Macrowine 2021
IVES 9 IVES Conference Series 9 Spontaneous fermentation dynamics of indigenous yeast populations and their effect on the sensory properties of Riesling

Spontaneous fermentation dynamics of indigenous yeast populations and their effect on the sensory properties of Riesling

Abstract

Varietal Riesling aroma relies strongly on the formation and liberation of bound aroma compounds. Floral monoterpenes, green C6-alcohols, fruity C13-norisoprenoids and spicy volatile phenols are predominantly bound to disaccharides, which are produced and stored in the grape berry during berry maturation. Grape processing aims to extract maximum amount of the precursors from the berry skin to increase the potential for a strong varietal aroma in the wine. Subsequent yeast selection plays an important part in this process. While Saccharomyces cerevisiae seems to be limited in their expression of glycosidase enzymes required to cleave and liberate the aroma compounds from their precursors, different non-Saccharomyces yeast genera show more prominence. Due to the infrequent and scarce occurrence of Saccharomyces cerevisiae in the vineyard and grape samples, many scientific findings report that spontaneous alcoholic fermentation is dominantly conducted by yeast strains originating from the winery environment rather than from the vineyard. However, recent advancements of modern genetic tools have elucidated site-specific microbiota on grapes from different vineyards and vintages. Their role in fermented wine has not yet been clarified. This study aims to shed light on the roles of vineyard and winery microbiomes in wine fermentations in relation to fermentation dynamics, aroma formation and sensory perception. Riesling grapes from five different Riesling vineyards in the Pfalz region, Germany were picked aseptically during the 2015 vintage. Pilot-scale spontaneous fermentations with triplicates were conducted with aseptically managed winemaking. Fermentation progress was monitored by density measurements and FTIR-spectroscopy. Yeast population dynamics in the fermentations were monitored and identified with next-generation sequencing technology. Descriptive analysis of the wines was used to evaluate the changes in aroma and flavor sensory profiles. Results show plausible promise in both the microbial difference occurring in the vineyard as well as impact of the winery derived microbiome. Modulation of aroma and taste was observed and correlated with the occurrence of specific yeast species.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Kimmo Sirén*, M Thomas P Gilbert, Sarah S.T. Mak, Ulrich Fischer

*DLR RheinPfalz

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Anti/prooxidant activity of wine polyphenols in reactions of adrenaline auto-oxidation

Adrenaline (epinephrine) belongs to catecholamine class. It is a neurotransmitter and both a hormone which is released by the sympathetic nervous system and adrenal medulla in response to a range of stresses in order to regulate blood pressure, cardiac stimulation, relaxation of smooth muscles and other physiological processes. Adrenaline exhibits an effective antioxidant capacity (1). However, adrenalin is capable to auto-oxidation and in this case it generates toxic reactive oxygen intermediates and adrenochrome. Under in vitro conditions, auto-oxidation of adrenaline occurs in an alkaline medium (2).

Comparative proteomic analysis of wines made from Botrytis cinerea infected and healthy grapes reveal interesting parallels to the gushing phenomenon in sparkling wine

In addition to aroma compounds also protein composition strongly influences the quality of wines. Proteins of wine derive mainly from the plant Vitis vinifera and may be influenced by abiotic stress as well as fermentation conditions or fining. Additionally, fungal infections can affect the protein content as well by introducing fungal proteins or affecting grape protein composition. An infection of the vine with the plant pathogenic fungus Botrytis (B.) cinerea was shown to cause a degradation of proteins in the resulting wine. Moreover, it influences the foaming properties in sparkling wine.

Identification of green, aggressive and hard character of wines by a chemo-sensory directed methodology

With climate change, it is progressively more often to obtain grapes with an acceptable content in sugars or acids but with immature tannins described as green, aggressive or hard (noted as GAH onwards). During winemaking, the oenologist has to make decisions related to the elaboration of such grapes based mainly on empirical experience, given the lack of objective criteria to this concern. An increase in the chemical and sensory knowledge of immature tannins would allow managing this GAH character of grapes with the maximum possible efficiency during winemaking processes. The present work aims at isolating and identifying the group of compounds responsible for the GAH character present in wines.

The effect of cropload on the volatile aroma characteristics of ‘Beihong’ and ‘Beimei’ red wine

Beihong and Beimei were bred as winemaking cultivars released by Institute of Botany, the Chinese Academy of Sciences in 2008. The cultivars are selected from the population of ‘Muscat Hamburg’ (Vitis vinifera) ×V. amurensis. They are extended to most provinces in North of China because they have strong resistance to cold and disease and need not be buried in soil in winter. To better understand the effect of cropload on volatile compounds during wine-making, we surveyed volatiles composition and content of different cropload level in 3-years-old ‘Beihong’ and ‘Beimei’ vines which planted in east foot of Helan mountain of Ningxia (EHN).

Characterization of Glycosidically Bound Aroma Compounds of País cv. grapes of different Chilean zones

País grape has been estimated to arrive to Chile almost 500 years ago, being the first strain grown in this country. Traditionally, this grape has been used to mix with other varieties, to produce poor quality wines, but today is beginning to be used in the production of high quality wines. However, very little is known about the chemical characteristics of this variety. The aroma is one of the most important quality attributes of wine. Volatile compounds of this beverage may come from the grape (varietal aromas), from the fermentation process, from the ageing. The aromatic compounds are found in the grape in two forms: as free volatile compounds and as non-volatile compounds. The last ones, are aroma precursors present mainly as glycoconjugates formed by a sugar and an aglycone…