terclim by ICS banner
IVES 9 IVES Conference Series 9 EFFECTS OF LEAF REMOVAL AT DIFFERENT BUNCHES PHENOLOGICAL STAGES ON FREE AND GLYCOCONJUGATE AROMAS OF SKINS AND PULPS OF TWO ITALIAN RED GRAPES

EFFECTS OF LEAF REMOVAL AT DIFFERENT BUNCHES PHENOLOGICAL STAGES ON FREE AND GLYCOCONJUGATE AROMAS OF SKINS AND PULPS OF TWO ITALIAN RED GRAPES

Abstract

Canopy-management practices are applied in viticulture to improve berries composition and quality, having a great impact on primary and secondary grape metabolism. Among these techniques, cluster zone leaf removal (defoliation) is widely used to manage air circulation, temperature and light radiation of grape bunches and close environment. Since volatiles are quantitatively and qualitatively influenced by the degree of fruit ripeness, the level of solar exposure, and the thermal environment in which grapes ripen, leaf removal has been shown to affect volatile composition of grape berries [1].

The aim of this research was to assess the impact of vine defoliation on free and glycosylated VOCs (Volatile Organic Compounds) of two Italian red grapes: Nebbiolo (neutral) and Aleatico (semi-aromatic). Defoliation was performed at fruit set phenological stage for Aleatico grapevines, and for Nebbiolo also at berries touch.

Solid Phase Extraction/Gas Chromatography–Mass Spectrometry (SPE/GC-MS) was carried out to ana-lyse free and glycoconjugates VOCs isolated from skins and pulps as separate portions of the berries [2].

The results showed that defoliation had an almost negligible effect on free and glycosylated volatiles of Aleatico grapes, thus suggesting that defoliation at fruit set did not change the volatile composition of this grape variety. A different behaviour was observed for Nebbiolo grapes, on both free and bound VOCs, with a greater impact on the first. Indeed, all the 30 free VOCs identified were significantly (ANOVA; p<0.05) affected by defoliation and by the time at which it was carried out, with a greater influence on the skin components. Early defoliation at fruit set did not favour the accumulation of free VOCs in Nebbiolo skins, significantly reducing the content of several VOCs, such as n-butyl acetate, terpenes (α-terpineol, and nerol) and aldehydes (hexanal, and 2-hexanal). On the other hand, late defoliation performed at berries touch, reduced n-butyl acetate, but increased alcohols content (i.e., 3-methyl-1-butanol, 1-pentanol, 2-ethyl-1-hexanol, benzyl and phenylethyl alcohols), nerol, aldehydes (i.e., 2-hexanal), and vanillin.

Results suggest that the effects of defoliation at fruit set on the VOCs pattern is cultivar dependent and almost ineffective on Aleatico grapes. Moreover, early defoliation at fruit-set seems stressful for Neb-biolo grapes and its odorous and potentially secondary metabolites. These results can be useful to improve canopy and winemaking precision practices.

 

1. Poni et al., 2006. DOI: 10.5344/ajev.2006.57.4.397
2. Piombino et al., 2022. DOI: 10.1111/ajgw.12521

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Paola Piombino¹, Elisabetta Pittari¹, Alessandro Genovese², Andrea Bellincontro³, Osvaldo Failla⁴, Luigi Moio1, Fabio Mencarelli⁵

1. Department of Agricultural Sciences, Division of Vine and Wine Sciences, University of Naples Federico II, Avellino 83100, Italy
2. Department of Agricultural Sciences, Division of Food Science and Technology, University of Naples Federico II, Portici (NA), 80055, Italy
3. DIBAF, University of Tuscia, Via De Lellis, 01100 Viterbo, Italy
4. Department of Agricultural and Environmental Sciences, University of Milan, 20133 Milano, Italy
5. Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto 80, Pisa 56124, Italy

Contact the author*

Keywords

defoliation, secondary metabolites, aromas

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

Microbial ecosystems in wineries – molecular interactions between species and modelling of population dynamics

Microbial ecosystems are primary drivers of viticultural, oenological and other cellar-related processes such as wastewater treatment. Metagenomic datasets have broadly mapped the vast microbial species diversity of many of the relevant ecological niches within the broader wine environment, from vineyard soils to plants and grapes to fermentation. The data highlight that species identities and diversity significantly impact agronomic performance of vineyards as well as wine quality, but the complexity of these systems and of microbial growth dynamics has defeated attempts to offer actionable tools to guide or predict specific outcomes of ecosystem-based interventions.

UNRAVELING THE CHEMICAL MECHANISM OF MND FORMATION IN RED WINE DURING BOTTLE AGING : IDENTIFICATION OF A NEW GLUCOSYLATED HYDROXYKETONE PRO-PRECURSOR

During bottle aging, the development of wine aroma through low and gradual oxygen exposure is often positive in red wines, but can be unfavorable in many cases, resulting in a rapid loss of fresh, fruity flavors. Prematurely aged wines are marked by intense prune and fig aromatic nuances that dominate the desirable bouquet achieved through aging (Pons et al., 2013). This aromatic defect, in part, is caused by the presence of 3-methyl-2,4-nonanedione (MND). MND content was shown to be lower in nonoxidized red wines and higher in oxidized red wines, which systematically exceeds the odor detection threshold (62 ng/L).

PINKING PHENOMENA ON WHITE WINES: RELATION BETWEEN PINKING SUSCEPTIBILITY INDEX (PSI) AND WINE ANTHOCYANINS CONTENT

Pinking is the emergence of pink tones in white wines exclusively produced from white grape varieties, known as pinking phenomena for many years. Pinking is essentially appeared when white wines are produced under reducing conditions [1,2,3]. Pinking usually occurs after bottling and storage of white wines, but its appearance has also been described after alcoholic fermentation or even as soon as the grape must is extracted [4]. Therefore, the purpose of this work was to investigate the existence of an-thocyanins in white wines made from different white grape varieties and grown locations and critically evaluate the most common method used for predicting pinking appearance in white wines: the Pinking Susceptibility Index (PSI).

TARTARIC STABILIZATION MAY AFFECT THE COLOR AND POLYPHENOLIC COMPOSITION OF TANNAT RED WINES FROM URUGUAY

Tartrate precipitation affects the properties of wines, due to the formation of crystals that cause turbidity, even after being bottled. The forced tartaric stabilization is carried out frequently for young wines, through various physicochemical procedures. The traditional treatment for tartaric stabilization is refrigeration, but it can have a negative effect on wine’s sensory properties, and particularly on the color of red wines. The aim of this study was to evaluate the effect of different tartaric stabilization options on the color and phenolic composition of Tannat red wines from Uruguay.

AGEING BOTTLED WINES SUBMERGED IN SEA: DOES IT IMPACT WINE COMPOSITION?

Aging wines is a common practice in oenology, which in recent years has undergone some innovations. Currently, we are witnessing the practice of aging bottled wine in depth, immersed in the sea or in reservoirs, for variable periods of time, but so far, little is known about the impact of aging in depth on the physicochemical properties, of wines.
The objective of this work was to evaluate the impact of this practice on the physicochemical characteristics, in particular to verify changes in the volatile composition of wines bottled and subsequently immersed in depth. A red wine from Cabernet Sauvignon was bottled and a set of bottles were submerged from July to February (2020), another set of bottles were submerged from February to September (2020) and another set was kept in the wine cellar. Bottles from each set were analyzed (in triplicate) in July 2021.