Exploring high throughput secondary trait phenomics to improve grapevine breeding

The effect of two rootstocks of different drought tolerance (1103 Paulsen and 3309 Couderc) on sap flow, water relations and gas exchange of cv. Xinomavro (Vitis vinifera L.) was investigated during the 2005 season in Naoussa, Greece. Soil was maintained at field capacity for both rootstock treatments until mid July when a restricted water regime was applied by irrigation cutoff. Sap flow diurnals for the Xinomavro-1103P combination showed a rapid decrease of flow after midday, under water stress conditions.

Climate change and an evolving environmental and societal context call for the exploration of disease-resistant and/or drought-adapted grape varieties that meet the demands of consumers and society.

La véraison, stade intermédiaire du développement de la baie de raisin, correspond au début de la maturation. Aux modifications de coloration de la pellicule sont associées une perte de fermeté, une diminution de l’acidité et une augmentation des teneurs en sucres et pigments ainsi que du volume de la baie. Le stade de véraison des cépages blancs reste difficile à apprécier visuellement. Son évaluation par palpation est subjective et donc sujette à caution.

Vitis champinii cultivars Ramsey and Dog-ridge are main choices for rootstocks to adapt viticulture in semi-arid and arid regions thanks to their distinctive tolerance to drought and salinity. However, genetic studies on non-vinifera rootstocks have heavily relied on the grapevine (Vitis vinifera) reference genome, which difficulted the assessment of the genetic variation between rootstock species and grapevines. In the present study, this limitation is addressed by introducing a novo phased genome assembly and annotation of Vitis champinii. This new Vitis champinii genome was employed as reference for mapping RNA-seq reads from the same species under drought and salt stresses, and for comparison the same reads were also mapped to the Vitis vinifera PN40024.V4 reference genome. A significant increase in alignment rate was gained when mapping Vitis champinii RNA-seq reads to its own genome, compared to the Vitis vinifera PN40024.V4 reference genome, thus revealing the expression levels of genes specific to Vitis champinii. Moreover, differences in coding sequences were observed in ortholog genes between Vitis champinii and Vitis vinifera, which therefore challenges previous differential expression analyses performed between contrasting Vitis genotypes on the same gene from the Vitis vinifera genome. Genes with possible implications in drought and salt tolerance have been identified across the genome of Vitis champinii, and the same genomic data can potentially guide the discovery of candidate genes specific from Vitis champinii for other traits of interest, therefore becoming a valuable resource for rootstock breeding designs, specially towards increased drought and salinity due to climate change.

Da anni la ricerca viticola sta orientando le sue attenzioni verso lo studio della vocazionalità degli ecosistemi viticoli, perché fulcro della produttività della vite e qualità dei suoi frutti.