Macrowine 2021
IVES 9 IVES Conference Series 9 Comparison of various storage conditions to preserve polyphenols in red-grape pomace

Comparison of various storage conditions to preserve polyphenols in red-grape pomace

Abstract

Red grape pomace, a waste from wine production, can be valorised by extracting polyphenols, high-added value compounds used in cosmetics or oenology. For use at an industrial level, using green extraction techniques, pomace need to be stored before being processed. The aim of this study is to test various storage conditions in order to maintain high level of polyphenols over 180 days, while keeping storage cost economically interesting. In a first step, different storage conditions (ambient temperature or cooled (4°C) temperature, anaerobic (saturation with N2) or aerobic conditions, and addition of sulphur dioxide (SO2)) were compared on small samples (1 kg) packed in plastic pockets. The quality of storage was assessed by following the optical density of the pomace extract at 280 nm (DO 280 expressed as mg/l eq gallic acid), which is an indication of the amount of remaining extractable polyphenols. The Colour Intensity (CI) (DO 420/520) was also measured as an indicator for oxidation. Organic acids (e.g. malic acid), sugars (e.g. glucose) alcohol, bacteria and yeasts were also quantified as an indicator for the occurrence of fermentation processes in the stored pomace. The results show that storage at 4°C under anaerobic conditions provides the best conditions to preserve polyphenols. Only little reduction of the polyphenol concentration, as well as little oxidation were observed. The addition of SO2 alone could not prevent a strong reduction of the polyphenol concentration in samples stored at ambient temperature. The preservation of pomace under ambient temperature, without protection against oxidation (N2 or SO2) induced a high activity of bacteria and yeasts measured by the reduction of sugar contents, and the transformation of alcohol in acetic acid. Even though refrigeration is efficient against polyphenol lost, at industrial scale it is costly. Therefore in a second step, storage under aerobic and anaerobic conditions (saturation with N2) at ambient temperature in opened or close containers were tested on larger size samples (700 l) to evaluate the effect of large volumes on storage. One assumption was that the core of the heap would be protected from oxidation. The results show that temperature measured in the pomace heap at a depth of 20 cm did not differ from temperature measured at 50 cm. It was confirmed by the monitoring of the fermentation processes which were identical at the surface and deep inside the heap. The assumption regarding protection of the heap core against oxidation was thus incorrect. A strong increase of temperature during the 60 first days of storage was observed under aerobic conditions indicating the occurrence of important fermentation processes. The best way to preserve high-added value compounds in large amount of red grape pomaces is to store it in a sealed tank saturated with N2. Under these conditions the fermentation processes were very limited and the polyphenols were protected from oxidation.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Anne-Claire Silvestri*, Jean-Philippe Burdet, Laure Steiner-Convers

*HES-SO

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Petrolomics-derived data interpretation to study acetaldehyde-epicatechin condensation reactions

During red wine ageing or conservation, color and taste change and astringency tends to reduce. These changes result from reactions of flavan-3-ols and/or anthocyanins among which condensation reactions with acetaldehyde are particularly important. The full characterization of these reactions has not been fully achieved because of difficulties in extracting and separating the newly formed compounds directly from wine. Model solutions mimicking food products constitute a simplified medium for their exploration, allowing the detection of the newly formed compounds, their isolation, and their structure elucidation.

DNA and type of grain: which factor does better explain sensory differences of sessile and pedunculate oaks?

Sessile oak and pedunculate oak have shown several differences of interest for enological purposes. Tannic and aromatic composition among sessile oak or pedonculate oak has been well studied. Sessile oak is generally more aromatic than pedunculated, while the later is more tannic. This scientific point of view is rarely applied to classify oak in cooperages. Most coopers use the type of grain to distinguish wide and thin grain.

Technological possibilities of grape marc cell walls as wine fining agent. Effect on wine phenolic composition

Fining is a technique that is used to remove unwanted wine components that affect clarification, astringency, color, bitterness, and aroma. Fining involves the addition of adsorptive or reactive material in order to reduce or eliminate the presence of certain less desirable wine components and to ensure that a wine remains in a particular stable state for a given period of time Recently concerns have been raised about the addition of animal proteins, such as gelatin, to wine due to the disease known as bovine spongiform encephalopathy (Mad Cow disease). Although the origin of gelatins has been moved to porcine, winemakers are asking for substitute products with properties and application protocols similar to the traditional animal-derived ones, making the use of plant-derived proteins in fining a practically viable possibility. As a consequence, various fining agents derived from plants have been proposed, including proteins from cereals, legumes, and potato.

Reduction of herbaceous aromas by wine lactic acid bacteria mediated degradation of volatile aldehydes

Consumers typically prefer wines with floral and fruity aromas over those presenting green-pepper, vegetal or herbaceous notes. Pyrazines have been identified as causatives for herbaceous notes in wines, especially Bordeaux reds. However, pyrazines are not universally responsible for herbaceousness, and several other wine volatile compounds are known to produce distinct vegetal/herbaceous aromas in wines. Specifically, volatile aldehydes elicit sensations of herbaceousness or grassiness and have been described in wines well above their perception thresholds.

Effect of malolactic fermentation in barrels or stainless steel tanks on wine composition. Influence of the barrel toasting

Ellagitannin, anthocyanin and woody volatile composition of Cabernet Sauvignon wines aged in oak barrels for 12 months was evaluated. Depending on the container where malolactic fermentation (MLF) was carried out, two wine modalities were investigated: wines with MLF carried out in stainless steel tanks and barrel-fermented wines. Three toasting methods (medium toast, MT; medium toast with watering, MTAA; noisette) were considered for ageing of each wine modality. Sensory analyses (triangle and rating tests) were also performed. Two-way ANOVA of the raw experimental data revealed that the toasting method and the container where MLF took place, as well as the interaction between both factors, have a significant influence (p < 0.05) on ellagitannin, anthocyanin and woody volatile profiles of Cabernet Sauvignon wines.