PIWIs’ variation in drought response under semi-controlled conditions 

Flow cytometry (FCM) is a powerful technique allowing the detection, characterization and quantification of microbial populations in different fields of application (medical environment, food industry, enology, etc.). Depending on the fluorescent markers and specific probes used, FCM provides information on the physiological state of the cell and allows the quantification of a microorganism of interest within a mixed population. For 15 years, the enological sector has shown growing interest in this technique, which is now used to determine the populations present (of interest or spoilage) and the physiological state of microorganisms at the different stages of winemaking.

The grape maturation process is being affected by the consequences of global climate change and, as a result, there is a gap at harvest time between the technological maturity of grapes (mostly the concentration of sugar and acids) and its phenolic quality. Due to this gap, the wines elaborated using those grapes show a non-adequate phenolic composition, which results in defects on its color and astringency characteristics. Astringency is mainly related to the salivary protein precipitation because of the interaction not only with wine flavanols but also with other wine phenolics, such as flavonols or different pigments.

Viticulture and oenology face two major challenges today, climate change and the reduction in the use of inputs. Climate change induces low acidity and microbiologically less stable wines

Quercetin (Q) is present in grape in form of glycosides and as aglycone. These compounds are extracted from grape skins during winemaking. In wines, following the hydrolysis reactions, the amount of quercetin aglycon can exceed its solubility value. Unfortunately, a threshold solubility concentration for quercetin in wine is not easy to determine because it depends on wine matrix (Gambuti et al., 2020).

Sterols are a class of the eukaryotic lipidome that is essential for the maintenance of the cell membrane integrity and their good functionality (Daum et al., 1998).