terclim by ICS banner
IVES 9 IVES Conference Series 9 VOLATILE COMPOSITION OF WINES USING A GC/TOFMS: HS-SPME VS MICRO LLE AS SAMPLE PREPARATION METHODOLOGY

VOLATILE COMPOSITION OF WINES USING A GC/TOFMS: HS-SPME VS MICRO LLE AS SAMPLE PREPARATION METHODOLOGY

Abstract

Wine aroma analysis can be done by sensorial or instrumental analysis, the latter involving several methodologies based on olfactometric detection, electronic noses or gas chromatography. Gas Chromato-graphy has been widely used for the study of the volatile composition of wines and depending on the detection system coupled to the chromatographic system, quantification and identification of individual compounds can be achieved.

Prior to the chromatography, a sample preparation step is almost always required, but unfortunately there is no extraction procedure that can aid in the detection of the wide range of volatile compounds that exists in a wine sample. Wine volatile profile is characterized to have thousands of compounds with varying chemical properties, like molecular weight, structure, polarity and molecular structures. Moreover, they exist in a wide range of concentration, which, sometimes implies that a pre-concentration step is also required, if the ones existing in very low concentrations are of interest. As far as sample preparation methods for the analysis of wine aroma concerns, one can found thousands of bibliographic references, but the most used ones are probably the liquid-liquid extraction (LLE) and the solid-phase microextraction (SPME). Extensive reviews on the different sample preparation methods that has been used for wine analysis, along with each one advantages and drawbacks, has already received researcher’s attention (Costa Freitas et al, 2012)

In light of the above, this work intents to discuss the use of two different sample preparation methods to quantify and identify volatile compounds in wines.

Two sample preparation methods were compared: a micro liquid-liquid extraction with 500mL of dichloromethane (based on Vilanova et al, 2010) and a HS-SPME (based on Pereira et al 2021). Chromatographic method was the same for both sample preparation method.

The number of compounds identified by HS-SPME was higher than the ones identified by micro-LLE. 26 compounds were identified in wines by both sample preparation methods. Since the majority of com-pounds identified by each sample preparation methodologies are different, choose to do one or another, or even both, should be taken into consideration when the goal is to go deep on volatile composition of wines.

 

1. M. Costa Freitas; M. D. R. Gomes da Silva; M. J. Cabrita (2012) “Sampling and sample preparation techniques for the determination of volatile components in grape juice, wine and alcoholic beverages” In Comprehensive Sampling and Sample Preparation. Volume 4, Pawliszyn J., Mondello L., Dugo P. Eds; Elsevier, Academic Press: Oxford, UK, pp 27–41, 2012. ISBN: 9780123813732
2. Singleton, V. e Rossi, J. (1965) Colorimetry of Total Phenolic Compounds with Phosphomolybdic-Phosphotungstic Acid Reagents. American Journal of Enology and Viticultura, 16, 144-158.
3. Mar Vilanova, Zlatina Genisheva, Antón Masa, José Maria Oliveira (2010). Correlation between volatile composition and sensory properties in Spanish Albariño wines. Microchemical Journal, 95, 240-246.
4. Pereira, C., Mendes, D., Dias, T., Garcia, R., da Silva, M. and Cabrita, M., 2021. Revealing the yeast modulation potential on amino acid composition and volatile profile of Arinto white wines by a combined chromatographic-based approach. Journal of Chromatography A, 1641, p.461991.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Nuno Martins¹, Maria João Cabrita1,2 Raquel Garcia1,2

1. MED—Mediterranean Institute for Agriculture, Environment and Development & CHANGE—Global Change and Sustainabi-lity Institute, Universidade de Évora, Pólo da Mitra, Ap. 94, 7006-554 Évora, Portugal
2. Departamento de Fitotecnia, Escola de Ciências e Tecnologia, Universidade de Évora, Pólo da Mitra, Ap. 94, 7006-554 Évora, Portugal

Contact the author*

Keywords

red wine, volatiles, sample preparation, GC/TOFMS

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

LARGE-SCALE PHENOTYPIC SCREENING OF THE SPOILAGE YEAST BRETTANOMYCES BRUXELLENSIS: UNTANGLING PATTERNS OF ADAPTATION AND SELECTION, AND CONSEQUENCES FOR INNOVATIVE WINE TREATMENTS

Brettanomyces bruxellensis is considered as the main spoilage yeast in oenology. Its presence in red wine leads to off-flavour due to the production of volatile phenols such as 4-vinylphenol, 4-vinylguaiacol, 4-ethylphenol and 4-ethylguaiacol, whose aromatic notes are unpleasant (e.g. animal, leather, horse or pharmaceutical). Beside wine, B. bruxellensis is commonly isolated from beer, kombucha and bioethanol production, where its role can be described as negative or positive. Recent genomic studies unveiled the existence of various populations.

Beyond liking scores: the importance of the drinking experience to understand our consumers

The presentation will approach the understanding of wine consumers´ perception based on the experiential model suggested by Warell (2008). In this framework, wine consumption gives rise to a
variety of experiences related to the perception, understanding, and judgment of the product. These
multidimensional facets of the drinking experience can be explored by measuring affective, cognitive,
and sensory responses of consumers, which are shown to be stable regardless of the social context.

PHOTO OXIDATION OF LUGANA WINES: INFLUENCE OF YEASTS AND RESIDUAL NITROGEN ON VSCS PROFILE

Lugana wines are made from Turbiana grapes. In recent times, many white and rosé wines are bottled and stored in flint glass bottles because of commercial appeal. However, this practice could worsen the aroma profile of the wine, especially as regards the development of volatile sulfur compounds (VSCs). This study aims to investigate the consequences of exposure to light in flint bottles on VSCs profile of Lugana wines fermented with two different yeasts and with different post-fermentation residual nitrogen.

PRODUCTION OF A FUNCTIONAL BEVERAGE FROM WINEMAKING BY-PRODUCTS: A NEW WAY OF VALORISATION

In the challenge of transforming waste into useful products that can be re-used in a circular economy perspective, winery by-products can be considered as a source of potentially bioactive molecules such as polyphenols. The wine industry generates each year 20 million tons of by-products. Kombucha fermentation is an ancestral process which allow to increase the biological properties of tea by the action of a microbial consortium formed by yeasts and bacteria called SCOBY. It belongs to the field of healthy food for which the interest of consumers is growing. The objective of this work was to propose a new functional beverage made from winemaking by-products fermented by a Kombucha SCOBY.

REMEDIATION OF SMOKE TAINTED WINE USING MOLECULARLY IMPRINTED POLYMERS

In recent years, vineyards in Australia, the US, Canada, Chile, South Africa and Europe have been exposed to smoke from wildfires. Wines made from smoke-affected grapes often exhibit unpleasant smoky, ashy characters, attributed to the presence of smoke-derived volatile compounds, including volatile phenols (which occur in free and glycosylated forms). Various strategies for remediation of smoke tainted wine have been evaluated. The most effective strategies involve the removal of smoke taint compounds via the addition of adsorbent materials such as activated carbon, which can either be added directly or used in combination with nanofiltration. However, these treatments often simultaneously remove wine constituents responsible for desirable aroma, flavour and colour attributes.