PIWIs’ variation in drought response under semi-controlled conditions 

Grapevine powdery mildew resistance is a key target for grape breeders and grape growers worldwide. The driver of the USDA-NIFA-SCRI VitisGen3 project is completing the pipeline from germplasm identification to QTL to candidate gene characterization to new cultivars to vineyards to consumers. This is a common thread across such projects internationally. We will discuss how our objectives and approaches leverage big data to advance this initiative, starting with genomics and computer vision phenotyping for gene discovery and genetic improvement. To manage and maintain resistances for long-term sustainability, growers will be trained through our nation-wide extension and outreach plan.

Winemakers are increasingly experimenting with new techniques, such as spontaneous fermentation, prolonged yeast contact, higher pH, minimal sulphur dioxid, filtration and clarification or oxidative ageing. Along with this, the risk of microbial spoilage increases, and so the off-flavour mousiness, long time underestimated, is becoming more frequent. Characteristic of the mousy off-flavour is the delayed perception after swallowing the wine. After a few seconds the flavour appears, reminiscent of a dirty mouse cage. There are three known compounds that cause mousy off-flavor: 2-ethyltetrahydropyridine, 2-acetyltetrahydopyridine, and 2-acetylpyrroline. Yeasts such as Dekkera/Brettanomyces and heterofermentative lactic acid bacteria like Lactobacillus hilgardii can release these compounds.

The chemical composition and the sensory characteristics of wine result from dynamic interactions between several factors including grape variety, soil, viticultural techniques, climate conditions, yeasts metabolism, oenological approaches. Recently, Grigg et al.

Mannoproteins (MPs) are released from the yeast cell wall during alcoholic fermentation and aging on the lees, and influence aspects of wine quality such as haze formation and colour stability. Yet, as this is a slow process with microbiological and sensory risks, the exogenous addition of extracted MPs poses an efficient alternative. While Saccharomyces cerevisiae has long been studied as a prominent source for MPs extraction, their structure and composition greatly differ between yeast species. This may influence their behaviour in the wine matrix and subsequent impact on wine properties. However, although wine yeast species other than S. cerevisiae possibly present an untapped source of MPs, they are still ill-characterised in terms of chemical composition and influence on wine.

The geology of the Western Cape testifies to the former existence of a late Precambrian supercontinent, its fragmentation, the closure of an ocean between the South African and South American continental precursors (Kalahari and Rio de la Plata cratons), the accumulation of marine sediments and limestones, and their compression during a collision between these cratons