Functional characterization of grapevine MLO genes to define their roles in Powdery mildew susceptibility by CRISPR/Cas9 genome editing

The intricate relationship between the necrotrophic pathogen Botrytis cinerea and grape berries (Vitis vinifera spp.) can lead to the development of either the desirable noble rot (NR) or the unfavourable grey rot (GR), depending on the prevailing weather conditions.

The use of soil conservation practices in wine grape production is becoming common throughout the world in response to an increased awareness of the value of soil health to maintain crop productivity and environmental quality. However, little information is available on the meaning of soil health within a viticultural context, and what soil properties should be targeted to achieve both the agronomic and environmental goals of wine grape producers. Conservation practices lead to increases in soil organic matter which may improve soil water retention, and increase soil C content therefore constituting a potential avenue to adapt to droughts and sequester C. Well-known management practices such as the use of cover crops, compost or no-till, although effective, seem to result in highly variable outcomes in soil organic matter and other soil health indicators. This variability is likely associated to the application of the practices in different soils and climates. Thus, integration of soil health building practices needs a thorough understanding of their efficacy under different conditions. Furthermore, additions of soil organic matter could trigger emissions of CO2 and N2O, a potent greenhouse gas that could represent a potential tradeoff of soil conservation practices. Finally, nutrient and water availability may be affected by the increase in soil organic matter having consequences for vine balance and grape quality.

This work aimed to evaluate the evolution of phenolic compounds during white winemaking process up to bottling and 12 months storage, together with the influence of different antioxidant strategies

Grape pomace (GP) is a winemaking by-product particularly rich in (poly)phenols and dietary fiber, which are the main active compounds responsible for its health-promoting effects. GP-derived products have been proposed to manage cardiovascular risk factors, including endothelial dysfunction, inflammation, hypertension, hyperglycemia, and obesity. Studies on the potential impact of GP on gut health are much more recent. However, it is suggested that, to some extent, this activity of GP as a cardiometabolic health-promoting ingredient would begin in the gastrointestinal tract as GP components (i.e., (poly)phenols and fiber) undergo extensive catabolism, mainly by the action of the intestinal microbiota, that gives rise to low-molecular-weight bioactive compounds that can be absorbed and utilized by the body.

AIM: Biofilm is a resistance mechanism deployed by microorganisms to adapt to stresses, leading to their persistence in the environment. In the case of Brettanomyces bruxellensis, a wine spoilage yeast, knowledge about its capacity to form biofilm remains limited although this potential strategy could explain its recurring presence in cellars.