Functional characterization of grapevine MLO genes to define their roles in Powdery mildew susceptibility by CRISPR/Cas9 genome editing

Marsannay est l’appellation la plus septentrionale de la Côte de Nuits, à la limite Sud de Dijon. Elle regroupe trois villages, Chenôve, Marsannay-la-Côte et Couchey, et constitue l’appellation la plus vaste de la Côte de Nuits avec 315 hectares en A.O.C. Elle possède uniquement des niveaux d’appellations régionales et communales depuis 1987.

Berry maturation in warm areas takes place very early, when temperatures are still high and favorable for carbohydrate synthesis and accumulation in the berries, but not as favorable for maintaining high titratable acidity or low pH, or for increasing berry polyphenol content. Different canopy management techniques have been proven to delay berry maturation at the expense of yield (severe canopy trimming, late spring pruning to induce sprouting of dormant buds, etc.). Minimal pruning delays berry ripening by highly increasing yield and by reducing the leaf area to fruit ratio.

Temperature and yeast nutrition profoundly impact wine quality and sensory attributes by modulating yeast aroma production and release during fermentation. While temperature and nitrogen’s individual effects are well-studied, their combined influence, including nutrient type and addition timing, remains underexplored. hence, this study aimed to investigate the simultaneous effects of these factors on fermentation kinetics, aroma production and sensory profile, particularly in a Chardonnay wine production selected as a quite aromatically neutral base.

Modern agriculture employs large amounts of plastics, such as mulching and greenhouse films, thermal covers, plant protection tubes and tying tape. The latter two types are very common in viticulture. Guard tubes are employed to protect young vines from mechanic and atmospheric damage, whilst polymeric tying tape has replaced natural-origin materials to hold the canopy of vines. Both materials are made on synthetic polymers, which include a range of additives to improve their environmental stability remaining in the environment of vineyards for years. During this time, they are exposed to the range of pesticides (fungicides, insecticides and in a lesser extend herbicides) applied to vines.

Galloylated flavan-3-ols are a class of polyphenolic compounds present in various plants, including grape seeds. These compounds are formed through the condensation of flavan-3-ols, such as catechins, although the precise mechanism by which gallic acid is incorporated into the molecule remains unclear.