Macrowine 2021
IVES 9 IVES Conference Series 9 Sensory definition of green aroma concept in red French wines. Evidence for the contribution of novel volatile markers

Sensory definition of green aroma concept in red French wines. Evidence for the contribution of novel volatile markers

Abstract

The aromatic complexity of a wine results from the perception of the association of volatile molecules and each aroma can be categorized into different families. The “green” aromas family in red wines has retained our attention by its close link with the fruity perception. In that study, the “green” olfactory concept of red wines was considered through a strategy combining both sensory analysis and hyphenated chromatographic techniques including HPLC and MDGC (Multidimensional Gas Chromatography). The aromatic space of this concept was specified by lexical generation through a free association task on 22 selected wines by a panel of wine experts. Then, 70 French red wines were scored on the basis of the intensity of their “green” and “fruity” attributes. The relationship between these two families and chemical markers (2-methoxy-3-isobutyl, 3-isopropyl and 3-sec-butyl pyrazines and C6 alcohol compounds) was assessed. Strong anti-correlation between “green” and “fruity” was demonstrated but no significant correlations could be established with known molecular markers associated with fresh green characters. So, the contribution of other aromatic compounds was formulated in particular with the level of grape maturation. Microvinifications of Cabernet-Sauvignon grapes from Medoc vineyard harvested at two ripeness stages were done during 2014 and 2015 vintages. The so-obtained wines were extracted with organic solvents and the concentrated extracts were fractioned by semi-preparative HPLC. Among fifty fractions collected, one was particularly highlighted for its green aromas. The sensory impact of this fraction was first confirmed by omission and reconstitution tests. To determine the molecules responsible for green flavour of this fraction, GC-O (Gas Chromatography coupled with Olfactometry) and MDGC-O/Time-Of-Flight-MS were considered. 2-Methoxy-3-isobutyl-pyrazine well known as IBMP (bell pepper descriptor) was identified as one of the compounds responsible for the aroma of this fraction with two other odoriferous compounds presenting a green aroma. One belongs to terpene family, 1,8-cineole (herbs, fresh and eucalyptus descriptors), usually associated in wines with eucalyptus trees contamination. 1,8-cineole was definitively evidenced as a Vitis vinifera compound with concentrations assayed in wines from unripe grapes and other wines from Carmenet family close or higher to olfactory detection threshold (1 µg/l). 1,8-cineole concentrations were shown to decrease like IBMP during ripeness. The other compound was identified as methyl salicylate (fresh, leafy and wintergreen descriptors), a derivative of salicylic acid, sometimes quantified in wine samples at concentrations much higher than its detection threshold (40 µg/l). Supplementation tests at assayed concentrations in red wines for these two compounds exhibited a sensory impact on green aromas and a synergic effect was noticed with IBMP on the enhancement of the green flavour in red wines.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Xavier Poitou*, Philippe Darriet

*ISVV

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Wood from barrique: release of phenolic compounds and permeability to oxygen

Chemical and sensory changes occurring in red wine during ageing in oak barrique are due to the slow and gradual entrance of oxygen along with a release of ellagic tannin from the wood. Though oxygen can enter the cask through the bunghole, it is not clear the role of permeation through the wood staves as well as the amount of oxygen entering by permeation. The distribution of the released ellagic tannins in the wine ageing is also unknown. The oxygen passing through the bunghole may have a different wine ageing effect compared to the oxygen permeating through the wooden staves owing to the uneven ellagic tannin concentration throughout the wine.

Partial dealcoholisation of red wine by reverse osmosis-evaporative perstraction: impact on wine composition

Around the world, the alcohol content of wine has been steadily increasing; partly as a consequence of climate change, but also due to improvements in viticultural management practices and winemaking techniques [1,2]. Concurrently, market demand for wines with lower alcohol levels has increased as consumers seek to reduce alcohol intake for social and/or health reasons [3]. As such, there is increasing demand for both innovative methods that allow winemakers to produce ‘reduced alcohol wines’ (RAW) and a better understanding of the impact of such methods on the composition of RAW. This study therefore aimed to investigate compositional changes in two red wines resulting from partial alcohol removal following treatment by one such method, involving a combination of reverse osmosis and evaporative perstraction (RO-EP).

Prediction of the production kinetics of the main fermentative aromas in alcoholic fermentation

Fermentative aromas (especially esters and higher alcohols) highly impact the organoleptic profile of young and white wines. The production of these volatile compounds depends mainly on temperature and Yeast Available Nitrogen (YAN) content in the must. Available dynamic models predict the main reaction
(bioconversion of sugar into ethanol and CO2 production) but none of them considers the production kinetics of fermentative aroma compounds during the process of fermentation. We determined the production kinetics of the main esters and higher alcohols for different values of initial YAN content and temperature, using an innovative online monitoring Gas Chromatography device.

Grape byproducts as source of resveratrol oligomers for the development of antifungal extracts

Grape canes are a non-recycled byproduct of wine industry (1-5 tons per hectare per year) containing valuable phytochemicals of medicine and agronomical interest. Resveratrol and wine polyphenols are known to exert a plethora of health-promoting effects including antioxidant capacity, cardioprotection, anticancer activity, anti-inflammatory effects, and estrogenic/antiestrogenic properties (Guerrero et al. 2009). Additionally, resveratrol is a major phytoalexin produced by plants in response to various stresses and promotes disease resistance (Chang et al. 2011). Our project aims to develop polyphenol-rich grape cane extracts to fight phytopathogenic or clinically relevant fungi. We initiate the project with the development of analytical methods to analyze resveratrol mono- and oligomers (dimers, trimers and tetramers) from grape canes and we evaluate their potential activity against clinically relevant opportunistic fungal pathogens (Houillé et al. 2014).

Pesticide removal in wine with a physical treatment by molecular sieving

All along the winemaking process, conditioning and aging, wine is susceptible to be contaminated by different molecules. Contaminations can have various origins, related to wine microorganisms or as a result of an exogenous contamination. The aforementioned contamination of the wine can be caused by the migration of molecules from the materials in contact with the wine or by a contamination from exogenous molecules present in the air. Regardless of the source of the contamination, mainly two types of consequences can be observed.