Macrowine 2021
IVES 9 IVES Conference Series 9 Comparison of various storage conditions to preserve polyphenols in red-grape pomace

Comparison of various storage conditions to preserve polyphenols in red-grape pomace

Abstract

Red grape pomace, a waste from wine production, can be valorised by extracting polyphenols, high-added value compounds used in cosmetics or oenology. For use at an industrial level, using green extraction techniques, pomace need to be stored before being processed. The aim of this study is to test various storage conditions in order to maintain high level of polyphenols over 180 days, while keeping storage cost economically interesting. In a first step, different storage conditions (ambient temperature or cooled (4°C) temperature, anaerobic (saturation with N2) or aerobic conditions, and addition of sulphur dioxide (SO2)) were compared on small samples (1 kg) packed in plastic pockets. The quality of storage was assessed by following the optical density of the pomace extract at 280 nm (DO 280 expressed as mg/l eq gallic acid), which is an indication of the amount of remaining extractable polyphenols. The Colour Intensity (CI) (DO 420/520) was also measured as an indicator for oxidation. Organic acids (e.g. malic acid), sugars (e.g. glucose) alcohol, bacteria and yeasts were also quantified as an indicator for the occurrence of fermentation processes in the stored pomace. The results show that storage at 4°C under anaerobic conditions provides the best conditions to preserve polyphenols. Only little reduction of the polyphenol concentration, as well as little oxidation were observed. The addition of SO2 alone could not prevent a strong reduction of the polyphenol concentration in samples stored at ambient temperature. The preservation of pomace under ambient temperature, without protection against oxidation (N2 or SO2) induced a high activity of bacteria and yeasts measured by the reduction of sugar contents, and the transformation of alcohol in acetic acid. Even though refrigeration is efficient against polyphenol lost, at industrial scale it is costly. Therefore in a second step, storage under aerobic and anaerobic conditions (saturation with N2) at ambient temperature in opened or close containers were tested on larger size samples (700 l) to evaluate the effect of large volumes on storage. One assumption was that the core of the heap would be protected from oxidation. The results show that temperature measured in the pomace heap at a depth of 20 cm did not differ from temperature measured at 50 cm. It was confirmed by the monitoring of the fermentation processes which were identical at the surface and deep inside the heap. The assumption regarding protection of the heap core against oxidation was thus incorrect. A strong increase of temperature during the 60 first days of storage was observed under aerobic conditions indicating the occurrence of important fermentation processes. The best way to preserve high-added value compounds in large amount of red grape pomaces is to store it in a sealed tank saturated with N2. Under these conditions the fermentation processes were very limited and the polyphenols were protected from oxidation.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Anne-Claire Silvestri*, Jean-Philippe Burdet, Laure Steiner-Convers

*HES-SO

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Analysis of the oenological potentials of different oak forests in Hungary

Like France, Hungary has many oak forests used for making barrels since many years. But if the differences between the woods of the North, the East and the South-West forests of France are well known, this is probably not the case of Hungarian forests. However taking into account the essential differences of climates and soils, differences must be significant and the general name “Hungarian oak” must not have any real meaning. We have studied precisely (determination of concentrations of volatile and non-volatile wood compounds, anatomical criteria, measurement of antioxidant capacity) of oaks collected from northeastern Hungary and others collected from the Danube valley in the northwest of the country.

Accumulation of polyphenols in Barbera and Nebbiolo leaves during the vegetative season

Grapevine berries produce thousands of secondary metabolites of diverse chemical nature that have been largely detailed in the past due to their importance for defining wine quality. The wide Vitis vinifera diversity, resulting in thousands of different varieties well detailed in many studies regarding berries, is still not investigated in vegetative organs, leaves in particular. Deepening knowledge related to this aspect could be of great interest for many reasons (for example the possibility of using leaf extract for pharmaceutical, cosmetic and nutrition purposes) but, above all, for understanding the susceptibility of different grapevine varieties to pathogens.

Development of a new sustainable filtering media for wine and beer clarification and sterilisation

Different separation techniques are frequently used during vinification process. Nowadays, clarification and microbiological stabilization of wine or beer can be done using precoat filters or crossflow filters to remove yeast and bacteria. Kieselguhr powders are the most used filter aids for precoat filtration. Their crystalline structure and their pulverulent nature induce ecotoxicological risks when used. Moreover, regeneration and reuse of these filter aids is not efficient and the filtration waste requires cost effective retreatment.

Assessing the effect of oak derived aromas on mouthfeel perception in Chardonnay wine

Mouthfeel is an important quality parameter for Chardonnay wines, particularly those aged in oak. While research on mouthfeel has traditionally focused on the impact of non-aromatic compounds, the role of aroma compounds has largely been over looked. However, in wine as well as other food interactions between retronasal aroma and mouthfeel have been noted. The goal of this research was to investigate the impact of wine aroma on the perception of mouthfeel. Because of the importance of oak aging in the development of Chardonnay mouthfeel, the impact of oak aromas on perceived mouthfeel was explored. Aroma compounds associated with oak (ethyl palmitate, eugenol, furfural, isoeugenol, syringaldehyde, vanillin and whiskey lactone) were added to two different Chardonnay wines; one with no oak influence and one fermented in neutral oak. Low and high concentrations of the compounds were added based on concentrations typically found in barrel aged Chardonnay wine.

Attractiveness and sweetness of red wines: Synergies between American oak barrels and mannoproteins

In partnership with a Bordeaux property wanting to improve the quality of its second wine, the effects of two factors, American oak barrels and mannoproteins were studied. Their impact on the attractiveness and sweetness of wines were characterized during two successive vintages (2012 and 2013). Vinification took place with a homogeneous batch of Cabernet Sauvignon. The wine was then divided up into various groups of five barrels of French and American oak, new or reused. Analyses of volatile and non-volatile wood compounds were undertaken at four months and eight months of wood ageing, by LC-MS and GC-MS.