terclim by ICS banner
IVES 9 IVES Conference Series 9 OTA DEGRADATION BY BACTERIAL LACCASEST

OTA DEGRADATION BY BACTERIAL LACCASEST

Abstract

Laccases from lactic acid bacteria (LAB) are described as multicopper oxidase enzymes with copper union sites. Among their applications, phenolic compounds’ oxidation and biogenic amines’ degrada-tion, have been described. Besides, the role of LAB in the toxicity reduction of ochratoxin A (OTA) has been reported (Fuchs et al., 2008; Luz et al., 2018). Fungal laccases, but not bacterial laccases, have been screened for OTA and mycotoxins’ degradation (Loi et al., 2018). OTA is a mycotoxin produced by some fungal species, such as Penicillium and Aspergillus sp., which infect grape bunches used for winemaking. OTA degradation is paramount given that it has been described as human-health harmful according to EFSA.

The work aimed to evaluate the OTA degrading capacity of three heterologous LAB laccases expressed in E. coli. The experimental procedure consisted on testing bacterial laccases from L. lactis, L. paracasei and P. parvulus in acetate buffer pH 4 with or without CuSO4 and OTA in presence and absence of several concentrations of epicatequin and complete polyphenolic extracts from red and white wine as media-tors. Degradation of OTA was followed and quantified by analyzing samples with HPLC-QToF-MS.

According to the results, OTA degradation in the reaction buffer with copper was at least three times higher than without copper. In addition, 0.75 mM epicatequin was the optimum concentration to obtain the highest OTA degradation with L. paracasei laccase (78%). Then, P. parvulus and L. lactis laccases were tested at this concentration, averaging 70% degradation. Finally, mean values of 40% and 10% OTA de-gradation were revealed when using polyphenolic extracts from red and white wine, respectively, for the three laccases. The application of these LAB laccases on OTA degradation in real wine needs to be further explored.

 

1. Fuchs S., et al. (2008). Food Chem Toxicol; 46:1398-1407.
2. Loi M., et al. (2018). Food Control; 90: 401-406.
3. Luz C., et al. (2018). Food Chem Toxicol; 112: 60-66.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Isaac Monroy¹, Isabel Pardo¹, Sergi Ferrer¹, José Pérez-Navarro², Sergio Gómez-Alonso²

1. ENOLAB, Institute BIOTECMED and Microbiology and Ecology Dept, University of Valencia
2. IRICA, University of Castilla-La Mancha

Contact the author*

Keywords

Ochratoxin A reduction, lactic acid bacteria laccases, polyphenolic compounds, redox media-tors

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

PHENOLICS DYNAMICS OF BERRIES FROM VITIS VINIFERA CV SYRAH GRAFTED ON TWO CONTRASTING ROOTSTOCKS UNDER COMBINED SALINITY AND WATER STRESSORS AND ITS EFFECT ON WINE QUALITY

Wine regions are getting warmer as average temperatures continue raising affecting grape growth, berry composition and wine production. Berry quality was evaluated in plants of Vitis vinifera cv Syrah grafted on two rootstocks, Paulsen (PL1103) and SO4, and grown under two salinity concentrations (LS:0.7dS/m and HS:2.5dSm-1) in combination with two irrigation regimes (HW:133% and CW:100%), being the seasonal water application 483mm (control, 100%). Spectrophotometer measurements from berry skin during veraison and harvest stages and from “young” wine samples, were indicative of the stressors effect and the mediation of the rootstocks. At veraison (i) total phenolics content were high under LSHW (0.7dSm-1 and high water conditions) for SO4 and PL1103.

MOUSY OFF-FLAVOURS IN WINES: UNVEILING THE MICROORGANISMS BEHIND IT

Taints and off-flavours are one of the major concerns in the wine industry and even if the issues provoked by them are harmless, they can still have a negative impact on the quality or on the visual perception of the consumer. Nowadays, the frequency of occurrence of mousy off-flavours in wines has increased.
The reasons behind this could be the significant decrease in sulphur dioxide addition during processing, the increase in pH or even the trend for spontaneous fermentation in wine. This off-flavour is associated with Brettanomyces bruxellensis or some lactic acid bacteria metabolisms.

BORDEAUX RED WINES WITHOUT ADDED SULFITES SPECIFICITIES: COMPOSITIONAL AND SENSORY APPROACHES TOWARDS HIGHLIGHTING AND EXPLAI-NING THEIR SPECIFIC FRUITINESS AND COOLNESS

With the development of naturality expectations, wines produced without any addition of sulfur dioxide (SO₂) become very popular for consumers and such wines are increasingly present on the market. Recent studies also showed that Bordeaux red wines without added SO₂ could be differentiated from a sensory point of view from similar wines produced with SO₂¹. Thus, the aim of the current study was to characterize from a sensory point of view, specific aromas of wines without added SO₂ and to identify compounds involved.

NEW INSIGHTS INTO VOLATILE SULPHUR COMPOUNDS SCALPING ON MICROAGGLOMERATED WINE CLOSURES

The evolution of wine during bottle ageing has been of great interest to ensure consistent quality over time. While the role of wine closures on the amount of oxygen is well-known [1], closures could also play other roles such as the scalping phenomenon of flavour compounds. Flavour scalping has been described as the sorption of flavour compounds by the packaging material, which could result in losses of flavour intensity. It has been reported in the literature that volatile sulphur compounds (VSC) can be scalped on wine closures depending on the type of closure (traditional and agglomerated cork, screw-cap, synthetic [2]).

PROGRESS OF STUDIES OF LEES ORIGINATING FROM THE FIRST ALCOHOLIC FERMENTATION OF CHAMPAGNE WINES

Champagne wines are produced via a two-step process: the first is an initial alcoholic fermentation of grape must that produces a still base wine, followed by a second fermentation in bottle – the prise de mousse – that produces the effervescence. This appellation produces non-vintage sparkling wines composed of still base wines assembled from different vintages, varieties, and regions. These base wines, or “reserve wines,” are typically conserved on their fine lies and used to compensate for quality variance between vintages (1). Continuously blending small amounts of these reserve wines into newer ones also facilitates preserving the producer’s “house style.”