Macrowine 2021
IVES 9 IVES Conference Series 9 Characterization of commercial enological tannins and its effect on human saliva diffusion

Characterization of commercial enological tannins and its effect on human saliva diffusion

Abstract

Commercial oenological tannins (TECs) are widely used in the wine industry. TECs are rich in condensed tannins, hydrolyzable tannins or a mixture of both. Wine grapes are a important source of proanthocyanidins or condensed tannins while oak wood possess a high concentration of hydrolyzable tannins (Obreque-Slier et al., 2009). TECs contribute with the antioxidant capacity of wine, catalyze oxide-reduction reactions and participate in the removal of sulfur compounds and metals. One of the most important properties of TECs correspond to its contribution to the astringency (Zamora, 2003). Astringency, a sensation that is described as a puckering, rough, or drying mouth-feel, has been associated with interactions between some phenolic compounds (tannins) and salivary proteins (Bacon and Rhodes, 2000). A wide spectrum of enological tannins is now available on the market, classified mainly according to the enological properties. However, the tannins’ chemical nature is not always clearly defined. Furthermore, the effect of these on saliva is unknown. For that reason, the aim of this work was the chemical characterization of eleven commercial tannins sold for enological use. Likewise, we examined the effect of TECs on a physicochemical property of the salivary protein, namely, the mode of diffusion on cellulose membranes (Obreque-Slier et al., 2010). In this study, eleven enological tannins were characterized by classification into three groups according HPLC-DAD chromatography and spectroscopic analysis: enological products composed of proanthocyanidins, hydrolyzable, and the mixture of both types of tannins. Within each group, tannin composition varied greatly, mainly defined by the botanical origin of each commercial product. Similarly, when saliva was mixed with aliquots of increasing concentrations of TECs, we observed a progressive decrease in the blue-stained background of the distribution area of the salivary protein. The intensity of this restriction was in close relationship with the type of TECs. Finally, it was observed that certain TECs do not showed a effect on saliva diffusion on cellulose membranes.

References 1.- Bacon J., Rhodes M. 2000. Binding affinity of hydrolyzable tannins to parotid saliva and to proline-rich proteins derived from it. J Agric Food Chem 48, 838-843. 2.- Obreque-Slier E., Peña-Neira A., López-Solís R., Ramírez-Escudero C., Zamora-Marín F. 2009. Phenolic characterization of commercial enological tannins. Eur Food Res Technol 229, 859-866. 3.- Obreque-Slier E., Peña-Neira A., López-Solís, R. 2010. Quantitative determination of interactions between a tannin and a model protein using diffusion and precipitation assays on cellulose membranes. J Agric Food Chem 58, 8375-8379. 4.- Zamora, F. 2003. Elaboración y crianza del vino tinto: Aspectos científicos y prácticos. Madrid, España. Ediciones Mundi Prensa. 225p. Acknowledgments This study was supported by grant Fondecyt-Chile 1150240.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Elías Obreque Slier*, Álvaro Peña-Neira, Dante Munoz, Gina Vazallo, Marcela Medel, Remigio López

*Universidad de Chile

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Characterization of free and glycosidically bound simple phenols in hybrid grape varieties using liquid chromatography coupled to high resolution mass (q-orbitrap)

Vitis vinifera is one of the most diffused grapevines over the word and it is the raw material for high quality wines production. The availability of more resistant interspecific hybrid vine varieties, developed from crosses between Vitis vinifera and other Vitis species, has generating much interest, also due to the low environmental effect of production. However, hybrid grape wine composition and varietal differences between interspecific hybrids are not well defined. Different studies revealed that wine consumption has health effects due to its high content of antioxidants, as phenolic compounds. In particular, simple phenols are appreciated not only for their physiological health benefits, including antioxidant, anti-inflammatory and cardioprotective effects, but also because they affect wines organoleptic profile and have a significant role in defining their nutritional characteristics.

Impact of industrial-scale serial filtration on macromolecules in red wines

Filtration is a critical step in ensuring the clarity and microbial stability of wine prior to bottling. However the process of filtering potentially reduces red wine quality by removing some of the macromolecules that contribute to the texture of the wine. Commercial red wines, Cabernet Sauvignon (CAS) and Shiraz (SHZ), of two vintages and two grades (premium grade wines from the older vintage: CAS13 and SHZ13; and standard grade wines from a younger vintage: CAS14 and SHZ14) were filtered through industrial-scale commercial filtration units prior to bottling. Samples were taken before and after cross-flow filtration, lenticular filters, 0.65 µm and 0.45 µm pore size nylon membrane filters. The concentration and composition of macromolecules, including tannins and polysaccharides, were measured in all samples as well as particle size distribution and wine colour.

What about oxygen transfer during wine aging in barrels?

During wine aging, several complex phenomena of gas transfer take place in barrels due to the wine/oak contact. The efficiency of this gas transfer varies according to oak wood’s intrinsic physical properties. This research aims to better understand oxygen transfer phenomena through dry oak staves and especially through stave gaps, in order to reevaluate the importance of barrel-making on a barrel’s supply of oxygen. Experimentation was based on the development of an innovative permeameter of laboratory scale, for which the principal operating conditions concerning applied pressure, the choice of liquid phase/gas phase, and the grain type of oak are taken into account and investigated. With a specially developed tightening system, the existing pressure at stave gaps in a barrel could be reproduced on a laboratory scale in order to estimate its influence on oxygen transfer efficiency.

Assessing the effect of oak derived aromas on mouthfeel perception in Chardonnay wine

Mouthfeel is an important quality parameter for Chardonnay wines, particularly those aged in oak. While research on mouthfeel has traditionally focused on the impact of non-aromatic compounds, the role of aroma compounds has largely been over looked. However, in wine as well as other food interactions between retronasal aroma and mouthfeel have been noted. The goal of this research was to investigate the impact of wine aroma on the perception of mouthfeel. Because of the importance of oak aging in the development of Chardonnay mouthfeel, the impact of oak aromas on perceived mouthfeel was explored. Aroma compounds associated with oak (ethyl palmitate, eugenol, furfural, isoeugenol, syringaldehyde, vanillin and whiskey lactone) were added to two different Chardonnay wines; one with no oak influence and one fermented in neutral oak. Low and high concentrations of the compounds were added based on concentrations typically found in barrel aged Chardonnay wine.

An excessive leaf-fruit ratio reduces the yeast assimilable nitrogen in the must

Yeast assimilable nitrogen (YAN) in the grape must is a key variable for wine quality as a source of aroma precursors. In a situation of YAN deficiency, a foliar urea application upon the vine at veraison enhances YAN concentration and facilitates must fermentation. In 2013, Agroscope investigated the impact of leaf-fruit ratio on the nitrogen (N) assimilation and partitioning in grapevine Vitis vinifera cv. Chasselas following foliar-urea application with the aim of improving its efficiency on the YAN concentration.