Macrowine 2021
IVES 9 IVES Conference Series 9 Prediction of the production kinetics of the main fermentative aromas in alcoholic fermentation

Prediction of the production kinetics of the main fermentative aromas in alcoholic fermentation

Abstract

Fermentative aromas (especially esters and higher alcohols) highly impact the organoleptic profile of young and white wines. The production of these volatile compounds depends mainly on temperature and Yeast Available Nitrogen (YAN) content in the must. Available dynamic models predict the main reaction (bioconversion of sugar into ethanol and CO2 production) but none of them considers the production kinetics of fermentative aroma compounds during the process of fermentation. We determined the production kinetics of the main esters and higher alcohols for different values of initial YAN content and temperature, using an innovative online monitoring Gas Chromatography device. We then elaborated a dynamic model predicting the synthesis of five fermentative aromas representative of three different chemical families: two higher alcohols (isobutanol, isoamyl alcohol), one acetate ester (isoamyl acetate) and two ethyl esters (ethyl hexanoate, ethyl octanoate). The online monitoring highlighted two successive linear phases of aroma compound production from sugar. We therefore began by modeling changes in the production yields of these compounds (aroma compound vs. sugar) depending on initial nitrogen concentration and temperature. We then integrated these yields into a previously developed model of the kinetics of sugar consumption during the fermentation process. We thus obtained a dynamic model predicting the production kinetics of volatile compounds throughout the alcoholic fermentation from initial nitrogen concentration and temperature values. The parameters of the model were identified from nine fermentations performed at temperatures between 18 and 30 ◦C and with initial YAN contents ranging from 70 to 410 mgN/L. The model was validated in six independent experiments with conditions in the same range. Predictions were accurate: the mean difference between experimental and estimated values for fermentative aroma synthesis throughout the process was below 10%, for both the fermentations used to build the model and those used for validation. This model is the first to simulate the production kinetics of fermentative aromas and provides new insight into the synthesis of these volatile compounds. It will facilitate the development of innovative strategies for controlling the production of those aromas in winemaking, through management of the principal control factors: YAN content and temperature during the alcoholic fermentation.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Jean-Roch Mouret*, Cristian Trelea, Jean-Marie Sablayrolles, Vincent Farines

*INRA

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Microbial stabilization of wines using innovative coiled UV-C reactor process: impact on chemical and organoleptic proprieties

For several years, numerous studies aimed at limiting the use of SO2 in wines (thermal treatments, pulsed electric fields, microwaves …). Processes must be able to preserve the organoleptic qualities of wines with low energy consumption. In this context, ultraviolet radiations (UV-C), at 254 nm, are well known for their germicidal proprieties. In order to inactivate microorganisms in grape juice and wine without affecting the quality of the product, efficiency of UV-C treatment process should be optimized.

New biological tools to control and secure malolactic fermentation in high pH wines

Originally, the role of the malolactic fermentation (MLF) was simply to improve the microbial stability of wine via biological deacidification. However, there is an accumulation of evidence to support the fact that lactic acid bacteria (LAB) also contribute positively to the taste and aroma of wine. Many different LAB enter into grape juice and wine from the surface of grape berries, cluster stems, vine leaves, soil and winery equipment. Due to the highly selective environment of juices and wine, only a few types of LAB are able to grow.

Elicitors used as a tool to increase stilbenes in grapes and wines

The economic importance of grapevine as a crop plant makes Vitis vinífera a good model system to study the improvement of the nutraceutical properties of food products (Vezulli et al. 2007). Stilbenes in general, and trans-resveratrol in particular, have been reported to be responsible for various beneficial effects. Resveratrol´s biological properties include antibacteria and antifungal effects, as well as cardioprotective, neuroprotective and anticâncer actions (Guerrero et al. 2010 ). Stilbenes can be induced by biotic and abiotic elicitors since they are phytoalexins (Bavaresco et al. 2001).

Monitoring of Pesticide Residues from Vine to Wine

Those previous years, pesticides are often brought to the forefront by media. Questions arose about their toxicity for growers and consumers. Even if a downward trend is underway, the use of pesticides is required to ensure steady quality and quantity of harvests. A large number of active ingredients are authorized but regarding viticulture, mainly insecticides and fungicides are applied, to control pests and diseases and to increase crop yield. Some phytosanitary products, principally fungicides, applied close to the harvest date may frequently be detected in wines.

Anti/prooxidant activity of wine polyphenols in reactions of adrenaline auto-oxidation

Adrenaline (epinephrine) belongs to catecholamine class. It is a neurotransmitter and both a hormone which is released by the sympathetic nervous system and adrenal medulla in response to a range of stresses in order to regulate blood pressure, cardiac stimulation, relaxation of smooth muscles and other physiological processes. Adrenaline exhibits an effective antioxidant capacity (1). However, adrenalin is capable to auto-oxidation and in this case it generates toxic reactive oxygen intermediates and adrenochrome. Under in vitro conditions, auto-oxidation of adrenaline occurs in an alkaline medium (2).