Macrowine 2021
IVES 9 IVES Conference Series 9 Prediction of the production kinetics of the main fermentative aromas in alcoholic fermentation

Prediction of the production kinetics of the main fermentative aromas in alcoholic fermentation

Abstract

Fermentative aromas (especially esters and higher alcohols) highly impact the organoleptic profile of young and white wines. The production of these volatile compounds depends mainly on temperature and Yeast Available Nitrogen (YAN) content in the must. Available dynamic models predict the main reaction (bioconversion of sugar into ethanol and CO2 production) but none of them considers the production kinetics of fermentative aroma compounds during the process of fermentation. We determined the production kinetics of the main esters and higher alcohols for different values of initial YAN content and temperature, using an innovative online monitoring Gas Chromatography device. We then elaborated a dynamic model predicting the synthesis of five fermentative aromas representative of three different chemical families: two higher alcohols (isobutanol, isoamyl alcohol), one acetate ester (isoamyl acetate) and two ethyl esters (ethyl hexanoate, ethyl octanoate). The online monitoring highlighted two successive linear phases of aroma compound production from sugar. We therefore began by modeling changes in the production yields of these compounds (aroma compound vs. sugar) depending on initial nitrogen concentration and temperature. We then integrated these yields into a previously developed model of the kinetics of sugar consumption during the fermentation process. We thus obtained a dynamic model predicting the production kinetics of volatile compounds throughout the alcoholic fermentation from initial nitrogen concentration and temperature values. The parameters of the model were identified from nine fermentations performed at temperatures between 18 and 30 ◦C and with initial YAN contents ranging from 70 to 410 mgN/L. The model was validated in six independent experiments with conditions in the same range. Predictions were accurate: the mean difference between experimental and estimated values for fermentative aroma synthesis throughout the process was below 10%, for both the fermentations used to build the model and those used for validation. This model is the first to simulate the production kinetics of fermentative aromas and provides new insight into the synthesis of these volatile compounds. It will facilitate the development of innovative strategies for controlling the production of those aromas in winemaking, through management of the principal control factors: YAN content and temperature during the alcoholic fermentation.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Jean-Roch Mouret*, Cristian Trelea, Jean-Marie Sablayrolles, Vincent Farines

*INRA

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Analysis of peptide fraction from white wines

Among nitrogen compounds included in white wines, the peptide fraction is certainly the least studied, however this fraction is quantitatively the most important (Feuillat, 1974). Existing studies concern the fraction below 1 kDa and only for white and sparkling wines (Bartolomé et al, 1997, Desportes et al 2000). In this report, we have developed methods to isolate peptides from reference white wines. Then, we have applied this methodology with bitter wine to answer a research question: is there a relation between peptides and the bitterness of white wine as for some cheese for example (Furtado, 1984)?

Effect of post-harvest ozone treatments on the skin phenolic composition and extractability of red winegrapes cv Nebbiolo and Barbera

Wine industry is looking forward for innovative, safe and eco-friendly antimicrobial products allowing the reduction of chemical treatments in the grape defense and the winemaking process that can affect negatively the quality of the product. Ozone has been tested in food industry giving good results in preventing fungi and bacteria growth on a wide spectrum of vegetables and fruits, due to its oxidant activity and ability to attack numerous cellular constituents. Ozone leaves no chemical residues on the food surface, decomposing itself rapidly in oxygen. Gaseous ozone has been already tested for table grapes storage and on wine grapes during withering.

Modulating role of SO2 in white wine protein haze formation

Despite the extensive research performed during the last decades, the multifactorial mechanism responsible for the white wine protein haze formation is not fully characterized. Herein, a new model is proposed, which is based on the experimental identification of sulfur dioxide as a major modulating factor inducing wine protein haze upon heating. As opposed to other reducing agents, such as 2-mercaptoethanol, dithiothreitol and tris(2-carboxyethyl)phosphine hydrochloride (TCEP), the addition of SO2 to must/wine upon heating cleaves intraprotein disulfide bonds, hinders thiol-disulfide exchange during protein interactions and can lead to the formation of novel inter/intraprotein disulfide bonds. Those are eventually responsible for wine protein aggregation which follows a nucleation-growth kinetic model as shown by dynamic light scattering [1].

Attractiveness and sweetness of red wines: Synergies between American oak barrels and mannoproteins

In partnership with a Bordeaux property wanting to improve the quality of its second wine, the effects of two factors, American oak barrels and mannoproteins were studied. Their impact on the attractiveness and sweetness of wines were characterized during two successive vintages (2012 and 2013). Vinification took place with a homogeneous batch of Cabernet Sauvignon. The wine was then divided up into various groups of five barrels of French and American oak, new or reused. Analyses of volatile and non-volatile wood compounds were undertaken at four months and eight months of wood ageing, by LC-MS and GC-MS.

Effect of ageing with Specific Inactivated Dry Yeasts on the volatile composition of Sauvignon Blanc and Carménère wines

Úbeda-Aguilera, C a, b, Peña-Neira, A.b Del Barrio-Galán, R.b, c a Biomedical Sciences Institute, Science Faculty, Universidad Autónoma de Chile, Chile. b Department of Agro-Industry and Enology, Faculty of Agronomical Sciences, University of Chile, Post Office Box 1004, Santa Rosa 11315, La Pintana, Santiago, Chile c Lallemand Inc. Chile y Compañía Limitada, Rosario Norte 407, piso 6, Las Condes, Santiago, Chile The wine is a complex matrix made up of several compounds which can interact among themselves throughout the wine ageing process, thereby modifying their sensorial characteristics. It is well known that during ageing of wines on lees, polysaccharides (mainly mannoproteins) can be released and can interact with the aromatic fraction modifying its volatility.