terclim by ICS banner
IVES 9 IVES Conference Series 9 Open-GPB 9 Open-GPB-2024 9 Flash - Abiotic interactions 9 Arinto clones tolerant to climate change: in depth transcriptomic study of tolerant and sensitive genotypes

Arinto clones tolerant to climate change: in depth transcriptomic study of tolerant and sensitive genotypes

Abstract

Drought and heat waves deriving from climate change have been affecting grapevine plants and altering wine characteristics in the past years, and effects are expected to get worst. Innovative approaches to address this problem have been undertaken in several varieties, that consist in exploring intravarietal variability to identify genotypes that are tolerant to abiotic stress. Such is the case of the variety Arinto, where an experimental population of 165 clones installed according to a resolvable row-column design with 6 replicates, was scanned for several parameters, including surface leaf temperature (SLT). Linear mixed models were fitted to the data of the traits evaluated, and the empirical best linear unbiased predictors (EBLUPs) of genotypic effects for each trait were obtained as well as the coefficient of genotypic variation (CVG) and broad sense heritability. The genotypes were then ranked according to their level of tolerance to abiotic stress without loss of yield/quality. The results enabled the selection of a group of genotypes with increased tolerance to stress, and also to identify the group of genotypes that was more sensitive. Then, leaves of the ten most tolerant and the ten most sensitive genotypes were sampled for RNAseq analyses. Samples were taken prior to veraison, on conditions of intense environmental stress. This analysis enabled the characterization of the transcriptome of the sensitive and of the tolerant genotypes and several markers of tolerance were identified.

This research was funded by the projects “Conservation and selection of ancient grapevine varieties” (PDR2020-784-042704), “Save the intra-varietal diversity of autochthonous grapevine varieties” (PRR-C05-i03-|-000016), and Fundação para a Ciência e Tecnologia (FCT), through UIDB/04129/2020; Project LEAF Thematic Line Project Clones4ClimateChange.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Luísa Carvalho*1, David Faísca-Silva1, Teresa Pinto2, J. Miguel Costa1, Sara Amâncio1, Antero Martins1,2, Elsa Gonçalves1,2

1LEAF- Linking Landscape, Environment, Agriculture and Food, Associate Laboratory TERRA; Instituto Superior de Agronomia, Universidade de Lisboa, Portugal
2Associação Portuguesa para a Diversidade da Videira – PORVID, Lisboa, Portugal

Contact the author*

Keywords

abiotic stress, molecular markers, RNAseq, tolerant clones, sensitive clones

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Can different green manure fertilizations affect the vine balance and grape quality? First evidence of multi-year study

In the context of sustainable viticulture that implements organic practices to maintain soil fertility, green manuring plays a crucial role due to its ability to stock carbon and nitrogen in soil while supporting biodiversity.

Physicochemical parameters of juices made from different grape varieties in the 2019 and 2020 Harvests of Rio Grande do Sul

This study evaluated the physicochemical parameters of grape juices produced in the serra gaúcha from the 2019 and 2020 harvests. To do this, 43 juice samples were analyzed, and divided into four distinct categories: juices made exclusively from bordô grapes (sb), juices made from bordô and niágara grapes (sbn), juices combining bordô and isabel grapes, and juices made from cuts of several grape varieties.

Grapevine nitrogen dynamics as a function of crop thinning

Context and purpose. Nitrogen (N) is crucial for plant development but is used inefficiently, with only 30–40% of the fertilizer assimilated by crops, leading to significant environmental losses.

Analysis of off flavours in grapes infected with the fungal bunch rot pathogens, Aspergillus, Botrytis and Pencillium

Fungal bunch rots of grapes cause major losses to grape yield worldwide, yet the impact these moulds have on grape and wine quality is not well characterised. We sought to investigate the formation of unwanted volatile compounds of fungal origin in both synthetic grape juice culture media and in inoculated grape berries. Botrytis cinerea, Aspergillus niger, Aspergillus carbonarius, or Pencillium expansum were grown in synthetic grape juice medium and the culture homogenates analysed 4 and 7 days post inoculation. HS-SPME-GC-MS analysis of the culture homogenates 4 days post inoculation demonstrated that each of the fungi examined produced varying quantities of the mushroom or fungus-like aroma compounds, 1-Octen-3-ol, 1-Octen-3-one and 3-Octanone with A. carbonarius producing up to ten times the amounts of all three metabolites per mg of dry mycelium.

The challenge of quality in sulphur dioxide free wines: natural polyphenol alternatives

Sulphur dioxide (SO2) seems indispensable in winemaking because of its properties. However, a current increasing concern about its allergies effects in food product has addressed the international research efforts on its replacement. This supposes a sufficient knowledge of its properties and conditions of use. Several studies compared SO2 properties against new alternatives that are supposed to overcome SO2 disadvantages. Firstly, the state of art on SO2 wine replacements is revised, and secondly, the last promising results using natural enriched polyphenol extracts are shown.