terclim by ICS banner
IVES 9 IVES Conference Series 9 EFFECTS OF LEAF REMOVAL AT DIFFERENT BUNCHES PHENOLOGICAL STAGES ON FREE AND GLYCOCONJUGATE AROMAS OF SKINS AND PULPS OF TWO ITALIAN RED GRAPES

EFFECTS OF LEAF REMOVAL AT DIFFERENT BUNCHES PHENOLOGICAL STAGES ON FREE AND GLYCOCONJUGATE AROMAS OF SKINS AND PULPS OF TWO ITALIAN RED GRAPES

Abstract

Canopy-management practices are applied in viticulture to improve berries composition and quality, having a great impact on primary and secondary grape metabolism. Among these techniques, cluster zone leaf removal (defoliation) is widely used to manage air circulation, temperature and light radiation of grape bunches and close environment. Since volatiles are quantitatively and qualitatively influenced by the degree of fruit ripeness, the level of solar exposure, and the thermal environment in which grapes ripen, leaf removal has been shown to affect volatile composition of grape berries [1].

The aim of this research was to assess the impact of vine defoliation on free and glycosylated VOCs (Volatile Organic Compounds) of two Italian red grapes: Nebbiolo (neutral) and Aleatico (semi-aromatic). Defoliation was performed at fruit set phenological stage for Aleatico grapevines, and for Nebbiolo also at berries touch.

Solid Phase Extraction/Gas Chromatography–Mass Spectrometry (SPE/GC-MS) was carried out to ana-lyse free and glycoconjugates VOCs isolated from skins and pulps as separate portions of the berries [2].

The results showed that defoliation had an almost negligible effect on free and glycosylated volatiles of Aleatico grapes, thus suggesting that defoliation at fruit set did not change the volatile composition of this grape variety. A different behaviour was observed for Nebbiolo grapes, on both free and bound VOCs, with a greater impact on the first. Indeed, all the 30 free VOCs identified were significantly (ANOVA; p<0.05) affected by defoliation and by the time at which it was carried out, with a greater influence on the skin components. Early defoliation at fruit set did not favour the accumulation of free VOCs in Nebbiolo skins, significantly reducing the content of several VOCs, such as n-butyl acetate, terpenes (α-terpineol, and nerol) and aldehydes (hexanal, and 2-hexanal). On the other hand, late defoliation performed at berries touch, reduced n-butyl acetate, but increased alcohols content (i.e., 3-methyl-1-butanol, 1-pentanol, 2-ethyl-1-hexanol, benzyl and phenylethyl alcohols), nerol, aldehydes (i.e., 2-hexanal), and vanillin.

Results suggest that the effects of defoliation at fruit set on the VOCs pattern is cultivar dependent and almost ineffective on Aleatico grapes. Moreover, early defoliation at fruit-set seems stressful for Neb-biolo grapes and its odorous and potentially secondary metabolites. These results can be useful to improve canopy and winemaking precision practices.

 

1. Poni et al., 2006. DOI: 10.5344/ajev.2006.57.4.397
2. Piombino et al., 2022. DOI: 10.1111/ajgw.12521

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Paola Piombino¹, Elisabetta Pittari¹, Alessandro Genovese², Andrea Bellincontro³, Osvaldo Failla⁴, Luigi Moio1, Fabio Mencarelli⁵

1. Department of Agricultural Sciences, Division of Vine and Wine Sciences, University of Naples Federico II, Avellino 83100, Italy
2. Department of Agricultural Sciences, Division of Food Science and Technology, University of Naples Federico II, Portici (NA), 80055, Italy
3. DIBAF, University of Tuscia, Via De Lellis, 01100 Viterbo, Italy
4. Department of Agricultural and Environmental Sciences, University of Milan, 20133 Milano, Italy
5. Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto 80, Pisa 56124, Italy

Contact the author*

Keywords

defoliation, secondary metabolites, aromas

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

FACTORS AFFECTING QUERCETIN SOLUBILITY IN SANGIOVESE RED WINE: FIRST RESULTS

Quercetin (Q) is present in grape in form of glycosides and as aglycone. These compounds are extracted from grape skins during winemaking. In wines, following the hydrolysis reactions, the amount of quercetin aglycon can exceed its solubility value. Unfortunately, a threshold solubility concentration for quercetin in wine is not easy to determine because it depends on wine matrix (Gambuti et al., 2020).
Read More

USE OF 13C CP/MAS NMR AND EPR SPECTROSCOPIC TECHNIQUES TO CHARACTERIZE MACROMOLECULAR CHANGES IN OAK WOOD(QUERCUS PETRAEA) DURING TOASTING

For coopers, toasting process is considered a crucial step in barrel production during which oak wood (Q. petraea) develops several aromatic nuances released to the wine during its maturation. Toasting consists of applying different degrees of heat to a barrel for a specific period. As the temperature increases, thermal degradation of oak wood structure produces a huge range of chemical compounds. Many studies have identified the main key aroma volatile compounds (whisky-lactone, furfural, eugenol, guaiacol, vanillin). However, detailed information on how the chemical structure of oak wood degrades with increasing toasting level is still lacking.

Read More

PAIRING WINE AND STOPPER: AN OLD ISSUE WITH NEW ACHIEVEMENTS

The sensory characteristics of wine are a topic studied by several researchers over time, but it continues to be a current and challenging subject. These characteristics are fundamental for the consumer acceptability, which has increasingly aroused their interest to modulate them in line with current market trends and innovation demands. The wine physical-chemical and sensory properties depend on a wide set of factors: they begin to be designed in the vineyard and are later constructed during the various stages of winemaking. Afterwards, the wine is placed in bottles and stored or commercialized.

Read More

NEW INSIGHTS INTO THE EFFECT OF TORULASPORA DELBRUECKII/SACCHAROMYCES CEREVISIAE INOCULATION STRATEGY ON MALOLACTIC FERMENTATION PERFORMANCE

Winemaking is influenced by micro-organisms, which are largely responsible for the quality of the product. In this context, Non-Saccharomyces and Saccharomyces species are of great importance not only because it influences the development of alcoholic fermentation (AF) but also on the achievement of malolactic fermentation (MLF). Among these yeasts, Torulaspora delbrueckii allows in sequential inoculation with strains of S. cerevisiae shorter MLF realizations [5] . Little information is available on the temporal effect of the presence of T. delbrueckii on (i) the evolution of AF and (ii) the MLF performance.

Read More

INVESTIGATION INTO MOUSY OFF-FLAVOR IN WINE USING GAS CHROMATOGRAPHY-MASS SPECTROMETRY WITH STIR BAR SORPTIVE EXTRACTION

Mousy off-flavor is one of the defects of microbial origin in wine. It is described as a particularly unpleasant defect reminiscent of rodent urine (a “dirty mouse cage”), and grilled foods such as popcorn, rice, crackers, and bread crust. Prior to the 2010s, mousiness was very uncommon but it has been becoming more frequent in recent years. It is often associated with an increase in pH as well as certain oenological practices, which tend to significantly decrease the use of sulfur dioxide.

Read More