CBS 320.35).

The complete experimental design presents 12 modalities (AF in triplicate, i.e. 36 bottles). The fungal isolates of Botrytis cinerea (B. c.) and Rhizopus lyococcus (R. l.) were cultured using a modified version of the method described by Gimenez et al. (2022). Alcoholic fermentations (AF) were performed in 500mL glass bottles from synthetic grape must supplemented or not with 50 mg/L of epicatechin. The yeast strain S. cerevisiae Lalvin EC1118 (Lallemand) was used for the AF process. To examine the impact of the pathoge- nic fungi, 10% (v/v) of B.c. or R.l. culture were added (separately) to the model grape juice. Furthermore, two different concentrations of L-malic acid were added to the fermentation media creating two sets of conditions : 2g/L of L-malic acid (pH=3.5) and 6 g/L of L-malic acid (pH=3). The results of the wines with fungus were compared to those of the control wines obtained without fungus.

The results of this study show that the presence of Rhizopus in the must significantly or highly significantly degrades the foamability and foam stability of the wines (foam measured with the KRUSS DFA100 equipment). The analysis of the protein composition by SDS-PAGE clearly shows a degradation of the yeast proteins by the fungal proteases of Rhizopus. Surprisingly, the Botrytis strain used did not affect the foam of the wines. These differences in proteolytic activity are confirmed by using BSA as a subs- trate: the Rhizopus culture degrades the 500 mg/L BSA in a few minutes, whereas the BSA degradation by the Botrytis culture remains considerably lower despite the longer culture of the fungus. Finally, the presence of epicatechin did not affect the wines’ foaming properties.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Richard Marchal¹, Candela Ruiz De Villa Sardón², Arnau Just Borràs², Nicolas Rozès², Fernando Zamora Marín², Joan-Miquel Canals Bosch², Thomas Salmon¹, José Francisco Cano Lira³, Jacques-Emmanuel Barbier4, Sabine Gognies¹

1. Université de Reims Champagne-Ardenne, Faculté des Sciences, Laboratoire d’Oenologie, 51687 Reims CEDEX 02, France
2. Universitat Rovira i Virgili, Facultat d’Enologia, Campus Sescelades, 43007 Tarragona, Spain
3. Un+iversitat Rovira i Virgili, Mycology, Environmental Microbiology Unit, Medicine Faculty / Oenology Faculty,  Sant Llorenç 21, 43201-Reus, Spain
4. Institut Œnologique de Champagne – ZI de Mardeuil – 51201 Épernay Cedex, France 

Contact the author*

Keywords

wine foam, Rhizopus, yeast proteins, aspartic protease

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

Influence of agrophotovoltaic on vine and must in a cool climate

The current energy crisis means that interest in agrophotovoltaics has increased significantly. The reason behind this is that the system aims to combine agricultural production with energy production. During the three-year period from 2020 to 2022, the effects of photovoltaic panels on the vine, the yield and the quality of the must were studied in Walenstadt in northern Switzerland, an area with a cool, humid climate. 65 Pinot noir vines were planted in the 160m2 study area. Because of the large edge effects, only 3 repetitions with 4 vines each could be created. A significantly lower leaf infestation by Plasmopara viticola was observed among the panels in each of the three years.

UNTARGETED METABOLOMICS ANALYSES TO IDENTIFY A NEW SWEET COMPOUND RELEASED DURING POST-FERMENTATION MACERATION OF WINE

The gustatory balance of dry wines is centered on three flavors, sourness, bitterness and sweetness. Even if certain compounds were already identified as contributing to sweetness, some taste modifications remain largely unexplained1,2. Some empirical observations combined with sensory analyzes have shown that an increase of wine sweetness occurs during post-fermentation maceration³. This step is a key stage of red winemaking during which the juice is left in contact with the marc, that contains the solid parts of the grape (seeds, skins and sometimes stems). This work aimed to identify a new taste-active compound that contributes to this gain of sweetness.

PRODUCTION OF A FUNCTIONAL BEVERAGE FROM WINEMAKING BY-PRODUCTS: A NEW WAY OF VALORISATION

In the challenge of transforming waste into useful products that can be re-used in a circular economy perspective, winery by-products can be considered as a source of potentially bioactive molecules such as polyphenols. The wine industry generates each year 20 million tons of by-products. Kombucha fermentation is an ancestral process which allow to increase the biological properties of tea by the action of a microbial consortium formed by yeasts and bacteria called SCOBY. It belongs to the field of healthy food for which the interest of consumers is growing. The objective of this work was to propose a new functional beverage made from winemaking by-products fermented by a Kombucha SCOBY.

PROBING GRAPEVINE-BOTRYTIS CINEREA INTERACTION THROUGH MASS SPECTROMETRY IMAGING

Plants in their natural environment are in continuous interaction with large numbers of potentially pathogenic and beneficial microorganisms. Depending on the microbe, plants have evolved a variety of resistance mechanisms that can be constitutively expressed or induced. Phytoalexins, which are biocidal compounds of low to medium molecular weight synthesized by and accumulated in plants as a response to stress, take part in this intricate defense system.1,2
One of the limitations of our knowledge of phytoalexins is the difficulty of analyzing their spatial responsiveness occurring during plant- pathogen interactions under natural conditions.

EFFECT OF MICRO-OXYGENATION IN COLOR OF WINES MADE WITH TOASTED VINE-SHOOTS

The use of toasted vine-shoots (SEGs) as an enological tool is a new practice that seeks to improve wines, differentiating them and encouraging sustainable wine production. The micro-oxygenation (MOX) technique is normally combined with alternative oak products with the aim to simulate the oxygen transmission rate that takes place during the traditional barrel aging. Such new use for SEGs implies a reduction in color due to the absorption by the wood of the responsible compounds, therefore, given the known effect that MOX has shown to have on the modification of wine color, its use together with the SEGs could result in an interesting implementation with the aim to obtain final wines with more stable color over time.