terclim by ICS banner
IVES 9 IVES Conference Series 9 ANTHOCYANINS EXTRACTION FROM GRAPE POMACE USING EUTECTIC SOLVENTS

ANTHOCYANINS EXTRACTION FROM GRAPE POMACE USING EUTECTIC SOLVENTS

Abstract

Grape pomace is one of the main by-products generated after pressing in winemaking.Emerging methods, such as ultrasound-assisted extraction with eutectic mixtures, have great potential due to their low toxicity, and high biodegradability. Choline chloride (ChCl) was used as a hydrogen bond acceptor and its corresponding hydrogen bond donor (malic acid, citric acid, and glycerol: urea). Components were heated at 80 °C and stirred until a clear liquid was obtained. Distilled water was added (30 % v/v). A solid-liquid ratio of 1 g pomace per 10 ml of eutectic solvent was used. Total anthocyanins were determined. Malvidin-3-O-coumaroylglucoside was quantified by HPLC. Metabisulfite bleaching and the viscosity of the extracts were also determined. The highest extraction was obtained for the methanol/water system.The eutectic system that showed the highest extraction was the mixture of choline chloride, urea, and glycerol in a molar ratio of 1:1:1. Glycerol is classified as a polyol. It can modify the polarity of water so it can be used as a co-solvent in the extraction of polyphenols. In addition, it is considered a highly flexible molecule, capable of forming intra- and intermolecular hydrogen bonds [1]. The higher extraction of choline chloride glycerol and urea (molar ratio 1:1:1) could be due to the influence of the lower polarity of glycerol presenting a higher affinity, probably with malvidin-3-O-coumaroylglucoside, which is less polar than Mv-3-O-glc. In HPLC analysis, malvidin-3-O-coumaroylglucoside was the main anthocyanin identified in all extracts.In eutectic mixtures, viscosity is the property that limits the extraction process compared to conventional solvent extractions. The extract obtained with the choline chloride: urea: glycerol (1:2:2) system had the lowest viscosity values, while the rest of the extracts presented higher viscosities. Viscosity reflects how compact a molecular structure is. Therefore, it can be inferred that the systems with malic acid and citric acid with choline chloride in molar ratios 1:2 present a compact molecular structure with a minimum of holes, which results in less diffusion during the extraction process. The choline chloride: malic acid (1:1) system presented significant resistance to sulfite bleaching at pH 3.5, losing approximately 34 % of color. The choline chloride: urea: glycerol (1:1:1) system lost approximately 50 % of the color, presenting a lower resistance to discoloration.

1. A. P. Abbott, R. C. Harris, K. S. Ryder, C. D’Agostino, L. F. Gladden, and M. D. Mantle, “Glycerol eutectics as sustainable solvent systems,” Green Chem., vol. 13, no. 1, pp. 82–90, 2011

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Lilisbet Castellanos-Gallo¹, Lourdes Ballinas-Casarrubias¹, Jose-Carlos Espinoza-Hicks¹,  Johan Mendo-Za-Chacón¹, León Hernandez-Ochoa¹

1. Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Ciudad Universitaria s/n, C.P. 31170 Chihuahua Mexico

Contact the author*

Keywords

Extraction, malvidin-3-O-coumaroylglucoside, Eutectic solvents, Grape pomace

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

VOLATILE COMPOSITION OF WINES USING A GC/TOFMS: HS-SPME VS MICRO LLE AS SAMPLE PREPARATION METHODOLOGY

Wine aroma analysis can be done by sensorial or instrumental analysis, the latter involving several me-thodologies based on olfactometric detection, electronic noses or gas chromatography. Gas Chromatography has been widely used for the study of the volatile composition of wines and depending on the detection system coupled to the chromatographic system, quantification and identification of individual compounds can be achieved.

THE EFFECT OF DIFFERENT TERROIRS ON AROMA COMPOUNDS OF ‘KALECIK KARASI’ WINES

Kalecik Karası is a domestic grape variety of Turkey, originating from Kalecik district, 80 km from Ankara. Although there is no definite evidence, it is known that it was used in wine production by many civilizations that lived in the Anatolian region, especially the Hittites. Compared to other black wine grapes, it stands out with its low tannin content, rich fruity aroma and complex structure. In good vintages, red fruits such as strawberries, cherries and raspberries stand out in the aroma profile. Although its structure is elegant, it has the potential to age and develop similar to the ‘Pinot Noir’ wine of the Burgundy region. This offers a complex aroma structure including red flowers, earth and ripe fruits.

CHEMICAL DRIVERS OF POSITIVE REDUCTION IN NEW ZEALAND CHARDONNAY WINES

According to winemakers, wine experts and sommeliers, aromas of wet stone, mineral, struck match and flint in white wines styles, such as those produced from Vitis vinifera L. cv. Chardonnay, are considered to be hallmarks of positive reduction.1,2 In recent years, the production of Chardonnay styles defined by aroma characteristics related to positive reduction has become more desirable among wine experts and consumers. The chemical basis of positive reduction is thought to originate from the concentration of specific volatile sulfur compounds (VSCs), including methanethiol (MeSH) imparting mineral and chalk notes,3 and benzenemethanethiol (BMT) responsible for struck match and flint.1,4

FUNGAL DIVERSITY AND DYNAMICS IN CHAMPAGNE VINEYARDS: FROM VINE TO WINE

Champagne is a well-known wine region in Northern France with distinct terroirs and three main grape varieties. As for any vineyard, wine quality is highly linked to the microbiological characteristics of the raw materials. However, Champagne grape microbiota, especially its fungal component, has yet to be fully characterized. Our study focused on describing this mycobiota, from vine to small scale model wine, for the two main Champagne grape varieties, Pinot Noir and Meunier, using complementary cultural and omics approaches.

WHICH IMPACT FOR PROANTHOCYANIDIC TANNINS ON RED WINE FRUITY AROMA? SENSORY AND PHYSICOCHEMICAL APPROACHES

Previous research on the fruity character of red wines highlighted the role of esters. Literature provides evidence that, besides these esters, other compounds that are not necessarily volatiles may have an important impact on the overall aroma of wine, contributing to a modulation of its global aromatic expression. The goal of this work was to assess the olfactory consequences of a mixture between esters and proanthocyanidic tannins, through sensory and physico-chemical approaches.
Sensory analysis of numerous aromatic reconstitutions, including triangular tests, detection thresholds, and sensory profiles, were conducted in order to evaluate the sensory impact of tannins on red wine esters perception.