Macrowine 2021
IVES 9 IVES Conference Series 9 Characterization of commercial enological tannins and its effect on human saliva diffusion

Characterization of commercial enological tannins and its effect on human saliva diffusion

Abstract

Commercial oenological tannins (TECs) are widely used in the wine industry. TECs are rich in condensed tannins, hydrolyzable tannins or a mixture of both. Wine grapes are a important source of proanthocyanidins or condensed tannins while oak wood possess a high concentration of hydrolyzable tannins (Obreque-Slier et al., 2009). TECs contribute with the antioxidant capacity of wine, catalyze oxide-reduction reactions and participate in the removal of sulfur compounds and metals. One of the most important properties of TECs correspond to its contribution to the astringency (Zamora, 2003). Astringency, a sensation that is described as a puckering, rough, or drying mouth-feel, has been associated with interactions between some phenolic compounds (tannins) and salivary proteins (Bacon and Rhodes, 2000). A wide spectrum of enological tannins is now available on the market, classified mainly according to the enological properties. However, the tannins’ chemical nature is not always clearly defined. Furthermore, the effect of these on saliva is unknown. For that reason, the aim of this work was the chemical characterization of eleven commercial tannins sold for enological use. Likewise, we examined the effect of TECs on a physicochemical property of the salivary protein, namely, the mode of diffusion on cellulose membranes (Obreque-Slier et al., 2010). In this study, eleven enological tannins were characterized by classification into three groups according HPLC-DAD chromatography and spectroscopic analysis: enological products composed of proanthocyanidins, hydrolyzable, and the mixture of both types of tannins. Within each group, tannin composition varied greatly, mainly defined by the botanical origin of each commercial product. Similarly, when saliva was mixed with aliquots of increasing concentrations of TECs, we observed a progressive decrease in the blue-stained background of the distribution area of the salivary protein. The intensity of this restriction was in close relationship with the type of TECs. Finally, it was observed that certain TECs do not showed a effect on saliva diffusion on cellulose membranes.

References 1.- Bacon J., Rhodes M. 2000. Binding affinity of hydrolyzable tannins to parotid saliva and to proline-rich proteins derived from it. J Agric Food Chem 48, 838-843. 2.- Obreque-Slier E., Peña-Neira A., López-Solís R., Ramírez-Escudero C., Zamora-Marín F. 2009. Phenolic characterization of commercial enological tannins. Eur Food Res Technol 229, 859-866. 3.- Obreque-Slier E., Peña-Neira A., López-Solís, R. 2010. Quantitative determination of interactions between a tannin and a model protein using diffusion and precipitation assays on cellulose membranes. J Agric Food Chem 58, 8375-8379. 4.- Zamora, F. 2003. Elaboración y crianza del vino tinto: Aspectos científicos y prácticos. Madrid, España. Ediciones Mundi Prensa. 225p. Acknowledgments This study was supported by grant Fondecyt-Chile 1150240.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Elías Obreque Slier*, Álvaro Peña-Neira, Dante Munoz, Gina Vazallo, Marcela Medel, Remigio López

*Universidad de Chile

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Impact of sulfur compounds to the antioxidant stability of white wines

The chemical mechanisms involved in oxidation/reduction potential of wine during winemaking and aging are affecting its color, aroma and taste. Chemical oxidation is one of the major causes of development of off-flavors during ageing1. Thus, the chemical changes in wine during storage should be controlled to ensure the sensory quality of the product and avoid consumer rejection that will compromise the economic value of the product. The 1-hydroxyethyl radical has been recognized as the key radical intermediate in the oxidative reactions in wine2. Based on the kinetic study of POBN-1-hydroxyethyl spin adduct formation in wines initiated via the Fenton reaction, a novel tool was recently developed in our laboratory to quantify the resistance of wines against oxidation3.

Partial dealcoholisation of red wine by reverse osmosis-evaporative perstraction: impact on wine composition

Around the world, the alcohol content of wine has been steadily increasing; partly as a consequence of climate change, but also due to improvements in viticultural management practices and winemaking techniques [1,2]. Concurrently, market demand for wines with lower alcohol levels has increased as consumers seek to reduce alcohol intake for social and/or health reasons [3]. As such, there is increasing demand for both innovative methods that allow winemakers to produce ‘reduced alcohol wines’ (RAW) and a better understanding of the impact of such methods on the composition of RAW. This study therefore aimed to investigate compositional changes in two red wines resulting from partial alcohol removal following treatment by one such method, involving a combination of reverse osmosis and evaporative perstraction (RO-EP).

How do different oak treatment affect the sensory composition of Chenin blanc wines over time?

Wooden barrels have been the preferred method for oak maturation for wines, but the use of alternative oak products, such as staves and oak chips have increased in South Africa due to lower production costs. This study investigated the effect of different oak products used during fermentation and ageing on the sensory profile, degree of liking and perceived quality of a South African Chenin blanc wine. The different wine treatments included an unoaked tank control wine, wines matured in 5th fill barrels, wines matured in new barrels from three different cooperages, and wines matured in 5th fill barrels with stave inserts from two different cooperages.

Interactions of wine polyphenols with dead or living Saccharomyces cerevisiae Yeast Cells and Cell Walls: polyphenol location by microscopy

Tannin, anthocyanins and their reaction products play a major role in the quality of red wines. They contribute to their sensory characteristics, particularly colour and astringency. Grape tannins and anthocyanins are extracted during red wine fermentation. However, their concentration and composition change over time, due to their strong chemical reactivity1. It is also well known that yeasts influence the wine phenolic content, either through the release of metabolites involved in the formation of derived pigments1, or through polyphenol adsorption2,3.

Effect of mixed Torulaspora delbrueckii-Saccharomyces cerevisiae culture on rose quality wine

Alcoholic fermentation using no Saccharomyces wine is an effective means of modulating wine aroma. This study investigated the impact of coinoculating Torulaspora delbruecki with two Saccharomyces cerevisiae commercial yeast (QA23, Lallemand; Red Fruit, Sepsa-Enartis) on enological quality parameters, volatile composition and sensory analysis. The following assays were performed on Tempranillo variety: Saccharomyces QA23 (CTQA), Saccharomyces Red Fruit (CTRF), coinoculated T. delbrueckii + S.cerevisiae QA23 (CIQA) and coinoculated T. delbrueckii + S.cerevisiae (CIRF).