terclim by ICS banner
IVES 9 IVES Conference Series 9 ANTHOCYANINS EXTRACTION FROM GRAPE POMACE USING EUTECTIC SOLVENTS

ANTHOCYANINS EXTRACTION FROM GRAPE POMACE USING EUTECTIC SOLVENTS

Abstract

Grape pomace is one of the main by-products generated after pressing in winemaking.Emerging methods, such as ultrasound-assisted extraction with eutectic mixtures, have great potential due to their low toxicity, and high biodegradability. Choline chloride (ChCl) was used as a hydrogen bond acceptor and its corresponding hydrogen bond donor (malic acid, citric acid, and glycerol: urea). Components were heated at 80 °C and stirred until a clear liquid was obtained. Distilled water was added (30 % v/v). A solid-liquid ratio of 1 g pomace per 10 ml of eutectic solvent was used. Total anthocyanins were determined. Malvidin-3-O-coumaroylglucoside was quantified by HPLC. Metabisulfite bleaching and the viscosity of the extracts were also determined. The highest extraction was obtained for the methanol/water system.The eutectic system that showed the highest extraction was the mixture of choline chloride, urea, and glycerol in a molar ratio of 1:1:1. Glycerol is classified as a polyol. It can modify the polarity of water so it can be used as a co-solvent in the extraction of polyphenols. In addition, it is considered a highly flexible molecule, capable of forming intra- and intermolecular hydrogen bonds [1]. The higher extraction of choline chloride glycerol and urea (molar ratio 1:1:1) could be due to the influence of the lower polarity of glycerol presenting a higher affinity, probably with malvidin-3-O-coumaroylglucoside, which is less polar than Mv-3-O-glc. In HPLC analysis, malvidin-3-O-coumaroylglucoside was the main anthocyanin identified in all extracts.In eutectic mixtures, viscosity is the property that limits the extraction process compared to conventional solvent extractions. The extract obtained with the choline chloride: urea: glycerol (1:2:2) system had the lowest viscosity values, while the rest of the extracts presented higher viscosities. Viscosity reflects how compact a molecular structure is. Therefore, it can be inferred that the systems with malic acid and citric acid with choline chloride in molar ratios 1:2 present a compact molecular structure with a minimum of holes, which results in less diffusion during the extraction process. The choline chloride: malic acid (1:1) system presented significant resistance to sulfite bleaching at pH 3.5, losing approximately 34 % of color. The choline chloride: urea: glycerol (1:1:1) system lost approximately 50 % of the color, presenting a lower resistance to discoloration.

1. A. P. Abbott, R. C. Harris, K. S. Ryder, C. D’Agostino, L. F. Gladden, and M. D. Mantle, “Glycerol eutectics as sustainable solvent systems,” Green Chem., vol. 13, no. 1, pp. 82–90, 2011

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Lilisbet Castellanos-Gallo¹, Lourdes Ballinas-Casarrubias¹, Jose-Carlos Espinoza-Hicks¹,  Johan Mendo-Za-Chacón¹, León Hernandez-Ochoa¹

1. Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Ciudad Universitaria s/n, C.P. 31170 Chihuahua Mexico

Contact the author*

Keywords

Extraction, malvidin-3-O-coumaroylglucoside, Eutectic solvents, Grape pomace

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

SHIRAZ FLAVONOID EXTRACTABILITY IMPACTED BY HIGH AND EXTREME HIGH TEMPERATURES

Climate change is leading to an increase in average temperature and in the severity and occurrence of heatwaves, and is already disrupting grapevine phenology. In Australia, with the evolution of the weather of grape growing regions that are already warm and hot, berry composition including flavonoids, for which biosynthesis depends on bunch microclimate, are expected to be impacted [1]. These compounds, such as anthocyanins and tannins, contribute substantially to grape and wine quality. The goal of this research was to determine how flavonoid extraction is impacted when bunches are exposed to high (>35 °C) and extreme high (>45 °C) temperatures during berry development and maturity.

FUNCTIONALIZED MESOPOROUS SILICA IS A VIABLE ALTERNATIVE TO BENTONITE FOR WINE PROTEIN STABILIZATION

The presence of grape-derived heat unstable proteins can lead to haze formation in white wines [1], an instability prevented by removing these proteins by adding bentonite, a hydrated aluminum silicate that interacts electrostatically with wine proteins leading to their flocculation. Despite effective, using bentonite has several drawbacks as the costs associated with its use, the potential negative effects on wine quality, and its environmental impact, so that alternative solutions are needed.

CONSUMER PERCEPTION OF INTERSPECIFIC HYBRID RED WINE COLOR IN RELATION TO ANTHOCYANIN PROFILE AND CHEMICAL COLOR PARAMETERS

Interspecific hybrid winegrapes are of growing interest in the context of climate change based on their disease resistance and cold hardiness. In addition to a need for increased understanding of their chemical composition, there is little empirical evidence on the consumer perception of non-vinifera wine. Phenolic compounds, and particularly color, play an important organoleptic and quality determination role in wine, but can vary significantly in interspecific hybrid wines compared to wines produced from Vitis vinifera cultivars [1, 2, 3]. Anecdotally, the variation in anthocyanin species, interactions, and concentrations in interspecific hybrids could result in a variance from“vinifera-like” wine color.

THE EFFECT OF PRE-FERMENTATIVE GLYPHOSATE ADDITION ON THE METABOLITE PROFILE OF WINE

The synthetic herbicide glyphosate has been used extensively in viticulture over many decades to combat weeds. Despite this, the possible influence of residual glyphosate on both the alcoholic fermentation of grape juice and the subsequent metabolite profile of wines has not been investigated. In this study, Pinot noir juice supplemented with different concentrations of glyphosate (0 µg L-1, 10 µg L-1 and 1000 µg L-1) was fermented with commercial Saccharomyces cerevisiae yeast strains. Using a combination of analytical methods, 80 metabolites were quantified in the resulting wines.

EXPLORING RED WINE TYPICITY OF CORBIÈRES: EVALUATION OF THE DEGREE OF IMPACT OF VINIFICATION PROCESS ON THE CHEMICAL COMPOSITION AND ORGANOLEPTIC PROPERTIES OF WINES FROM DIFFERENT TERROIR

It is important nowadays for wine producers to create a product that is an expression of their terroir, a concept including the interaction between a place (topography, climate, soil), the people (tradition, winemaking and viticultural practices) and the resulting product (grape varieties, wines) [1]. Nonetheless, wine’s typicity linked to those terroirs must be easily recognizable by consumers thanks to distinctive sensory characters and composition [2]. Among the compounds of interest, aromatic compounds and polyphenols play an important role in the quality of red wines, by impacting on the odour, color and astringency. To explore the influence of terroir factors, including climate, soil and human practices, on the chemical and sensory profile of wines, red wines from five terroirs of the Corbières appellation were subjected to a general study approach.