Haplotype-Resolved genome assembly of the Microvine

Melatonin is a bioactive compound with antioxidant properties, that has been found in many fermented beverages, such as beer and wine [1]. Indeed, it has been shown that yeast can synthesize melatonin during alcoholic fermentation, although its role inside the cell, as well as the metabolic pathway involved in its synthesis, is still unclear [1]. Recent studies showed that during fermentation, melatonin interacts with different proteins of the glycolytic pathway in both Saccharomyces and non-Saccharomyces yeast, for instance glyceraldehyde 3-phosphate dehydrogenase, pyruvate kinase or enolase [2].

Vine water status was measured on 96 plots of three vines inside a vineyard block of 0.28 ha during three years: 2003, 2004 and 2005. Three physiological indicators were implemented: stem water potential, carbon isotope discrimination measured on grape sugars at ripeness (δ13C) and canopy temperature measured by high resolution remote sensing. For stem water potential, measurements were taken on every single vine of each plot.

Ampelography is aimed at describing the vine according to several characteristics, such as morphology, agronomic aptitudes, technological potential, and genetics. The description of varieties and species of vitis has long been the subject of numerous scientific and technical studies by eminent specialists for a long time, which have led the OIV to publish in 1983 the “descriptor list for grape varieties and vitis species”, a milestone among the OIV worldwide recognised codes.

Malic acid has a strong impact on wine pH and the contribution of fermenting yeasts to modulate its concentration has been intensively investigated in the past. Recent advances in yeast genetics have shed light on the unexpected property of some strains to produce large amounts of malic acid (“acidic strains”) while most of the wine starters consume it during the alcoholic fermentation. Being a key metabolite of the central carbohydrate metabolism, malic acid participates to TCA and glyoxylate cycles as well as neoglucogenesis. Although present at important concentrations in grape juice, the metabolic fate of malic acid has been poorly investigated.

Aims: The aim of the study was to investigate whether organic or biodynamic management have a long-term impact on 1) the microbial biomass and enzymatic activity in the soil, 2) the soil microbial community, 3) flying as well as soil living arthropods and associated fungi.