GiESCO 2019 banner
IVES 9 IVES Conference Series 9 GiESCO 9 GiESCO 2019 9 Towards the definition of a detailed transcriptomic map of grape berry development

Towards the definition of a detailed transcriptomic map of grape berry development

Abstract

Context and purpose of the study ‐ In the last years the application of genomic tools to the analysis of gene expression during grape berry development generated a huge amount of transcriptomic data from different varieties and growing conditions. This information set the stage to understand the molecular basis of crucial developmental and metabolic rearrangements occurring during grape berry formation and ripening. It is now clear that the variation of a portion of berry transcriptome is conserved across cultivars and growing conditions, and thus may be used universally to describe the stage of berry development. In this work we explore the possibility of using the transcriptomic data generated from two cultivars to define a very detailed developmental map of the grape berry.

Material and methods ‐ To map the molecular events associated with berry development at very high temporal resolution, we performed RNA‐seq analysis of berry samples collected every week from fruit‐ set to maturity from Pinot noir and Cabernet Sauvignon vines grown in the same location. The experiment was replicated across three consecutive years (2012, 2013, 2014) resulting in 219 samples overall. Applying multivariate analyses to the most variable portion of the transcriptome, we built a transcriptomic model of berry development based on the molecular information obtained from samples of both cultivars.

Results ‐ The Pinot noir and Cabernet Sauvignon samples mostly aligned in a 3D transcriptomic map (~80% of the variance described by Principal Component Analysis), allowing to define a general model of berry development based on gene expression. The performance of the model in describing the development of other grape varieties was accessed projecting RNA‐seq samples of fruit development of ten Italian cultivars onto the model. Both red and white‐skin berry samples mapped on the transcriptomic map and revealed alignment by standard ripening parameters (e.g. total soluble solids) as well as unrelated to any of these. Moreover, we validated that berry maturation of the same cultivar cultivated in different International growing regions can be well represented and aligned by means of our transcriptomic map. These results showed that the transcriptomic information can be accessed to precisely define a model of “molecular phenology” that can be used to map the ontogenetic development of the fruit with high precision and to align the stage of berry development of different grapes. 

DOI:

Publication date: June 19, 2020

Issue: GiESCO 2019

Type: Article

Authors

Marianna FASOLI (1), Chandra L. RICHTER (1), Sara ZENONI (2), Marco SANDRI (2), Paola ZUCCOLOTTO (3), Mario PEZZOTTI (2), Nick DOKOOZLIAN (1), and Giovanni Battista TORNIELLI(2)

(1) E&J Gallo Winery, Modesto, CA 95353, USA
(2) Department of Biotechnology, University of Verona, 37134 Verona, Italy
(3) Big & Open Data Innovation Laboratory, University of Brescia, 25123 Brescia, Italy

Contact the author

Keywords

Grapevine, Berry development, Ripening, Molecular Phenology, Transcriptomics

Tags

GiESCO 2019 | IVES Conference Series

Citation

Related articles…

The effects of cover cropping systems on vine physiology, berry and wine quality in a climate change scenario in Switzerland

Sustainable weed control with little detrimental effects on vine physiology, yield, berry quality, soil structure, health and biodiversity is a key factor in vineyard management. Few options are available to avoid herbicide utilization and minimize negative effects of frequent tillage on soil quality. The present project aims to investigate and develop different cover management strategies in a cool climate viticultural region in Switzerland. The impact of different treatments on vine, must and wine has been studied in an experimental vineyard in Changins, Switzerland for one year and will be continued over the next three years.

EFFECT OF FUMARIC ACID ON SPONTANEOUS FERMENTATION IN GRAPE MUST

Malolactic fermentation (MLF)¹, the decarboxylation of L-malic acid into L-lactic acid, is performed by lactic acid bacteria (LAB). MLF has a deacidifying effect that may compromise freshness or microbiological stability in wines² and can be inhibited by fumaric acid [E297] (FA). In wine, can be added at a maximum allowable dose of 0.6 g/L³. Its inhibition with FA is being studied as an alternative strategy to minimize added doses of SO₂⁴. In addition, wine yeasts are capable of metabolizing and storing small amounts of FA and during alcoholic fermentation (AF).

Identification of a stable epi-allele associated with flower development and low bunch compactness in a somatic variant of Tempranillo Tinto

Grapevine cultivars are vegetatively propagated to preserve their varietal characteristics. However, spontaneous somatic variations that occur and are maintained during cycles of vegetative growth offer opportunities for the natural improvement of traditional grape cultivars. One advantageous trait for winegrowing is reduced bunch compactness, which decreases the susceptibility to pests and fungal diseases and favor an even berry ripening.

Correlation between grape and wine quality, landscape diversity, on-field biodiversity, in doc gioia del colle, italy

Analysis of aerial photos by using GIS tools and on-field surveys of flora are used to characterize territories from an agro-ecological point of view and to assess the level of diversity of given agro-ecosystems. More and more correlations between landscape characteristics, sustainability and quality of agriculture production were speculated. In last three years a study was carried out in the area of DOC “Gioia del Colle” in Apulia, South Italy, in order to characterize and investigate different vineyards and sites and find out possible interactions and correlations between the landscape diversity, the biodiversity of fields and the quality of grapes and wines.

Aroma characterisation of mold resistant sparkling wines produced in a warm-temperate area

In recent years, resistant varieties have returned to the attention of the wine sector as a response to climate change and the reduction of pesticides in grapevine management, which is the main culprit of pesticide use in European agriculture. In this context, the production of sparkling wines could be strongly influenced due to its requirements for a particular balance between sugars and acidity, and the necessity of sound grapes to ensure wine quality. However, these parameters are not the only ones that define the suitability of a grape variety to produce sparkling wine.