Macrowine 2021
IVES 9 IVES Conference Series 9 Spontaneous fermentation dynamics of indigenous yeast populations and their effect on the sensory properties of Riesling

Spontaneous fermentation dynamics of indigenous yeast populations and their effect on the sensory properties of Riesling

Abstract

Varietal Riesling aroma relies strongly on the formation and liberation of bound aroma compounds. Floral monoterpenes, green C6-alcohols, fruity C13-norisoprenoids and spicy volatile phenols are predominantly bound to disaccharides, which are produced and stored in the grape berry during berry maturation. Grape processing aims to extract maximum amount of the precursors from the berry skin to increase the potential for a strong varietal aroma in the wine. Subsequent yeast selection plays an important part in this process. While Saccharomyces cerevisiae seems to be limited in their expression of glycosidase enzymes required to cleave and liberate the aroma compounds from their precursors, different non-Saccharomyces yeast genera show more prominence. Due to the infrequent and scarce occurrence of Saccharomyces cerevisiae in the vineyard and grape samples, many scientific findings report that spontaneous alcoholic fermentation is dominantly conducted by yeast strains originating from the winery environment rather than from the vineyard. However, recent advancements of modern genetic tools have elucidated site-specific microbiota on grapes from different vineyards and vintages. Their role in fermented wine has not yet been clarified. This study aims to shed light on the roles of vineyard and winery microbiomes in wine fermentations in relation to fermentation dynamics, aroma formation and sensory perception. Riesling grapes from five different Riesling vineyards in the Pfalz region, Germany were picked aseptically during the 2015 vintage. Pilot-scale spontaneous fermentations with triplicates were conducted with aseptically managed winemaking. Fermentation progress was monitored by density measurements and FTIR-spectroscopy. Yeast population dynamics in the fermentations were monitored and identified with next-generation sequencing technology. Descriptive analysis of the wines was used to evaluate the changes in aroma and flavor sensory profiles. Results show plausible promise in both the microbial difference occurring in the vineyard as well as impact of the winery derived microbiome. Modulation of aroma and taste was observed and correlated with the occurrence of specific yeast species.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Kimmo Sirén*, M Thomas P Gilbert, Sarah S.T. Mak, Ulrich Fischer

*DLR RheinPfalz

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

The impact of branched chain and aromatic amino acids on fermentation kinetics and aroma biosynthesis by wine yeast Saccharomyces cerevisiae

One of the major determinants of wine quality is the aroma. Wine aroma is the human perception of the matrix of grape and yeast derived volatiles and their interaction that contribute to flavour wine. Most common are higher alcohols, ester and aldehydes. In previous studies the formation of characteristic volatile compounds have been linked to the metabolism of branched-chain and aromatic amino acids
(BCAAs) in synthetic grape must. Here we report on an investigation to assess the impact of the initial amino acid concentration on the production of aroma compounds by the industrial yeast VIN13 grown in both synthetic and real grape musts.

Defining the mechanisms and impact of winemaking treatments on tannin and polysaccharides in red wine: recent progress in creating diverse styles

Tannin and polysaccharide concentration and composition is important in defining the texture of red wines, but can vary due to factors such as cultivar, region, grape ripeness, viticultural practices and winemaking techniques. However, the concentration and composition of these macromolecules is dependent not only on grape tannin and polysaccharide concentration and composition, but also their extractability and, in the case of polysaccharides, their formation by yeast. Through studies into the influence of grape maturity, winemaking and sensory impacts of red grape polysaccharides, seed and skin tannins, recent research in our laboratory has shown that the processes involved in the extraction of these macromolecules from grapes and their retention in wine are very complex.

Comparative proteomic analysis of wines made from Botrytis cinerea infected and healthy grapes reveal interesting parallels to the gushing phenomenon in sparkling wine

In addition to aroma compounds also protein composition strongly influences the quality of wines. Proteins of wine derive mainly from the plant Vitis vinifera and may be influenced by abiotic stress as well as fermentation conditions or fining. Additionally, fungal infections can affect the protein content as well by introducing fungal proteins or affecting grape protein composition. An infection of the vine with the plant pathogenic fungus Botrytis (B.) cinerea was shown to cause a degradation of proteins in the resulting wine. Moreover, it influences the foaming properties in sparkling wine.

Cover crops influence on soil N availability and grapevine N status, and its relationship with biogenic

The type of soil management, tillage versus cover crops, can modify the soil microbial activity, which causes the mineralization of organic N to NO3–N and, therefore, may change the soil NO3–N availability in vineyard. The soil NO3–N availability could influence the grapevine nutritional status and the grape amino acid composition. Amino acids are precursors of biogenic amines, compounds mainly formed during the malolactic fermentation. Biogenic amines have negative effects on consumer health and on the wine organoleptic quality. The objective was to study if the effect of conventional tillage and two different cover crops (leguminous versus gramineous) on grapevine N status, could relate to the wine biogenic amines composition.

Characterization of commercial enological tannins and its effect on human saliva diffusion

Commercial oenological tannins (TECs) are widely used in the wine industry. TECs are rich in condensed tannins, hydrolyzable tannins or a mixture of both. Wine grapes are a important source of proanthocyanidins or condensed tannins while oak wood possess a high concentration of hydrolyzable tannins (Obreque-Slier et al., 2009). TECs contribute with the antioxidant capacity of wine, catalyze oxide-reduction reactions and participate in the removal of sulfur compounds and metals.