terclim by ICS banner
IVES 9 IVES Conference Series 9 MONOSACCHARIDE COMPOSITION AND POLYSACCHARIDE FAMILIES OF LYOPHILISED EXTRACTS OBTAINED FROM POMACES OF DIFFERENT WHITE GRAPE VARIETIES

MONOSACCHARIDE COMPOSITION AND POLYSACCHARIDE FAMILIES OF LYOPHILISED EXTRACTS OBTAINED FROM POMACES OF DIFFERENT WHITE GRAPE VARIETIES

Abstract

The recovery of bioactive compounds from grape and wine by-products is currently an important and necessary objective for sustainability. Grape pomace is one of the main by-products and is a rich source of some bioactive compounds such as polyphenols, polysaccharides, fatty acids, minerals and seed oil. Polysaccharides contained in the grape cell wall can be rhamnogalacturonans type II (RG-II), polysaccharides rich in arabinose and galactose (PRAG), mannoproteins (MP), homogalacturonans (HG) and non pectic polysaccharides (NPP). The aim of this study was to evaluate the monosaccharide composition and polysaccharide families of extracts obtained from pomaces of different white grape varieties.

Twelve grape pomaces from 9 different white grape varieties of Castilla y León region were analysed after destemming and pressing the grapes. The polysaccharide extraction was carried out with the method previously developed by Canalejo et al. (2021). The lyophilised extracts were analysed by gas chromatography with mass spectrometry detector following the conditions developed by Guadalupe et al. (2012). An ANOVA and a principal component analysis (PCA) were carried out to determine the differences between grape varieties using the RStudio program.

Statistically significant differences were found between the white grape pomaces studied and even within the same grape variety. The Verdejo and Puesta en Cruz varieties showed the highest polysaccharide content due to the highest content in glucose and galacturonic acid. In addition, the Puesta en Cruz variety stood out for its higher rhamnose and galactose content. On the other hand, the Viura grape variety with highest maturity degree and the Sauvignon Rytos presented the lowest polysaccharide concentration. Considering the percentage of the different polysaccharide families, the PCA selected two components with an eigenvalue greater than 1, which explained 92.3% of the total variance. The Verdejo, Viura, Rufete Serrano and Soreli were more associated to the percentage of NPP, while the Malvasía, Puesta en Cruz and Sauvignon Blanc were more correlated with the HG and PRAGs.

ACKNOWLEDGEMENTS: The authors would like to thank the Agencia Estatal de Investigación (AEI) and the Ministerio de Ciencia e Innovación (MICINN) for the funding provided for this study through the project PID2021-123361OR-C21 (with FEADER funds). M. C-F. also thanks the MICINN and AEI for funding her predoctoral contract (PRE2020-094464, with FSE funds).

 

1. Canalejo, D.; Guadalupe, Z.; Martínez-Lapuente, L.; Ayestarán, B.; Pérez-Magariño, S. Optimization of a method to extract polysaccharides from white grape pomace by-products. Food Chem. 2021, 365, 130445.
2. Guadalupe, Z.; Martínez-Pinilla, O.; Garrido, Á.; Carrillo, J.; Ayestarán, B. Quantitative determination of wine polysaccharides by gas chromatography-mass spectrometry (GC-MS) and size exclusion chromatography (SEC). Food Chem. 2012, 131, 367–374.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

María Curiel-Fernández¹, Belén Ayestarán², Zenaida Guadalupe², Silvia Pérez-Magariño¹

1. Instituto Tecnológico Agrario de Castilla y León, Ctra Burgos Km 119, 47071 Valladolid, Spain.
2. Instituto de Ciencias de la Vid y el Vino (Universidad de La Rioja, Gobierno de La Rioja, CSIC), Finca de La Grajera, Ctra. Burgos 6, 26007 Logroño, Spain.

Contact the author*

Keywords

white pomace, monosaccharides, grape polysaccharides, by-products

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

INVESTIGATION OF FILM COATINGS AS A PROTECTIVE LAYER IN REDUCING THE ABSORPTION OF SMOKE PHENOLS INTO PINOT NOIR GRAPES

Wine grapes exposed to wildfire smoke have resulted in wines with burnt and ashy sensory characteristics¹, that are undesirable qualities in wine. In extreme wildfire events, this can lead to total loss of grape crop. Currently there are no effective solutions in the market to prevent the uptake of smoke compounds into grapes. In this study, previously developed innovative film coatings were tested to analyze their effectiveness in reducing smoke phenol absorption². Four different cellulose nanofiber-based film types were investigated.

Searching for the sweet spot: a focus on wine dealcoholization

It is well known that the vinification of grapes at full maturation can produce rich, full-bodied wines,
with intense and complex flavour profiles. However, the juice obtained from such grapes may have very
high sugar concentration, resulting in wines with an excessive concentration of ethanol. In addition, the decoupling between technological maturity and phenolic/aromatic one due to global warming, exacerbates this problem in some wine-growing regions. In parallel with the increase of the mean alcohol content of wines on the market, also the demand for reduced alcohol beverages has increased in recent years, mainly as a result of health and social concerns about the risks related to the consumption of alcohol.

AN AUTOMATIC CANOPY COOLING SYSTEM TO COPE WITH THE THERMAL-RADIATIVE STRESSES IN THE PIGNOLETTO WHITE GRAPE

In recent years characterized by hot dry summers, the implementation of innovative irrigation tools in the vineyard represents a crucial challenge to ensure optimal production and to avoid excess of water consumption. It is known that the grapevine reacts to multiple stresses – i.e., high temperatures and wa- ter shortage – through adaptive mechanisms that are detrimental to the yield. Furthermore, this condi- tion is usually aggravated by high solar radiation, which could negatively affect the phenolic composi- tion of the grapes. Therefore, a cooling system has been developed aiming to reduce bunches’ sunburn damage.

HOW OXYGEN CONSUMPTION INFLUENCES RED WINES VOLTAMMETRIC PROFILE

Phenolic compounds play a central role in sensory characteristics of wine, such as colour, mouthfeel, flavour and determine its shelf life. Furthermore, the major non-enzymatic wine oxidation process is due to the catalytic oxidation of phenols in quinones. Due their importance, during the years have been developed different analytical methods to monitor the concentration of phenols in wine, such as Folin-Ciocalteu method, spectrophotometric techniques and HPLC. These methods can also be used to follow some oxidation-related chemical transformations.

SENSORY CHARACTERIZATION OF COGNAC EAUX-DE-VIE AGED IN BARRELS REPRESENTING DIFFERENT TOASTING PROCESS

Cognac is an outstanding french wine spirit appreciated around the world and produced exclusively in the Nouvelle-Aquitaine region, and more precisely in the Cognac area. According to AOC regulations (Appellation D’origine Controlée), the spirit required at least 2 years of continuous ageing in oak barrels to be granted the title of Cognac. The oak wood will import color, structure and organoleptic complexity. The different steps during barrel-making process, such as seasoning and toasting, influence the above quality attributes in both wines and spirits.