terclim by ICS banner
IVES 9 IVES Conference Series 9 Selection of beneficial endophytes from Sicilian grapevine germplasm 

Selection of beneficial endophytes from Sicilian grapevine germplasm 

Abstract

The recent expansion of arid areas due to climate change is putting grapevine and the other traditional productions at risk in all Mediterranean countries with a limited availability of fundamental resources such as water. It is possible to improve the resilience of vineyards by developing sustainable agricultural practices based on biological and natural resources such as endophytic microorganisms that colonize inner plant tissues, and which can potentially increase the tolerance to abiotic stresses. A selection of grapevine endophytes was conducted from 2021 to 2023 as part of the PRIMA project PROSIT. In particular, the research aimed to select consortia of endophytic bacteria and/or fungi able to improve the grapevine tolerance to drought. To this aim, vine leaves were harvested during two vegetative seasons, from local varieties subjected to a long-lasting adaptation to arid conditions without regular irrigation. A wide diversity of genera and species belonging to different microbial phyla was isolated on artificial media (NA and PDA) from surface-sterilized grapevine leaves. Selected fungal and bacterial isolates were identified by molecular barcoding based on ITS and 16S rDNA sequences, respectively. Overall, bacterial endophytes were isolated in higher numbers than fungal ones. In 2022, a higher abundance of fungal colonies was isolated at the end of the growing season, suggesting a seasonal dynamic of the microbiota composition. Some of the selected isolates belong to species already known as PGP endophytes of crops, including grapevine. Preliminary in vitro experiments confirmed that those strains can increase the concentration of auxins, ammonium and soluble phosphate.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Dalila Crucitti1*, Irene Doro2, Michela Zottini2, Alessandra Tondello3, Andrea Squartini3, Roberto De Michele1, Francesco Carimi1, Davide Pacifico1

1 IBBR CNR – Institute of Biosciences and Bioresources, via Ugo La Malfa 153, 90146 Palermo
2 Department of Biology, Università degli Studi di Padova, via U. Bassi 58b, 35131 Padova, Italy
3 Department of Agronomy, Animals, Food, Natural Resources, and Environment, DAFNAE Università degli Studi di Padova, Viale dell’Università 16, 35020 Legnaro (PD), Italy

Contact the author*

Keywords

drought stress, culturable endophytes, bacteria, fungi

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Sensory evaluation of ‘Sauvignon blanc’ grapes by a trained panel

The study described the effect of sensory analysis on commercial ‘Sauvignon blanc’ vineyards within the Stellenbosch Wine of Origin District. The sensorial evaluation of the berries was able to give a description of each parcel type and relate it to the cultural practices.

Are my bubbles shrinking? A deeper look at oxygen desorption in wine

In the past decade, there has been an increasing amount of work dedicated to understanding micro-oxygenation in wine.

Global warming effects on grape growing climate zones within the Rioja Appllation (DOCa Rioja) in north Spain

Aims: The aims of this work were (1) to assess the changes in some of the main bioclimatic indices used for climate viticultural zoning within the Rioja Appellation area in the north of Spain between 1950-2014 (60 years), and (2) to carry out a comprehensive sociological evaluation among grapegrowers and winemakers of this region, to better understand the impact of climate change on their activity, their degree of concern about it and the potential adaptation measures they would be willing to adopt to cope with it in future years.

Exploring the impact of NPR3 gene silencing on the interaction between grapevine and mycorrhizal fungi through genome editing

One of the main plant defence mechanisms is the Systemic Acquired Resistance (SAR) mediated by Salicylic Acid (SA). This is a heightened and broad-spectrum immune response initiated by the exposure to pathogens, inducing resistance not only in the infected site, but also throughout the entire plant. It was demonstrated that plant immune system can be regulated by two classes of SA receptors: NONEXPRESSOR OF PR GENES 1 (NPR1) and NPR1-LIKE PROTEIN 3 and 4 (NPR3/NPR4). While NPR1 is required for SA-induction followed by the expression of pathogenesis-related (PR) protein and resistance against pathogens, NPR3/NPR4 serve as transcriptional co-repressors of SA-responsive genes.

Selected ion flow tube mass spectrometry: a promising technology for the high throughput phenotyping of grape berry volatilome

Wine grapes breeding has been concentrating a lot of efforts within the grape research community over the last decade. The quick phenotyping of genotype quality traits including aroma composition remains challenging. Selected Ion Flow Tube Mass Spectrometry (SIFT-MS), a technology first available in 2008 and developing rapidly, could be particularly valuable for this usage. The aims of this study were i) to use SIFT-MS, to analyze the whole volatilome from different grape varieties, ii) to assess the ability of this technology to discriminate varieties according to their grape aroma composition, and iii) to study the stability of SIFT-MS signal over maturation to define a sampling strategy.