Macrowine 2021
IVES 9 IVES Conference Series 9 Characterization of commercial enological tannins and its effect on human saliva diffusion

Characterization of commercial enological tannins and its effect on human saliva diffusion

Abstract

Commercial oenological tannins (TECs) are widely used in the wine industry. TECs are rich in condensed tannins, hydrolyzable tannins or a mixture of both. Wine grapes are a important source of proanthocyanidins or condensed tannins while oak wood possess a high concentration of hydrolyzable tannins (Obreque-Slier et al., 2009). TECs contribute with the antioxidant capacity of wine, catalyze oxide-reduction reactions and participate in the removal of sulfur compounds and metals. One of the most important properties of TECs correspond to its contribution to the astringency (Zamora, 2003). Astringency, a sensation that is described as a puckering, rough, or drying mouth-feel, has been associated with interactions between some phenolic compounds (tannins) and salivary proteins (Bacon and Rhodes, 2000). A wide spectrum of enological tannins is now available on the market, classified mainly according to the enological properties. However, the tannins’ chemical nature is not always clearly defined. Furthermore, the effect of these on saliva is unknown. For that reason, the aim of this work was the chemical characterization of eleven commercial tannins sold for enological use. Likewise, we examined the effect of TECs on a physicochemical property of the salivary protein, namely, the mode of diffusion on cellulose membranes (Obreque-Slier et al., 2010). In this study, eleven enological tannins were characterized by classification into three groups according HPLC-DAD chromatography and spectroscopic analysis: enological products composed of proanthocyanidins, hydrolyzable, and the mixture of both types of tannins. Within each group, tannin composition varied greatly, mainly defined by the botanical origin of each commercial product. Similarly, when saliva was mixed with aliquots of increasing concentrations of TECs, we observed a progressive decrease in the blue-stained background of the distribution area of the salivary protein. The intensity of this restriction was in close relationship with the type of TECs. Finally, it was observed that certain TECs do not showed a effect on saliva diffusion on cellulose membranes.

References 1.- Bacon J., Rhodes M. 2000. Binding affinity of hydrolyzable tannins to parotid saliva and to proline-rich proteins derived from it. J Agric Food Chem 48, 838-843. 2.- Obreque-Slier E., Peña-Neira A., López-Solís R., Ramírez-Escudero C., Zamora-Marín F. 2009. Phenolic characterization of commercial enological tannins. Eur Food Res Technol 229, 859-866. 3.- Obreque-Slier E., Peña-Neira A., López-Solís, R. 2010. Quantitative determination of interactions between a tannin and a model protein using diffusion and precipitation assays on cellulose membranes. J Agric Food Chem 58, 8375-8379. 4.- Zamora, F. 2003. Elaboración y crianza del vino tinto: Aspectos científicos y prácticos. Madrid, España. Ediciones Mundi Prensa. 225p. Acknowledgments This study was supported by grant Fondecyt-Chile 1150240.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Elías Obreque Slier*, Álvaro Peña-Neira, Dante Munoz, Gina Vazallo, Marcela Medel, Remigio López

*Universidad de Chile

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

A preliminary study of clonal selection in cv. Viura in relation to varietal aroma profile

Viura is a synonym for Macabeo and currently it is the most widely planted white grape variety in D.O.Ca. Rioja, with 3,569 ha, representing 84% of the white grape cultivated area. It is a generous-yielding grape, presenting low values of titratable acidity and with large and compact clusters which makes it susceptible to Botrytis cinerea. Thus, this variety not always satisfies the wine grower’s prospects. Nowadays, the available plant material is scarce, moreover, it was selected on the basis of other quality criteria, not currently requested.

Sensory impacts of the obturator used for the Chasselas: study over the time

Many parameters affect the organoleptic characteristics of wine: internal parameters like the chemical composition or polyphenol content and external as for example storage conditions or the type of obturator. The aim of this study was to characterize sensorally the impacts of several type of obturator on a white wine: Chasselas. To determine the organoleptic characteristics of this wine, a quantitative descriptive analysis could be used. But rapid sensory methods were preferred in this project. Indeed these methods are an appropriate alternative to conventional descriptive methods for quickly assessing sensory product discrimination.

Ellagitannins and flavano-ellagitannins: concentration ranges in different areas and sensory evaluation

C-Glucosidic ellagitannins, which are the main polyphenolic compounds in oak heartwood, are extracted by wine during aging in oak barrels. Although such maturing of alcoholic beverages in oak barrels is a multi-centennial practice, very little is known on the impact of these ellagitannins on the organoleptic properties of red wine. The objectives of the present investigation were (i) to isolate oak ellagitannins and to hemisynthesize some made-in-wine flavano-ellagitannins, such as acutissimin A; (ii) to analyse their concentration ranges depending on the cultivar area and (iii) to evaluate their sensory impact on the basis of their human threshold concentrations and dose/response relationships in different types of solutions.

Assessing the effect of oak derived aromas on mouthfeel perception in Chardonnay wine

Mouthfeel is an important quality parameter for Chardonnay wines, particularly those aged in oak. While research on mouthfeel has traditionally focused on the impact of non-aromatic compounds, the role of aroma compounds has largely been over looked. However, in wine as well as other food interactions between retronasal aroma and mouthfeel have been noted. The goal of this research was to investigate the impact of wine aroma on the perception of mouthfeel. Because of the importance of oak aging in the development of Chardonnay mouthfeel, the impact of oak aromas on perceived mouthfeel was explored. Aroma compounds associated with oak (ethyl palmitate, eugenol, furfural, isoeugenol, syringaldehyde, vanillin and whiskey lactone) were added to two different Chardonnay wines; one with no oak influence and one fermented in neutral oak. Low and high concentrations of the compounds were added based on concentrations typically found in barrel aged Chardonnay wine.

Impact of heating must before fermentation on Chardonnay wines

Prefermentation steps of white winemaking are very important for controlling the stability and the sensory attributes of wines. Usually musts are clarified by cold settling to prevent the start of the fermentation, before racking big lees and thus limiting the appearance of vegetable or reduction off flavour while favouring an aromatic expression with low turbidity. Besides, to reach the protein stability, some white wines further require a bentonite fining, sometimes associated with negative effects on the sensory quality. This study aims to know the impact of musts heating after pressing on a Chardonnay wine in northern conditions by comparison with a classic cold racking of the must.