Macrowine 2021
IVES 9 IVES Conference Series 9 Comparison of various storage conditions to preserve polyphenols in red-grape pomace

Comparison of various storage conditions to preserve polyphenols in red-grape pomace

Abstract

Red grape pomace, a waste from wine production, can be valorised by extracting polyphenols, high-added value compounds used in cosmetics or oenology. For use at an industrial level, using green extraction techniques, pomace need to be stored before being processed. The aim of this study is to test various storage conditions in order to maintain high level of polyphenols over 180 days, while keeping storage cost economically interesting. In a first step, different storage conditions (ambient temperature or cooled (4°C) temperature, anaerobic (saturation with N2) or aerobic conditions, and addition of sulphur dioxide (SO2)) were compared on small samples (1 kg) packed in plastic pockets. The quality of storage was assessed by following the optical density of the pomace extract at 280 nm (DO 280 expressed as mg/l eq gallic acid), which is an indication of the amount of remaining extractable polyphenols. The Colour Intensity (CI) (DO 420/520) was also measured as an indicator for oxidation. Organic acids (e.g. malic acid), sugars (e.g. glucose) alcohol, bacteria and yeasts were also quantified as an indicator for the occurrence of fermentation processes in the stored pomace. The results show that storage at 4°C under anaerobic conditions provides the best conditions to preserve polyphenols. Only little reduction of the polyphenol concentration, as well as little oxidation were observed. The addition of SO2 alone could not prevent a strong reduction of the polyphenol concentration in samples stored at ambient temperature. The preservation of pomace under ambient temperature, without protection against oxidation (N2 or SO2) induced a high activity of bacteria and yeasts measured by the reduction of sugar contents, and the transformation of alcohol in acetic acid. Even though refrigeration is efficient against polyphenol lost, at industrial scale it is costly. Therefore in a second step, storage under aerobic and anaerobic conditions (saturation with N2) at ambient temperature in opened or close containers were tested on larger size samples (700 l) to evaluate the effect of large volumes on storage. One assumption was that the core of the heap would be protected from oxidation. The results show that temperature measured in the pomace heap at a depth of 20 cm did not differ from temperature measured at 50 cm. It was confirmed by the monitoring of the fermentation processes which were identical at the surface and deep inside the heap. The assumption regarding protection of the heap core against oxidation was thus incorrect. A strong increase of temperature during the 60 first days of storage was observed under aerobic conditions indicating the occurrence of important fermentation processes. The best way to preserve high-added value compounds in large amount of red grape pomaces is to store it in a sealed tank saturated with N2. Under these conditions the fermentation processes were very limited and the polyphenols were protected from oxidation.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Anne-Claire Silvestri*, Jean-Philippe Burdet, Laure Steiner-Convers

*HES-SO

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Assessing the effect of oak derived aromas on mouthfeel perception in Chardonnay wine

Mouthfeel is an important quality parameter for Chardonnay wines, particularly those aged in oak. While research on mouthfeel has traditionally focused on the impact of non-aromatic compounds, the role of aroma compounds has largely been over looked. However, in wine as well as other food interactions between retronasal aroma and mouthfeel have been noted. The goal of this research was to investigate the impact of wine aroma on the perception of mouthfeel. Because of the importance of oak aging in the development of Chardonnay mouthfeel, the impact of oak aromas on perceived mouthfeel was explored. Aroma compounds associated with oak (ethyl palmitate, eugenol, furfural, isoeugenol, syringaldehyde, vanillin and whiskey lactone) were added to two different Chardonnay wines; one with no oak influence and one fermented in neutral oak. Low and high concentrations of the compounds were added based on concentrations typically found in barrel aged Chardonnay wine.

Evaluation of Polarized Projective Mapping as a possible tool for attributing South African Chenin blanc dry wine styles

Multiple Factor Analysis (MFA) According to the Chenin blanc Association of South Africa, there are three recognized dry wine styles, Fresh and Fruity (FF), Rich and Ripe Unwooded (RRU), and Rich and Ripe Wooded (RRW), classically attributed with the help of sensory evaluation. One of the “rapid methods” has drawn our attention for the purpose of simplifying and making style attribution for large sample sets, evaluated during different sessions, more robust. Polarized Projective Mapping (PPM) is a hybrid of Projective Mapping (PM) and Polarised Sensory Positioning (PSP). It is a reference-based method in which poles
(references) are used for the evaluation of similarities and dissimilarities between samples.

Crown procyanidin: a new procyanidin sub-family with unusual cyclic skeleton in wine

Condensed tannins (also called proanthocyanidins) are a widely distributed throughout in plants kingdom and are one of the most important classes of secondary metabolites, in addition, they are part of the human diet. In wine, they are extracted during the winemaking process from grape skins and seeds. These compounds play an important role in red wine organoleptic characteristics such as color, bitterness and astringency. Condensed tannins in red wine are oligomers and polymers of flavan-3-ols unit such as catechin, epicatechin, epigallocatechin and epicatechin-3-O-gallate. The monomeric units can be linked among them with direct interflavanoid linkage or mediated by aldehydes.

Bentonite fining in cold wines: prediction tests, reduced efficiency and possibilities to avoid additional fining treatments

Bentonite fining is widely used to prevent protein haze in white wines. Most wineries use laboratory-scale fining trials to define the appropriate amount of bentonite to be used in the cellar. Those pre-tests need to mimic as much as possible the industrial scale fining procedure to determine the exact amount of bentonite necessary for protein stability. Nevertheless it is frequent that, after fining with the recommended amount of bentonite, wines appear still unstable and need an additional fining treatment. It remains a major challenge to understand why the same wine, fined with the same dosage of the same bentonite, achieves stability in the lab, but not in the cellar.

Technological possibilities of grape marc cell walls as wine fining agent. Effect on wine phenolic composition

Fining is a technique that is used to remove unwanted wine components that affect clarification, astringency, color, bitterness, and aroma. Fining involves the addition of adsorptive or reactive material in order to reduce or eliminate the presence of certain less desirable wine components and to ensure that a wine remains in a particular stable state for a given period of time Recently concerns have been raised about the addition of animal proteins, such as gelatin, to wine due to the disease known as bovine spongiform encephalopathy (Mad Cow disease). Although the origin of gelatins has been moved to porcine, winemakers are asking for substitute products with properties and application protocols similar to the traditional animal-derived ones, making the use of plant-derived proteins in fining a practically viable possibility. As a consequence, various fining agents derived from plants have been proposed, including proteins from cereals, legumes, and potato.