A DNA-free editing approach to help viticulture sustainability: dual editing of DMR6-1 and DMR6-2 enhances resistance to downy mildew 

Climate change is challenging viticulture worldwide compromising its sustainability due to warmer temperatures and the increased frequency of extreme events. Grafting Vitis vinifera L.

Consumers and the wine industry tend to agree on the ability of old vines to produce fruit that allows the production of wine of superior character. However, despite past and ongoing research, objective evidence of this point of view is still debated and studies on robust, specifically dedicated plots are scarce. Thus the impact of grapevine age on berry oenological potential and wine quality remains an open question. To try to objectively address the issue, a unique vineyard was established at Geisenheim University, Germany. It was planted in 1971 with cv. Riesling grafted on 5C Teleki. In 1995 and 2012, several rows were uprooted and replanted with the same rootstock/scion combination, resulting in a vineyard with alternate rows of identical plant material, but with different planting dates. The parameters of technical maturity and grape composition at harvest were analyzed during seasons 2014, 2015, 2016 and 2017 combining HPLC and enzymatic methods. Separate micro-vinifications were made for each age group and wine composition was analyzed by a combination of 1H-NMR and SPE-GC-MS.

Due to climate change, the occurrence of heatwaves and drought events is increasing, with significant impact on viticulture. Common ways to adapt viticulture to a changing climate include site selection, genotype selection, irrigation management and canopy management. The latter mentioned being for instance source-sink manipulations, such as leaf removal, with the aim to delay ripening.

The cultivated vine (Vitis vinifera) is the main species cultivated in the world to make wine. In 2017, the world wine market represents 29 billion euros in exports, and France contributes 8.2 billion (28%) to this trade, making it a traditional market of strategic importance. Viticulture is therefore a key sector of the French agricultural economy. It is in this context that the nutritional diagnosis of the vine is of real strategic interest to winegrowers. Indeed, the fertilization of the vine is a tool for the winegrower that allows him to influence and regulate the quality of the wine.

In mediterranean agriculture, sustainability and productivity are seriously threatened by climate change and water scarcity. This situation is exacerbated by poor management practices such as excessive use of agrochemicals, overgrazing, and monoculture. The Douro demarcated region (ddr) is an emblematic region, classified world heritage site by UNESCO in 2001. Viticulture is the main agricultural activity in DDR, widely known to produce port wine.