terclim by ICS banner
IVES 9 IVES Conference Series 9 OTA DEGRADATION BY BACTERIAL LACCASEST

OTA DEGRADATION BY BACTERIAL LACCASEST

Abstract

Laccases from lactic acid bacteria (LAB) are described as multicopper oxidase enzymes with copper union sites. Among their applications, phenolic compounds’ oxidation and biogenic amines’ degrada-tion, have been described. Besides, the role of LAB in the toxicity reduction of ochratoxin A (OTA) has been reported (Fuchs et al., 2008; Luz et al., 2018). Fungal laccases, but not bacterial laccases, have been screened for OTA and mycotoxins’ degradation (Loi et al., 2018). OTA is a mycotoxin produced by some fungal species, such as Penicillium and Aspergillus sp., which infect grape bunches used for winemaking. OTA degradation is paramount given that it has been described as human-health harmful according to EFSA.

The work aimed to evaluate the OTA degrading capacity of three heterologous LAB laccases expressed in E. coli. The experimental procedure consisted on testing bacterial laccases from L. lactis, L. paracasei and P. parvulus in acetate buffer pH 4 with or without CuSO4 and OTA in presence and absence of several concentrations of epicatequin and complete polyphenolic extracts from red and white wine as media-tors. Degradation of OTA was followed and quantified by analyzing samples with HPLC-QToF-MS.

According to the results, OTA degradation in the reaction buffer with copper was at least three times higher than without copper. In addition, 0.75 mM epicatequin was the optimum concentration to obtain the highest OTA degradation with L. paracasei laccase (78%). Then, P. parvulus and L. lactis laccases were tested at this concentration, averaging 70% degradation. Finally, mean values of 40% and 10% OTA de-gradation were revealed when using polyphenolic extracts from red and white wine, respectively, for the three laccases. The application of these LAB laccases on OTA degradation in real wine needs to be further explored.

 

1. Fuchs S., et al. (2008). Food Chem Toxicol; 46:1398-1407.
2. Loi M., et al. (2018). Food Control; 90: 401-406.
3. Luz C., et al. (2018). Food Chem Toxicol; 112: 60-66.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Isaac Monroy¹, Isabel Pardo¹, Sergi Ferrer¹, José Pérez-Navarro², Sergio Gómez-Alonso²

1. ENOLAB, Institute BIOTECMED and Microbiology and Ecology Dept, University of Valencia
2. IRICA, University of Castilla-La Mancha

Contact the author*

Keywords

Ochratoxin A reduction, lactic acid bacteria laccases, polyphenolic compounds, redox media-tors

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

FACTORS AFFECTING QUERCETIN SOLUBILITY IN SANGIOVESE RED WINE: FIRST RESULTS

Quercetin (Q) is present in grape in form of glycosides and as aglycone. These compounds are extracted from grape skins during winemaking. In wines, following the hydrolysis reactions, the amount of quercetin aglycon can exceed its solubility value. Unfortunately, a threshold solubility concentration for quercetin in wine is not easy to determine because it depends on wine matrix (Gambuti et al., 2020).

OENOLOGICAL TANNINS FOR PREVENTING THE LIGHT-STRUCK TASTE IN WHITE AND ROSÉ WINES

The light exposure of wine can be detrimental as a relevant loss of aromas takes place [1] and light-induced reactions can occur. The latter involves riboflavin (RF), a photosensitive compound, that is fully reduced by acquiring two electrons. When the electron-donor is methionine, the light-struck taste (LST) can appear leading to cooked cabbage, onion and garlic odours-like [2]. The use of oenological tannins can limit the appearance of LST in both model wine [3] and white wine [4]. This research aimed to evaluate the impact of certain oenological tannins, selected in a previous study as the most effective against LST [5], in both white and rosé wines.

TANNINS AND ANTHOCYANINS KINETICS OF EXTRACTION FROM ARINARNOA, MARSELAN AND TANNAT UNDER DIFFERENT WINEMAKING TECHNIQUES

Marselan wines have an unusual high proportion of seed derived tannins from grapes having high proportions of skins, which are rich in tannins. But the causes behind this characteristic have not yet been identified. In vintage 2023 wines were made at experimental scale (9 kg by experimental unit) from Arinarnoa, Marselan and Tannat Vitis vinifera grape cultivars by traditional maceration, and by techniques aimed to increase the wine content in skin derived tannin: addition of extraction enzymes, addition at vatting of grape-skin enological tannins, or by extended maceration, known to increase the seed derived tannin contents of wines.

REMEDIATION OF SMOKE TAINTED WINE USING MOLECULARLY IMPRINTED POLYMERS

In recent years, vineyards in Australia, the US, Canada, Chile, South Africa and Europe have been exposed to smoke from wildfires. Wines made from smoke-affected grapes often exhibit unpleasant smoky, ashy characters, attributed to the presence of smoke-derived volatile compounds, including volatile phenols (which occur in free and glycosylated forms). Various strategies for remediation of smoke tainted wine have been evaluated. The most effective strategies involve the removal of smoke taint compounds via the addition of adsorbent materials such as activated carbon, which can either be added directly or used in combination with nanofiltration. However, these treatments often simultaneously remove wine constituents responsible for desirable aroma, flavour and colour attributes.

NEAR INFRARED SPECTROSCOPY FOR THE ESTIMATION OF TEMPRANILLO BLANCO VOLATILE COMPOSITION ALONG GRAPE MATURATION

Grape volatile compounds are mainly responsible for wine aroma, so it is important to know the va-rietal aromatic composition throughout ripening process. Currently, there are no tools that allow mea-suring the aromatic composition of grapes, in intact berries and periodically, throughout ripening, in the vineyard or in the winery. For this reason, this work evaluated the use of near infrared spectroscopy (NIR) to estimate the aromatic composition and total soluble solids (TSS) of Tempranillo Blanco berries during ripening. For this purpose, NIR spectra (1100-2100 nm) were acquired from 240 samples of in-tact berries, collected at different dates, from veraison to overripening.