Macrowine 2021
IVES 9 IVES Conference Series 9 Extraction of polyphenols from grape marc by supercritical fluid extraction (SFE) and evaluation of their ‘bioavailability’ as dietary supplements

Extraction of polyphenols from grape marc by supercritical fluid extraction (SFE) and evaluation of their ‘bioavailability’ as dietary supplements

Abstract

In the winemaking process, several compounds that remain in the grape skins and seeds after the fermentation stage are bioactive-compounds (substances with potential beneficial effects on health) that can be extracted in order to recovery valuable substances with a high commercial value for the cosmetic, food (nutraceuticals) and pharmaceutical industries. The skins contain significant amounts of bioactive substances such as tannins (16-27%) and other polyphenolic compounds (2-6.5%) in particular, catechins, anthocyanins, proanthocyanins, quercetin , ellagic acid and resveratrol. The seeds, in addition to oil, contain approximately 60% of the polyphenols present in grape, in particular an high concentration of flavan-3-ols, catechin and epicatechin. Grape seed extracts are very potent antioxidants and exhibit numerous interesting pharmacologic activities The traditional extraction methods used for polyphenols from solid or semi-solid materials have been focused on methods, which use organic solvents. These methods are laborious and time consuming, promote degradation reactions, have low selectivity and/or low extraction yields. Moreover, these conventional techniques employ large amounts of toxic solvents. Some of these critical points could be over boost with Supercritical Fluid Extraction (SFE). The most used solvent in extraction with supercritical fluids is the CO2, which is economical, safe, non-toxic (it does not leave residues in extract) and reaches supercritical conditions easily (32°C and 74 bar). It is suitable for thermo-labile substances being the temperature of its critical point 32°C. In the supercritical phase it is selective towards apolar compounds or weakly polar, so it is necessary to add co-solvents (ethanol and water are the co-solvents used in food processing) in order to extract the polar compounds. Applying this technology the thermal and chemical degradation of the products, which are completely free from processing residues, is prevented, while the solvent power and selectivity can be easily adjusted from gas-like to liquid-like by changing the pressure and temperature of the extraction, making thus possible the fractionation of the extract. SFE is a Green Technology and this guarantees competitive advantage in conjunction with sustainable development. The extraction of phenolic compounds from grape marc using supercritical CO2 containing 15% ethanol–water mixture (57%, v/v) (EtW) as co-solvent, at 8, 10, 20 and 30 MPa/313.15 K suggested 8 MPa as the most suitable pressure. The evaluation of the ‘bioavailability’ of the grape-CO2 extracts so obtained was carried out . The ‘bioavailability’ is a key step in ensuring ‘bioefficacy’ of bioactive compounds when used as supplements because they need to be bioavailable in order to exert any beneficial effects on human health.

Publication date: April 4, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Carla Da Porto*, Andrea Natolino, Dario Vojnovic, Deborha Decorti

*University of Udine

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Intelligent article to control the internal pressure in continue in bottles

An intelligent packaging might, among others, provide information and allow monitoring of the quality of the packed product or its surrounding environment. A recent project on micro-flow wine bottles closed with aluminium screw cap and tightness liner, highlighted the importance of monitoring the internal overpressure continuously, in real-time and at least for 72 hours, since the internal pressure on the tightness liner and the micro-flow are related. Real-time and continuous measurements are not the standard methods of measurement of the overpressure, yet. The most used equipment for the determination of the pressure in wine bottle is the aphrometer, a destructive device that supplies a single value of pressure.

Evaluating South African Chenin blanc wine styles using an LC-MS screening method

Sorting Chenin blanc is one of the most important white wine cultivars in South Africa. It has received a lot of attention and accolades in the past years and more research than ever is dedicated to this versatile cultivar. According to the Chenin blanc association of South Africa, there are three recognized dry wine styles, Fresh and Fruity (FF), Rich and Ripe Unwooded
(RRU), and Rich and Ripe Wooded (RRW). They are traditionally established with the aid of expert sensory evaluation, but the cost and the (subjective) human factor are aspects to be taken into account. A more objective and possibly robust way of assessing and attributing these styles can be the use of chemical analysis.

Full automation of oenological fermentations and its application to the processing of must containing high sugar or acetic acid concentrations

Climate change and harvest date decisions have led to the evolution of must quality over the last decades. Increases in must sugar concentrations are among the most obvious consequences, quantitatively. Saccharomyces cerevisiae is a robust and acid tolerant organism. These properties, its sugar to ethanol conversion rate and ethanol tolerance make it the ideal production organism for wine fermentations. Unfortunately, high sugar concentrations may affect S. cerevisiae and lead to growth inhibition or yeast lysis, and cause sluggish or stuck fermentations. Even sublethal conditions cause a hyperosmotic stress response in S. cerevisiae which leads to increased formation of fermentation by-products, including acetic acid, which may exceed legal limits in some wines.

Novel contribution to the study of mouth-feel properties in wines

In general, there is a well-established lexicon related to wine aroma and taste properties; however mouth-feel-related vocabulary usually includes heterogeneous, multimodal and personalized terms. Gawel et al.
(2000) published a wheel related to mouthfeel properties of red wine. However, its use in scientific publications has been limited. The authors accepted that the approach had certain limitations as it included redundant and terms with hedonic tone and some others were absent. It is of high interest to generate a mouth-feel lexicon and finding the chemical compound or group of compounds responsible for such properties in red wine. In the present work a chemical fractionation method has been developed.

Foam characteristics of white, rosé and red sparkling wines elaborated by the champenoise method

Contribution Foam is the characteristic that differentiates sparkling wines from still wines, being the first sensory attribute that tasters and consumers perceive and that determines the final quality of sparkling wines [1]. The foaming properties mainly depend on the chemical composition of wines [2-3], and different factors involved in wine composition will have an effect on foam quality. In Spain, the sparkling wine market focuses on the production of white and rosé sparkling wine, with very low production of red sparkling wines. However, this type of wines is elaborated in countries like Australia, South-Africa, Argentina, Italy or Portugal, with a great acceptance by consumers. No studies on the foaming characteristics of red sparkling wines have been found.