terclim by ICS banner
IVES 9 IVES Conference Series 9 MONOSACCHARIDE COMPOSITION AND POLYSACCHARIDE FAMILIES OF LYOPHILISED EXTRACTS OBTAINED FROM POMACES OF DIFFERENT WHITE GRAPE VARIETIES

MONOSACCHARIDE COMPOSITION AND POLYSACCHARIDE FAMILIES OF LYOPHILISED EXTRACTS OBTAINED FROM POMACES OF DIFFERENT WHITE GRAPE VARIETIES

Abstract

The recovery of bioactive compounds from grape and wine by-products is currently an important and necessary objective for sustainability. Grape pomace is one of the main by-products and is a rich source of some bioactive compounds such as polyphenols, polysaccharides, fatty acids, minerals and seed oil. Polysaccharides contained in the grape cell wall can be rhamnogalacturonans type II (RG-II), polysaccharides rich in arabinose and galactose (PRAG), mannoproteins (MP), homogalacturonans (HG) and non pectic polysaccharides (NPP). The aim of this study was to evaluate the monosaccharide composition and polysaccharide families of extracts obtained from pomaces of different white grape varieties.

Twelve grape pomaces from 9 different white grape varieties of Castilla y León region were analysed after destemming and pressing the grapes. The polysaccharide extraction was carried out with the method previously developed by Canalejo et al. (2021). The lyophilised extracts were analysed by gas chromatography with mass spectrometry detector following the conditions developed by Guadalupe et al. (2012). An ANOVA and a principal component analysis (PCA) were carried out to determine the differences between grape varieties using the RStudio program.

Statistically significant differences were found between the white grape pomaces studied and even within the same grape variety. The Verdejo and Puesta en Cruz varieties showed the highest polysaccharide content due to the highest content in glucose and galacturonic acid. In addition, the Puesta en Cruz variety stood out for its higher rhamnose and galactose content. On the other hand, the Viura grape variety with highest maturity degree and the Sauvignon Rytos presented the lowest polysaccharide concentration. Considering the percentage of the different polysaccharide families, the PCA selected two components with an eigenvalue greater than 1, which explained 92.3% of the total variance. The Verdejo, Viura, Rufete Serrano and Soreli were more associated to the percentage of NPP, while the Malvasía, Puesta en Cruz and Sauvignon Blanc were more correlated with the HG and PRAGs.

ACKNOWLEDGEMENTS: The authors would like to thank the Agencia Estatal de Investigación (AEI) and the Ministerio de Ciencia e Innovación (MICINN) for the funding provided for this study through the project PID2021-123361OR-C21 (with FEADER funds). M. C-F. also thanks the MICINN and AEI for funding her predoctoral contract (PRE2020-094464, with FSE funds).

 

1. Canalejo, D.; Guadalupe, Z.; Martínez-Lapuente, L.; Ayestarán, B.; Pérez-Magariño, S. Optimization of a method to extract polysaccharides from white grape pomace by-products. Food Chem. 2021, 365, 130445.
2. Guadalupe, Z.; Martínez-Pinilla, O.; Garrido, Á.; Carrillo, J.; Ayestarán, B. Quantitative determination of wine polysaccharides by gas chromatography-mass spectrometry (GC-MS) and size exclusion chromatography (SEC). Food Chem. 2012, 131, 367–374.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

María Curiel-Fernández¹, Belén Ayestarán², Zenaida Guadalupe², Silvia Pérez-Magariño¹

1. Instituto Tecnológico Agrario de Castilla y León, Ctra Burgos Km 119, 47071 Valladolid, Spain.
2. Instituto de Ciencias de la Vid y el Vino (Universidad de La Rioja, Gobierno de La Rioja, CSIC), Finca de La Grajera, Ctra. Burgos 6, 26007 Logroño, Spain.

Contact the author*

Keywords

white pomace, monosaccharides, grape polysaccharides, by-products

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

HOW DO ROOTSTOCKS AFFECT CABERNET SAUVIGNON AROMATIC EXPRESSION?

Grape quality potential for wine production is strongly influenced by environmental parameters such as climate and agronomic factors such as rootstock. Several studies underline the effect of rootstock on vegetative growth of the scions [1] and on berry composition [2, 3] with an impact on wine quality. Rootstocks are promising agronomic tools for climate change adaptation and in most grape-growing regions the potential diversity of rootstocks is not fully used and only a few genotypes are planted. Little is known about the effect of rootstock genetic variability on the aromatic composition in wines; thus further investigations are needed.

FOURIER TRANSFORM INFRARED SPECTROSCOPY IN MONITORING THE WINE PRODUCTION

The complexity of the wine matrix makes the monitoring of the winemaking process crucial. Fourier Transform Infrared Spectroscopy (FTIR) along with chemometrics is considered an effective analytical tool combining good accuracy, robustness, high sample throughput, and “green character”. Portable and non-portable FTIR devices are already used by the wine industry for routine analysis. However, the analytical calibrations need to be enriched, and some others are still waiting to be thoroughly developed.

BIOPROTECTION BY ADDING NON-SACCHAROMYCES YEASTS : ADVANCED RESEARCH ON THIS PROMISING ALTERNATIVE TO SO₂

Sulphur dioxide has been used for many years for its antimicrobial, antioxidant and antioxydasic properties in winemaking but nowadays, it is a source of controversy. Indeed, consumers are more attentive to the naturalness of their foods and beverages and the legislation is changing to reduce the total SO₂ levels allowed in wines. To limit and replace the doses of sulphur dioxide applied, winemakers can now use bioprotection consisting in live yeast addition as alternative,seems to be promising. This process, lightly used in from the food industry, allows to colonize the environment and limit the development or even eliminate undesirable microorganisms without altering the sensory properties of the product.

YEAST DERIVATIVE PRODUCTS: CHARACTERIZATION AND IMPACT ON RIBOFLAVIN RELEASE DURING THE ALCOHOLIC FERMENTATION

Light-struck taste (LST) is a wine fault that can occur in white and sparkling wines when exposed to light. This defect is mainly associated to the formation of methanethiol and dimethyl disulfide due to light-induced reactions involving riboflavin (RF) and methionine [1]. The presence of RF in wine is mainly due to the metabolism of yeast [2] which fermenting activity can be favoured by using yeast derivative products (YDPs) as nutrients. Nonetheless, a previous study showed the addition of YDPs before the alcoholic fermentation (AF) led to higher concentrations of RF in wines [3]. Due to the widespread use of YDPs in the winemaking process, this study aimed to understand the possible relation between the content of RF in wine and the YDP adopted as nutrient for AF.

FUNGAL DIVERSITY AND DYNAMICS IN CHAMPAGNE VINEYARDS: FROM VINE TO WINE

Champagne is a well-known wine region in Northern France with distinct terroirs and three main grape varieties. As for any vineyard, wine quality is highly linked to the microbiological characteristics of the raw materials. However, Champagne grape microbiota, especially its fungal component, has yet to be fully characterized. Our study focused on describing this mycobiota, from vine to small scale model wine, for the two main Champagne grape varieties, Pinot Noir and Meunier, using complementary cultural and omics approaches.