Macrowine 2021
IVES 9 IVES Conference Series 9 Comparison of various storage conditions to preserve polyphenols in red-grape pomace

Comparison of various storage conditions to preserve polyphenols in red-grape pomace

Abstract

Red grape pomace, a waste from wine production, can be valorised by extracting polyphenols, high-added value compounds used in cosmetics or oenology. For use at an industrial level, using green extraction techniques, pomace need to be stored before being processed. The aim of this study is to test various storage conditions in order to maintain high level of polyphenols over 180 days, while keeping storage cost economically interesting. In a first step, different storage conditions (ambient temperature or cooled (4°C) temperature, anaerobic (saturation with N2) or aerobic conditions, and addition of sulphur dioxide (SO2)) were compared on small samples (1 kg) packed in plastic pockets. The quality of storage was assessed by following the optical density of the pomace extract at 280 nm (DO 280 expressed as mg/l eq gallic acid), which is an indication of the amount of remaining extractable polyphenols. The Colour Intensity (CI) (DO 420/520) was also measured as an indicator for oxidation. Organic acids (e.g. malic acid), sugars (e.g. glucose) alcohol, bacteria and yeasts were also quantified as an indicator for the occurrence of fermentation processes in the stored pomace. The results show that storage at 4°C under anaerobic conditions provides the best conditions to preserve polyphenols. Only little reduction of the polyphenol concentration, as well as little oxidation were observed. The addition of SO2 alone could not prevent a strong reduction of the polyphenol concentration in samples stored at ambient temperature. The preservation of pomace under ambient temperature, without protection against oxidation (N2 or SO2) induced a high activity of bacteria and yeasts measured by the reduction of sugar contents, and the transformation of alcohol in acetic acid. Even though refrigeration is efficient against polyphenol lost, at industrial scale it is costly. Therefore in a second step, storage under aerobic and anaerobic conditions (saturation with N2) at ambient temperature in opened or close containers were tested on larger size samples (700 l) to evaluate the effect of large volumes on storage. One assumption was that the core of the heap would be protected from oxidation. The results show that temperature measured in the pomace heap at a depth of 20 cm did not differ from temperature measured at 50 cm. It was confirmed by the monitoring of the fermentation processes which were identical at the surface and deep inside the heap. The assumption regarding protection of the heap core against oxidation was thus incorrect. A strong increase of temperature during the 60 first days of storage was observed under aerobic conditions indicating the occurrence of important fermentation processes. The best way to preserve high-added value compounds in large amount of red grape pomaces is to store it in a sealed tank saturated with N2. Under these conditions the fermentation processes were very limited and the polyphenols were protected from oxidation.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Anne-Claire Silvestri*, Jean-Philippe Burdet, Laure Steiner-Convers

*HES-SO

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Reduction of herbaceous aromas by wine lactic acid bacteria mediated degradation of volatile aldehydes

Consumers typically prefer wines with floral and fruity aromas over those presenting green-pepper, vegetal or herbaceous notes. Pyrazines have been identified as causatives for herbaceous notes in wines, especially Bordeaux reds. However, pyrazines are not universally responsible for herbaceousness, and several other wine volatile compounds are known to produce distinct vegetal/herbaceous aromas in wines. Specifically, volatile aldehydes elicit sensations of herbaceousness or grassiness and have been described in wines well above their perception thresholds.

How pressing techniques affect must composition and wine quality of Pinot blanc

This study investigates how the sensory profile of Pinot Blanc is affected from different maceration and pressing techniques. Grapes were sourced from four vineyards in the village Tramin in South Tyrol. For the experiment 200 kg of grapes from each vineyard site were hand picked the day before harvest for the commercial winery took place. Grapes were stored over night at 4°C, homogenized and processed in the experimental winery at Laimburg research centre the day after harvest. Four different pressing techniques were applied in duplicates of 100kg each.

Extraction of polyphenols from grape marc by supercritical fluid extraction (SFE) and evaluation of their ‘bioavailability’ as dietary supplements

In the winemaking process, several compounds that remain in the grape skins and seeds after the fermentation stage are bioactive-compounds (substances with potential beneficial effects on health) that can be extracted in order to recovery valuable substances with a high commercial value for the cosmetic, food (nutraceuticals) and pharmaceutical industries. The skins contain significant amounts of bioactive substances such as tannins (16-27%) and other polyphenolic compounds (2-6.5%) in particular, catechins, anthocyanins, proanthocyanins, quercetin , ellagic acid and resveratrol.

IBMP-Polypenol interactions: Impact on volatility and sensory perception in model wine solution

3-Isobutyl-2-methoxypyrazine (IBMP) is one of the key molecules in wine aroma with a bell pepper aroma and a very low threshold in wine, 1-6 ng/L for white wine and 10-16 ng/L in red wine1. The differences in these thresholds are likely due to IBMP-non volatile matrix interactions. It has indeed been shown that polyphenols may influence the volatility of flavor compounds2. In the present study, we focus on IBMP-polyphenols interactions in relation to volatility and sensory perception in model wine solution. Methods: 1. GC-MS Static Headspace Analysis: Samples were analyzed by Static headspace analysis with an Agilent 7890A gas chromatograph coupled to HP 5975C mass spectrometry detector (Agilent Technologies, Santa Clara, CA, USA).

Identification of green, aggressive and hard character of wines by a chemo-sensory directed methodology

With climate change, it is progressively more often to obtain grapes with an acceptable content in sugars or acids but with immature tannins described as green, aggressive or hard (noted as GAH onwards). During winemaking, the oenologist has to make decisions related to the elaboration of such grapes based mainly on empirical experience, given the lack of objective criteria to this concern. An increase in the chemical and sensory knowledge of immature tannins would allow managing this GAH character of grapes with the maximum possible efficiency during winemaking processes. The present work aims at isolating and identifying the group of compounds responsible for the GAH character present in wines.