terclim by ICS banner
IVES 9 IVES Conference Series 9 OTA DEGRADATION BY BACTERIAL LACCASEST

OTA DEGRADATION BY BACTERIAL LACCASEST

Abstract

Laccases from lactic acid bacteria (LAB) are described as multicopper oxidase enzymes with copper union sites. Among their applications, phenolic compounds’ oxidation and biogenic amines’ degrada-tion, have been described. Besides, the role of LAB in the toxicity reduction of ochratoxin A (OTA) has been reported (Fuchs et al., 2008; Luz et al., 2018). Fungal laccases, but not bacterial laccases, have been screened for OTA and mycotoxins’ degradation (Loi et al., 2018). OTA is a mycotoxin produced by some fungal species, such as Penicillium and Aspergillus sp., which infect grape bunches used for winemaking. OTA degradation is paramount given that it has been described as human-health harmful according to EFSA.

The work aimed to evaluate the OTA degrading capacity of three heterologous LAB laccases expressed in E. coli. The experimental procedure consisted on testing bacterial laccases from L. lactis, L. paracasei and P. parvulus in acetate buffer pH 4 with or without CuSO4 and OTA in presence and absence of several concentrations of epicatequin and complete polyphenolic extracts from red and white wine as media-tors. Degradation of OTA was followed and quantified by analyzing samples with HPLC-QToF-MS.

According to the results, OTA degradation in the reaction buffer with copper was at least three times higher than without copper. In addition, 0.75 mM epicatequin was the optimum concentration to obtain the highest OTA degradation with L. paracasei laccase (78%). Then, P. parvulus and L. lactis laccases were tested at this concentration, averaging 70% degradation. Finally, mean values of 40% and 10% OTA de-gradation were revealed when using polyphenolic extracts from red and white wine, respectively, for the three laccases. The application of these LAB laccases on OTA degradation in real wine needs to be further explored.

 

1. Fuchs S., et al. (2008). Food Chem Toxicol; 46:1398-1407.
2. Loi M., et al. (2018). Food Control; 90: 401-406.
3. Luz C., et al. (2018). Food Chem Toxicol; 112: 60-66.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Isaac Monroy¹, Isabel Pardo¹, Sergi Ferrer¹, José Pérez-Navarro², Sergio Gómez-Alonso²

1. ENOLAB, Institute BIOTECMED and Microbiology and Ecology Dept, University of Valencia
2. IRICA, University of Castilla-La Mancha

Contact the author*

Keywords

Ochratoxin A reduction, lactic acid bacteria laccases, polyphenolic compounds, redox media-tors

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

DOES LIGNIN AN ACCEPTABLE MARKER OF GRAPESEED MATURATION AND QUALITY?

Usually the winemaker consider polyphenols from the grape berry as an actor of the wine quality. There are frequently consider as a marker of grape maturity. It is commonly known that winemaker consider tannins and anthocyanins as main polyphenol actors for winemaking practices and wine quality. Here we will focus on the characterisation of lignins in grape seeds. Previous studies suggest that the seed is lignified [1], which could explain the change in colour of the seed when it reaches maturity and thus provide a reliable indicator for describing the maturity stage in the seed.

OPTIMIZING THE IDENTIFICATION OF NEW THIOLS AT TRACE LEVEL IN AGED RED WINES USING NEW OAK WOOD FUNCTIONALISATION STRATEGY

During bottle aging, many thiol compounds are involved in the expression of bouquet of great aged red wines according to the quality of the closure.1,2 Identifying thiol compounds in red wines is a challenging task due several drawbacks including, the complexity of the matrix, the low concentration of these impact compounds and the amount of wine needed.3,4
This work aims to develop a new strategy based on the functionalisation of oak wood organic extracts with H₂S, to produce new thiols, in order to mimic what can happen in red wine during bottle aging. Following this approach and through sensory analysis experiments, we demonstrated that the vanilla-like aroma of fresh oak wood was transformed into intense “meaty” nuances similar to those found in old but non oxidized red wines.

INSIGHTS ON THE ROLE OF GENES ON AROMA FORMATION OF WINES

Yeast secondary metabolism is a complex network of biochemical pathways and the genetic profile of the yeast carrying out the alcoholic fermentation is obviously important in the formation of the metabolites conferring specific odors to wine. The aim of the present research was to investigate the relative expression of genes involved in flavor compound production in eight different Saccharomyces cerevisiae strains.
Two commercial yeast strains Sc1 (S.cerevisiae x S.bayanus) and Sc2 (S.cerevisiae) and six indigenous S. cerevisiae strains (Sc3, Sc4, Sc5, Sc6, Sc7, Sc8) isolated during spontaneous fermentations were inoculated in Assyrtiko and Vidiano grape must.

UNCOVERING THE ROLE OF BERRY MATURITY STAGE AND GRAPE GENOTYPE ON WINE CHARACTERISTICS: INSIGHTS FROM CHEMICAL CHARACTERISTICS AND VOLATILE COMPOUNDS ANALYSIS

In a climate change context and aiming for sustainable, high-quality Bordeaux wine production, this project examines the impact of grape maturity levels in various cultivars chosen for their adaptability, genetic diversity, and potential to enhance wine quality. The study explores the effects on wine compo-sition and quality through sensory and molecular methods. We studied eight 14-year-old Vitis vinifera cv. grape varieties from the same area (VITADAPT plots 1 and 5): Cabernet Franc, Cabernet Sauvignon, Carmenère, Castets, Cot, Merlot, Petit Verdot, and Touriga Nacional.

Searching for the sweet spot: a focus on wine dealcoholization

It is well known that the vinification of grapes at full maturation can produce rich, full-bodied wines,
with intense and complex flavour profiles. However, the juice obtained from such grapes may have very
high sugar concentration, resulting in wines with an excessive concentration of ethanol. In addition, the decoupling between technological maturity and phenolic/aromatic one due to global warming, exacerbates this problem in some wine-growing regions. In parallel with the increase of the mean alcohol content of wines on the market, also the demand for reduced alcohol beverages has increased in recent years, mainly as a result of health and social concerns about the risks related to the consumption of alcohol.