A DNA-free editing approach to help viticulture sustainability: dual editing of DMR6-1 and DMR6-2 enhances resistance to downy mildew 

Franciacorta is a viticultural area which extends in the hills to the South of Iseo lake in Lombardy. It is particularly famous for the production of sparkling wines obtained mostly from Chardonnay and Pinot blanc and noir grapes. The name of this territory is of medieval origin and appeared for the first time in 1277 as “Franzacurta”, from the Latin “franchae curtes”, i.e. “tax-free” monasteries. It was geographically delimited in 1429, when it was a territory of the Republic of Venezia.

Certain yeast species belonging to the Pichia genus are known to form a distinctive film on grape must and wine. In a mixed-culture type fermentation, Pichia spp. (P. kluyveri in particular) are known to impart beneficial oenological attributes. In this study, we report on an easy isolation method of Pichia spp. from grape must by exploiting their film-forming capacity on media containing 10% ethanol. We isolated and identified two Pichia species, namely Pichia kudriavzevii and Pichia kluyveri, and subsequently co-inoculated them with Saccharomyces cerevisiae to ferment Gewürztraminer musts. Noteworthy differences included a significant increase in the 2-phenethyl acetate levels with the P. kluyveri co-fermentation and a general increase in ethyl esters with the P. kudriavzevii co-fermentation. Both Pichia co-inoculations yielded higher levels of glycerol in the final wines. Based on all the wine parameters we tested, the P. kluyveri strain that was isolated performed similarly to a commercial P. kluyveri strain.

The vital role of soils in supporting life on our planet cannot be overstated. Soils provide numerous ecosystem services and functions, including biomass production, carbon sequestration, physical support, biological habitat, and genetic reserve, among others. Understanding the characteristics and sensitivity of soils in a specific terroir, along with effective soil management practices, is crucial for the sustainable management of natural resources.

The content of this communication arises from the deliberations of a working group mandated within the framework of the INRA-INAO 2000-2003 research convention, which brought together INAO representatives and researchers who had worked on AOCs or PGIs, in disciplines from the sphere of the humanities (consumer science, marketing, rural development) and biotechnical sciences (agronomy, animal production science, technology, biochemistry).

Quercetin (Q), a phenolic compound released from grape skins during red wine maceration, has been identified as a source of instability in bottled wines, particularly Sangiovese, due to crystallisation. This phenomenon represents an economic challenge for producers and affects wine clarity and consumer perception.