terclim by ICS banner
IVES 9 IVES Conference Series 9 ANTHOCYANINS EXTRACTION FROM GRAPE POMACE USING EUTECTIC SOLVENTS

ANTHOCYANINS EXTRACTION FROM GRAPE POMACE USING EUTECTIC SOLVENTS

Abstract

Grape pomace is one of the main by-products generated after pressing in winemaking.Emerging methods, such as ultrasound-assisted extraction with eutectic mixtures, have great potential due to their low toxicity, and high biodegradability. Choline chloride (ChCl) was used as a hydrogen bond acceptor and its corresponding hydrogen bond donor (malic acid, citric acid, and glycerol: urea). Components were heated at 80 °C and stirred until a clear liquid was obtained. Distilled water was added (30 % v/v). A solid-liquid ratio of 1 g pomace per 10 ml of eutectic solvent was used. Total anthocyanins were determined. Malvidin-3-O-coumaroylglucoside was quantified by HPLC. Metabisulfite bleaching and the viscosity of the extracts were also determined. The highest extraction was obtained for the methanol/water system.The eutectic system that showed the highest extraction was the mixture of choline chloride, urea, and glycerol in a molar ratio of 1:1:1. Glycerol is classified as a polyol. It can modify the polarity of water so it can be used as a co-solvent in the extraction of polyphenols. In addition, it is considered a highly flexible molecule, capable of forming intra- and intermolecular hydrogen bonds [1]. The higher extraction of choline chloride glycerol and urea (molar ratio 1:1:1) could be due to the influence of the lower polarity of glycerol presenting a higher affinity, probably with malvidin-3-O-coumaroylglucoside, which is less polar than Mv-3-O-glc. In HPLC analysis, malvidin-3-O-coumaroylglucoside was the main anthocyanin identified in all extracts.In eutectic mixtures, viscosity is the property that limits the extraction process compared to conventional solvent extractions. The extract obtained with the choline chloride: urea: glycerol (1:2:2) system had the lowest viscosity values, while the rest of the extracts presented higher viscosities. Viscosity reflects how compact a molecular structure is. Therefore, it can be inferred that the systems with malic acid and citric acid with choline chloride in molar ratios 1:2 present a compact molecular structure with a minimum of holes, which results in less diffusion during the extraction process. The choline chloride: malic acid (1:1) system presented significant resistance to sulfite bleaching at pH 3.5, losing approximately 34 % of color. The choline chloride: urea: glycerol (1:1:1) system lost approximately 50 % of the color, presenting a lower resistance to discoloration.

1. A. P. Abbott, R. C. Harris, K. S. Ryder, C. D’Agostino, L. F. Gladden, and M. D. Mantle, “Glycerol eutectics as sustainable solvent systems,” Green Chem., vol. 13, no. 1, pp. 82–90, 2011

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Lilisbet Castellanos-Gallo¹, Lourdes Ballinas-Casarrubias¹, Jose-Carlos Espinoza-Hicks¹,  Johan Mendo-Za-Chacón¹, León Hernandez-Ochoa¹

1. Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Ciudad Universitaria s/n, C.P. 31170 Chihuahua Mexico

Contact the author*

Keywords

Extraction, malvidin-3-O-coumaroylglucoside, Eutectic solvents, Grape pomace

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

IMPACT OF FINING WITH K-CARRAGEENAN, BENTONITE, AND CHITOSAN ON PROTEIN STABILITY AND MACROMOLECULAR COMPOUNDS OF ALBARIÑO WHITE WINE PRODUCED WITH AND WITHOUT PRE-FERMENTATIVE SKIN MACERATION

Pre-fermentative skin maceration is a technique used in white wine production to enhance varietal aroma, but it can increase protein concentration, leading to protein instability and haze formation [1]. To prevent protein instability, wine producers typically use fining agents such as bentonite, before wine bottling, which can negatively impact sensory characteristics and produce waste [2,3]. The aim of this study was to understand the impact of alternative techniques such as the application of polysaccharides (k-carrageenan and chitosan) on protein stability and on the wine macromolecular composition.

TOWARDS THE SHELF-LIFE PREDICTION OF OLD CHAMPAGNE VINTAGES DEPENDING ON THE BOTTLE CAPACITY

Today, nearly one billion bottles of different sizes and capacities are aging in Champagne cellars while waiting to be put on the market. Among them, several tens of thousands of prestigious cuvees elaborated prior the 2000s are potentially concerned by prolonged aging on lees. However, when it comes to champagne tasting, dissolved CO₂ is a key compound responsible for the very much sought-after effer-vescence in glasses [1]. Yet, the slow decrease of dissolved CO₂ during prolonged aging of the most prestigious cuvees raises the issue of how long a champagne can age before it becomes unable to form CO₂ bubbles during tasting [2].

INVESTIGATION OF FILM COATINGS AS A PROTECTIVE LAYER IN REDUCING THE ABSORPTION OF SMOKE PHENOLS INTO PINOT NOIR GRAPES

Wine grapes exposed to wildfire smoke have resulted in wines with burnt and ashy sensory characteristics¹, that are undesirable qualities in wine. In extreme wildfire events, this can lead to total loss of grape crop. Currently there are no effective solutions in the market to prevent the uptake of smoke compounds into grapes. In this study, previously developed innovative film coatings were tested to analyze their effectiveness in reducing smoke phenol absorption². Four different cellulose nanofiber-based film types were investigated.

PROTEOMIC STUDY OF THE USE OF MANNOPROTEINS BY OENOCOCCUS OENI TO IMPROVE MALOLACTIC FERMENTATION

Malolactic fermentation (MLF) is a desired process to decrease acidity in wine. This fermentation, carried out mostly by Oenococcus oeni, is sometimes challenging due to the wine stress factors affecting this lactic acid bacterium. Wine is a harsh environment for microbial survival due to the presence of ethanol and the low pH, and with limited nutrients that compromise O. oeni development. This may result in slow or stuck fermentations. After the alcoholic fermentation the nutrients that remain in the medium, mainly released by yeast, can be used in a beneficial way by O. oeni during MLF.

WINE LEES AS A SOURCE OF NITROGEN FOR OENOCOCCUS OENI TO IMPROVE MALOLACTIC FERMENTATION PERFORMANCE

Malolactic fermentation (MLF) is a desired process in red and acidic white wines, after alcoholic fermentation (AF), carried out by the lactic acid bacterium (LAB) Oenococcus oeni. The advantages are an increase of pH, microbiological stabilization and organoleptic improvement of the final wine. However, the presence of stress factors such as ethanol, low pH, high total SO2, lack of nutrients and presence of inhibitors, could affect the successful completion of MLF [1]. Changes in amino acid composition and deficiencies in peptides after AF, showed that MLF can be delayed, signaling its importance for bacterial growth and L-malic acid degradation during MLF [2].