Functional characterization of grapevine MLO genes to define their roles in Powdery mildew susceptibility by CRISPR/Cas9 genome editing

Mannoproteins (MPs) with different structure of their polysaccharide part (branching, substitutions, …) were used to better understand the impact of characteristics of the usual structure of MPs when interacting with Grape Seed Tannins (ST). 

AIM: Vitis vinifera L. cv. Moscato includes different varieties, mainly white grapes with a medium-sized berry, spheroidal or slightly flattened in shape, yellow greenish color which becomes golden yellow or amber when exposed to the sun. Moscato varieties are mainly used for the production of sweet aromatic wines

Grapevine downy mildew, caused by the oomycete Plasmopara viticola, is responsible for significant economic losses each year and for a large proportion of the fungicides used in viticulture.

The current levels of greenhouse gas emissions are expecting to provoke a change on the environmental conditions which, among others, will include a rise of global mean surface temperature and an increment of atmospheric CO2 levels (IPCC, 2014), known as climate change. The response of grapevine (Vitis vinifera L.), one of the most important crops in Europe, from both a cultural and economic point of view, is not completely understood yet and the studies considering the interaction between factors are scarce. Besides, the potential variety of responses among somatic variants needs to be studied in order to be exploited in the avoidance of undesired traits linked to climate change (Carbonell‐Bejerano et al., 2015).

How could the wine industry be more sustainable? To answer this, an Interreg French-Swiss project gathered researchers to help a French keg producer and a Swiss wine distributor make their innovation more ecological, social and economical. What innovation? A reusable plastic keg with a disposable airtight pouch inside.