terclim by ICS banner
IVES 9 IVES Conference Series 9 Selection of beneficial endophytes from Sicilian grapevine germplasm 

Selection of beneficial endophytes from Sicilian grapevine germplasm 

Abstract

The recent expansion of arid areas due to climate change is putting grapevine and the other traditional productions at risk in all Mediterranean countries with a limited availability of fundamental resources such as water. It is possible to improve the resilience of vineyards by developing sustainable agricultural practices based on biological and natural resources such as endophytic microorganisms that colonize inner plant tissues, and which can potentially increase the tolerance to abiotic stresses. A selection of grapevine endophytes was conducted from 2021 to 2023 as part of the PRIMA project PROSIT. In particular, the research aimed to select consortia of endophytic bacteria and/or fungi able to improve the grapevine tolerance to drought. To this aim, vine leaves were harvested during two vegetative seasons, from local varieties subjected to a long-lasting adaptation to arid conditions without regular irrigation. A wide diversity of genera and species belonging to different microbial phyla was isolated on artificial media (NA and PDA) from surface-sterilized grapevine leaves. Selected fungal and bacterial isolates were identified by molecular barcoding based on ITS and 16S rDNA sequences, respectively. Overall, bacterial endophytes were isolated in higher numbers than fungal ones. In 2022, a higher abundance of fungal colonies was isolated at the end of the growing season, suggesting a seasonal dynamic of the microbiota composition. Some of the selected isolates belong to species already known as PGP endophytes of crops, including grapevine. Preliminary in vitro experiments confirmed that those strains can increase the concentration of auxins, ammonium and soluble phosphate.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Dalila Crucitti1*, Irene Doro2, Michela Zottini2, Alessandra Tondello3, Andrea Squartini3, Roberto De Michele1, Francesco Carimi1, Davide Pacifico1

1 IBBR CNR – Institute of Biosciences and Bioresources, via Ugo La Malfa 153, 90146 Palermo
2 Department of Biology, Università degli Studi di Padova, via U. Bassi 58b, 35131 Padova, Italy
3 Department of Agronomy, Animals, Food, Natural Resources, and Environment, DAFNAE Università degli Studi di Padova, Viale dell’Università 16, 35020 Legnaro (PD), Italy

Contact the author*

Keywords

drought stress, culturable endophytes, bacteria, fungi

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Montpellier vine & wine sciences (M-WineS)

The Occitanie Region is the first vine-growing area in France: 270 000 hectares of vineyard and an annual production of 15 million hectoliters. Its annual income reaches 1 900 million euros, of which 900 million euros in export.The vine and wine sector is facing many issues: inputs reduction, adaptation to climate change, maintaining the production competitiveness, digital tools integration in production and transformation processes, and the production of quality wines meeting the consumer demand.

LARGE-SCALE PHENOTYPIC SCREENING OF THE SPOILAGE YEAST BRETTANOMYCES BRUXELLENSIS: UNTANGLING PATTERNS OF ADAPTATION AND SELECTION, AND CONSEQUENCES FOR INNOVATIVE WINE TREATMENTS

Brettanomyces bruxellensis is considered as the main spoilage yeast in oenology. Its presence in red wine leads to off-flavour due to the production of volatile phenols such as 4-vinylphenol, 4-vinylguaiacol, 4-ethylphenol and 4-ethylguaiacol, whose aromatic notes are unpleasant (e.g. animal, leather, horse or pharmaceutical). Beside wine, B. bruxellensis is commonly isolated from beer, kombucha and bioethanol production, where its role can be described as negative or positive. Recent genomic studies unveiled the existence of various populations.

Measuring elemental sulfur in grape juice in relation to varietal thiol formation in Sauvignon blanc wines.

Aim: Sauvignon blanc displays a range of styles that can include prominent tropical and passionfruit aromas. Both sensory evaluation and chemical analysis have confirmed the above-average presence of ‘varietal thiols’ in the Sauvignon blanc wines from Marlborough, New Zealand.

Characterization and modelling of water flow on vineyard soil. Effect of compaction and grass cover

In the Burgundy vineyard, frequent tractor traffic and management of inter-rows alternating grass cover and chemical weed-control lead to structural soil contrast between row and inter-row. The aim of this study was to characterize and model water flow in relation with topsoil structure modifications induced by these practices. Void ratio of the different soil volumes were determined using bulk density measurements.

Under-row low competitive herbaceous cover: A sustainable alternative to herbicide in vineyards

Weeds are undesirable plants in agroecosystems as they compete with the crop for essential resources such as light, water and nutrients, compromising the final yield and its quality.