terclim by ICS banner
IVES 9 IVES Conference Series 9 Transcriptomic and metabolomic responses to wounding and grafting in grapevine

Transcriptomic and metabolomic responses to wounding and grafting in grapevine

Abstract

Grafting plants uses intrinsic healing processes to join two different plants together to create one functional organism. To further our understanding of the molecular changes occurring during graft union formation in grapevine, we characterized the metabolome and transcriptome of intact and wounded cuttings (with and without buds to represent scions and rootstocks respectively), and homo- and heterografts at 0 and 14 days after wounding/grafting. As over-wintering, dormant plant material was grafted, we also characterized the gene expression changes in the wood during bud burst and spring activation of growth. We observed an asymmetrical pattern of gene expression between above and below the graft interface, auxin and sugar related genes were up-regulated above the graft interface, while genes involved in stress responses were up-regulated below the graft interface. Many genes were differentially expressed between wounded cuttings and homografts, and between the different scion/rootstock combinations. By combining MapMan and gene ontology analysis, we identified several genes families potentially involved in grafting. Our results were consistent with previous work on other plant species, but we were able to identify some specificities linked to grafting in grapevine. By comparing the scion of homo- and hetero-grafts, we also show that grafting with a non-self-rootstock can influence scion gene expression 14 days after grafting. The combination of metabolomics and transcriptomics shows that the changes in gene expression were accompanied by corresponding changes in tissue metabolite concentrations.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Sarah Jane Cookson1*, Grégoire Loupit1#, Virginie Garcia1, Joseph Tran1, Céline Franc3, Gilles De Revel3, Josep Valls Fonayet2,3, Nathalie Ollat1

1 EGFV, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, F-33882, Villenave d’Ornon, France
2 Bordeaux Metabolome Facility, MetaboHUB, PHENOMEEMPHASIS, 33140 Villenave dOrnon, France
3 Unité de recherche Œnologie, EA 4577, USC 1366 INRAE, ISVV, Université de Bordeaux, F33882 Villenave d’Ornon France

#current address:Laboratoire d’Ecologie des Hydrosystèmes Naturels et Anthropisés, CNRS, Université Lyon 1, France

Contact the author*

Keywords

scion, rootstock, grafting, callus, transcriptome, metabolite analysis

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Flanan-3-ol compositional changes in red grape berries (Vitis vinifera L. cv Cabernet franc) from two terroirs of the Loire Valley (France)

La quantité et la qualité des flavonoïdes sont des éléments importants de la qualité de la baie. En particulier, les tannins contribuent de manière essentielle aux propriétés spécifiques des vins rouges telles que la couleur, l’astringence et l’amertume. Cependant, leur synthèse et leurs propriétés sont encore mal connues. Ainsi, la

Polysaccharide families of lyophilized extracts obtained from unfermented varietal grape pomaces

The recovery of bioactive compounds from grape and wine by-products is currently an important objective for revaluation and sustainability. Grape pomace is one of the main by-products and is a rich source of some bioactive compounds. The aim of this study was to evaluate the polysaccharide (PS) composition of extracts obtained from pomaces of different white and red grape varieties of Castilla y León. Grape pomaces were obtained after the pressing in the winemaking process.

Vertical cordon training system enhances yield and delays ripening in cv. Maturana Blanca

The growing interest in minority grape varieties is due to their potential for adaptation to global warming and their oenological capabilities. However, the cultivation of these varieties has often been limited due to their low economic efficiency. One such example is Maturana Blanca, a recently recovered and authorized minority grape variety in the DOCa Rioja region, known for its remarkable oenological potential but low productivity. This study aimed to increase the yield of Maturana Blanca by implementing the vertical cordon training system, which allowed for a higher number of buds per plant and an increased cluster count per vine.

Aromas of Riesling wine: impact of bottling and storage conditions

Storage temperature and bottling parameters are among the most important factors, which influence the development of wine after bottling. It is well studied that higher storage temperatures speed up chemical reactions and results in faster wine aging [1,2]. It is also known that higher SO2 level and lower oxygen content provide better protection and longer shelf-life for the wine. At the same time, the mechanisms of chemical transformations of wine aromas during the aging process are not fully understood. In particular, how oxidation reactions contribute to the transformations of varietal aroma compounds.In the present study [3], we investigated the development of Riesling wine depending on a series of bottling conditions, which differed in the free SO2 level in wine (low—13 mg/L, medium—24 mg/L, high—36 mg/L), CO2 treatment of the headspace.

Red wine substituted esters involved in fruity aromatic expression: an enantiomeric approach to understand their sensory impact and their pathway formation

Among red wines ethyl esters, those from short hydroxylated and branched-chain aliphatic acids constitute a family with a particular behavior and sensory importance. They have been previously discussed in the literature [1] and recent studies have established that some of them were strongly involved in of red wines’ fruity aroma [2]. As some among them have an asymmetrical carbon atom, it seemed important to separate their different enantiomers to obtain an accurate assessment of their organoleptic impact. Three chiral esters have been identified, presenting alkyl and/or hydroxyle substituants: ethyl 2-hydroxy-4-methylpentanoate, ethyl 2-methylbutanoate, and ethyl 3-hydroxybutanoate.