Macrowine 2021
IVES 9 IVES Conference Series 9 Sensory definition of green aroma concept in red French wines. Evidence for the contribution of novel volatile markers

Sensory definition of green aroma concept in red French wines. Evidence for the contribution of novel volatile markers

Abstract

The aromatic complexity of a wine results from the perception of the association of volatile molecules and each aroma can be categorized into different families. The “green” aromas family in red wines has retained our attention by its close link with the fruity perception. In that study, the “green” olfactory concept of red wines was considered through a strategy combining both sensory analysis and hyphenated chromatographic techniques including HPLC and MDGC (Multidimensional Gas Chromatography). The aromatic space of this concept was specified by lexical generation through a free association task on 22 selected wines by a panel of wine experts. Then, 70 French red wines were scored on the basis of the intensity of their “green” and “fruity” attributes. The relationship between these two families and chemical markers (2-methoxy-3-isobutyl, 3-isopropyl and 3-sec-butyl pyrazines and C6 alcohol compounds) was assessed. Strong anti-correlation between “green” and “fruity” was demonstrated but no significant correlations could be established with known molecular markers associated with fresh green characters. So, the contribution of other aromatic compounds was formulated in particular with the level of grape maturation. Microvinifications of Cabernet-Sauvignon grapes from Medoc vineyard harvested at two ripeness stages were done during 2014 and 2015 vintages. The so-obtained wines were extracted with organic solvents and the concentrated extracts were fractioned by semi-preparative HPLC. Among fifty fractions collected, one was particularly highlighted for its green aromas. The sensory impact of this fraction was first confirmed by omission and reconstitution tests. To determine the molecules responsible for green flavour of this fraction, GC-O (Gas Chromatography coupled with Olfactometry) and MDGC-O/Time-Of-Flight-MS were considered. 2-Methoxy-3-isobutyl-pyrazine well known as IBMP (bell pepper descriptor) was identified as one of the compounds responsible for the aroma of this fraction with two other odoriferous compounds presenting a green aroma. One belongs to terpene family, 1,8-cineole (herbs, fresh and eucalyptus descriptors), usually associated in wines with eucalyptus trees contamination. 1,8-cineole was definitively evidenced as a Vitis vinifera compound with concentrations assayed in wines from unripe grapes and other wines from Carmenet family close or higher to olfactory detection threshold (1 µg/l). 1,8-cineole concentrations were shown to decrease like IBMP during ripeness. The other compound was identified as methyl salicylate (fresh, leafy and wintergreen descriptors), a derivative of salicylic acid, sometimes quantified in wine samples at concentrations much higher than its detection threshold (40 µg/l). Supplementation tests at assayed concentrations in red wines for these two compounds exhibited a sensory impact on green aromas and a synergic effect was noticed with IBMP on the enhancement of the green flavour in red wines.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Xavier Poitou*, Philippe Darriet

*ISVV

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

The effect of cropload on the volatile aroma characteristics of ‘Beihong’ and ‘Beimei’ red wine

Beihong and Beimei were bred as winemaking cultivars released by Institute of Botany, the Chinese Academy of Sciences in 2008. The cultivars are selected from the population of ‘Muscat Hamburg’ (Vitis vinifera) ×V. amurensis. They are extended to most provinces in North of China because they have strong resistance to cold and disease and need not be buried in soil in winter. To better understand the effect of cropload on volatile compounds during wine-making, we surveyed volatiles composition and content of different cropload level in 3-years-old ‘Beihong’ and ‘Beimei’ vines which planted in east foot of Helan mountain of Ningxia (EHN).

Quantification of the production of hydrogen peroxide H2O2 during wine oxidation

Chemical studies aiming at assessing how a wine reacts towards oxidation usually focus on the characterization of wine constituents, such as polyphenols, or oxidation products. As an alternative, the key oxidation intermediate hydrogen peroxide H2O2 has never been quantified, although it plays a pivotal role in wine oxidation. H2O2 is obtained from molecular oxygen as the result of a first cascade of oxidation reactions involving metal ions and polyphenols. The produced H2O2 then reacts in a second cascade of oxidation to produce reactive hydroxyl radicals that can attack almost any chemical substrate in wine.

Technological possibilities of grape marc cell walls as wine fining agent. Effect on wine phenolic composition

Fining is a technique that is used to remove unwanted wine components that affect clarification, astringency, color, bitterness, and aroma. Fining involves the addition of adsorptive or reactive material in order to reduce or eliminate the presence of certain less desirable wine components and to ensure that a wine remains in a particular stable state for a given period of time Recently concerns have been raised about the addition of animal proteins, such as gelatin, to wine due to the disease known as bovine spongiform encephalopathy (Mad Cow disease). Although the origin of gelatins has been moved to porcine, winemakers are asking for substitute products with properties and application protocols similar to the traditional animal-derived ones, making the use of plant-derived proteins in fining a practically viable possibility. As a consequence, various fining agents derived from plants have been proposed, including proteins from cereals, legumes, and potato.

Trans-resveratrol concentrations in wines Cabernet Sauvignon from Chile

This study evaluated the levels of trans-resveratrol in commercial wines made from Cabernet Sauvignon grapes from different valleys of Chile stilbenes. The Cabernet Sauvignon is the most planted variety in Chile, being 38% of the total vineyard country. Chile is the fourth largest wine exporter in the world, so it is important to evaluate the Cabernet-Sauvignon wines in their concentration levels of trans-resveratrol and its relation to the benefits provided to human health in moderate consumption. Evaluation comprises commercial wines from different valleys of Chile and its relationship with climatic characteristics, soil and vineyard handling.

Testing the effectiveness of Cell-Wall material from grape pomace as fining agent for red wines

Lately several works highlighted the capacity of grape cell-wall material (CWM) to interact with proanthocyanidins (PA), indicating its potential use as fining agent for red wines.1–4 However, those studies were performed by using purified PAs and very high doses of CWM (almost ten-fold higher than those used in wine industry for other commercial fining agents). The present study focuses on the applicability of CWM from Cabernet sauvignon pomace as fining agent for red wines under real winery conditions. Grapes of cultivar Cabernet sauvignon were harvested at three different maturity levels
(unripe, mature, and overripe) and used for red winemaking. The pomace of such vinifications were used as source of CWM, and applied into red wines at two different concentrations: 0.2 g/L and 2.5 g/L.