Haplotype-Resolved genome assembly of the Microvine

Bacillus velezensis and Trichoderma harzianum are relevant microorganisms used in viticulture as biocontrol agents against pathogens of trunk (e.g. Phaeoacremonium minimum), leaves (e.g. Plasmopara viticola) or fruit (e.g. Botrytis cinerea), or as biostimulants, improving the resilience of plants against biotic or abiotic stressors through different direct and non-direct interactions.
In this biotechnological approach, formulation plays a crucial role. Controlling water activity in the product, thus stabilising microbial viability is key to ensuring effective application. We present the benefits of the citrate ester CITROFOL® AI (triethyl citrate) as a novel bio-based carrier liquid in microbial formulations. CITROFOL® AI is safe for humans and the environment, thus offering a promising base for sustainable treatments in viticulture.

Between 2002 and 2004 we studied the behaviour of two red grape varieties – Merlot and Cabernet Sauvignon – within the scope of an experimental protocol encompassing 14 plots, 7 of which had not been cultivated, situated in geographically distant locations representing different terroirs of Castilla-La Mancha.

Aims: The aims of this study were to (i) characterize bacterial and fungal communities in selected Australian vineyard soils and (ii) determine if the soil microbiome composition and diversity varied between different zones within a vineyard. 

Environmental analysis (climate, vegetation, geomorfoloy-lanscape, lithology and soil) and its integration in a quality index taking the Appellation of Origin as the sole universe are used as general methodology for terroir zoning in Spain (Sotés and Gómez-Miguel, 1986-2005). This methodology is also applied to specific aspects of different Spanish Appellations of Origin (size, distribution and landscape peculiarities and vine occupation index).

Filtration is a critical step in ensuring the clarity and microbial stability of wine prior to bottling. However the process of filtering potentially reduces red wine quality by removing some of the macromolecules that contribute to the texture of the wine. Commercial red wines, Cabernet Sauvignon (CAS) and Shiraz (SHZ), of two vintages and two grades (premium grade wines from the older vintage: CAS13 and SHZ13; and standard grade wines from a younger vintage: CAS14 and SHZ14) were filtered through industrial-scale commercial filtration units prior to bottling. Samples were taken before and after cross-flow filtration, lenticular filters, 0.65 µm and 0.45 µm pore size nylon membrane filters. The concentration and composition of macromolecules, including tannins and polysaccharides, were measured in all samples as well as particle size distribution and wine colour.