terclim by ICS banner
IVES 9 IVES Conference Series 9 Macrowine 9 Macrowine 2010 9 Berry development and biosynthesis of grape secondary metabolites 9 Optimization of aroma production in grape cell suspensions induced by chemical elicitor

Optimization of aroma production in grape cell suspensions induced by chemical elicitor

Abstract

Methyl-jasmonate (MeJA) induces the production of at least 25 compounds with sesquiterpene- like mass spectra in “Cabernet sauvignon”. The most effective concentration of MeJA in stimulating the production of sesquiterpenes was found to be 500 µM if added when the cell suspensions had a PCV of 35 %, and 1000 if added when the cell suspensions had a PCV of 70 %. The production of sesquiterpenes quickly decreased with the increase of cell suspension PCV at the moment of MeJA addition. The production of sequiterpenes began 12 hours after the addition of MeJA, and reached a maximum 72 hours after MeJA addition. MeJA also induced a significantly greater amount of proanthocyanidins and stilbenes in the grape cell cultures.

Publication date: July 3, 2024

Issue: Macrowine 2010

Type: Article

Authors

Claudio D’ONOFRIO1, Paul K. BOSS2

1Department of Fruit Science and Plant Protection of Woody Species ‘G. Scaramuzzi’, Fruit Science Section, University of Pisa, Via del Borghetto 80, 56124 Pisa, I.

2CSIRO Plant Industry, PO Box 350 Glen Osmond SA 5064 AUS.

Contact the author*

Keywords

elicitors, methyl-jasmonate, sesquiterpenes

Tags

IVES Conference Series | Macrowine | Macrowine 2010

Citation

Related articles…

Seed phenolics oxidation: development of a new ripening index 

During ripening seed tannins evolve, as demonstrated by the taste and color changes. In this work we tried to develop an objective, easy and fast index, useful for winemakers. In this direction we propose two different spectrophotometric indexes, one related to the molecular structure and tannin subunits linkages, and the other related to the antioxidant properties. Especially the second one gave very interesting and unexpected results.

Aroma accumulation trends during berry development and selection of grape aroma candidate genes suitable for functional characterization

Grape flavour management in the vineyard requires knowledge of the derivation of individual flavour and aroma characteristics and the effects that different concentrations and interactions between these compounds have on flavour potential.

Investigation of VvDXS function and its effects on muscat flavor levels

In the present study the connection between the positional candidate gene VvDXS and muscat flavor was evaluated by investigating the expression profiles in the berries from a Muscat-type cultivar and a neutral cultivar and its nucleotide diversity of full ORF on grapevine accessions.

Chitosan elicits mono-glucosylated stilbene production and release in fed-batch fermentation of grape cells 

In the present study, the optimal conditions of grape (Vitis vinifera cv ‘Barbera’) cell cultures in batch and fed-batch bioreactor processes were studied to specifically improve the production of mono-glucosylated stilbenes.